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Introduction: Understanding coronophobia, or the heightened fear and anxiety related to the COVID-19 pandemic, involves assessing physiological, cognitive, and behavioral measures. Objectives: We aimed to develop a Greek version of the Coronavirus Anxiety Scale (CAS), the Obsession with COVID-19 Scale (OCS), and the Coronavirus Reassurance-Seeking Behaviors Scale (CRBS), to identify groups that appear vulnerable to this form of pandemic-related anxiety. Methods: We conducted a cross-sectional online study from February to April 2021 in Greek-speaking people living in Cyprus. Participants completed sociodemographic questions and questions related to COVID-19, the CAS, OCS, and the CRBS. All three scales are rated on a 5-point scale, from 0 (not at all) to 4 (nearly every day). For CAS, a score ≥ 9 indicates probable dysfunctional coronavirus-related anxiety, for OCS a ≥ 7 score indicates probable dysfunctional thinking about COVID-19, and for CRBS score ≥ 12 suggests above-average reassurance-seeking activity. Results: A total of 405 adults (66.4% women) from Cyprus participated in this study. The results of this study demonstrate that these Greek adapted measures have adequate reliability (Cronbach's alphas >0.70) and factor structure (exploratory and confirmatory factor analysis support). However, only the CAS demonstrated both convergent and divergent validity. Education personnel, housekeepers, and older adults were also found to have higher coronavirus anxiety relative to their counterparts. Conclusions: The findings of this research support the use of these coronaphobia scales in Cyprus and other Greek-speaking populations. Assessing the potential for fear-driven behaviors may be of great benefit to both clinicians and researchers, helping to identify individuals at risk, adapt interventions, and improve our understanding of the psychological consequences of surviving a public health emergency. Disclosure of Interest: None Declared [ABSTRACT FROM AUTHOR]

Introduction: Humanitarian workers (HWs) face significant challenges while providing aid to those in need, often leading to psychological exhaustion and the risk of primary or secondary trauma. Objectives: Our study aimed to validate and adapt the Greek version of the Brief Resilient Coping Scale (BRCS) for HWs in Greece. Methods: We conducted a cross-sectional study between September and December 2022. Participants were recruited through a self-administered questionnaire distributed via social media to humanitarian groups. Additionally, the questionnaire was sent via email to these groups' members, who then forwarded it to their respective networks. The questionnaire included the BRCS, a 4-item measure designed to capture tendencies to cope with stress in a highly adaptive manner. A score of 4-13 points indicates low resilient copers, 14-16 points medium resilient copers and 17-20 points high resilient copers. Cronbach's alpha was used to assess internal consistency. Confirmatory Factor Analysis (CFA) was employed to evaluate model fit. Adequate or good fit criteria included a χ2 test p-value ≥ 0.05, Root Mean Square Error Approximation (RMSEA) ≤ 0.08, Standardized Root Mean Squared Residual (SRMR) ≤ 0.05, and a Comparative Fit Index (CFI) or Tucker–Lewis Index (TLI) ≤ 0.90. Statistical analyses were performed using STATA and SPSS software. Results: A total of 151 humanitarian workers (76% females), with a mean age of 39.3 ±10.6 years participated in the study. The mean BRCS score was 65.6/100. Participants were categorized as follows: 34.6% as low resilient copers, 38.6% as medium resilient copers, and 26.8% as high resilient copers. Cronbach's alpha for the BRCS was 0.84, indicating good internal consistency. CFA results supported the one-factor solution proposed by the original researchers, with acceptable global fit indices: Chi-square p-value = 0.303, SRMR = 0.028, RMSEA = 0.036, CFI = 0.991, TLI = 0.974. Conclusions: The findings of our study show that the Greek version of BRCS is a valid and reliable tool that can be used to evaluate resilient coping among humanitarian workers in Greece. Disclosure of Interest: None Declared [ABSTRACT FROM AUTHOR]

