53 results on '"Klaczko LB"'
Search Results
2. Hidden role of mutations in the evolutionary process.
- Author
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Soares AA, Wardil L, Klaczko LB, and Dickman R
- Subjects
- Alleles, Biological Evolution, Mutation, Models, Genetic, Selection, Genetic
- Abstract
Mutations not only alter allele frequencies in a genetic pool but may also determine the fate of an evolutionary process. Here we study which allele fixes in a one-step, one-way model including the wild type and two adaptive mutations. We study the effect of the four basic evolutionary mechanisms-genetic drift, natural selection, mutation, and gene flow-on mutant fixation and its kinetics. Determining which allele is more likely to fix is not simply a question of comparing fitnesses and mutation rates. For instance, if the allele of interest is less fit than the other, then not only must it have a greater mutation rate, but also its mutation rate must exceed a specific threshold for it to prevail. We find exact expressions for such conditions. Our conclusions are based on the mathematical description of two extreme but important regimes, as well as on simulations.
- Published
- 2021
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3. Comparative analysis of adaptive and neutral markers of Drosophila mediopunctata populations dispersed among forest fragments.
- Author
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Batista MRD, Penha RES, Sofia SH, and Klaczko LB
- Abstract
Comparison of adaptive and neutral genetic markers is a valuable approach to characterize the evolutionary consequences of populations living in environments threatened by anthropogenic disturbances, such as forest fragmentation. Shifts in allele frequencies, low genetic variability, and a small effective population size can be considered clear signs of forest fragmentation effects (due to genetic drift) over natural populations, while adaptive responses correlate with environmental variables. Brazilian Atlantic Forest had its landscape drastically reduced and fragmented. Now, several forest remnants are isolated from each other by urban and crop areas. We sampled Drosophila mediopunctata populations from eight forest remnants dispersed on two adjacent geomorphological regions, which are physiognomic and climatically quite distinct. Microsatellite data of inversion-free chromosomes (neutral genetic marker) indicate low structuration among populations suggesting that they were panmictic and greatly influenced by gene flow. Moreover, significant differences in chromosomal inversion frequencies (adaptive genetic marker) among populations and their correlations with climatic and geographical variables indicate that genetic divergence among populations could be an adaptive response to their environment. Nonetheless, we observed a significant difference in inversion frequencies of a population in two consecutive years that may be associated with edge and demographic effects. Also, it may be reflecting seasonal changes of inversion frequencies influenced by great temperature variation due to edge effects. Moreover, the forest fragment size does not affect genetic variation of neutral markers. Our data indicate that despite oscillations in chromosomal inversion frequencies, D . mediopunctata populations from Brazilian Atlantic Forest and their divergence may be driven by adaptive factors to local differences, perhaps because it is a small flying insect easily carried by the wind increasing its migration rates.
- Published
- 2018
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4. Altitudinal distribution of two sibling species of the Drosophila tripunctata group in a preserved tropical forest and their male sterility thermal thresholds.
- Author
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Batista MRD, Rocha FB, and Klaczko LB
- Subjects
- Animals, Biomass, Drosophila genetics, Female, Genetic Speciation, Male, Rainforest, Seasons, Acclimatization, Altitude, Drosophila physiology, Fertility, Hot Temperature adverse effects, Infertility, Male etiology
- Abstract
Variation of ecophysiological traits may help to explain geographic distribution patterns of Drosophila sibling species. Many traits in ectotherms have optimal performance within specific temperature ranges. Altitudinal gradients are potentially informative for characterizing differences of sibling species distributions. We collected two sibling species of the tripunctata group - Drosophila mediopunctata (MPT) and D. unipunctata (UNI) - at eight altitudes (ranging from 593 to 1185m above sea level) located at a continuous Atlantic Rainforest reserve in consecutive years (2009-2011), with two collections at the hot-rainy season and two at the cold-dry season. Mean altitude was significantly different between species and seasons. Their distributions showed a consistent pattern with MPT always occurring at higher altitudes than UNI. A significant correlation was found between altitude and species relative abundance. We characterized the thermal range of fertility, an important fitness component, for each species and found evidence for differential thermal adaptation. Our results suggest that the two species altitudinal distributions and seasonal relative abundances are consistent with their differential thermal adaptations: MPT seems to be adapted to lower temperatures, occupies higher altitudes and occurs at higher relative abundances in the cold-dry season; while UNI tolerates higher temperatures and occurs at lower altitudes and higher relative abundances in the hot-rainy season. However, their thermal ranges overlap at most temperatures, suggesting that additional variables (e.g. habitat choice, competition, differential survival etc.) may also play a role to determine their distribution in the field., (Copyright © 2017. Published by Elsevier Ltd.)
- Published
- 2018
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5. Differential attraction of drosophilids to banana baits inoculated with Saccharomyces cerevisiae and Hanseniaspora uvarum within a Neotropical forest remnant.
- Author
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Batista MR, Uno F, Chaves RD, Tidon R, Rosa CA, and Klaczko LB
- Abstract
Background: Yeasts are a necessary requisite in the diet of most Drosophila species that, in turn, may vector their dispersal in natural environments. Differential attractiveness experiments and the isolation of yeasts consumed by Drosophila may be informative for characterizing this association. Hanseniaspora uvarum is among the most common yeast species isolated from Drosophila crops, with high attractiveness to drosophilids. Saccharomyces cerevisiae has been widely used to collect flies, and it allows broad sampling of almost all local Drosophila species. Pronounced differences in the field concerning Drosophila attractivity to baits seeded with these yeast species have been previously reported. However, few explicit generalizations have been set. Since late fifties, no field experiments of Drosophila attractivity were carried out in the Neotropical region, which is facing shifts in abiotic and biotic factors. Our objective is to characterize preference behavior that mediates the interaction in the wild among Neotropical Drosophila species and yeasts associated with them. We want to set a broad generalization about drosophilids attracted to these yeasts. Here we present the results of a differential attractiveness experiment we carried out in a natural Atlantic Rainforest fragment to assess the preferences of Drosophila species groups to baits inoculated with H. uvarum and S. cerevisiae ., Methods: Both yeast species were cultured in GYMP broth and separately poured in autoclaved mashed banana that was left fermenting. In the field, we collected drosophilids over five arrays of three different baits: non-inoculated autoclaved banana and banana inoculated with each yeast. In the laboratory the drosophilids were sorted to five sets according to their external morphology and/or genitalia: tripunctata ; guarani ; willistoni ; exotic ; and the remaining flies pooled in others ., Results and Conclusions: Uninoculated banana baits attracted virtually no flies. We found significant departures from random distribution over the other two baits (1:1 proportion) for all sets, except the pooled others . Flies of the sets willistoni and exotic preferred H . uvarum over S . cerevisiae, while the remaining sets were more attracted to S . cerevisiae . Previously, various authors reported similar patterns in attraction experiments with S . cerevisiae and H . uvarum . It is also noteworthy that both yeast species have been isolated from natural substrates and crops of Drosophila species. Taken together, these results suggest that the preferences among Drosophila species groups may be reflecting deep and stable relations with yeast species in natural environments. They can be summarized as: forest dwelling species from subgenus Drosophila (such as tripunctata and guarani groups) are attracted to banana baits seeded with S . cerevisiae ; while exotic (as D. melanogaster ) and subgenus Sophophora species are preferentially attracted to baits seeded with H . uvarum ., Competing Interests: The authors declare there are no competing interests.
- Published
- 2017
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6. Phenotypic differentiation in love song traits among sibling species of the Lutzomyia longipalpis complex in Brazil.
- Author
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Vigoder FM, Souza NA, Brazil RP, Bruno RV, Costa PL, Ritchie MG, Klaczko LB, and Peixoto AA
- Subjects
- Animals, Brazil, Copulation, Female, Male, Psychodidae classification, Vocalization, Animal, Psychodidae physiology
- Abstract
Background: Brazilian populations of Lutzomyia longipalpis may constitute a complex of cryptic species, and this report investigates the distribution and number of potential sibling species. One of the main differences observed among Brazilian populations is the type of acoustic signal produced by males during copulation. These copulation song differences seem to be evolving faster than neutral molecular markers and have been suggested to contribute to insemination failure observed in crosses between these sibling species. In previous studies, two main types of copulation songs were found, burst-type and pulse-type. The latter type can, in turn, be further subdivided into five different patterns., Methods: We recorded male song from 13 new populations of the L. longipalpis complex from Brazil and compared the songs with 12 already available., Results: Out of these 25 populations, 16 produce burst-type and 9 produce pulse-type songs. We performed a principal component analysis in these two main groups separately and an additional discriminant analysis in the pulse-type group. The pulse-type populations showed a clear separation between the five known patterns with a high correspondence of individuals to their correct group, confirming the differentiation between them. The distinctiveness of the burst-type subgroups was much lower than that observed among the pulse-type groups and no clear population structure was observed. This suggests that the burst-type populations represent a single species., Conclusion: Overall, our results are consistent with the existence in Brazil of at least six species of the L. longipalpis complex, one with a wide distribution comprising all the populations with burst-type songs, and five more closely related allopatric siblings with different pulse-type song patterns and more restricted distribution ranges.
