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111 results on '"Kimmel, Nils"'

1. Toward atomic resolution diffractive imaging of isolated molecules with x-ray free-electron lasers

Author

Stern, Stephan, Holmegaard, Lotte, Filsinger, Frank, Rouzée, Arnaud, Rudenko, Artem, Johnsson, Per, Martin, Andrew V., Barty, Anton, Bostedt, Christoph, Bozek, John D., Coffee, Ryan N., Epp, Sascha, Erk, Benjamin, Foucar, Lutz, Hartmann, Robert, Kimmel, Nils, Kühnel, Kai-Uwe, Maurer, Jochen, Messerschmidt, Marc, Rudek, Benedikt, Starodub, Dmitri G., Thøgersen, Jan, Weidenspointner, Georg, White, Thomas A., Stapelfeldt, Henrik, Rolles, Daniel, Chapman, Henry N., and Küpper, Jochen

Subjects
Physics - Atomic Physics
Abstract

We give a detailed account of the theoretical analysis and the experimental results of an x-ray-diffraction experiment on quantum-state selected and strongly laser-aligned gas-phase ensembles of the prototypical large asymmetric rotor molecule 2,5-diiodobenzonitrile, performed at the Linac Coherent Light Source [Phys. Rev. Lett. 112, 083002 (2014)]. This experiment is the first step toward coherent diffractive imaging of structures and structural dynamics of isolated molecules at atomic resolution, i. e., picometers and femtoseconds, using x-ray free-electron lasers., Comment: submitted to Faraday Discussions 171 "Emerging Photon Technologies for Chemical Dynamics"

Published
2014
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2. Single-shot diffraction data from the Mimivirus particle using an X-ray free-electron laser

Author

Ekeberg, Tomas, Svenda, Martin, Seibert, M Marvin, Abergel, Chantal, Maia, Filipe RNC, Seltzer, Virginie, DePonte, Daniel P, Aquila, Andrew, Andreasson, Jakob, Iwan, Bianca, Jönsson, Olof, Westphal, Daniel, Odić, Duško, Andersson, Inger, Barty, Anton, Liang, Meng, Martin, Andrew V, Gumprecht, Lars, Fleckenstein, Holger, Bajt, Saša, Barthelmess, Miriam, Coppola, Nicola, Claverie, Jean-Michel, Loh, N Duane, Bostedt, Christoph, Bozek, John D, Krzywinski, Jacek, Messerschmidt, Marc, Bogan, Michael J, Hampton, Christina Y, Sierra, Raymond G, Frank, Matthias, Shoeman, Robert L, Lomb, Lukas, Foucar, Lutz, Epp, Sascha W, Rolles, Daniel, Rudenko, Artem, Hartmann, Robert, Hartmann, Andreas, Kimmel, Nils, Holl, Peter, Weidenspointner, Georg, Rudek, Benedikt, Erk, Benjamin, Kassemeyer, Stephan, Schlichting, Ilme, Strüder, Lothar, Ullrich, Joachim, Schmidt, Carlo, Krasniqi, Faton, Hauser, Günter, Reich, Christian, Soltau, Heike, Schorb, Sebastian, Hirsemann, Helmut, Wunderer, Cornelia, Graafsma, Heinz, Chapman, Henry, and Hajdu, Janos

Subjects
Biochemistry and Cell Biology, Physical Sciences, Biological Sciences, Biomedical Imaging, Algorithms, Computer Simulation, Crystallography, X-Ray, Data Collection, Electrons, Image Processing, Computer-Assisted, Imaging, Three-Dimensional, Lasers, Mimiviridae, Models, Theoretical, Particle Size, Scattering, Radiation, X-Ray Diffraction, X-Rays
Abstract

Free-electron lasers (FEL) hold the potential to revolutionize structural biology by producing X-ray pules short enough to outrun radiation damage, thus allowing imaging of biological samples without the limitation from radiation damage. Thus, a major part of the scientific case for the first FELs was three-dimensional (3D) reconstruction of non-crystalline biological objects. In a recent publication we demonstrated the first 3D reconstruction of a biological object from an X-ray FEL using this technique. The sample was the giant Mimivirus, which is one of the largest known viruses with a diameter of 450 nm. Here we present the dataset used for this successful reconstruction. Data-analysis methods for single-particle imaging at FELs are undergoing heavy development but data collection relies on very limited time available through a highly competitive proposal process. This dataset provides experimental data to the entire community and could boost algorithm development and provide a benchmark dataset for new algorithms.

