13 results on '"Kizhakke Purayil Anupama"'
Search Results
2. Jatamansinol from Nardostachys jatamansi Ameliorates Tau-Induced Neurotoxicity in Drosophila Alzheimer’s Disease Model
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Kizhakke Purayil Anupama, Anet Antony, Olakkaran Shilpa, Shamprasad Varija Raghu, and Hunasanahally Puttaswamygowda Gurushankara
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Neuroscience (miscellaneous) ,Neurodegenerative Diseases ,tau Proteins ,Antioxidants ,Nardostachys ,Disease Models, Animal ,Cellular and Molecular Neuroscience ,Tauopathies ,Neurology ,Alzheimer Disease ,Animals ,Cholinesterases ,Humans ,Drosophila ,Neurotoxicity Syndromes - Abstract
Nardostachys jatamansi has long been used to prepare Medhya Rasayana in traditional Indian Ayurveda medicine to treat neurological disorders and enhance memory. Jatamansinol from the N. jatamansi against Alzheimer's disease (AD) showed that it could be a multitargeted drug against AD. Drosophila is an ideal model organism for studying a progressive age-related neurodegenerative disease such as AD since its neuronal organizations and functioning are highly similar to that of humans. The current study investigates the neuroprotective properties of jatamansinol against Tau-induced neurotoxicity in the AD Drosophila model. Results indicate jatamansinol is not an antifeedant for larva and adult Drosophila. Lifespan, locomotor activity, learning and memory, Tau protein expression level, eye degeneration, oxidative stress level, and cholinesterase activities were analyzed in 10, 20, and 30-day-old control (wild type), and tauopathy flies reared on jatamansinol supplemented food or regular food without jatamansinol supplementation. Jatamansinol treatment significantly extends the lifespan, improves locomotor activity, enhances learning and memory, and reduces Tau protein levels in tauopathy flies. It boosts the antioxidant enzyme activities, prevents Tau-induced oxidative stress, ameliorates eye degeneration, and inhibits cholinesterase activities in Tau-induced AD model. This study provides the first evidence that jatamansinol protects against Tau's neurotoxic effect in the AD Drosophila model, and it can be a potential therapeutic drug candidate for AD.
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- 2022
3. Present Status and Future Perspectives of Marine Actinobacterial Metabolites
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Puttaswamygowda, Gurushankara Hunasanahally, primary, Olakkaran, Shilpa, additional, Antony, Anet, additional, and Kizhakke Purayil, Anupama, additional
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- 2019
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4. Contributors
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Ankireddy, Seshadri Reddy, primary, Antony, Anet, additional, Balaji, Meriga, additional, Balaji, Hari, additional, Bhargavi, Maram, additional, Chandra, Muni Ramanna Gari Subhosh, additional, Charyulu, P.B.B.N., additional, Cheedarla, Narayanaiah, additional, Dasari, Subramanyam, additional, Deepika, Routhu Gyana, additional, Deepika, Velluri, additional, Divya, Yaramolu, additional, Ganjayi, Muni Swamy, additional, Govindaraju, Saravanan, additional, Gowthami, Krishnamoorthy, additional, Gunturu, Dilip Reddy, additional, Hanna, Luke Elizabeth, additional, Hema, M., additional, Janardhan, Avilala, additional, Jayaraju, Nadimikeri, additional, Karunakaran, Reddy Sankaran, additional, Kathera, Chandrasekhar, additional, Kim, Jongsung, additional, Kizhakke Purayil, Anupama, additional, Koduru, Swetha Kumari, additional, Krishnaveni, M.S., additional, Lakshmi, Bestha, additional, Lee, Min Ho, additional, Madakka, Mekapogu, additional, Madhuri, Ravuri Jaya, additional, Mallikarjuna, Nimgampalle, additional, Mannem, Sravanthi, additional, Mekapogu, Alpha Raj, additional, Murthy, M.R.N., additional, Olakkaran, Shilpa, additional, Paul, Pandeeti Emmanuel Vijay, additional, Puttaswamygowda, Gurushankara Hunasanahally, additional, Rafi, Mokula Mohammed, additional, Rajesh, Nambi, additional, Ramanjaneyulu, Golla, additional, Reddy, Bontha Rajasekhar, additional, Sangeetha, Veeraiah, additional, Saraswathi, Maddu, additional, Saritha, Marella, additional, Savithri, H.S., additional, Sharma, Anshul, additional, Sreenivasulu, Dasari, additional, Sunil Kumar, Myla, additional, Syed, Shameer, additional, Tadepally, Hamsa D., additional, Tollamadugu, N.V.K.V. Prasad, additional, Vishnu Vardhan, G.P., additional, Buddolla, Viswanath, additional, Yegireddy, Muralidhar, additional, Yellamma, Kuna, additional, and Yun, Kyusik, additional