- Published
- 2015
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7. Chromosomal localization of microsatellite loci in Drosophila mediopunctata.
- Author
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Cavasini R, Batista MR, and Klaczko LB
- Abstract
Drosophila mediopunctata has been used as a model organism for genetics and evolutionary studies in the last three decades. A linkage map with 48 microsatellite loci recently published for this species showed five syntenic groups, which had their homology determined to Drosophila melanogaster chromosomes. Then, by inference, each of the groups was associated with one of the five major chromosomes of D. mediopunctata. Our objective was to carry out a genetic (chromosomal) analysis to increase the number of available loci with known chromosomal location. We made a simultaneous analysis of visible mutant phenotypes and microsatellite genotypes in a backcross of a standard strain and a mutant strain, which had each major autosome marked. Hence, we could establish the chromosomal location of seventeen loci; including one from each of the five major linkage groups previously published, and twelve new loci. Our results were congruent with the previous location and they open new possibilities to future work integrating microsatellites, chromosomal inversions, and genetic determinants of physiological and morphological variation.
- Published
- 2015
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8. Undesirable consequences of neglecting nonlinearity: response to comments by Liefting et al. (2013) on Rocha & Klaczko (2012).
- Author
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Rocha FB and Klaczko LB
- Subjects
- Animals, Drosophila anatomy & histology, Drosophila genetics
- Abstract
In response to our previous study, Liefting et al. argue, in defense of their work on latitudinal variation of developmental-rate reaction norms (RNs), that (1) developmental rate (the reciprocal of development time: rate = time(-1) ) is a more biologically relevant variable than development time; (2) the linear RN model is a valid approximation; and (3) three experimental points suffice to estimate RN parameters. Here, we reply to their comments. First, we give evidence that the complexity of actual development challenges the appealing simplicity of developmental rate. Using the same analysis as Liefting et al. to test their hypothesis with development time, instead of rate, reveals a pattern that is the opposite of their conclusion. Second, we show that a quadratic model is consistent with the whole development-time RNs and explains this contradiction. Third, with the quadratic model, we introduce two parameters to study plasticity: the RN shape (the quadratic coefficient) and RN local plasticity (the derivative of the RN function). The first showed a statistically significant correlation with latitude; and the second showed a continuous variation pattern where all localized patterns can be found (positive, negative, or nonsignificant correlations with latitude) but certainly cannot be generalized., (© 2014 The Author(s). Evolution © 2014 The Society for the Study of Evolution.)
- Published
- 2014
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9. Low temperature reveals genetic variability against male-killing Spiroplasma in Drosophila melanogaster natural populations.
- Author
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Ventura IM, Costa T, and Klaczko LB
- Subjects
- Animals, Drosophila melanogaster physiology, Female, Genetic Variation, Male, Phenotype, Population Dynamics, Sex Ratio, Symbiosis, Drosophila melanogaster microbiology, Spiroplasma genetics, Temperature
- Abstract
Spiroplasma endosymbionts are maternally inherited microorganisms which infect many arthropod species. In some Drosophila species, it acts as a reproductive manipulator, spreading in populations by killing the sons of infected mothers. Distinct Drosophila melanogaster populations from Brazil exhibit variable male-killing Spiroplasma prevalences. In this study, we investigated the presence of variability for the male-killing phenotype among Drosophila and/or Spiroplasma strains and verified if it correlates with the endosymbiont prevalence in natural populations. For that, we analyzed the male-killing expression when Spiroplasma strains from different populations were transferred to a standard D. melanogaster line (Canton-S) and when a common Spiroplasma strain was transferred to different wild-caught D. melanogaster lines, both at optimal and challenging temperatures for the bacteria. No variation was observed in the male-killing phenotype induced by different Spiroplasma strains. No phenotypic variability among fly lines was detected at optimal temperature (23 °C), as well. Conversely, significant variation in the male-killing expression was revealed among D. melanogaster lines at 18.5 °C, probably caused by imperfect transmission of the endosymbiont. Distinct lines differed in their average sex ratios as well as in the pattern of male-killing expression as the infected females aged. Greater variation occurred among lines from one locality, although there was no clear correlation between the male-killing intensity and the endosymbiont prevalence in each population. Imperfect transmission or male killing may also occur in the field, thus helping to explain the low or intermediate prevalences reported in nature. We discuss the implications of our results for the dynamics of male-killing Spiroplasma in natural populations.
- Published
- 2014
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10. Differential occurrence of chromosome inversion polymorphisms among Muller's elements in three species of the tripunctata group of Drosophila, including a species with fast chromosomal evolution.
- Author
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Brianti MT, Ananina G, and Klaczko LB
- Subjects
- Animals, In Situ Hybridization, Fluorescence, Karyotype, Phylogeny, Physical Chromosome Mapping, Polytene Chromosomes ultrastructure, Chromosome Inversion, Drosophila genetics, Evolution, Molecular, Polymorphism, Genetic, Polytene Chromosomes genetics
- Abstract
Detailed chromosome maps with reliable homologies among chromosomes of different species are the first step to study the evolution of the genetic architecture in any set of species. Here, we present detailed photo maps of the polytene chromosomes of three closely related species of the tripunctata group (subgenus Drosophila): Drosophila mediopunctata, D. roehrae, and D. unipunctata. We identified Muller's elements in each species, using FISH, establishing reliable chromosome homologies among species and D. melanogaster. The simultaneous analysis of chromosome inversions revealed a distribution pattern for the inversion polymorphisms among Muller's elements in the three species. Element E is the most polymorphic, with many inversions in each species. Element C follows; while the least polymorphic elements are B and D. While interesting, it remains to be determined how general this pattern is among species of the tripunctata group. Despite previous studies showing that D. mediopunctata and D. unipunctata are phylogenetically closer to each other than to D. roehrae, D. unipunctata shows rare karyotypic changes. It has two chromosome fusions: an additional heterochromatic chromosome pair and a pericentric inversion in the X chromosome. This especial conformation suggests a fast chromosomal evolution that deserves further study.
- Published
- 2013
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11. Connecting the dots of nonlinear reaction norms unravels the threads of genotype-environment interaction in Drosophila.
- Author
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Rocha FB and Klaczko LB
- Subjects
- Abdomen anatomy & histology, Animals, Drosophila growth & development, Genetic Variation, Linear Models, Models, Statistical, Nonlinear Dynamics, Phenotype, Pigmentation, Temperature, Drosophila anatomy & histology, Drosophila genetics
- Abstract
Two contrasting views can characterize the attitude of many studies toward reaction norms (RNs). An "optimistic" view attempts to use a linear model to describe RN variation; and a "pessimistic" view emphasizes RNs complexity without using any model to describe them. Here, we have analyzed the shape of 40 RNs of five traits of Drosophila mediopunctata in response to 11 temperatures. Our results, along with several other studies, show that RNs are typically curves best explained by nonlinear models. Estimating the set of 40 RNs on the basis of three rather than 11 temperatures produces a scenario, typical of the pessimistic view, where the linear model is either nonsignificant or a poor explanatory model. Moreover, we show that RN nonlinearity can significantly affect the conclusions of studies using the linear model. We propose a middle ground view on RNs which recognizes their general nonlinearity. Such view could, on the one hand, explain part of the important phenomenon of genotype-environment interaction emphasized by the pessimistic view. Moreover, it may explain features and patterns which are being ignored by the optimistic view. We suggest the parabolic model as first step to reveal patterns which were ignored before, or not fully appreciated., (© 2012 The Author(s). Evolution© 2012 The Society for the Study of Evolution.)
- Published
- 2012
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12. Spiroplasma in Drosophila melanogaster populations: prevalence, male-killing, molecular identification, and no association with Wolbachia.