Background: Aging of population brings changes in needs and capacities. People live longer and chronic diseases appear to be a potential risk of major causes of disability and functional dependency. The aim of this study was to investigate the physical and mental health of the elderly residing in an urban area in Athens,Greece. Methods: In this population-based study using a convenience sampling, seniors aged ≥ 65 years old, under no medical treatment were selected from Korydalos area, Athens, Greece. A translated version of the General Health Questionnaire (GHQ-28) was used and associations with other parameters were performed. Higher GHQ-28 scores indicate higher levels of distress. Results: A total of 100 seniors participated in the study, 64% females, mean age 71.4 years. 51% were married, 40% lived alone and 82% had primary or secondary education. 59% received a pension from the state, out of them 42% between 601-1000 euros and 33% between 301-600 euros, monthly. The mean GHQ-28 score, for a potential mental disorder in the somatization, anxiety, social dysfunction, and depression factors, was 24.3, 29.1, 42.9 and 10.2, respectively. Females presented higher scores in total GHQ score (p = 0.018), in somatization (p = 0.019) and in social dysfunction (p = 0.001). Higher age was associated with higher total GHQ score (p = 0.001), higher anxiety (p = 0,006) and higher dysfunction (p = 0.006). Seniors with lower educational level had higher total GHQ score (p = 0.032) and higher social dysfunction (p < 0.001). Low financial status was associated with higher social dysfunction (p = 0.005). Conclusions: The study revealed an overall moderate emotional distress in elderly. However, a high risk in social dysfunction is obvious, whereas risk of depression is very low. Age, educational level and financial status are associated with various dimensions of distress and psychiatric disorders. GHQ-28 is a suitable screening tool and can be used to prevent chronic disease and mental disorder. Key messages: • GHQ-28 can be used as a screening tool in elderly in urban communities to assess psychological well-being and detect possible psychiatric morbidity and to improve their overall health. [ABSTRACT FROM AUTHOR]

Background: Cervical cancer prevention can be obtained by regular screening with Papanicolaou test. However, in recent years HPV DNA test and mRNA HPV test are available for the screening for cervical cancer. The HPV vaccine helps protect against HPV- related cancers. Our aim was to investigate the beliefs and knowledge of women living in a city in northwestern Thessaly, Greece, regarding the prevention of cervical cancer. Methods: A cross sectional study was conducted among 119 women, 18- 65 years old and living in Trikala, Greece. They completed a 45-item self-administered questionnaire, investigating their knowledge, perceptions, and behaviors regarding the prevention of cervical cancer, the infection with HPV, the Papanicolaou test and vaccination. Furthermore, associations between knowledge and behaviors as well as with various demographic factors were explored. Results: Most of the women had at least university education (77%). More than 90% declared to visit an obstetrician-gynecologist at least once a year and 68.4% regularly have a Pap smear once a year. 95.8% were aware of the importance of a Pap smear and 82% declared a positive attitude regarding HPV vaccination. Higher knowledge regarding examination and vaccination, was associated with marital status (married) (p = 0.002) and educational level (higher educational level) (p = 0.001). Conclusions: The majority of women in our sample know why they should do the Pap test; however, they need to be further informed with additional details about the diagnostic test. Although most of the women were aware about the importance of testing, one third of them were not regularly tested for cervical cancer detection or had lacked knowledge about vaccination. Key messages: · A high percentage of women is aware about the benefits of cervical cancer screening. · However, information campaigns and establishment of national screening programs are required to facilitate the access to such programs for all women. [ABSTRACT FROM AUTHOR]

Background: Patient satisfaction is an important process for investigating patients’ needs, views, and suggestions about the health services provided by a health care facility. The aim of this study was to investigate women’s postnatal satisfaction with healthcare services during COVID-19 pandemic, provided at two regional public hospitals in Ioannina, Greece. Methods: Using a purposive and snowball sampling technique, a crosssectional study was performed among women who gave birth in two regional Greek hospitals, during October 2020. An online self-administered 32-item questionnaire was distributed personally, as well as, in social media groups related to young mothers and baby feeding. Results: A total of 106 women answered the questionnaire, mean age 34 years old, 58.5% had higher education and for 40.6% it was the first birth. 63.2% declared having visited the same hospital already in the past for gynecological services and 69.6% being satisfied with doctors’ and nurses’ communication, their interaction, behavior giving guidance, and the time they dedicated to the mothers. 60% were satisfied with hospital cleanliness, organization, adequacy, easy access, orientation and 63.6% from the administrative services. 23.8% were not satisfied with waiting times between appointment schedule and medical examination; however, 61.6% indicated that they would either use or suggest the services again. Higher satisfaction was associated with being married and employed, having no previous experience, and being in good health status. Conclusions: In general, a moderate satisfaction with various dimensions of provided health services was observed. The study highlights the importance of the postnatal care provided by healthcare professionals and health services. Improving the parameters which affect women’s satisfaction would increase the quality of postnatal care. Key messages: · Assessing patient satisfaction is an important indicator of measuring the quality of health services. [ABSTRACT FROM AUTHOR]