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- 2019
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5. An Outlook on Marine Sponges and Associated Biodiversity Addressing Conservation Strategies
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Kizhakke Purayil Anupama, Anet Antony, Shilpa Olakkaran, Reshma Ramarajan, Shanthala Mallikarjunaiah, and Hunasanahally Puttaswamygowda Gurushankara
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- 2023
6. Jatamansinol from Nardostachys jatamansi: a multi-targeted neuroprotective agent for Alzheimer’s disease
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Anet Antony, Hunasanahally Puttaswamygowda Gurushankara, Kizhakke Purayil Anupama, and Olakkaran Shilpa
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Drug ,education.field_of_study ,biology ,Drug discovery ,business.industry ,media_common.quotation_subject ,Population ,Nardostachys jatamansi ,General Medicine ,Pharmacology ,biology.organism_classification ,Neuroprotection ,Pyranocoumarins ,Structural Biology ,GSK-3 ,Medicine ,education ,business ,Molecular Biology ,Butyrylcholinesterase ,media_common - Abstract
Alzheimer's disease (AD) is a multifactorial progressive and irreversible neurodegenerative disorder characterized by severe memory impairment and cognitive disability in the middle and old-aged human population. There are no proven drugs for AD treatment and prevention. In Ayurveda, medhya plants are used to prepare Rasayana, and its consumption improves memory and cognition. Nardostachys jatamansi (D.Don) DC is a medhya plant used in traditional medicine to treat neurological disorders, and its unique pyranocoumarins can be a potential drug candidate for AD. Given its traditional claims, this study aims to find the multi-target potential efficacy of the ligands (drug molecules) against the AD from N. jatamansi pyranocoumarins using computational drug discovery techniques. Drug likeliness analysis confirms that pyranocoumarins of N. jatamansi, such as seselin, jatamansinol, jatamansine, jatamansinone, and dihydrojatamansin are probable drug candidates for AD. Molecular docking, molecular dynamic simulations, and Molecular Mechanics/Generalized Born Surface Area (MM-GBSA) analysis confirm that dihydrojatamansin inhibits acetylcholinesterase (AChE), and jatamansinol inhibits butyrylcholinesterase (BuChE), glycogen synthase kinase 3β (GSK3β), and kelch-like ECH-associating protein 1 (Keap1) AD therapeutic targets. Therefore, this study provides potential multi-target inhibitors that would further validate experimental studies, leading to new treatments for AD.Communicated by Ramaswamy H. Sarma.
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- 2021
7. Correction to: Lead (Pb)-induced oxidative stress mediates sex-specific autistic-like behaviour in Drosophila melanogaster
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Hunasanahally Puttaswamygowda Gurushankara, Anet Antony, Olakkaran Shilpa, and Kizhakke Purayil Anupama
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Cellular and Molecular Neuroscience ,Neurology ,biology ,Neuroscience (miscellaneous) ,medicine ,Drosophila melanogaster ,biology.organism_classification ,medicine.disease_cause ,Sex specific ,Oxidative stress ,Cell biology - Published
- 2021
8. Lead (Pb)-induced oxidative stress mediates sex-specific autistic-like behaviour in Drosophila melanogaster
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Anet Antony, Hunasanahally Puttaswamygowda Gurushankara, Olakkaran Shilpa, and Kizhakke Purayil Anupama
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Male ,Neuroscience (miscellaneous) ,Biology ,medicine.disease_cause ,Antioxidants ,Superoxide dismutase ,Cellular and Molecular Neuroscience ,Neurodevelopmental disorder ,Sex Factors ,mental disorders ,medicine ,Animals ,Autistic Disorder ,Drosophila ,Genetics ,Gene knockdown ,Behavior, Animal ,Superoxide Dismutase ,fungi ,medicine.disease ,biology.organism_classification ,Catalase ,Oxidative Stress ,Drosophila melanogaster ,Neurology ,Lead ,Autism spectrum disorder ,biology.protein ,Autism ,Female ,Oxidative stress - Abstract