- Author
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Ventura IM, Martins AB, Lyra ML, Andrade CA, Carvalho KA, and Klaczko LB
- Subjects
- Animals, Brazil, DNA, Bacterial genetics, DNA, Bacterial isolation & purification, Female, Male, Phylogeny, Polymerase Chain Reaction, Prevalence, Sex Factors, Sex Ratio, Species Specificity, Spiroplasma classification, Spiroplasma isolation & purification, Symbiosis, Wolbachia genetics, Wolbachia isolation & purification, Drosophila melanogaster microbiology, Spiroplasma genetics, Spiroplasma physiology, Wolbachia physiology
- Abstract
Spiroplasma endosymbionts are maternally transmitted bacteria that may kill infected sons resulting in the production of female-biased broods. The prevalence of male killers varies considerably both between and within species. Here, we evaluate the spatial and temporal status of male-killing and non-male-killing Spiroplasma infection in three Brazilian populations of Drosophila melanogaster, nearly a decade after the first occurrence report for this species. The incidence of the male-killing Spiroplasma ranged from close to 0 to 17.7 % (so far the highest estimate for a Drosophila species) with a suggestion of temporal decline in a population. We also found non-male-killing Spiroplasma coexisting in one population at lower prevalence (3-5 %), and we did not detect it in the other two. This may be taken as a suggestion of a spreading advantage conferred by the male-killing strategy. Sequencing two loci, we identified the phylogenetic position of Spiroplasma strains from the three localities, showing that all strains group closely in the poulsonii clade. Due to intensive sampling effort, we were able to test the association between Spiroplasma infections and another widespread endosymbiont, Wolbachia, whose prevalence ranged from 81.8 to 100 %. The prevalence of Wolbachia did not differ between Spiroplasma-infected and uninfected strains in our largest sample nor were the prevalences of the two endosymbionts associated across localities.
- Published
- 2012
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13. Spiroplasma infection in Drosophila melanogaster: what is the advantage of killing males?
- Author
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Martins AB, Ventura IM, and Klaczko LB
- Subjects
- Adaptation, Physiological, Animals, Body Size, Drosophila melanogaster physiology, Energy Metabolism physiology, Female, Genetic Fitness, Host-Pathogen Interactions physiology, Male, Population Density, Sex Factors, Spiroplasma pathogenicity, Drosophila melanogaster microbiology, Reproduction physiology, Sex Ratio, Spiroplasma physiology
- Abstract
Male-killing bacteria are maternally inherited agents that cause death of sons of infected females. Their transmission rate is commonly high but imperfect and also sensitive to different environmental factors. Therefore, the proportion of infected females should be reduced in each generation. In order to explain male-killers spread and persistence in host population, a mechanism resulting in the relative increase of infected females must outweigh the losses caused by the imperfect transmission. The resource release hypothesis states that the males' death results in increased resources available to sibling females which would otherwise be used by their male siblings. Infected females are then expected: to be larger than uninfected females in natural populations; or to have higher viability; or to have shorter development times; or any combination of these outcomes. Here, we tested the resource release hypothesis by measuring body size of infected and uninfected wild-caught Drosophila melanogaster females and carried out other fitness related measures in the laboratory. Wild-caught infected females produced more daughters than uninfected females in their first days in the laboratory. However, although no significant difference in viability was found in a controlled experiment with infected and uninfected flies from a standard laboratory strain, there was a decrease in development time probably mediated by reduced competition. Fitness effects conditioned by the host genetic background are pointed out as a possible explanation for this difference between wild and laboratory flies. Our findings are discussed in the context of the resource advantage hypothesis., (Copyright 2010 Elsevier Inc. All rights reserved.)
- Published
- 2010
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14. Wing morphometry as a tool for correct identification of primary and secondary New World screwworm fly.
- Author
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Lyra ML, Hatadani LM, de Azeredo-Espin AM, and Klaczko LB
- Subjects
- Analysis of Variance, Animals, Body Weights and Measures, Brazil, Diptera anatomy & histology, Female, Linear Models, Male, Species Specificity, Uruguay, Diptera classification, Sex Characteristics, Wings, Animal anatomy & histology
- Abstract
Cochliomyia hominivorax and Cochliomyia macellaria are endemic Neotropical Calliphoridae species. The former causes severe myiasis in hosts while the latter is Sarcosaprophagous, but commonly found as a second invader in wounds. Due to the morphological similarity between them and the potential losses that C. hominivorax represents for cattle breeders, the rapid and correct identification of these two species is very important. In addition to a correct identification of these species, a good knowledge of C. hominivorax biology can be helpful for designing control programs. We applied geometric morphometric methods to assess wing differences between C. hominivorax and C. macellaria and conduct a preliminary analysis of wing morphological variation in C. hominivorax populations. Canonical variate analysis, using wing shape data, correctly classified 100% of the individuals analyzed according to sex and species. This result demonstrates that wing morphometry is a simple and reliable method for identifying C. hominivorax and C. macellaria samples and can be used to monitor C. hominivorax. Both species show sexual dimorphism, but in C. hominivorax it is magnified. We suggest that this may reflect different histories of selection pressures operating on males and females. Significant differences in wing size and shape were obtained among C. hominivorax populations, with little correlation with latitude. This result suggests that wing variation is also a good morphological marker for studying population variation in C. hominivorax.
- Published
- 2010
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15. Photographic map of the polytene chromosomes of Cochliomyia hominivorax.
- Author
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Batista MR, Ananina G, Azeredo-Espin AM, and Klaczko LB
- Subjects
- Animals, Animals, Domestic parasitology, Chromosome Mapping, Female, Male, Myiasis epidemiology, Myiasis genetics, Sex Chromosomes genetics, Tropical Climate, Chromosomes genetics, Diptera genetics, Myiasis veterinary
- Abstract
Cochliomyia hominivorax (Coquerel) (Diptera: Calliphoridae) is one of the most important myiasis-causing flies and is responsible for severe economic losses to the livestock industry throughout the Neotropical region. A polytene chromosome map is an invaluable tool for the genetic analysis and manipulation of any species because it allows the integration of physical and genetic maps. Cochliomyia hominivorax has a diploid number of 12 chromosomes (2n = 12): five pairs of autosomes and one pair of sex chromosomes (XX/XY), which do not polytenize. We created a new photomap of the polytene chromosomes of C. hominivorax describing its five autosomes (chromosomes 2-6). Pupal trichogen cells, which have chromosomes with a high degree of polytenization, were used to elaborate this map. The photomap was made by comparing 20 different nuclei and choosing, for each chromosome segment, the region with the highest resolution. Thus, we present a new photomap of the five autosomes of this species, with a total resolution of 1450 bands.
- Published
- 2009
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16. Complex patterns of genetic variability in populations of the New World screwworm fly revealed by mitochondrial DNA markers.
- Author
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Lyra ML, Klaczko LB, and Azeredo-Espin AM
- Subjects
- Animals, Gene Amplification, Gene Frequency, Genetic Markers, Genetics, Population, Geography, Polymerase Chain Reaction, Polymorphism, Restriction Fragment Length, Screw Worm Infection veterinary, South America, DNA, Mitochondrial genetics, Diptera genetics, Genetic Variation, Screw Worm Infection genetics
- Abstract
Cochliomyia hominivorax (Coquerel) (Diptera: Calliphoridae), the New World screwworm fly, is an important agent of traumatic myiasis, which is endemic in the Neotropical region and which has great economic impact on the development of the livestock industry. International efforts have been aimed at designing programmes to control and eradicate this species from endemic areas. Thorough knowledge of the population genetics of an insect pest is a fundamental component to ensuring the success of a pest management strategy because it enables the determination of an appropriate geographic scale for carrying out effective treatments. This study undertook an analysis of mtDNA polymerase chain reaction-restricted fragment length polymorphism (PCR-RFLP) in 34 populations of C. hominivorax from 10 countries, encompassing almost all the current distribution of the species. Results showed high levels of mitochondrial DNA variability (pi= 2.9%) and a complex pattern of population genetic structure for this species. Significant population structure (Phi st= 0.5234) and low variability were found in Caribbean populations, suggesting that, in general, islands constitute independent evolutionary entities connected by restricted gene flow. By contrast, high variability and low, but significant, differentiation was found among mainland populations (Phi st= 0.0483), which could not be attributed to geographic distance. Several processes may be acting to maintain the observed patterns, with different implications for establishing control programmes.
- Published
- 2009
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17. Molecular phylogeny of the Drosophila tripunctata and closely related species groups (Diptera: Drosophilidae).
- Author
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Hatadani LM, McInerney JO, de Medeiros HF, Junqueira AC, de Azeredo-Espin AM, and Klaczko LB
- Subjects
- Animals, Bayes Theorem, DNA, Mitochondrial genetics, Drosophila classification, Drosophila Proteins genetics, Genes, Insect, Genes, Mitochondrial, Models, Genetic, Sequence Alignment, Sequence Analysis, DNA, Drosophila genetics, Evolution, Molecular, Phylogeny
- Abstract
We suggest a new phylogenetic hypothesis for the tripunctata radiation based on sequences of mitochondrial genes. Phylogenetic trees were reconstructed by parsimony, maximum likelihood and Bayesian methods. We performed tests for hypotheses of monophyly for taxonomic groups and other specific hypotheses. Results reject the monophyly for the tripunctata group whereas monophyly is not rejected for the tripunctata radiation and other specific groups within the radiation. Although most of the basal nodes were unresolved we were able to identify four clusters within the tripunctata radiation. These results suggest the collection of additional data before a proper taxonomic revision could be proposed.
