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Abstract Gene delivery to, and expression in, the mouse brain is important for understanding gene functions in brain development and disease, or testing gene therapies. Here, we describe an approach to express a transgene in the mouse brain in a cell-type-specific manner. We use stereotaxic injection of a transgene-expressing adeno-associated virus into the mouse brain via the intracerebroventricular route. We demonstrate stable and sustained expression of the transgene in neurons of adult mouse brain, using a reporter gene driven by a neuron-specific promoter. This approach represents a rapid, simple, and cost-effective method for global gene expression in the mouse brain, in a cell-type-specific manner, without major surgical interventions. The described method represents a helpful resource for genetically engineering mice to express a therapeutic gene, for gene therapy studies, or to deliver genetic material for genome editing and developing knockout animal models.

Abstract Cancer is a major public health problem as one of the leading causes of death worldwide. Deciphering the molecular regulation mechanisms of tumor progression can make way for tumor diagnosis and therapy. Paternally expressed gene 10 (PEG10), located on human chromosome 7q21.3, has turned out to be an oncogene implicated in the proliferation, apoptosis and metastasis of tumors. PEG10 has been found to be positively expressed in a variety of cancers with seemingly complex expression regulation mechanisms. In this review, we focus on the most vital factors influencing PEG10 expression and recapitulate some of the currently known and potential mechanisms of PEG10 affecting tumor progression, as understanding the molecular regulatory mechanisms of tumor progression can provide potential PEG10 related diagnosis and biomarker specific targeted therapies.

Abstract Cancer is currently one of the leading causes of death worldwide and is one of the most challenging major public health problems. The main challenges faced by clinicians in the management and treatment of cancer mainly arise from difficulties in early diagnosis and the emergence of tumor chemoresistance and metastasis. The structures of chemokine receptor 9 (CCR9) and its specific ligand chemokine ligand 25 (CCL25) have been elucidated, and, interestingly, a number of studies have demonstrated that CCR9 is a potential tumor biomarker in diagnosis and therapy, as it has been found to be highly expressed in a wide range of cancers. This expression pattern suggests that CCR9 may participate in many important biological activities involved in cancer progression. Researchers have shown that CCR9 that has been activated by its specific ligand CCL25 can interact with many signaling pathways, especially those involved in tumor chemoresistance and metastasis. This review, therefore, focuses on CCR9 induction activity and summarizes what is currently known regarding its role in cancers and its potential application in tumor-targeted therapy.

In this 14-day study, we tested whether Latinx adolescents' (Mage  = 12.76 years, 52% female; 52% U.S. born; N = 21) and parents' (95% female; 24% U.S. born) daily discrimination experiences were associated with their own and other's daily affective states. Results indicated that on days when adolescents reported discrimination, they reported higher negative affect and marginally lower positive affect and, interestingly, parents reported higher positive affect. On average (i.e., across the 2-week period), adolescents' discrimination was associated with higher adolescent negative affect and lower parent positive affect. Together, findings suggest that Latinx adolescents' discrimination experiences are linked to their own affective states and their parents'. Results underscore how discrimination is linked to the affective states present in family contexts.

The current study examined the trajectory of gender role attitudes of 471 Mexican-origin adolescents (236 girls, 235 boys) from 5th grade (Mage = 10.86 years) to 11th grade (Mage = 16.75 years), investigating how situating identities (i.e., gender, nativity, SES), ethnic identity (i.e., ethnic pride), and familial context (i.e., parents' attitudes) contributed to adolescents' gender role attitudes across time. Participant interviews were conducted every other year, resulting in 4 waves of data. Most parents (96%) were Mexico natives, with an average immigration age of 18.16 years for fathers and 14.01 years for mothers. Results revealed linear and quadratic trends in gender attitude traditionality for all adolescents, characterized by a linear decline through age 16 years that leveled off through age 18 years. Although both girls and boys trended toward egalitarian gender role attitudes across adolescence, girls endorsed more egalitarian attitudes than did boys. Adolescents from higher-SES backgrounds endorsed more egalitarian attitudes than those from lower-SES backgrounds. Significant within-person effects of ethnic pride surfaced, such that children with higher levels of ethnic pride at any given time also reported more traditional gender role attitudes. Significant between-person effects of mothers' and fathers' attitudes were found, such that parents with more traditional gender role attitudes tended to have children with relatively more traditional gender role attitudes. Overall, these findings highlight the importance of studying gender development in conjunction with situating identities, cultural identities, and the broader context, particularly when children are embedded in multiple cultures with contrasting gender role expectations. (PsycINFO Database Record (c) 2019 APA, all rights reserved).

This study assessed knowledge, attitudes, and practices related to food hygiene among mothers from Malawi’s rural communities against the WHO Five Keys to Safer Food (WHO-FKSF) and good pre-and post-harvest practices (GPPHPs) as reference points. Five hundred twenty-two mothers from six rural communities across two districts were selected for the survey. The results indicated limited knowledge among participating mothers regarding managing food hazards, including mycotoxins, bacteria, viruses, and parasites (BVPs). A significant proportion (89.5%) of women reported inconsistent or no handwashing with soap after using the toilet. In addition, 48.7% failed to plant with the first good rains, 38.7% neglected to check for moldy cobs during harvesting, 57.4% dried maize on bare soil, and 99.2% bought maize with noticeable mold. Higher education, knowledge, and positive attitudes were associated with enhanced BVP control practices, while larger households and positive attitudes were linked to improved mold/mycotoxin management (p < 0.05). Mothers showed lower (p < 0.05) knowledge and attitude levels regarding molds than BVPs but demonstrated relatively better practices for mold control. A comprehensive education program based on the WHO Five Keys to Safer Foods, tailored to local socio-cultural norms and incorporating mold and mycotoxin management guidelines, is recommended.