Introduction: Children with traumatic brain injury (TBI) are at risk for post-traumatic stress disorder (PTSD). The vast majority of TBI are of mild severity (MTBI), however, they may develop persistent neurophysiological symptoms. Objectives: The purpose of this study was to investigate the incidence of PTSD in children with MTBI in Western Greece. Methods: A one-year prospective study was conducted at the Children Hospital of Patras. A total of 175 children aged 6-14 years screened for risk of PTSD at one-week and one-month post-injury, completing the Child Trauma ScreeningQuestionnaire (CTSQ). The Children's Revised Impact of Event Scale (CRIES 13) was administered to the parents, to inquire their assessment of PTSD in the children. Statistical analysis was performed with IBM SPSS v.22.0 Results: There were 59 (33.7%) children (27.2% boys, 45.9% girls) whose screen result was at risk. At the rescreening one-month postinjury, 9.9% were still at risk. Parents assessed presence of PTSD in 19% of their children at one-week and in 3.9% at onemonth post-injury. There was a positive correlation between parenting and child reporting on symptoms of PTSD in children. However, 23.4% mistakenly estimated their children did not experience stress while in fact they did and 24.2% mistakenly estimated the contrary. Conclusions: The findings revealed the risk of PTSD even in mild TBI, justifying thus the screening to identify these children for intervention strategies. On the other hand, the rescreening demonstrated that not all at-risk children required intervention, since a natural remission in PTSD symptoms was observed one-month post-injury. [ABSTRACT FROM AUTHOR]

Introduction: The coronavirus pandemic has challenged the world with an unprecedented situation. Social distancing, self or quarantine isolation, personal hand hygiene, self-protection, and the fear of becoming infected with the virus, come with a psychological fallout. The COVID-19 pandemic has affected students around the world, in terms of their education and lifestyle. Objectives: To investigate the impact of COVID-19 pandemic on the students' mental health and well-being at the University of Patras, in Western Greece. Methods: An online questionnaire was prepared to collect responses from students during April 2020. Socio-demographic data, academic status, opinions about distance learning, changes in daily routine during the lockdown and anxiety and depression scores, according to the Greek version of the Hospital Anxiety and Depression Scale (HADS), were gathered. Results: The total number of responders was 2009, of which 67.3% women. During lockdown, the 68% of the students returned to their family home. Anxiety and depression scores were higher in students with a low income, poor self-rated health, not informed about COVID-19, not satisfied with distance learning and being annoyed at staying home. Prevalence of anxiety and depression was found to be 35.8% and 51.2%, ranging from 26.7% to 48.2% for anxiety and from 36.3% to 60.5% for depression in Health Sciences and Humanities and Social Sciences, respectively. Conclusions: Depression rates among university students in Greece were alarmingly high, denoting the impact of lockdown and changes in students' life, due to the COVID-19 pandemic. [ABSTRACT FROM AUTHOR]

Introduction: Radiation treatment plays a vital role in curative and palliative cancer therapy. In elderly cancer patients (ECPs) who may have compromised organ function and/or co-morbidities, the measurement of quality of life (QoL) is increasingly being recognized as an important patient-reported outcome to determine the burden of cancer treatment in this population. Objectives: To investigate the effect of radiotherapy on QoL, functional outcomes of ECPs and identify the risk factors for low QoL. Methods: Cross sectional study was performed on consecutively recruited patients from the Department of Radiation Oncology. The EORTC QLQ-C30 was administered to patients ≥65 years undergoing radiotherapy. It consists of 30 single questions, comprising five functioning scales (physical, role, cognitive, emotional, social), nine symptom items and a global quality of life scale. Results: A total of 48 patients answered the questionnaire, 65.1% males, mean age 73 years. Global health status/QoL score was 79.7%. Regarding the functional scales, lower scores were observed in physical (56%), social (58.3%), emotional (60.7%), and role functioning (61.5%), whereas cognitive functioning (71.5%) revealed higher score. Females had higher score in social functioning (63.2% vs 55.6%, p<0.05) and patients ≥75 years old scored in all functional scales lower compared to 65-75 years old patients (p<0.05). Conclusions: All functioning scales of EORTC QLQ-C30 had scores above 50, suggesting thus, that radiotherapy may not have detrimental effects on QoL in most ECPs with solid tumors. However, measuring QoL in this population group is important for clinical decision-making and the evaluation of treatment outcomes. [ABSTRACT FROM AUTHOR]