- Published
- 2009
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18. Evolution of the male genitalia: morphological variation of the aedeagi in a natural population of Drosophila mediopunctata.
- Author
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Andrade CA, Vieira RD, Ananina G, and Klaczko LB
- Subjects
- Animals, Crosses, Genetic, Drosophila anatomy & histology, Female, Genetic Variation, Genitalia, Male anatomy & histology, Karyotyping, Male, Models, Genetic, Phenotype, Wings, Animal anatomy & histology, Biological Evolution, Drosophila genetics, Genes, Insect
- Abstract
To investigate the size and shape of the aedeagus of Drosophila mediopunctata, we used basic statistics and geometric morphometrics. We estimated the level of phenotypic variation, natural and laboratory heritability as well as the phenotypic correlations between aedeagus and wing measures. The wing was used as an indicator for both body size and shape. Positive significant correlation was obtained for centroid size of aedeagus and wing for field parents and their offspring reared in the laboratory. Many positive significant phenotypic correlations were found among linear measures of both organs. The phenotypic correlations were few for aedeagus and wing shape. Coefficients of variation of the measures were on average larger in the aedeagus than in the wing for offspring reared in laboratory, but not for flies coming from the field. Significant "natural" heritabilities were found for five linear measures of the aedeagus and only one for the wing. Few significant heritabilities were found for aedeagus and wing shape, mostly ones concerning the uniform components. In an exploratory analysis, we found that inversion DS-PC0 is associated with both uniform and nonuniform components of shape, respectively, in the wing and aedeagus. Our results do not support the lock-and-key hypothesis for the male genitalia evolution, but cannot refute the sexual selection and pleiotropy hypotheses.
- Published
- 2009
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19. The reaction norm for abdominal pigmentation and its curve in Drosophila mediopunctata depend on the mean phenotypic value.
- Author
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Rocha F, Medeiros HF, and Klaczko LB
- Subjects
- Abdomen anatomy & histology, Animals, Phenotype, Pigmentation, Temperature, Drosophila anatomy & histology, Drosophila genetics, Genetic Variation
- Abstract
The idea of a general independence between the phenotypic plasticity and the mean value of a trait is, presently, a consensus. Here, we use the reaction norm of abdominal pigmentation (number of dark spots) of Drosophila mediopunctata in response to temperature, to test this idea. We raised eight strains, bearing two different chromosomal inversions and with varying mean phenotypic values, under 11 temperatures in a thermal gradient to test for predictions concerning mean phenotypic values, chromosomal inversions, and reaction norms. Our results revealed a strong effect of different phenotypic groups and no effect of different karyotypes on reaction norms. Moreover, we found a significant negative correlation between mean phenotypic value and the curvature of the reaction norms, revealing a high dependency of the reaction norm shape on mean phenotypic value. These results clearly reject the idea of genetic independence between mean value and phenotypic plasticity, and may indicate a pattern of correlation, which may include results from other traits and species, with an importance that has not been fully appreciated.
- Published
- 2009
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20. Comparative analysis of the chromosomal positions of rDNA genes in species of the tripunctata radiation of Drosophila.
- Author
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Brianti MT, Ananina G, Recco-Pimentel SM, and Klaczko LB
- Subjects
- Animals, Drosophila classification, Mitosis, Chromosomes, DNA, Ribosomal genetics, Drosophila genetics, Phylogeny
- Abstract
The location of rDNA on chromosomes has been found to be highly variable in different groups of insect families of various orders. Yet, in other insect families the rDNA position is relatively constant. This contrast so far has received limited attention. We investigated the rDNA position on mitotic chromosomes in 18 species of Drosophila, 16 of which are from the tripunctata radiation, subgenus Drosophila, through fluorescent in situ hybridization (FISH). All species showed fluorescent signals only on the sex chromosomes. On the X chromosome a single fluorescent mark, but with variable locations, was found. On the Y, we observed variation both in location and in number of fluorescent marks (from 1 to 5). This constancy of chromosome location, in contrast to the great variability found in other groups, is consistent with the work carried out in other species of Drosophila. This suggests that the presence of a nucleolus organizer region (NOR) on each sex chromosome is probably an ancestral condition in the genus. Moreover, this difference in the variation of rDNA position among groups points out an interesting evolution question, which deserves further study., ((c) 2009 S. Karger AG, Basel.)
- Published
- 2009
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21. Divergent abdominal bristle patterns in two distantly related drosophilids: antero-posterior variations and sexual dimorphism in a modular trait.
- Author
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Araripe LO, Yassin A, Klaczko LB, Moréteau B, and David JR
- Subjects
- Abdomen, Analysis of Variance, Animals, Drosophila melanogaster anatomy & histology, Drosophila melanogaster genetics, Female, Male, Drosophilidae anatomy & histology, Drosophilidae genetics, Genetic Variation, Sex Characteristics
- Abstract
The number of neurosensory bristles on abdominal sternites of Drosophila is a most investigated trait for quantitative genetic studies. However, the developmental pattern expressed on successive segments in both sexes has remained so far a neglected field. We explored three aspects of this general problem with an isofemale line design: comparing two distantly related species, Drosophila melanogaster and Zaprionus indianus, investigating bristle number variation along the antero-posterior axis, and analysing the sexual dimorphism. Antero-posterior variations could be analysed from segment A2 to A7 in females, and A2-A5 in males. In D. melanogaster, males and females showed parallel changes with a consistently lower number in males. In Z. indianus females the number was quite stable along the abdomen, while in males an important antero-posterior increase was found. The sexual dimorphism was further analysed by considering the female-male correlation and the female/male ratio. The results suggest that sternite bristle number is determined by several developmental genetic systems. One is acting along the antero-posterior axis and may be associated to a gradient, since the genetic correlation decreases when more distant segments are compared. Another is acting in the same way on most segments of both sexes, since the female-male genetic correlation is similar between homologous and non-homologous segments. Finally, genes with specific sex effects are acting on A7 in females of both species, and on A5 in Z. indianus males. The overall architecture of female and male abdomen seems to be constrained by the development of reproductive organs. A large difference between species suggests, however, that the sexual dimorphism of abdominal bristle number is not evolutionarily constrained.
- Published
- 2008
- Full Text
- View/download PDF
22. Shape and size variation on the wing of Drosophila mediopunctata: influence of chromosome inversions and genotype-environment interaction.
- Author
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Hatadani LM and Klaczko LB
- Subjects
- Analysis of Variance, Animals, Discriminant Analysis, Drosophila classification, Female, Genotype, Karyotyping, Male, Reproducibility of Results, Sex Differentiation, Temperature, Chromosome Inversion genetics, Drosophila anatomy & histology, Drosophila genetics, Environment, Wings, Animal anatomy & histology
- Abstract
The second chromosome of Drosophila mediopunctata is highly polymorphic for inversions. Previous work reported a significant interaction between these inversions and collecting date on wing size, suggesting the presence of genotype-environment interaction. We performed experiments in the laboratory to test for the joint effects of temperature and chromosome inversions on size and shape of the wing in D. mediopunctata. Size was measured as the centroid size, and shape was analyzed using the generalized least squares Procrustes superimposition followed by discriminant analysis and canonical variates analysis of partial warps and uniform components scores. Our findings show that wing size and shape are influenced by temperature, sex, and karyotype. We also found evidence suggestive of an interaction between the effects of karyotype and temperature on wing shape, indicating the existence of genotype-environment interaction for this trait in D. mediopunctata. In addition, the association between wing size and chromosome inversions is in agreement with previous results indicating that these inversions might be accumulating alleles adapted to different temperatures. However, no significant interaction between temperature and karyotype for size was found--in spite of the significant presence of temperature-genotype (cross) interaction. We suggest that other ecological factors--such as larval crowding--or seasonal variation of genetic content within inversions may explain the previous results.
- Published
- 2008
- Full Text
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23. Grafting the molecular phylogenetic tree with morphological branches to reconstruct the evolutionary history of the genus Zaprionus (Diptera: Drosophilidae).
