Search

Your search keyword '"Kirschner, Marc W."' showing total 982 results
Start Over Author "Kirschner, Marc W."
982 results on '"Kirschner, Marc W."'

5. The dynamics of gene expression in vertebrate embryogenesis at single-cell resolution

Author

Briggs, James A, Weinreb, Caleb, Wagner, Daniel E, Megason, Sean, Peshkin, Leonid, Kirschner, Marc W, and Klein, Allon M

Subjects
Pediatric, Genetics, 1.1 Normal biological development and functioning, Underpinning research, Generic health relevance, Animals, Cell Differentiation, Embryonic Development, Gene Expression Profiling, Gene Expression Regulation, Developmental, Genetic Variation, Neural Crest, Neurogenesis, Pluripotent Stem Cells, Sequence Analysis, RNA, Single-Cell Analysis, Transcription Factors, Transcriptome, Xenopus, Zebrafish, Zygote, General Science & Technology
Abstract

Time series of single-cell transcriptome measurements can reveal dynamic features of cell differentiation pathways. From measurements of whole frog embryos spanning zygotic genome activation through early organogenesis, we derived a detailed catalog of cell states in vertebrate development and a map of differentiation across all lineages over time. The inferred map recapitulates most if not all developmental relationships and associates new regulators and marker genes with each cell state. We find that many embryonic cell states appear earlier than previously appreciated. We also assess conflicting models of neural crest development. Incorporating a matched time series of zebrafish development from a companion paper, we reveal conserved and divergent features of vertebrate early developmental gene expression programs.

Published
2018

8. Kinesin superfamily protein Kif26b links Wnt5a-Ror signaling to the control of cell and tissue behaviors in vertebrates.

Author

Susman, Michael W, Karuna, Edith P, Kunz, Ryan C, Gujral, Taranjit S, Cantú, Andrea V, Choi, Shannon S, Jong, Brigette Y, Okada, Kyoko, Scales, Michael K, Hum, Jennie, Hu, Linda S, Kirschner, Marc W, Nishinakamura, Ryuichi, Yamada, Soichiro, Laird, Diana J, Jao, Li-En, Gygi, Steven P, Greenberg, Michael E, and Ho, Hsin-Yi Henry

Subjects
Cell Line, Animals, Mice, Inbred C57BL, Humans, Mice, Kinesin, Proteomics, Signal Transduction, Gene Expression Regulation, Developmental, Morphogenesis, Embryonic Development, beta Catenin, Receptor Tyrosine Kinase-like Orphan Receptors, HEK293 Cells, Wnt Signaling Pathway, Wnt-5a Protein, Kif26b, Ror, developmental biology, mouse, noncanonical Wnt signaling, regulated proteolysis, signal transduction, stem cells, tissue morphogenesis, Regenerative Medicine, Stem Cell Research, 1.1 Normal biological development and functioning, Biochemistry and Cell Biology
Abstract

Wnt5a-Ror signaling constitutes a developmental pathway crucial for embryonic tissue morphogenesis, reproduction and adult tissue regeneration, yet the molecular mechanisms by which the Wnt5a-Ror pathway mediates these processes are largely unknown. Using a proteomic screen, we identify the kinesin superfamily protein Kif26b as a downstream target of the Wnt5a-Ror pathway. Wnt5a-Ror, through a process independent of the canonical Wnt/β-catenin-dependent pathway, regulates the cellular stability of Kif26b by inducing its degradation via the ubiquitin-proteasome system. Through this mechanism, Kif26b modulates the migratory behavior of cultured mesenchymal cells in a Wnt5a-dependent manner. Genetic perturbation of Kif26b function in vivo caused embryonic axis malformations and depletion of primordial germ cells in the developing gonad, two phenotypes characteristic of disrupted Wnt5a-Ror signaling. These findings indicate that Kif26b links Wnt5a-Ror signaling to the control of morphogenetic cell and tissue behaviors in vertebrates and reveal a new role for regulated proteolysis in noncanonical Wnt5a-Ror signal transduction.

Published
2017

9. Noncanonical open reading frames encode functional proteins essential for cancer cell survival

Author

Prensner, John R., Enache, Oana M., Luria, Victor, Krug, Karsten, Clauser, Karl R., Dempster, Joshua M., Karger, Amir, Wang, Li, Stumbraite, Karolina, Wang, Vickie M., Botta, Ginevra, Lyons, Nicholas J., Goodale, Amy, Kalani, Zohra, Fritchman, Briana, Brown, Adam, Alan, Douglas, Green, Thomas, Yang, Xiaoping, Jaffe, Jacob D., Roth, Jennifer A., Piccioni, Federica, Kirschner, Marc W., Ji, Zhe, Root, David E., and Golub, Todd R.