Introduction: Involuntary admissions (IA) affect the patients' autonomy and take place in order to prevent them to harm themselves or other people. The incidence of compulsory assessment (CA) seems to increase worldwide. Objectives: To investigate the epidemiological patterns of patients hospitalized involuntary after CA, during the economic crisis in North-West Greece. Methods: During 2009-2017, CA and IA were retrospectively assessed from the records of patients admitted to the Psychiatry Department at the University Hospital of Ioannina. Sociodemographic characteristics and data regarding legal procedures were collected. Results: A total of 602 CA were identified, 50% of them (every second year) were evaluated, 284 (85.5%) leaded to IA. The majority were men (67.5%), mean age 49 years, unmarried (70.2%), living with parents or siblings (74.8%), not working (64.8%), without tertiary education (70.2%), with residence in Ioannina (51.5%). First diagnosis was performed by a public hospital psychiatrist (88.6%), average length of stay was 24 days and most of them had been hospitalized in the past (64.2%). In 2009 there were 47 IA, whereas in 2017 there were 83. During 2009, the Femaleto-Male ratio for IA was 1 to 2.4, whereas in 2017 we observed 1 to 1.3, respectively. The main reason for IA was schizophrenia (56%) and to a lesser extent mood disorder (19.6%). Conclusions: The economic crisis seems to affect IA. Between 2009 and 2017 there was an increase in IA and an increase in women IA. [ABSTRACT FROM AUTHOR]

Inflammatory bowel diseases (IBD), including Crohn's disease (CD) and ulcerative colitis (UC) are chronic immune-mediated diseases which primarily target the intestines. In recent years, the development and regulatory approval of various immunotherapies, both biological agents and small molecules, that target specific pathways of the IBD-associated inflammatory cascade have revolutionized the treatment of IBD. Small molecules offer the advantages of oral administration and short wash-out times. Sphingosine-1-phosphate (S1P) is a bioactive metabolite of ceramide, which exerts its functions after binding to five G-protein-coupled receptors (S1PR1-S1PR5). Concerning IBD, S1P participates in the egress of lymphocytes from the secondary lymphoid tissue and their re-circulation to sites of inflammation, mainly through S1PR1 binding. In addition, this system facilitates the differentiation of T-helper cells towards proinflammatory immunophenotypes. Recently, S1P modulators have offered a valuable addition to the IBD treatment armamentarium. They exert their anti-inflammatory function via sequestration of T cell subsets in the lymphoid tissues and prevention of gut homing. In this review, we revisit the role of the S1P/S1PR axis in the pathogenesis of IBD and discuss efficacy and safety data from clinical trials and real-world reports on the two S1PR modulators, ozanimod and etrasimod, that are currently approved for IBD treatment, and comment on their potential positioning in the IBD day-to-day management. We also present recent data on emerging S1P modulators. Finally, based on the successes and failures of S1PR modulators in IBD, we discuss future avenues of IBD treatments targeting the S1P/S1PR axis., (© 2024. The Author(s), under exclusive licence to Springer Nature Switzerland AG.)

Anamnestic 13-valent pneumococcal conjugate vaccine immunization did not affect the relapse risk in pediatric idiopathic nephrotic syndrome. Pneumococcal serotype (PS)-specific antibody titers increased significantly in all groups. Children receiving immunomodulatory treatments (IMTs) displayed significantly lower levels of PS-specific antibodies for 3/8 serotypes tested. PS-specific B-cell counts significantly increased only in healthy controls and patients receiving corticosteroids., (© The Author(s) 2024. Published by Oxford University Press on behalf of The Journal of the Pediatric Infectious Diseases Society.)