- Author
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Yassin A, Araripe LO, Capy P, Da Lage JL, Klaczko LB, Maisonhaute C, Ogereau D, and David JR
- Subjects
- Animals, Consensus Sequence, Geography, Likelihood Functions, Time Factors, Drosophilidae genetics, Evolution, Molecular, Phylogeny
- Abstract
A molecular phylogeny for the drosophilid genus Zaprionus was inferred using a mitochondrial (CO-II) and a nuclear (Amyrel) gene using 22 available species. The combined molecular tree does not support the current classification, dubbed phylogenetic, based entirely upon a morphocline of forefemoral ornamentation. For species for which DNA was not available, phylogenetic positioning was only assigned using morphological characters. In order to avoid conflict between DNA and morphology in the combined analyses (supermatrix method), we developed a new method in which few morphological characters were sampled according to an a priori homoplasy assessment on the consensus molecular tree. At each internal node of the tree, a number of synapomorphies was determined, and species with no molecular sequences were grafted thereon. Analogously to tree vocabulary, we called our method 'morphological grafting'. New species groups and complexes were then defined in the light of our findings. Further, divergence times were estimated under a relaxed molecular clock, and historical biogeography was reconstructed under a maximum likelihood model. Zaprionus appears to be of recent origin in the Oriental region during the Late Miocene ( approximately 10 MYA), and colonization of Africa started shortly after ( approximately 7 MYA) via the maritime route of the Indian Ocean Islands. Most of the morphological and ecological diversification took place, later, in Western Africa during the Quaternary cyclic climatic changes. Furthermore, some species became recent invaders, with one, Zaprionus indianus, has successfully invaded South and North America during the last decade.
- Published
- 2008
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24. Inversion polymorphism and a new polytene chromosome map of Zaprionus indianus Gupta (1970) (Diptera: Drosophilidae).
- Author
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Ananina G, Rohde C, David JR, Valente VL, and Klaczko LB
- Subjects
- Animals, Brazil, Chromosome Breakage, Chromosome Mapping, Chromosomes, Drosophilidae classification, Genes, Insect, Genetics, Population, Linkage Disequilibrium, Chromosome Inversion, Drosophilidae genetics, Polymorphism, Genetic
- Abstract
Zaprionus indianus is a recent invader in Brazil and was probably introduced from the West Afrotropical zone. So far, studies regarding its chromosomal polymorphism were limited to India. We found that Brazilian populations were very different from Indian ones. Five new inversions have been discovered. In(II)A, already described in India, where it is quite common, has also been found in Brazil, where it is very rare. The X-chromosome has three inversions; In(X)Na, In(X)Ke and In(X)Eg, which are frequent in all Brazilian populations studied. In every case, we observed strong linkage disequilibrium among these gene arrangements. During the primary collection period (2001-2002), we noticed a significant positive correlation between the frequency of these inversions and latitude, but this was not confirmed in later investigations. Rearrangement In(IV)EF was also common in all populations, while inversion In(V)B was only found in southern populations. Our data suggest that the founders that recently invaded Brazil were polymorphic for the six inversions observed. The place of origin might be identified more precisely by investigating West African populations. In order to facilitate further investigations, we present an updated polytene chromosome photomap, locating the breakpoints of every inversion observed in Brazilian populations.
- Published
- 2007
- Full Text
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25. Sexual dimorphism of body size and sternopleural bristle number: a comparison of geographic populations of an invasive cosmopolitan drosophilid.
- Author
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David JR, Araripe LO, Bitner-Mathé BC, Capy P, Goñi B, Klaczko LB, Legout H, Martins MB, Vouidibio J, Yassin A, and Moreteau B
- Subjects
- Africa South of the Sahara, Animal Structures anatomy & histology, Animals, Body Size genetics, Drosophila melanogaster anatomy & histology, Drosophila melanogaster genetics, Female, Genetics, Population, Male, Sex Characteristics, South America, Species Specificity, Wings, Animal anatomy & histology, Drosophilidae anatomy & histology, Drosophilidae genetics
- Abstract
Zaprionus indianus is a cosmopolitan drosophilid, of Afrotropical origin, which has recently colonized South America. The sexual dimorphism (SD) of body size is low, males being almost as big as females. We investigated 10 natural populations, 5 from America and 5 from Africa, using the isofemale line technique. Three traits were measured on each fly: wing and thorax length and sternopleural bristle number. Two indices of SD were compared, and found to be highly correlated (r > 0.99). For the sake of simplicity, only the female/male (F/M) ratio was further considered. A significant genetic variability of SD was found in all cases, although with a low heritability (intra-class correlation of 0.13), about half the value found for the traits themselves. For size SD, we did not find any variation among continents or any latitudinal trend, and average values were 1.02 for wing length and 1.01 for thorax length. Bristle number SD was much greater (1.07). Among mass laboratory strains, SD was genetically much more variable than in recently collected populations, a likely consequence of laboratory drift. Altogether, SD, although genetically variable and prone to laboratory drift, is independent of size variations and presumably submitted to a stabilizing selection in nature.
- Published
- 2006
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26. Fitness effects of Wolbachia and Spiroplasma in Drosophila melanogaster.
- Author
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Montenegro H, Petherwick AS, Hurst GD, and Klaczko LB
- Subjects
- Animals, Cell Survival, Extrachromosomal Inheritance, Female, Fertility, Larva growth & development, Male, Drosophila microbiology, Spiroplasma pathogenicity, Symbiosis physiology, Wolbachia pathogenicity
- Abstract
Maternally inherited endosymbionts that manipulate the reproduction of their insect host are very common. Aside from the reproductive manipulation they produce, the fitness of these symbionts depends in part on the direct impact they have on the female host. Although this parameter has commonly been investigated for single infections, it has much more rarely been established in dual infections. We here establish the direct effect of infection with two different symbionts exhibiting different reproductive manipulation phenotypes, both alone and in combination, in the fruit fly Drosophila melanogaster. This species carries a cytoplasmic incompatibility inducing Wolbachia and a male-killing Spiroplasma, occurring as single or double (co-) infections in natural populations. We assessed direct fitness effects of these bacteria on their host, by comparing larval competitiveness and adult fecundity of uninfected, Wolbachia, Spiroplasma and Wolbachia-Spiroplasma co-infected females. We found no effect of infection status on the fitness of females for both estimates, that is, no evidence of any benefits or costs to either single or co-infection. This leads to the conclusion that both bacteria probably have other sources of benefits to persist in D. melanogaster populations, either by means of their reproductive manipulations (fitness compensation from male death in Spiroplasma infection and cytoplasmic incompatibility in Wolbachia infection) or by positive fitness interactions on other fitness components.
- Published
- 2006
- Full Text
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27. Quantitative trait analysis and geographic variability of natural populations of Zaprionus indianus, a recent invader in Brazil.
- Author
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David JR, Araripe LO, Bitner-Mathé BC, Capy P, Goñi B, Klaczko LB, Legout H, Martins MB, Vouidibio J, Yassin A, and Moreteau B
- Subjects
- Animals, Brazil, Drosophila anatomy & histology, Female, Geography, Male, Phenotype, Population Dynamics, Thorax anatomy & histology, Wings, Animal anatomy & histology, Drosophila genetics, Genetics, Population, Quantitative Trait Loci
- Abstract
Five natural samples of a recent South America invader, the drosophilid Zaprionus indianus, were investigated with the isofemale line technique. These samples were compared to five African mainland populations, investigated with the same method. The results were also compared to data obtained on mass cultures of other populations from Africa and India. Three quantitative traits were measured on both sexes, wing and thorax length and sternopleural bristle number. We did not find any latitudinal trend among the American samples, while a significant increase in body size with latitude was observed in the Indian and, to a lesser degree, in the African populations. American populations were also characterized by their bigger size. Genetic variability, estimated by the intraclass correlation among isofemale lines, was similar in American and African populations. The intraline, nongenetic variability was significantly less in the American samples, suggesting a better developmental stability, the origin of which is unclear. A positive relationship was evident between intraline variability of size traits and the wing-thorax length correlation. Altogether, our data suggest that the colonizing propagule introduced to Brazil had a fairly large size, preventing any bottleneck effect being detected. The big body size of American flies suggests that they came from a high-latitude African country. The lack of a latitudinal dine in America seems to be related to the short time elapsed since introduction. The very rapid spread of Z. indianus all over South America suggests that it might rapidly invade North America.
- Published
- 2006
- Full Text
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28. Evolutionary genetics of Drosophila mediopunctata.
- Author
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Klaczko LB
- Subjects
- Animals, Biological Evolution, Drosophila genetics
- Abstract
Drosophila mediopunctata belongs to the tripunctata group, which is the second largest Neotropical group of Drosophila with 64 species described. Here I review the work done with this forest dwelling species, and some applications of the methods developed using it as a model organism, to other species. Specifically I look at: the phylogenetic status of the tripunctata group and its relation with other groups in the Hirtodrosophila-immigrans radiation; D. mediopunctata's chromosome inversion polymorphism (altitudinal cline of frequencies and evidences of selection); the morphological variation of the wing and the development and applications of the ellipse method to describe the morphology of the wing; the variation on the number of aristal branches; the genetic basis of the polychromatism present in D. mediopunctata and its association with chromosome inversions; the sex-ratio trait and its use in the demonstration of Fisher's principle; and, finally, the finding of the transposable P-element in this species.