Autism spectrum disorder (ASD) is a highly prevalent neurodevelopmental disorder characterised by three main behavioural symptoms: abnormal social interaction, verbal and non-verbal communication impairments, and repetitive and restricted activities or interests. Even though the exact aetiology of ASD remains unknown, studies have shown a link between genetics and environmental pollutants. Heavy metal lead (Pb), the environmental pollutant, is associated with ASD. Pb may also exhibit sex-specific ASD behaviour, as has been demonstrated in the global human populations. Drosophila melanogaster as a model has been used in the present study to understand the involvement of Pb-induced oxidative stress in developing ASD behaviour. The larval feeding technique has been employed to administer different Pb concentrations (0.2–0.8 mM) to Oregon-R (ORR), superoxide dismutase (Sod), or catalase (Cat) antioxidants overexpressed or knockdown flies. Adult Drosophila (5-day old) were used for Pb content, biochemical, and behavioural analysis.Pb accumulated in the Drosophila brain induces oxidative stress and exhibited a human autistic-like behaviour such as reduced climbing, increased grooming, increased social spacing, and decreased learning and memory in a sex-specific manner.Pb-induced autistic-like behaviour was intensified in Sod or Cat-knockdown flies, whereas Sod or Cat-overexpressed flies overcome that behavioural alterations. These results unequivocally proved that Pb-induced oxidative stress causes ASD behaviour of humans in Drosophila. Thus, Drosophila is used as a model organism to analyse ASD-like human behaviour and underlines the importance of using antioxidant therapy in alleviating ASD symptoms in children.
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- 2021
9. Lead (Pb) induced Oxidative Stress as a Mechanism to Cause Neurotoxicity in Drosophila melanogaster
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Kizhakke Purayil Anupama, Anet Antony, Olakkaran Shilpa, and Hunasanahally Puttaswamygowda Gurushankara
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Male ,Antioxidant ,DNA damage ,medicine.medical_treatment ,Apoptosis ,Toxicology ,medicine.disease_cause ,Antioxidants ,Superoxide dismutase ,Lipid peroxidation ,chemistry.chemical_compound ,medicine ,Animals ,chemistry.chemical_classification ,Reactive oxygen species ,Dose-Response Relationship, Drug ,biology ,Neurotoxicity ,Brain ,Catalase ,medicine.disease ,Cell biology ,Molecular Docking Simulation ,Oxidative Stress ,Drosophila melanogaster ,Lead ,chemistry ,Gene Knockdown Techniques ,biology.protein ,Female ,Neurotoxicity Syndromes ,Lipid Peroxidation ,Reactive Oxygen Species ,Oxidative stress ,DNA Damage - Abstract
The widespread use of lead (Pb) has caused global contamination, inevitable human exposure, and public health problems. Pb neurotoxicity has been linked to various human diseases, but its associated mechanism causing neurotoxicity is unknown. Drosophila melanogaster as a model organism has been used to study the mechanism involved in Pb-caused neurotoxicity and the potential role of antioxidants in ameliorating its harmful effects. The larval feeding technique was adopted to administer different concentrations of Pb (0.2-0.8 mM) to Oregon-R (ORR), superoxide dismutase (Sod), or catalase (Cat) overexpressing, and Sod or Cat knockdown flies to analyse Pb load, oxidative stress components, DNA damage, apoptosis and vacuolation in the brain. The results revealed that Pb accumulation in the Drosophila brain induces oxidative stress by generating reactive oxygen species (ROS) and lipid peroxidation (LPO), depleting antioxidant enzymes. Molecular docking studies have evidenced it. Pb directly binds to antioxidants and major grooves of DNA, leading to DNA damage. Increased DNA damage, apoptosis, vacuolation in brains of Pb-treated ORR, Sod, or Cat knockdown flies; and on the contrary, reduced oxidative DNA damage, apoptosis, and vacuolation in brains of Pb treated Sod or Cat overexpressed flies put forward that oxidative stress is the mechanism in Pb caused neurotoxicity.
