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2. Dermal Delivery of a SARS-CoV-2 Subunit Vaccine Induces Immunogenicity against Variants of Concern

Author

McMillan, Christopher L. D., primary, Azuar, Armira, additional, Choo, Jovin J. Y., additional, Modhiran, Naphak, additional, Amarilla, Alberto A., additional, Isaacs, Ariel, additional, Honeyman, Kate E., additional, Cheung, Stacey T. M., additional, Liang, Benjamin, additional, Wurm, Maria J., additional, Pino, Paco, additional, Kint, Joeri, additional, Fernando, Germain J. P., additional, Landsberg, Michael J., additional, Khromykh, Alexander A., additional, Hobson-Peters, Jody, additional, Watterson, Daniel, additional, Young, Paul R., additional, and Muller, David A., additional

Published
2022
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3. High-Titer Neutralizing Antibodies against the SARS-CoV-2 Delta Variant Induced by Alhydroxyquim-II-Adjuvanted Trimeric Spike Antigens

Author

Counoupas, Claudio, primary, Pino, Paco, additional, Stella, Alberto O., additional, Ashley, Caroline, additional, Lukeman, Hannah, additional, Bhattacharyya, Nayan D., additional, Tada, Takuya, additional, Anchisi, Stephanie, additional, Metayer, Charles, additional, Martinis, Jacopo, additional, Aggarwal, Anupriya, additional, Dcosta, Belinda M., additional, Britton, Warwick J., additional, Kint, Joeri, additional, Wurm, Maria J., additional, Landau, Nathaniel R., additional, Steain, Megan, additional, Turville, Stuart G., additional, Wurm, Florian M., additional, David, Sunil A., additional, and Triccas, James A., additional

Published
2022
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6. Neutralising antibodies against the SARS-CoV-2 Delta variant induced by Alhydroxyquim-II-adjuvanted trimeric spike antigens

Author

Counoupas, Claudio, primary, Pino, Paco, additional, Stella, Alberto O., additional, Ashley, Caroline, additional, Lukeman, Hannah, additional, Bhattacharyya, Nayan D., additional, Tada, Takuya, additional, Anchisi, Stephanie, additional, Metayer, Charles, additional, Martinis, Jacopo, additional, Aggarwal, Anupriya, additional, Dcosta, Belinda M., additional, Kint, Joeri, additional, Wurm, Maria J, additional, Landau, Nathaniel R., additional, Steain, Megan, additional, Turville, Stuart G, additional, Wurm, Florian M, additional, David, Sunil A., additional, and Triccas, James A., additional

Published
2021
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7. Site-Specific Steric Control of SARS-CoV-2 Spike Glycosylation

Author

Allen, Joel D., primary, Chawla, Himanshi, additional, Samsudin, Firdaus, additional, Zuzic, Lorena, additional, Shivgan, Aishwary Tukaram, additional, Watanabe, Yasunori, additional, He, Wan-ting, additional, Callaghan, Sean, additional, Song, Ge, additional, Yong, Peter, additional, Brouwer, Philip J. M., additional, Song, Yutong, additional, Cai, Yongfei, additional, Duyvesteyn, Helen M. E., additional, Malinauskas, Tomas, additional, Kint, Joeri, additional, Pino, Paco, additional, Wurm, Maria J., additional, Frank, Martin, additional, Chen, Bing, additional, Stuart, David I., additional, Sanders, Rogier W., additional, Andrabi, Raiees, additional, Burton, Dennis R., additional, Li, Sai, additional, Bond, Peter J., additional, and Crispin, Max, additional

Published
2021
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8. Trimeric SARS-CoV-2 Spike Proteins Produced from CHO Cells in Bioreactors Are High-Quality Antigens

Author

Pino, Paco, primary, Kint, Joeri, additional, Kiseljak, Divor, additional, Agnolon, Valentina, additional, Corradin, Giampietro, additional, Kajava, Andrey V., additional, Rovero, Paolo, additional, Dijkman, Ronald, additional, den Hartog, Gerco, additional, McLellan, Jason S., additional, Byrne, Patrick O., additional, Wurm, Maria J., additional, and Wurm, Florian M., additional

Published
2020
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9. Quantification of Interferon Signaling in Avian Cells

Author

Kint, Joeri and Forlenza, Maria

Subjects
Transcription, Genetic, Chicken, Quail, Article, Real-time quantitative PCR, Cell Line, Avian Proteins, Coronavirus, Host-Pathogen Interactions, Interferon Type I, Interferon, Animals, Bioassay, RNA, Messenger, Chickens, Signal Transduction
Abstract

Activation of the type I interferon (IFN) response is an essential defense mechanism against invading pathogens such as viruses. This chapter describes two protocols to quantify activation of the chicken IFN response through analysis of gene expression by real-time quantitative PCR and by quantification of bioactive IFN protein using a bioassay.