- Published
- 2006
- Full Text
- View/download PDF
29. Analysis of mitochondrial DNA variability and genetic structure in populations of New World screwworm flies (Diptera: Calliphoridae) from Uruguay.
- Author
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Lyra ML, Fresia P, Gama S, Cristina J, Klaczko LB, and Lima De Azeredo-Espin AM
- Subjects
- Animals, Genetic Variation, Polymerase Chain Reaction, Polymorphism, Restriction Fragment Length, Uruguay, DNA, Mitochondrial genetics, Diptera genetics
- Abstract
The New Word screwworm, Cochliomyia hominivorax (Coquerel 1858) (Diptera: Calliphoridae), is one of the most important insect pests of livestock in the Neotropical region. In this work, polymerase chain reaction-restriction fragment length polymorphism of mitochondrial DNA (mtDNA) was used to study the diversity and population structure of seven geographically distinct populations of C. hominivorax from most of the important livestock areas in Uruguay. The control region (A+T/12S) and subunits 1 and 2 of cytochrome oxidase (cox1/cox2) were amplified and digested with restriction endonucleases. Nine haplotypes were observed among the populations sampled. The mean nucleotide diversity and the haplotype diversity indicated high mtDNA variability in this species. The similarity index, average nucleotide divergence, and analysis of molecular variance results showed no evidence of subpopulation differentiation, indicating that the C. hominivorax populations of Uruguay form a single panmitic population. The distribution pattern of the genetic variation in natural populations of C. hominivorax and the implications of these results for establishing control program are discussed.
- Published
- 2005
- Full Text
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30. Male-killing Spiroplasma naturally infecting Drosophila melanogaster.
- Author
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Montenegro H, Solferini VN, Klaczko LB, and Hurst GD
- Subjects
- Animals, Bacterial Proteins genetics, Base Sequence, Drosophila melanogaster embryology, Male, Phylogeny, Sex Factors, Sex Ratio, Spiroplasma classification, Wolbachia genetics, Drosophila melanogaster microbiology, Spiroplasma physiology
- Abstract
Elucidation of the mechanism of action of selfish genetic elements is difficult outside species with well-defined genetics. Male-killing, the phenomenon whereby inherited bacteria kill male hosts during embryogenesis, is thus uncharacterized in mechanistic terms despite being common and important in insects. We characterized the prevalence, identity and source of the male-killing infection recently discovered in Drosophila melanogaster in Brazil. Male-killing was found to be present in 2.3% of flies from Recife, Brazil, and was uniquely associated with the presence of Spiroplasma infection. The identity of sequences across part of the 16S and across the 16S-23S ITS region indicated that the male-killing infection of D. melanogaster was very closely related to S. poulsonii, the source of the male-killing infection in willistoni group flies also found in South America. The sequences of two further protein-coding genes indicated the D. melanogaster infection to be most closely related to that found in D. nebulosa, from the willistoni group. Our data suggest that the establishment of D. melanogaster in South America was associated with the movement of male-killing bacteria between species.
- Published
- 2005
- Full Text
- View/download PDF
31. Colour polymorphism in Drosophila mediopunctata: genetic (chromosomal) analysis and nonrandom association with chromosome inversions.
- Author
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Hatadani LM, Baptista JC, Souza WN, and Klaczko LB
- Subjects
- Analysis of Variance, Animals, Crosses, Genetic, Female, Male, Sex Chromosomes, Chromosome Inversion, Drosophila genetics, Pigmentation genetics, Polymorphism, Genetic
- Abstract
The presence of three dark spots on the abdomen is typical of the tripunctata group of Drosophila, which is the second largest Neotropical group, with 56 species. In some species, such as D. mediopunctata, the colour pattern varies considerably: ranging from flies showing no spots up to flies with three dark spots. In this paper, we present a genetic (chromosomal) analysis of this character showing that this colour polymorphism is genetically determined mainly by the second chromosome. Since this chromosome is the most polymorphic for inversions in this species, we also examined the influence of the inversions on this character. We used strains in which different second chromosomes were placed on the same genetic background and the offspring between them. We found a nonrandom association between the number of spots and the inversions PA0 and PC0. Thus, our results are consistent with the idea that the factors or genes determining a conspicuous polymorphism are likely to be associated, forming a supergene, and this association would be most efficiently accomplished through a chromosome inversion. Moreover, this is the first time that an association between a conspicuous morphological polymorphism and chromosome inversions has been described.
- Published
- 2004
- Full Text
- View/download PDF
32. Low temperature cure of a male killing agent in Drosophila melanogaster.
- Author
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Montenegro H and Klaczko LB
- Subjects
- Animals, Cytoplasm physiology, Environment, Female, Male, Sex Ratio, Cold Temperature, Drosophila melanogaster parasitology, Parasites pathogenicity
- Abstract
Environmental factors can affect transmission or phenotype expression of selfish cytoplasmic endosymbionts such as embryonic male killers. Temperature is one factor that usually affects the transmission rate of selfish cytoplasmic endosymbionts. Heat cures have been described for several host-parasite systems, cold cures, however, are rare. We report a temperature cure of the Drosophila melanogaster male-killing agent, which occurs when flies are raised at 16.5 degrees C. Flies grown at 20, 24, and 28 degrees C maintained an extremely female biased sexual proportion.
- Published
- 2004
- Full Text
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33. A molecular study of the systematics of the Drosophila tripunctata group and the tripunctata radiation.
- Author
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Yotoko KS, Medeiros HF, Solferini VN, and Klaczko LB
- Subjects
- Animals, Base Sequence, Cluster Analysis, DNA genetics, Databases, Nucleic Acid, Molecular Sequence Data, Sequence Analysis, DNA, Drosophila genetics, Phylogeny
- Published
- 2003
- Full Text
- View/download PDF
34. Polytene chromosome map and inversion polymorphism in Drosophila mediopunctata.
- Author
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Ananina G, Peixoto AA, Souza WN, and Klaczko LB
- Subjects
- Animals, Drosophila Proteins analysis, Female, Chromosome Inversion, Chromosome Mapping, Drosophila genetics, Drosophila Proteins genetics, Polymorphism, Genetic
- Abstract
Drosophila mediopunctata belongs to the tripunctata group, and is one of the commonest Drosophila species collected in some places in Brazil, especially in the winter. A standard map of the polytene chromosomes is presented. The breakpoints of the naturally occurring chromosomal rearrangements are marked on the map. The distribution of breaking points through the chromosomes of D. mediopunctata is apparently non-random. Chromosomes X, II and IV show inversion polymorphisms. Chromosome II is the most polymorphic, with 17 inversions, 8 inversions in the distal region and 9 in the proximal region. Chromosome X has four different gene arrangements, while chromosome IV has only two.
- Published
- 2002
- Full Text
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35. Male-killing selfish cytoplasmic element causes sex-ratio distortion in Drosophila melanogaster.
- Author
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Montenegro H, Souza WN, da Silva Leite D, and Klaczko LB
- Subjects
- Animals, Brazil, Crosses, Genetic, Cytoplasm physiology, Drosophila melanogaster physiology, Female, Male, Sex Ratio, Drosophila melanogaster genetics, Genomic Imprinting, Mutation
- Abstract
Sex ratio distortion induced by a male-killing agent has been found to affect Drosophila melanogaster. The trait was discovered accidentally in a collection of flies from markets in Campinas, São Paulo State, Brazil. Repeated crosses with Canton-S males (for 15 generations to date) and successful transmission using the injection of macerates of sex ratio flies, have shown that the trait is inherited maternally, is cytoplasmic and is infectious. Crosses with strains marked with the visible mutation white and viability experiments at pre-adult stages of development, indicate that the skewed sex ratio results from male mortality before hatching. Males do not transmit the trait to their progeny.
- Published
- 2000
- Full Text
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36. Heritability, phenotypic and genetic correlations of size and shape of Drosophila mediopunctata wings.