Published
2016

3. X-ray diffraction from isolated and strongly aligned gas-phase molecules with a free-electron laser

Author

Küpper, Jochen, Stern, Stephan, Holmegaard, Lotte, Filsinger, Frank, Rouzée, Arnaud, Rudenko, Artem, Johnsson, Per, Martin, Andrew V., Adolph, Marcus, Aquila, Andrew, Bajt, Saša, Barty, Anton, Bostedt, Christoph, Bozek, John, Caleman, Carl, Coffee, Ryan, Coppola, Nicola, Delmas, Tjark, Epp, Sascha, Erk, Benjamin, Foucar, Lutz, Gorkhover, Tais, Gumprecht, Lars, Hartmann, Andreas, Hartmann, Robert, Hauser, Günter, Holl, Peter, Hömke, Andre, Kimmel, Nils, Krasniqi, Faton, Kühnel, Kai-Uwe, Maurer, Jochen, Messerschmidt, Marc, Mosshammer, Robert, Reich, Christian, Rudek, Benedikt, Santra, Robin, Schlichting, Ilme, Schmidt, Carlo, Schorb, Sebastian, Schulz, Joachim, Soltau, Heike, Spence, John C. H., Starodub, Dmitri, Strüder, Lothar, Thøgersen, Jan, Vrakking, Marc J. J., Weidenspointner, Georg, White, Thomas A., Wunderer, Cornelia, Meijer, Gerard, Ullrich, Joachim, Stapelfeldt, Henrik, Rolles, Daniel, and Chapman, Henry N.

Subjects
Physics - Atomic Physics
Abstract

We report experimental results on x-ray diffraction of quantum-state-selected and strongly aligned ensembles of the prototypical asymmetric rotor molecule 2,5-diiodobenzonitrile using the Linac Coherent Light Source. The experiments demonstrate first steps toward a new approach to diffractive imaging of distinct structures of individual, isolated gas-phase molecules. We confirm several key ingredients of single molecule diffraction experiments: the abilities to detect and count individual scattered x-ray photons in single shot diffraction data, to deliver state-selected, e. g., structural-isomer-selected, ensembles of molecules to the x-ray interaction volume, and to strongly align the scattering molecules. Our approach, using ultrashort x-ray pulses, is suitable to study ultrafast dynamics of isolated molecules., Comment: submitted to PRL

Published
2013
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4. Time-resolved protein nanocrystallography using an X-ray free-electron laser

