1. Natural plant hormones cytokinins increase stress resistance and longevity of Caenorhabditis elegans.
- Author
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Kadlecová A, Jirsa T, Novák O, Kammenga J, Strnad M, and Voller J
- Subjects
- Animals, Caenorhabditis elegans genetics, Caenorhabditis elegans physiology, Caenorhabditis elegans Proteins genetics, Caenorhabditis elegans Proteins metabolism, Cytokinins pharmacokinetics, Forkhead Transcription Factors genetics, Forkhead Transcription Factors metabolism, Heat-Shock Response drug effects, Insulin metabolism, Kinetin pharmacokinetics, Kinetin pharmacology, Longevity physiology, Mutation, Oxidative Stress drug effects, Plant Growth Regulators pharmacokinetics, Reactive Oxygen Species metabolism, Signal Transduction drug effects, Thermotolerance drug effects, Caenorhabditis elegans drug effects, Cytokinins pharmacology, Longevity drug effects, Plant Growth Regulators pharmacology
- Abstract
Cytokinins are phytohormones that are involved in many processes in plants, including growth, differentiation and leaf senescence. However, they also have various activities in animals. For example, kinetin and trans-zeatin can reduce levels of several aging markers in human fibroblasts. Kinetin can also protect mice against oxidative and glyoxidative stress, and prolong fruit flies' lifespan. Additionally, several cytokinins are currently used in cosmetics. To extend knowledge of the breadth of cytokinins' activities, we examined effects of natural cytokinin bases on the model nematode Caenorhabditis elegans. We found that kinetin, para-topolin and meta-topolin prolonged the lifespan of C. elegans. Kinetin also protected the organism against oxidative and heat stress. Furthermore, our results suggest that presence of reactive oxygen species, but not DAF-16 (the main effector of the insulin/insulin-like growth factor signaling pathway), is required for the beneficial effects of kinetin. Ultra-high performance liquid chromatography-tandem mass spectrometric analysis showed that kinetin is unlikely to occur naturally in C. elegans, but the worm efficiently absorbs and metabolizes it into kinetin riboside and kinetin riboside-5'-monophosphate.
- Published
- 2018
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