- Author
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Bitner-Mathé BC and Klaczko LB
- Subjects
- Animals, Data Interpretation, Statistical, Drosophila anatomy & histology, Female, Male, Phenotype, Drosophila genetics, Genetic Variation, Wings, Animal anatomy & histology
- Abstract
We have studied the morphology of wings of Drosophila mediopunctata employing the ellipse method, a procedure that allows precise descriptions of wing size (SI), wing shape outline (SH), and placement of longitudinal wing veins. We have found that the SH and the points which determine the position of the apices of the third, fourth and fifth longitudinal wing veins show high heritability in nature (the lower bound for the natural heritability is above 0.25). The values found are similar to those obtained for the broad-sense heritabilities (H2) in the laboratory. However, SI and the point which determines the apex of the second longitudinal wing vein showed small lower bounds for heritability in nature, 0.05 and 0.07, respectively, in spite of the high estimates of H2 in the laboratory. These results suggest that size and shape have different genetic properties. We observed a high positive phenotypic correlation between the SH, the fourth and the fifth longitudinal wing veins, which contrasts with a negative correlation between these traits and the second longitudinal vein. That is, as the SH gets longer, the apices of the second and fifth veins become closer to each other. Positive genetic correlations in the field were detected between SH, the fourth and the fifth longitudinal veins and also between the third and the fourth veins.
- Published
- 1999
- Full Text
- View/download PDF
37. Plasticity of Drosophila melanogaster wing morphology: effects of sex, temperature and density.
- Author
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Bitner-Mathé BC and Klaczko LB
- Subjects
- Animals, Drosophila melanogaster genetics, Phenotype, Drosophila melanogaster physiology, Sex Factors, Temperature, Wings, Animal anatomy & histology
- Abstract
In this paper we use an adjusted ellipse to the contour of the wings of Drosophila as an experimental model to study phenotypic plasticity. The geometric properties of the ellipse describe the wing morphology. Size is the geometric mean of its two radii; shape is the ratio between them; and, the positions of the apexes of the longitudinal veins are determined by their angular distances to the major axis of the ellipse. Flies of an inbred laboratory strain of Drosophila melanogaster raised at two temperatures (16.5 degrees C and 25 degrees C) and two densities (10 and 100 larvae per vial) were used. One wing of at least 40 animals of each sex and environmental condition were analyzed (total = 380), a measurement of thorax length was also taken. Wing size variation could be approximately divided into two components: one related to shape variation and the other shape independent. The latter was influenced primarily by temperature, while the former was related to sex and density. A general pattern could be identified for the shape dependent variation: when wings become larger they become longer and the second, fourth and fifth longitudinal veins get closer to the tip of the wing.
- Published
- 1999
- Full Text
- View/download PDF
38. Size and shape heritability in natural populations of Drosophila mediopunctata: temporal and microgeographical variation.
- Author
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Bitner-Mathé BC and Klaczko LB
- Subjects
- Animals, Female, Genetics, Population, Body Constitution genetics, Drosophila genetics, Genetic Variation
- Abstract
'Traditional morphometrics' allows us to decompose morphological variation into its major independent sources, identifying them usually as size and shape. To compare and investigate the properties of size and shape in natural populations of Drosophila mediopunctata, estimating their heritabilities and analysing their temporal and microgeographic changes, we carried out collections on seven occasions in Parque Nacional do Itatiaia, Brazil. In one of these collections, we took samples from five different altitudes. Measurements were taken from wild caught inseminated females and up to three of their laboratory-reared daughters. Through a principal component analysis, three major sources of variation were identified as due to size (the first one) and shape (the remaining two). The overall amount of variation among laboratory flies was about half of that observed among wild flies and this reduction was primarily due to size. Shape variation was about the same under natural and artificial conditions. A genetic altitudinal cline was detected for size and shape, although altitude explained only a small part of their variation. Differences among collections were detected both for size and shape in wild and laboratory flies, but no simple pattern emerged. Shape variation had high heritability in nature, close to or above 40% and did not vary significantly temporally. Although on the overall size heritability (18 +/- 6%) was significant its estimates were not consistent along months--they were non-significant in all but one month, when it reached a value of 51 +/- 11%. Overall, this suggests that size and shape have different genetic properties.
- Published
- 1999
- Full Text
- View/download PDF
39. An experimental demonstration of Fisher's principle: evolution of sexual proportion by natural selection.
- Author
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Carvalho AB, Sampaio MC, Varandas FR, and Klaczko LB
- Subjects
- Alleles, Animals, Biological Evolution, Chromosomes, Female, Genetics, Population, Male, Models, Genetic, Time Factors, Drosophila genetics, Selection, Genetic, Sex Ratio
- Abstract
Most sexually reproducing species have sexual proportions around 1:1. This major biological phenomenon remained unexplained until 1930, when FISHER proposed that it results from a mechanism of natural selection. Here we report the first experimental test of his model that obeys all its assumptions. We used a naturally occurring X-Y meiotic drive system--the sex-ratio trait of Drosophila mediopunctat--to generate female-biased experimental populations. As predicted by FISHER, these populations evolved toward equal sex proportions due to natural selection, by accumulation of autosomal alleles that direct the parental reproductive effort toward the rare sex. Classical Fisherian evolution is a rather slow mechanism: despite a very large amount of genetic variability, the experimental populations evolved from 16% of males to 32% of males in 49 generations and would take 330 generations (29 years) to reach 49%. This slowness has important implications for species potentially endangered by skewed sexual proportions, such as reptiles with temperature sex determination.
- Published
- 1998
- Full Text
- View/download PDF
40. Variation and heritability of aristal morphology in a natural population of Drosophila mediopunctata.
- Author
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Bitner-Mathé BC and Klaczko LB
- Subjects
- Altitude, Animals, Chromosome Inversion, Drosophila anatomy & histology, Female, Gene Rearrangement, Seasons, X Chromosome, Drosophila genetics, Genetic Variation, Sense Organs anatomy & histology
- Abstract
We studied the major sources influencing the variation of the number of aristal branches in a natural population of Drosophila mediopunctata. Flies were collected on six occasions at different altitudes in Parque Nacional do Itatiaia (Brazil). The progenies of these flies were reared in the laboratory at 16.5 degrees C. The number of aristal branches ranges from 11 to 15 and is influenced by sex. Estimates of the natural heritability showed that at least 20% of the total phenotypic variation is due to additive genetic variation. Although the heritability of this trait estimate in the laboratory was larger (42%), the difference between the two estimates is not statistically significant. Thus, for the number of aristal branches, laboratory estimates of heritability provide reasonable estimations of both the magnitude and significance of heritabilities in nature. The mean numbers of aristal branches in the wild-caught flies from different altitudes or months are homogeneous. The same was observed for the means of its progeny kept in the laboratory under controlled conditions. On the other hand, wild-caught females have significantly fewer aristal branches than their laboratory-raised daughters, which suggests that an environmental factor or factors may have an important influence on this trait.
- Published
- 1998
- Full Text
- View/download PDF
41. Polymorphism for Y-linked suppressors of sex-ratio in two natural populations of Drosophila mediopunctata.
- Author
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Carvalho AB, Vaz SC, and Klaczko LB
- Subjects
- Animals, Female, Gene Frequency, Male, Sex Ratio, Drosophila genetics, Polymorphism, Genetic, Y Chromosome
- Abstract
In several Drosophila species there is a trait known as "sex-ratio": males carrying certain X chromosomes (called "SR") produce female biased progenies due to X-Y meiotic drive. In Drosophila mediopunctata this trait has a variable expression due to Y-linked suppressors of sex-ratio expression, among other factors. There are tow types of Y chromosomes (suppressor and nonsuppressor) and two types of SR chromosomes (suppressible and unsuppressible). Sex-ratio expression is suppressed in males with the SRsuppressible/Ysuppressor genotype, whereas the remaining three genotypes produce female biased progenies. Now we have found that approximately 10-20% of the Y chromosomes from two natural populations 1500 km apart are suppressors of sex-ratio expression. Preliminary estimates indicate that Ysuppressor has a meiotic drive advantage of 6% over Ynonsuppressor. This Y polymorphism for a nonneutral trait is unexpected under current population genetics theory. We propose that this polymorphism is stabilized by an equilibrium between meiotic drive and natural selection, resulting from interactions in the population dynamics of X and Y alleles. Numerical simulations showed that this mechanism may stabilize nonneutral Y polymorphisms such as we have found in D. mediopunctata.
- Published
- 1997
- Full Text
- View/download PDF
42. Yeast succession in the Amazon fruit Parahancornia amapa as resource partitioning among Drosophila spp.
- Author
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Morais PB, Martins MB, Klaczko LB, Mendonça-Hagler LC, and Hagler AN
- Subjects
- Animals, Fungi growth & development, Drosophila microbiology, Food Microbiology, Fruit microbiology, Fungi isolation & purification
- Abstract
The succession of yeasts colonizing the fallen ripe amapa fruit, from Parahancornia amapa, was examined. The occupation of the substrate depended on both the competitive interactions of yeast species, such as the production of killer toxins, and the selective dispersion by the drosophilid guild of the amapa fruit. The yeast community associated with this Amazon fruit differed from those isolated from other fruits in the same forest. The physiological profile of these yeasts was mostly restricted to the assimilation of a few simple carbon sources, mainly L-sorbose, D-glycerol, DL-lactate, cellobiose, and salicin. Common fruit-associated yeasts of the genera Kloeckera and Hanseniaspora, Candida guilliermondii, and Candida krusei colonized fruits during the first three days after the fruit fell. These yeasts were dispersed and served as food for the invader Drosophila malerkotliana. The resident flies of the Drosophila willistoni group fed selectively on patches of yeasts colonizing fruits 3 to 10 days after the fruit fell. The killer toxin-producing yeasts Pichia kluyveri var. kluyveri and Candida fructus were probably involved in the exclusion of some species during the intermediate stages of fruit deterioration. An increase in pH, inhibiting toxin activity and the depletion of simple sugars, may have promoted an increase in yeast diversity in the later stages of decomposition. The yeast succession provided a patchy environment for the drosophilids sharing this ephemeral substrate.