Human endogenous retroviruses (HERVs), the remnants of ancient germline retroviral integrations, comprise almost 8% of the human genome. The elucidation of their biological roles is hampered by our inability to link HERV mRNA and protein production with specific HERV loci. To solve the riddle of the integration-specific RNA expression of HERVs, several bioinformatics approaches have been proposed; however, no single process seems to yield optimal results due to the repetitiveness of HERV integrations. The performance of existing data-bioinformatics pipelines has been evaluated against real world datasets whose true expression profile is unknown, thus the accuracy of widely-used approaches remains unclear. Here, we simulated mRNA production from specific HERV integrations to evaluate second and third generation sequencing technologies along with widely used bioinformatic approaches to estimate the accuracy in describing integration-specific expression. We demonstrate that, while a HERV-family approach offers accurate results, per-integration analyses of HERV expression suffer from substantial expression bias, which is only partially mitigated by algorithms developed for calculating the per-integration HERV expression, and is more pronounced in recent integrations. Hence, this bias could erroneously result into biologically meaningful inferences. Finally, we demonstrate the merits of accurate long-read high-throughput sequencing technologies in the resolution of per-locus HERV expression., (© The Author(s) 2024. Published by Oxford University Press on behalf of NAR Genomics and Bioinformatics.)

Human endogenous retroviruses (HERVs) are the result of retroviral infections acquired millions of years ago; nowadays, they compose around 8% of human DNA. Multiple mechanisms have been employed for endogenous retroviral deactivation, rendering replication and retrotransposition defective, while some of them have been co-opted to serve host evolutionary advantages. A pleiad of mechanisms retains the delicate balance of HERV expression in modern humans. Thus, epigenetic modifications, such as DNA and histone methylation, acetylation, deamination, chromatin remodeling, and even post-transcriptional control are recruited. In this review, we aim to summarize the main HERV silencing pathways, revisit paradigms of human disease with a HERV component, and emphasize the human immunodeficiency virus (HIV) and HERV interactions during HIV infection.

Importance: In this work, we demonstrated that human immunodeficiency virus (HIV) infection leads to the modification of the human endogenous retrovirus (HERV) expression. Differential expression of multiple HERVs was found in peripheral blood mononuclear cells derived from HIV-infected patients compared to healthy donors and HIV-infected T cell cultures compared to non-infected. The effect of HIV presence on HERV expression appears to be more restricted in cells of monocytic origin, as only deregulation of HERV-W and HERV-K (HML-6) was found in these cell cultures after their infection with HIV. Multiple factors contribute to this aberrant HERV expression, and its levels appear to be modified in a time-dependent manner. Further studies and the development of optimized in vitro protocols are warranted to elucidate the interactions between HIV and HERVs in detail., Competing Interests: The authors declare no conflict of interest.

Eighty percent of antibody secreting cells (ASCs) are found in the intestine, where they produce grams of immunoglobulin (Ig) A daily. immunoglobulin A is actively transcytosed into the lumen, where it plays a critical role in modulating the gut microbiota. Although loss of immune tolerance to bacterial antigens is the likely trigger of the dysregulated immune response that characterizes inflammatory bowel disease (IBD), little effort has been placed on understanding the interface between B cells, IgA, and the microbiota during initiation or progression of disease. This may be in part due to the misleading fact that IgA-deficient humans are mostly asymptomatic, likely due to redundant role of secretory (S) IgM. Intestinal B cell recruitment is critically dependent on integrin α4β7-MAdCAM-1 interactions, yet antibodies that target α4β7 (ie, vedolizumab), MAdCAM-1 (ie, ontamalimab), or both β7 integrins (α4β7 and αE [CD103] β7; etrolizumab) are in clinical use or development as IBD therapeutics. The effect of such interventions on the biology of IgA is largely unknown, yet a single dose of vedolizumab lowers SIgA levels in stool and weakens the oral immunization response to cholera vaccine in healthy volunteers. Thus, it is critical to further understand the role of these integrins for the migration of ASC and other cellular subsets during homeostasis and IBD-associated inflammation and the mode of action of drugs that interfere with this traffic. We have recently identified a subset of mature ASC that employs integrin αEβ7 to dock with intestinal epithelial cells, predominantly in the pericryptal region of the terminal ileum. This role for the integrin had not been appreciated previously, nor the αEβ7-dependent mechanism of IgA transcytosis that it supports. Furthermore, we find that B cells more than T cells are critically dependent on α4β7-MAdCAM-1 interactions; thus MAdCAM-1 blockade and integrin-β7 deficiency counterintuitively hasten colitis in interleukin-10-deficient mice. In both cases, de novo recruitment of IgA ASC to the intestinal lamina propria is compromised, leading to bacterial overgrowth, dysbiosis, and lethal colitis. Thus, despite the safe and effective use of anti-integrin antibodies in patients with IBD, much remains to be learned about their various cell targets., (© The Author(s) 2023. Published by Oxford University Press on behalf of Crohn’s & Colitis Foundation. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)