Published
2021
Full Text
View/download PDF

11. Preprints for the life sciences

Author

Berg, Jeremy M, Bhalla, Needhi, Bourne, Philip E, Chalfie, Martin, Drubin, David G, Fraser, James S, Greider, Carol W, Hendricks, Michael, Jones, Chonnettia, Kiley, Robert, King, Susan, Kirschner, Marc W, Krumholz, Harlan M, Lehmann, Ruth, Leptin, Maria, Pulverer, Bernd, Rosenzweig, Brooke, Spiro, John E, Stebbins, Michael, Strasser, Carly, Swaminathan, Sowmya, Turner, Paul, Vale, Ronald D, VijayRaghavan, K, and Wolberger, Cynthia

Subjects
Philosophy and Religious Studies, History and Philosophy Of Specific Fields, Biological Science Disciplines, Information Dissemination, Publishing, General Science & Technology
Abstract

The time is right for biologists to post their research findings onto preprint servers

Published
2016

12. SCIENTIFIC COMMUNITY. Preprints for the life sciences.

Author

Berg, Jeremy M, Bhalla, Needhi, Bourne, Philip E, Chalfie, Martin, Drubin, David G, Fraser, James S, Greider, Carol W, Hendricks, Michael, Jones, Chonnettia, Kiley, Robert, King, Susan, Kirschner, Marc W, Krumholz, Harlan M, Lehmann, Ruth, Leptin, Maria, Pulverer, Bernd, Rosenzweig, Brooke, Spiro, John E, Stebbins, Michael, Strasser, Carly, Swaminathan, Sowmya, Turner, Paul, Vale, Ronald D, VijayRaghavan, K, and Wolberger, Cynthia

Subjects
Information Dissemination, Publishing, Biological Science Disciplines, General Science & Technology
Published
2016

13. Hemichordate genomes and deuterostome origins.

Author

Simakov, Oleg, Kawashima, Takeshi, Marlétaz, Ferdinand, Jenkins, Jerry, Koyanagi, Ryo, Mitros, Therese, Hisata, Kanako, Bredeson, Jessen, Shoguchi, Eiichi, Gyoja, Fuki, Yue, Jia-Xing, Chen, Yi-Chih, Freeman, Robert M, Sasaki, Akane, Hikosaka-Katayama, Tomoe, Sato, Atsuko, Fujie, Manabu, Baughman, Kenneth W, Levine, Judith, Gonzalez, Paul, Cameron, Christopher, Fritzenwanker, Jens H, Pani, Ariel M, Goto, Hiroki, Kanda, Miyuki, Arakaki, Nana, Yamasaki, Shinichi, Qu, Jiaxin, Cree, Andrew, Ding, Yan, Dinh, Huyen H, Dugan, Shannon, Holder, Michael, Jhangiani, Shalini N, Kovar, Christie L, Lee, Sandra L, Lewis, Lora R, Morton, Donna, Nazareth, Lynne V, Okwuonu, Geoffrey, Santibanez, Jireh, Chen, Rui, Richards, Stephen, Muzny, Donna M, Gillis, Andrew, Peshkin, Leonid, Wu, Michael, Humphreys, Tom, Su, Yi-Hsien, Putnam, Nicholas H, Schmutz, Jeremy, Fujiyama, Asao, Yu, Jr-Kai, Tagawa, Kunifumi, Worley, Kim C, Gibbs, Richard A, Kirschner, Marc W, Lowe, Christopher J, Satoh, Noriyuki, Rokhsar, Daniel S, and Gerhart, John

Subjects
Animals, Chordata, Nonvertebrate, Echinodermata, Transforming Growth Factor beta, Evolution, Molecular, Phylogeny, Signal Transduction, Conserved Sequence, Synteny, Multigene Family, Genome, Chordata, Nonvertebrate, Evolution, Molecular, General Science & Technology
Abstract