Published
2014

10. Infectious bronchitis coronavirus limits interferon production by inducing a host shutoff that requires accessory protein 5b

Author

Kint, Joeri, Langereis, Martijn A, Maier, Helena J, Britton, Paul, van Kuppeveld, Frank J, Koumans, Joseph, Wiegertjes, Geert F, Forlenza, Maria, Kint, Joeri, Langereis, Martijn A, Maier, Helena J, Britton, Paul, van Kuppeveld, Frank J, Koumans, Joseph, Wiegertjes, Geert F, and Forlenza, Maria

Abstract

During infection of their host cell, viruses often inhibit production of host proteins, a process which is referred to as host shutoff. By doing this, viruses limit production of antiviral proteins and increase production capacity for viral proteins. Coronaviruses from the Alpha- and Betacoronavirus genera, such as severe acute respiratory syndrome coronavirus (SARS-CoV) establish host shutoff via their non-structural protein 1 (nsp1). The genomes of Gamma- and Deltacoronaviruses however do not encode nsp1, and it has been suggested that these viruses do not induce host shutoff. Here we show that infectious bronchitis Gammacoronavirus (IBV) does induce host shutoff and we find that its accessory protein 5b is indispensable for this function. Importantly, we found that 5b-null viruses, unlike wild type viruses, induce production of high concentrations of type I interferon protein in vitro, indicating that host shutoff by IBV plays an important role in antagonizing the host's innate immune response. Altogether we demonstrate that 5b is a functional equivalent of nsp1 thereby answering the long-standing question whether lack of nsp1 in Gammacoronaviruses is compensated for by another viral protein. As such, our study is a significant step forward in the understanding of coronavirus biology and closes a gap in the understanding of some IBV virulence strategies.IMPORTANCE: Many viruses inhibit protein synthesis of their host cell to enhance virus replication and antagonize anti-viral defense mechanisms. This process is referred to as 'host-shutoff'. We have studied gene expression and protein synthesis in chicken cells infected with the important poultry pathogen, infectious bronchitis virus (IBV). We show that IBV inhibits synthesis of host proteins, including that of type I interferon, a key component of the antiviral response. The IBV-induced host shutoff however, does not require degradation of host RNA. Furthermore, we demonstrate that accessory protein 5b

Published
2016

11. Infectious bronchitis coronavirus limits interferon production by inducing a host shutoff that requires accessory protein 5b

Author

LS Virologie, dI&I I&I-1, Infection & Immunity, Kint, Joeri, Langereis, Martijn A, Maier, Helena J, Britton, Paul, van Kuppeveld, Frank J, Koumans, Joseph, Wiegertjes, Geert F, Forlenza, Maria, LS Virologie, dI&I I&I-1, Infection & Immunity, Kint, Joeri, Langereis, Martijn A, Maier, Helena J, Britton, Paul, van Kuppeveld, Frank J, Koumans, Joseph, Wiegertjes, Geert F, and Forlenza, Maria

Published
2016

15. Targeting Membrane-Bound Viral RNA Synthesis Reveals Potent Inhibition of Diverse Coronaviruses Including the Middle East Respiratory Syndrome Virus

Author

Lundin, Anna, primary, Dijkman, Ronald, additional, Bergström, Tomas, additional, Kann, Nina, additional, Adamiak, Beata, additional, Hannoun, Charles, additional, Kindler, Eveline, additional, Jónsdóttir, Hulda R., additional, Muth, Doreen, additional, Kint, Joeri, additional, Forlenza, Maria, additional, Müller, Marcel A., additional, Drosten, Christian, additional, Thiel, Volker, additional, and Trybala, Edward, additional

Published
2014
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