Human Endogenous Retroviruses (HERVs) are viral sequences integrated into the human genome, resulting from the infection of human germ-line cells by ancient exogenous retroviruses. Despite losing their replication and retrotransposition abilities, HERVs appear to have been co-opted in human physiological functions while their aberrant expression is linked to human disease. The role of HERVs in multiple malignancies has been demonstrated, however, the extent to which HERV activation and expression participate in the development of cancer is not yet fully comprehended. In this review article, we discuss the presumed role of HERVs in carcinogenesis and their promising diagnostic and prognostic implications. Additionally, we explore recent data on the HERVs in cancer therapeutics, either through the manipulation of their expression, to induce antitumor innate immunity responses or as cancer immunotherapy targets. Finally, more precise and higher resolution high-throughput sequencing approaches will further elucidate HERV participation in human physiological and pathological processes., (© 2022 The Authors. Journal of Medical Virology published by Wiley Periodicals LLC.)

Idiopathic nephrotic syndrome (INS) is a common glomerular disease in childhood, and the immunological involvement in the pathogenesis of non-genetic INS, although not fully elucidated, is evident. This narrative review aims to offer a concise and in-depth view of the current knowledge on the immunological mechanisms of the development of INS as well as the role of the immunological components of the disease in the responsiveness to treatment. T cell immunity appears to play a major role in the INS immunopathogenesis and has been the first to be linked to the disease. Various T cell immunophenotypes are implicated in INS, including T-helper-1, T-helper-2, T-helper-17, and T regulatory cells, and various cytokines have been proposed as surrogate biomarkers of the disease; however, no distinct T helper or cytokine profile has been conclusively linked to the disease. More recently, the recognition of the role of B cell mediated immunity and the various B cell subsets that are dysregulated in patients with INS have led to new hypotheses on the underlying immunological causes of INS. Finally, the disambiguation of the exact mechanisms of the INS development in the future may be the key to the development of more targeted personalized approaches in managing INS., Conclusions: INS demonstrates particularly interesting immunopathogenetic pathways, in which multiple interactions between T cell and B cell immunity and the podocyte are involved. The disambiguation of these pathways will provide promising novel therapeutic targets in INS., What Is Known: • INS is the most common glomerular disease in the paediatric population, and its onset and relapses have been linked to various immunological triggers. • Multiple immunological mechanisms have been implicated in the pathogenesis of INS; however, no single distinct immunological profile has been recognized., What Is New: • Th17 cells and Treg cells play an important role in the immune dysregulation in INS. • Transitional B cell levels as well as the transitional/memory B cell ratio have been correlated to nephrotic relapses and have been proposed as biomarkers of INS relapses in SSNS patients., (© 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

Severe COVID-19 pneumonia has been associated with the development of intense inflammatory responses during the course of infections with SARS-CoV-2. Given that human endogenous retroviruses (HERVs) are known to be activated during and participate in inflammatory processes, we examined whether HERV dysregulation signatures are present in COVID-19 patients. By comparing transcriptomes of bronchoalveolar lavage fluid (BALF) of COVID-19 patients and healthy controls, and peripheral blood monocytes (PBMCs) from patients and controls, we have shown that HERVs are intensely dysregulated in BALF of COVID-19 patients compared to those in BALF of healthy control patients but not in PBMCs. In particular, upregulation in the expression of specific HERV families was detected in BALF samples of COVID-19 patients, with HERV-FRD being the most highly upregulated family among the families analyzed. In addition, we compared the expression of HERVs in human bronchial epithelial cells (HBECs) without and after senescence induction in an oncogene-induced senescence model in order to quantitatively measure changes in the expression of HERVs in bronchial cells during the process of cellular senescence. This apparent difference of HERV dysregulation between PBMCs and BALF warrants further studies in the involvement of HERVs in inflammatory pathogenetic mechanisms as well as exploration of HERVs as potential biomarkers for disease progression. Furthermore, the increase in the expression of HERVs in senescent HBECs in comparison to that in noninduced HBECs provides a potential link for increased COVID-19 severity and mortality in aged populations. IMPORTANCE SARS-CoV-2 emerged in late 2019 in China, causing a global pandemic. Severe COVID-19 is characterized by intensive inflammatory responses, and older age is an important risk factor for unfavorable outcomes. HERVs are remnants of ancient infections whose expression is upregulated in multiple conditions, including cancer and inflammation, and their expression is increased with increasing age. The significance of this work is that we were able to recognize dysregulated expression of endogenous retroviral elements in BALF samples but not in PBMCs of COVID-19 patients. At the same time, we were able to identify upregulated expression of multiple HERV families in senescence-induced HBECs in comparison to that in noninduced HBECs, a fact that could possibly explain the differences in disease severity among age groups. These results indicate that HERV expression might play a pathophysiological role in local inflammatory pathways in lungs afflicted by SARS-CoV-2 and their expression could be a potential therapeutic target.