Acorn worms, also known as enteropneust (literally, 'gut-breathing') hemichordates, are marine invertebrates that share features with echinoderms and chordates. Together, these three phyla comprise the deuterostomes. Here we report the draft genome sequences of two acorn worms, Saccoglossus kowalevskii and Ptychodera flava. By comparing them with diverse bilaterian genomes, we identify shared traits that were probably inherited from the last common deuterostome ancestor, and then explore evolutionary trajectories leading from this ancestor to hemichordates, echinoderms and chordates. The hemichordate genomes exhibit extensive conserved synteny with amphioxus and other bilaterians, and deeply conserved non-coding sequences that are candidates for conserved gene-regulatory elements. Notably, hemichordates possess a deuterostome-specific genomic cluster of four ordered transcription factor genes, the expression of which is associated with the development of pharyngeal 'gill' slits, the foremost morphological innovation of early deuterostomes, and is probably central to their filter-feeding lifestyle. Comparative analysis reveals numerous deuterostome-specific gene novelties, including genes found in deuterostomes and marine microbes, but not other animals. The putative functions of these genes can be linked to physiological, metabolic and developmental specializations of the filter-feeding ancestor.

Published
2015

15. Rescuing US biomedical research from its systemic flaws

Author

Alberts, Bruce, Kirschner, Marc W, Tilghman, Shirley, and Varmus, Harold

Subjects
Biomedical Research, Health Planning Guidelines, Humans, Research Personnel, Research Support as Topic, United States, Workforce, graduate education, postdoctoral education, federal funding, peer review
Abstract

The long-held but erroneous assumption of never-ending rapid growth in biomedical science has created an unsustainable hypercompetitive system that is discouraging even the most outstanding prospective students from entering our profession--and making it difficult for seasoned investigators to produce their best work. This is a recipe for long-term decline, and the problems cannot be solved with simplistic approaches. Instead, it is time to confront the dangers at hand and rethink some fundamental features of the US biomedical research ecosystem.

Published
2014

16. Membrane potential mediates an ancient mechano-transduction mechanism for multi-cellular homeostasis

Author

Mukherjee, Avik, primary, Huang, Yanqing, additional, Elgeti, Jens, additional, Oh, Seungeun, additional, Neliat, Anjali Rebecca, additional, Schüttler, Janik, additional, Benites, Nina Catherine, additional, Liu, Xili, additional, Barboiu, Mihail, additional, Stocker, Hugo, additional, Kirschner, Marc W., additional, and Basan, Markus, additional

Published
2023
Full Text
View/download PDF

17. VHL substrate transcription factor ZHX2 as an oncogenic driver in clear cell renal cell carcinoma

Author

Zhang, Jing, Wu, Tao, Simon, Jeremy, Takada, Mamoru, Saito, Ryoichi, Fan, Cheng, Liu, Xian-De, Jonasch, Eric, Xie, Ling, Chen, Xian, Yao, Xiaosai, Teh, Bin Tean, Tan, Patrick, Zheng, Xingnan, Li, Mingjie, Lawrence, Cortney, Fan, Jie, Geng, Jiang, Liu, Xjuan, Hu, Lianxin, Wang, Jun, Liao, Chengheng, Hong, Kai, Zurlo, Giada, Parker, Joel S., Auman, J. Todd, Perou, Charles M., Rathmell, W. Kimryn, Kim, William Y., Kirschner, Marc W., Kaelin, William G., Baldwin, Albert S., and Zhang, Qing

Published
2018

22. Using buoyant mass to measure the growth of single cells

Author

Godin, Michel, Delgado, Francisco Feijó, Son, Sungmin, Grover, William H, Bryan, Andrea K, Tzur, Amit, Jorgensen, Paul, Payer, Kris, Grossman, Alan D, Kirschner, Marc W, and Manalis, Scott R

Subjects
Bioengineering, Generic health relevance, Animals, Bacillus subtilis, Cell Cycle, Cell Enlargement, Escherichia coli, Lymphocytes, Mice, Microfluidic Analytical Techniques, Saccharomyces cerevisiae, Biological Sciences, Technology, Medical and Health Sciences, Developmental Biology
Abstract

We used a suspended microchannel resonator (SMR) combined with picoliter-scale microfluidic control to measure buoyant mass and determine the 'instantaneous' growth rates of individual cells. The SMR measures mass with femtogram precision, allowing rapid determination of the growth rate in a fraction of a complete cell cycle. We found that for individual cells of Bacillus subtilis, Escherichia coli, Saccharomyces cerevisiae and mouse lymphoblasts, heavier cells grew faster than lighter cells.