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- 2021
10. Oxidative stress-mediated genotoxicity of malathion in human lymphocytes
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Olakkaran, Shilpa, primary, Kizhakke Purayil, Anupama, additional, Antony, Anet, additional, Mallikarjunaiah, Shanthala, additional, and Hunasanahally Puttaswamygowda, Gurushankara, additional
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- 2020
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11. Malathion induced cancer-linked gene expression in human lymphocytes
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Olakkaran Shilpa, Kizhakke Purayil Anupama, Anet Antony, Ramakrishnan Anjitha, Shanthala Mallikarjunaiah, and Hunasanahally Puttaswamygowda Gurushankara
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Microarray ,010501 environmental sciences ,Biology ,medicine.disease_cause ,01 natural sciences ,Biochemistry ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Neoplasms ,Gene expression ,medicine ,Cytotoxic T cell ,Humans ,030212 general & internal medicine ,Lymphocytes ,Pesticides ,Child ,Carcinogen ,0105 earth and related environmental sciences ,General Environmental Science ,Organophosphate ,Cancer ,medicine.disease ,chemistry ,Gene Expression Regulation ,Cancer research ,Malathion ,Carcinogenesis - Abstract
Background Malathion is the most widely used organophosphate pesticide in agriculture. Increasing cancer incidence in agricultural workers and their children links to the exposure of malathion. Identification of genes involved in the process of carcinogenesis is essential for exploring the role of malathion. The alteration in gene expression by malathion in human lymphocytes has not been explored yet, although hematological malignancies are rampant in humans. Objective This study investigates the malathion induced expression of cancer associated genes in human lymphocytes. Methods Human lymphocyte viability and colony-forming ability were analyzed in malathion treated and control groups. Gene expression profile in control and malathion treated human lymphocytes were performed using a microarray platform. The genes which have significant functions and those involved in different pathways were analyzed using the DAVID database. Differential gene expression upon malathion exposure was validated by quantitative real-time (qRT)-PCR. Results Malathion caused a concentration-dependent reduction in human lymphocyte viability. At low concentration (50 μg/mL) of malathion treatment, human lymphocytes were viable indicating that low concentration of malathion is not cytotoxic and induces the colony formation. Total of 659 genes (15%) were up regulated and 3729 genes (85%) were down regulated in malathion treated human lymphocytes. About 57 cancer associated genes related to the growth and differentiation of B and T cells, immunoglobulin production, haematopoiesis, tumor suppression, oncogenes and signal transduction pathways like MAPK and RAS were induced by malathion. Conclusion This study evidences the carcinogenic nature of malathion. Low concentration of this pesticide is not cytotoxic and induces differentially regulated genes in human lymphocytes, which are involved in the initiation, progression, and pathogenesis of cancer.
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- 2019
12. Lead modulated Heme synthesis inducing oxidative stress mediated Genotoxicity in Drosophila melanogaster
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Olakkaran, Shilpa, primary, Antony, Anet, additional, Kizhakke Purayil, Anupama, additional, Tilagul Kumbar, Siddanna, additional, and Hunasanahally Puttaswamygowda, Gurushankara, additional
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- 2018
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13. Oxidative stress-mediated genotoxicity of malathion in human lymphocytes.
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Olakkaran S, Kizhakke Purayil A, Antony A, Mallikarjunaiah S, and Hunasanahally Puttaswamygowda G
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- Acetylcysteine pharmacology, Antioxidants pharmacology, Catalase antagonists & inhibitors, Catalase metabolism, Comet Assay methods, Cytotoxins antagonists & inhibitors, Female, Glutathione Transferase antagonists & inhibitors, Glutathione Transferase metabolism, Humans, Insecticides antagonists & inhibitors, Lipid Peroxidation drug effects, Lymphocytes metabolism, Malathion antagonists & inhibitors, Male, Oxidants antagonists & inhibitors, Oxidative Stress drug effects, Primary Cell Culture, Reactive Oxygen Species agonists, Reactive Oxygen Species metabolism, Superoxide Dismutase antagonists & inhibitors, Superoxide Dismutase metabolism, Young Adult, Cytotoxins toxicity, Insecticides toxicity, Lymphocytes drug effects, Malathion toxicity, Mutagens toxicity, Oxidants toxicity
- Abstract
Applying the single-cell gel electrophoresis (comet) assay, we show that the widely used organophosphorus pesticide malathion is cytotoxic, genotoxic, and induces oxidative stress in human lymphocytes., Competing Interests: Declaration of Competing Interest None declared., (Copyright © 2020 Elsevier B.V. All rights reserved.)
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- 2020
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