Being responsible for almost 12% of cancers worldwide, viruses are among the oldest known and most prevalent oncogenic agents. The quality of the evidence for the in vivo tumorigenic potential of microorganisms varies, thus accordingly, viruses were classified in 4 evidence-based categories by the International Agency for Research on Cancer in 2009. Since then, our understanding of the role of viruses in cancer has significantly improved, firstly due to the emergence of high throughput sequencing technologies that allowed the "brute-force" recovery of unknown viral genomes. At the same time, multi-omics approaches unravelled novel virus-host interactions in stem-cell biology. We now know that viral elements, either exogenous or endogenous, have multiple sometimes conflicting roles in human pathophysiology and the development of cancer. Here we integrate emerging evidence on viral causality in human cancer from basic mechanisms to clinical studies. We analyze viral tumorigenesis under the scope of deep-in-time human-virus evolutionary relationships and critically comment on the evidence through the eyes of clinical epidemiology, firstly by reviewing recognized oncoviruses and their mechanisms of inducing tumorigenesis, and then by examining the potential role of integrated viruses in our genome in the process of carcinogenesis., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2021 Kitsou, Iliopoulou, Spoulou, Lagiou and Magiorkinis.)

The presence of pharmaceuticals in the aquatic environment has become a cause for increasing concern in recent years. A wide range of pharmaceuticals have been discovered in fresh and marine waters and it has recently been shown that even in small quantities these compounds have the potential to cause harm to aquatic life. Their main pathway into the environment is through household use, and the disposal of unused or expired pharmaceuticals as manufacture is well-regulated. This work aimed to investigate the link between risk perception and household disposal. A survey was carried out, and around 400 householders, predominantly from the South-East of England, were interviewed. Information on when and how they disposed of unfinished pharmaceuticals was gathered. These responses were cross referenced with data on personal information and attitudes in order to determine the factors affecting the methods of disposal that are chosen. Although a link between perception of risk and the choice of disposal methods was not proved, findings demonstrated that perceived environmental awareness had an impact on the method of disposal chosen.

Introduction: Spontaneous electromyographic (EMG) activity in the soleus muscle of the rat varies with the changing gravitational force in parabolic flight, presumably in an appropriate way to resist the load. We investigated how decreased and increased gravitational force affects EMG in human back and arm muscles and to what extent the motor cortex is responsible for any modulation seen., Methods: Three healthy subjects stood during 10 parabolas consisting of periods (duration 20-25 s) of 1.8 G, then 0 G, and then 1.8 G. EMG recordings were made from right deltoid and left and right erector spinae (ES) muscles and transcranial magnetic stimulation (TMS) was applied to the motor cortex to produce motor evoked potentials (MEPs) in target muscles., Results: In one exemplary subject, EMG levels and MEP areas increased in the left ES and right ES during periods of 0 G, which was less pronounced with the arm abducted. No significant changes were seen in EMG levels or MEP areas during periods of 1.8 G. Pooled data from the three subjects showed a similar pattern, revealing a facilitation of MEP responses in left and right ES muscles in periods of 0 G., Discussion: EMG levels and MEP areas in ES muscles increased during periods of 0 G, suggesting that back muscle activity is "turned on" to stabilize the axial skeleton when the vertical compression forces present on Earth are removed. Further analysis suggested that microgravity produced activation of ES muscles through an increase in corticospinal excitability.