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Abstract Single-molecule tracking provides direct real-time measurements of protein dynamics in live cells with high spatiotemporal resolution. In the last decade, this method has been adopted by several labs to understand the dynamics of DNA replication, transcription, telomerase, chromatin remodeling, etc. in a variety of model systems (bacteria, yeast S. cerevisiae, cell lines, embryo). However, it has not been employed for the yeast Schizosaccharomyces pombe, despite being a valuable model system. Here, we present single-molecule tracking datasets for chromatin-bound histone H3 (Hht1) in live and fixed cells and freely diffusing GFP in S. pombe nuclei to benchmark the diffusion parameters (diffusion coefficient, mean squared displacement, fraction of bound molecules, residence time). These parameters will be used to differentiate chromatin-bound molecules from unbound (free) molecules of any protein. It will be a valuable resource for any lab that starts employing this method for studying protein dynamics. This dataset can also be used to validate the performance of various tracking software and as a training dataset for machine learning-based automated tracking.

Rivers are our country's lifeline; however, we have done enough destruction to them which leads to deterioration in water quality. Fortunately, COVID-19 lockdown has brought new life to nature. This encouraged us to outline present review article which discusses pilot impacts of lockdown on six Indian rivers. Few rivers including Ganga showed major improvement at few sites in the assessed parameters such as pH, BOD, DO, FC, etc. The Ganga water at Haridwar and Rishikesh was investigated 'fit for drinking' (Class A) while at Kanpur was found fit for 'outdoor bathing' (Class B). These improvements can be attributed to strict restriction on human activities during lockdown as there were no or minimum industrial discharge, tourism activities, mass bathing and commercial events near rivers. However, after upliftment of lockdown, these activities will return to their previous state and most likely pollutants will eventually reappear in the water bodies. So, in this review we have reviewed government's existing water pollution control schemes, analysed their limitations and recommended several scopes for improvement. Further research directions in this area have also been highlighted. We believe that plans and actions described in the article, if implemented, will lead to fruitful outcomes in managing water resources. [ABSTRACT FROM AUTHOR]