- Published
- 1995
- Full Text
- View/download PDF
43. Morphological variation in a natural population of Drosophila mediopunctata: altitudinal cline, temporal changes and influence of chromosome inversions.
- Author
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Bitner-Mathé BC, Peixoto AA, and Klaczko LB
- Subjects
- Animals, Body Constitution, Drosophila genetics, Drosophila physiology, Female, Male, Population Dynamics, Wings, Animal physiology, X Chromosome, Altitude, Chromosome Inversion, Drosophila anatomy & histology, Genetic Variation
- Abstract
To characterize the morphological variation in a natural population of Drosophila mediopunctata, males were collected on three occasions at a single locality. From each wild-caught male 14 body measures were taken and the karyotype for inversions on chromosomes X and II was determined. Through a principal components analysis, two sources of variation, identified as size and shape, accounted for approximately 80 and 6 per cent of the total morphological variability, respectively. The shape component was determined primarily by variations in the position of the wing second longitudinal vein. Differences between collections were detected both for size and shape. An altitudinal cline was observed in respect of wing shape, although altitude explained only a small part of the shape variation. Size and shape were affected by chromosome II inversions. However, in respect of size, no direct differences were detected between karyotypes but a significant interaction between collecting date and karyotype was found. This suggests that karyotypes might differ in their norms of reaction in the field.
- Published
- 1995
- Full Text
- View/download PDF
44. Y-linked suppressors of the sex-ratio trait in Drosophila mediopunctata.
- Author
-
de Carvalho AB and Klaczko LB
- Subjects
- Animals, Female, Genes, Suppressor, Male, Drosophila genetics, Genetic Linkage, Sex Ratio, Y Chromosome
- Abstract
X-linked meiotic drive causing female-biased progenies is known to occur in nine Drosophila species and is called 'sex-ratio'. In D. mediopunctata this trait is associated with the X:21 chromosome inversion and has variable expression. We describe here a powerful Y-linked suppressor system of sex-ratio expression in this species. There are two types of Y chromosomes (suppressor and nonsuppressor) and two types of X:21 chromosomes (suppressible and unsuppressible). Sex-ratio expression is suppressed in males with the 21suppressible/Ysuppressor genotype, whereas the remaining three genotypes produce female-biased progenies.
- Published
- 1994
- Full Text
- View/download PDF
45. Autosomal suppressors of sex-ratio in Drosophila mediopunctata.
- Author
-
de Carvalho AB and Klaczko LB
- Subjects
- Animals, Chromosome Mapping, Crosses, Genetic, Female, Genotype, Male, Drosophila genetics, Sex Ratio, Suppression, Genetic
- Abstract
The sex-ratio trait has been described as the production of progenies with excess of females due to X-linked meiotic drive in the parental males. This trait has a variable expression in Drosophila mediopunctata. We describe here the existence and chromosomal localization of autosomal suppressors of sex-ratio in this species. There are at least four such genes (one on each major autosome) and the strongest effect is localized on chromosome IV. These genes possibly result from the operation of 'Fisher's Principle'; a mechanism of Natural Selection leading to a 1:1 sex ratio.
- Published
- 1993
- Full Text
- View/download PDF
46. Yeasts associated with Drosophila in tropical forests of Rio de Janeiro, Brazil.
- Author
-
Morais PB, Hagler AN, Rosa CA, Mendonca-Hagler LC, and Klaczko LB
- Subjects
- Animals, Brazil, Female, Male, Species Specificity, Tropical Climate, Drosophila microbiology, Yeasts isolation & purification
- Abstract
The distribution and diversity of yeast species vectored by and from the crop of eight species groups of Drosophila is described for two rain forest sites and an urban wooded area in Rio de Janeiro, Brazil. The typical forest Drosophila groups guarani, tripunctata, and willistoni showed a higher diversity of yeasts than the cosmopolitan melanogaster species group, suggesting different strategies of utilization of substrates. Apiculate yeasts, including Kloeckera apis, Kloeckera javanica, and Kloeckera japonica, were the prevalent species. Geotrichum spp. and Candida citea were also frequent isolates in the forest sites. Similarities between the yeasts from the external surfaces and crops of Drosophila suggested that the feeding substrates were the main source of the yeasts vectored by these flies. Most of the yeasts were strong fermenters and assimilated few compounds, usually sucrose, cellobiose, and glycerol. This indicated a preference of the flies for food sources such as fruits. Some yeasts were primarily isolated from one group of Drosophila; for example, Kloeckera javanica from the melanogaster group, Debaryomyces vanrijiae var. yarrowii from the tripunctata group, and Kluyveromyces delphensis from the willistoni group. These associations and differences in the yeast communities among the fly groups suggested a differentiation of diets and specialization of the yeast-Drosophila association in the tropical forests.
- Published
- 1992
- Full Text
- View/download PDF
47. Age and sex-ratio expression in Drosophila mediopunctata.
- Author
-
de Carvalho AB and Klaczko LB
- Subjects
- Animals, Female, Genes, Suppressor, Genotype, Male, Meiosis genetics, X Chromosome, Y Chromosome, Aging genetics, Drosophila genetics, Sex Ratio
- Abstract
The sex-ratio trait--production of progenies with excess of females due to X-linked meiotic drive in parental males--has a variable expression in Drosophila mediopunctata. We tested the effect of male age and found that aging increases the expression of sex-ratio, a fact relevant for the interpretation of field data and for experimental design.
- Published
- 1992
- Full Text
- View/download PDF
48. Linkage disequilibrium analysis of chromosomal inversion polymorphisms of Drosophila.
- Author
-
Peixoto AA and Klaczko LB
- Subjects
- Animals, Chromosome Inversion, Chromosome Mapping, Genetic Linkage, Haplotypes, Phylogeny, Polymorphism, Genetic, Drosophila genetics
- Abstract
The linkage disequilibrium pattern analysis is a method that allows one to detect present and past events of selection. We applied it to our data on the inversions of Drosophila mediopunctata and to published data of Drosophila subobscura, which are systems probably under complex modes of selection. The method gave meaningful results showing that it might be an excellent exploratory tool to identify problems worthy of further study. It is also suggested that it can be used as a criterion to determine the roots of inversion phylogenies.
- Published
- 1991
- Full Text
- View/download PDF
49. Allele frequency estimates when only heterozygotes can be recognized: method of estimation and application in the case of chromosomal inversion polymorphisms in Drosophila.
- Author
-
Klaczko LB, Otto PA, and Peixoto AA
- Subjects
- Alleles, Animals, Drosophila genetics, Heterozygote, Mathematics, Chromosome Inversion, Gene Frequency, Polymorphism, Genetic
- Abstract
A general method to estimate allele frequencies when there are more than two alleles segregating in an autosomal and only the heterozygotes can be identified is presented. The particular three allele case is detailed and the method is applied to chromosomal inversion data of Drosophila mediopunctata. The estimates from egg sample data are compared with those from adult male sample data. No significant differences were found between the two methods. However, the combined use of both data unveiled significant variations in the frequencies of inversions at different collecting times, that otherwise would not have been detected. Furthermore, the method is applied to data on inversion frequencies of D. nebulosa, D. willistoni and D. flavopilosa.
- Published
- 1990
- Full Text
- View/download PDF
50. Reappearance of embryonal antigens in planarian regenerates.
- Author
-
Guimarães JP, Klaczko LB, Hirano K, Vaz EM, and Miguel MR
- Subjects
- Animals, Mice, Antigens analysis, Planarians embryology, Planarians immunology, Regeneration, Turbellaria embryology, Turbellaria immunology
- Abstract
A transient and apparently orderly re-expression of embryonal antigens in planarian regenerates has been demonstrated. This finding seems to corroborate the hypothesis that regeneration in planarians is based on a recapitulation of mechanisms that operate the embryogenesis of these animals, andgives some support to the concept of cancer as a misprogrammed regeneration when contemplated against the background of facts pointed out in the literature.
- Published
- 1975
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