Published
2010

28. A universal mechanism of biomass density homeostasis via ribosomal counterions

Author

Mukherjee, Avik, primary, Huang, Yanqing, additional, Oh, Seungeun, additional, Sanchez, Carlos, additional, Chang, Yu-Fang, additional, Liu, Xili, additional, Bradshaw, Gary Andrew, additional, Benites, Nina Catherine, additional, Paulsson, Johan, additional, Kirschner, Marc W., additional, Sung, Yongjin, additional, Elgeti, Jens, additional, and Basan, Markus, additional

Published
2023
Full Text
View/download PDF

33. An actin-based wave generator organizes cell motility

Author

Weiner, Orion D, Marganski, William A, Wu, Lani F, Altschuler, Steven J, and Kirschner, Marc W

Abstract

Although many of the regulators of actin assembly are known, we do not understand how these components act together to organize cell shape and movement. To address this question, we analyzed the spatial dynamics of a key actin regulator-the Scar/WAVE complex-which plays an important role in regulating cell shape in both metazoans and plants. We have recently discovered that the Hem-1/Nap1 component of the Scar/WAVE complex localizes to propagating waves that appear to organize the leading edge of a motile immune cell, the human neutrophil. Actin is both an output and input to the Scar/WAVE complex: the complex stimulates actin assembly, and actin polymer is also required to remove the complex from the membrane. These reciprocal interactions appear to generate propagated waves of actin nucleation that exhibit many of the properties of morphogenesis in motile cells, such as the ability of cells to flow around barriers and the intricate spatial organization of protrusion at the leading edge. We propose that cell motility results from the collective behavior of multiple self-organizing waves.

Published
2007

40. Hem-1 Complexes Are Essential for Rac Activation, Actin Polymerization, and Myosin Regulation during Neutrophil Chemotaxis

Author

Weiner, Orion D, Rentel, Maike C, Ott, Alex, Brown, Glenn E, Jedrychowski, Mark, Yaffe, Michael B, Gygi, Steven P, Cantley, Lewis C, Bourne, Henry R, and Kirschner, Marc W

Subjects
Biochemistry and Cell Biology, Biological Sciences, 1.1 Normal biological development and functioning, Underpinning research, Generic health relevance, Actins, Animals, Cell Line, Cell Polarity, Chemotaxis, Enzyme Activation, Humans, Membrane Proteins, Molecular Sequence Data, Myosins, Neutrophils, Phosphatidylinositol Phosphates, Phosphorylation, Protein Binding, Protein Subunits, Reactive Oxygen Species, Terminology as Topic, Wiskott-Aldrich Syndrome Protein Family, rac GTP-Binding Proteins, Agricultural and Veterinary Sciences, Medical and Health Sciences, Developmental Biology, Agricultural, veterinary and food sciences, Biological sciences, Biomedical and clinical sciences
Abstract

Migrating cells need to make different actin assemblies at the cell's leading and trailing edges and to maintain physical separation of signals for these assemblies. This asymmetric control of activities represents one important form of cell polarity. There are significant gaps in our understanding of the components involved in generating and maintaining polarity during chemotaxis. Here we characterize a family of complexes (which we term leading edge complexes), scaffolded by hematopoietic protein 1 (Hem-1), that organize the neutrophil's leading edge. The Wiskott-Aldrich syndrome protein family Verprolin-homologous protein (WAVE)2 complex, which mediates activation of actin polymerization by Rac, is only one member of this family. A subset of these leading edge complexes are biochemically separable from the WAVE2 complex and contain a diverse set of potential polarity-regulating proteins. RNA interference-mediated knockdown of Hem-1-containing complexes in neutrophil-like cells: (a) dramatically impairs attractant-induced actin polymerization, polarity, and chemotaxis; (b) substantially weakens Rac activation and phosphatidylinositol-(3,4,5)-tris-phosphate production, disrupting the (phosphatidylinositol-(3,4,5)-tris-phosphate)/Rac/F-actin-mediated feedback circuit that organizes the leading edge; and (c) prevents exclusion of activated myosin from the leading edge, perhaps by misregulating leading edge complexes that contain inhibitors of the Rho-actomyosin pathway. Taken together, these observations show that versatile Hem-1-containing complexes coordinate diverse regulatory signals at the leading edge of polarized neutrophils, including but not confined to those involving WAVE2-dependent actin polymerization.

Published
2006

Catalog

Books, media, physical & digital resources
See catalog results