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Transitions between the Zeeman substates of Na(3p 2P1/2) and Na(3p 2P3/2) in collision with Na(3s 2S1/2) in thermal energy region are investigated by the close-coupling method. Rotational and spin–orbit couplings are taken into consideration, and potential energy functions generated by a quantum chemical calculation are employed. The cross sections strongly depend on the magnetic quantum numbers of the initial and final states. This dependence qualitatively coincides with an experimental observation in the presence of a weak magnetic field, although the magnetic field is not considered in the present calculation. Total cross section of the transition Na(2P3/2)→Na(2P1/2) is calculated to be 2.7×102 Å2 in good agreement with experiment. The following two mechanisms are found to dominantly contribute to the total cross section: (1) Transitions among the molecular states of Na2, 3Π2u↔3Π1u and 3Π1u↔3Π0u, induced by the spin–rotation coupling, and (2) the transition, 1Σ+0g↔1Π1g induced by the Coriolis coupling. [ABSTRACT FROM AUTHOR]

Comprehensive analyses of gene expression have been carried out by the development of microarrays and deep sequencers. However, it is difficult to obtain comprehensive information on gene expression from a small amount of ribonucleic acid (RNA). Therefore, we investigated the reproducibility and application of T7 RNA polymerase-mediated transcription, adaptor ligation and polymerase chain reaction (PCR) amplification, followed by T7 transcription (TALPAT), an efficient method for amplifying poly (A)-positive RNA, such as messenger RNA (mRNA). When amplified complementary RNA (cRNA) was electrophoresed, a large number of amplified cRNA was detected in the size of 0.2-0.5 kb. This indicates that the region up to 0.2-0.5 kb from the 3' end of the original mRNA was amplified by the TALPAT method. Seven housekeeping genes, glyceraldehyde-3-phosphate dehydrogenase ( GAPDH ), hydroxymethylbilane synthase ( HMBS ), hypoxanthine phosphoribosyltransferase ( HPRT1 ), ribosomal protein L13a ( RPL13A ), succinate dehydrogenase complex ( SDHA ), TATA box-binding protein ( TBP ) and ubiquitin C ( UBC ), showed high reproducibility (square of the correlation coefficient, R 2 =0.9954), according to scatter plots of Ct values obtained in the real-time PCR analysis of amplified cRNA. In addition, relative expression ratios of amplified cRNA of the seven housekeeping genes were approximately equal to the ratio of the original RNA solution. Furthermore, cRNA was amplified from 20 pg total RNA. In the present study, we confirmed the characteristics of mRNA amplification using the TALPAT method. This method may be applicable to mRNA and poly (A)-positive non-coding RNA amplification, using a small amount of RNA from single, laser-captured and sorted cells, as well as exosomes from serum, urine and body fluids.