Author

Aquila, Andrew, Hunter, Mark S, Doak, R Bruce, Kirian, Richard A, Fromme, Petra, White, Thomas A, Andreasson, Jakob, Arnlund, David, Bajt, Saša, Barends, Thomas RM, Barthelmess, Miriam, Bogan, Michael J, Bostedt, Christoph, Bottin, Hervé, Bozek, John D, Caleman, Carl, Coppola, Nicola, Davidsson, Jan, DePonte, Daniel P, Elser, Veit, Epp, Sascha W, Erk, Benjamin, Fleckenstein, Holger, Foucar, Lutz, Frank, Matthias, Fromme, Raimund, Graafsma, Heinz, Grotjohann, Ingo, Gumprecht, Lars, Hajdu, Janos, Hampton, Christina Y, Hartmann, Andreas, Hartmann, Robert, Hau-Riege, Stefan, Hauser, Günter, Hirsemann, Helmut, Holl, Peter, Holton, James M, Hömke, André, Johansson, Linda, Kimmel, Nils, Kassemeyer, Stephan, Krasniqi, Faton, Kühnel, Kai-Uwe, Liang, Mengning, Lomb, Lukas, Malmerberg, Erik, Marchesini, Stefano, Martin, Andrew V, Maia, Filipe RNC, Messerschmidt, Marc, Nass, Karol, Reich, Christian, Neutze, Richard, Rolles, Daniel, Rudek, Benedikt, Rudenko, Artem, Schlichting, Ilme, Schmidt, Carlo, Schmidt, Kevin E, Schulz, Joachim, Seibert, M Marvin, Shoeman, Robert L, Sierra, Raymond, Soltau, Heike, Starodub, Dmitri, Stellato, Francesco, Stern, Stephan, Strüder, Lothar, Timneanu, Nicusor, Ullrich, Joachim, Wang, Xiaoyu, Williams, Garth J, Weidenspointner, Georg, Weierstall, Uwe, Wunderer, Cornelia, Barty, Anton, Spence, John CH, and Chapman, Henry N

Subjects
Crystallography, X-Ray, Electrons, Ferredoxins, Lasers, Nanostructures, Protein Conformation, X-Ray Diffraction, X-Rays, Optical Physics, Electrical and Electronic Engineering, Communications Technologies, Optics
Abstract

We demonstrate the use of an X-ray free electron laser synchronized with an optical pump laser to obtain X-ray diffraction snapshots from the photoactivated states of large membrane protein complexes in the form of nanocrystals flowing in a liquid jet. Light-induced changes of Photosystem I-Ferredoxin co-crystals were observed at time delays of 5 to 10 µs after excitation. The result correlates with the microsecond kinetics of electron transfer from Photosystem I to ferredoxin. The undocking process that follows the electron transfer leads to large rearrangements in the crystals that will terminally lead to the disintegration of the crystals. We describe the experimental setup and obtain the first time-resolved femtosecond serial X-ray crystallography results from an irreversible photo-chemical reaction at the Linac Coherent Light Source. This technique opens the door to time-resolved structural studies of reaction dynamics in biological systems.

Published
2012

5. CASS—CFEL-ASG software suite

Author

Foucar, Lutz, Barty, Anton, Coppola, Nicola, Hartmann, Robert, Holl, Peter, Hoppe, Uwe, Kassemeyer, Stephan, Kimmel, Nils, Küpper, Jochen, Scholz, Mirko, Techert, Simone, White, Thomas A., Strüder, Lothar, and Ullrich, Joachim

Published
2012
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7. Large-format, high-speed, X-ray pnCCDs combined with electron and ion imaging spectrometers in a multipurpose chamber for experiments at 4th generation light sources

Author

Strüder, Lothar, Epp, Sascha, Rolles, Daniel, Hartmann, Robert, Holl, Peter, Lutz, Gerhard, Soltau, Heike, Eckart, Rouven, Reich, Christian, Heinzinger, Klaus, Thamm, Christian, Rudenko, Artem, Krasniqi, Faton, Kühnel, Kai-Uwe, Bauer, Christian, Schröter, Claus-Dieter, Moshammer, Robert, Techert, Simone, Miessner, Danilo, Porro, Matteo, Hälker, Olaf, Meidinger, Norbert, Kimmel, Nils, Andritschke, Robert, Schopper, Florian, Weidenspointner, Georg, Ziegler, Alexander, Pietschner, Daniel, Herrmann, Sven, Pietsch, Ullrich, Walenta, Albert, Leitenberger, Wolfram, Bostedt, Christoph, Möller, Thomas, Rupp, Daniela, Adolph, Marcus, Graafsma, Heinz, Hirsemann, Helmut, Gärtner, Klaus, Richter, Rainer, Foucar, Lutz, Shoeman, Robert L., Schlichting, Ilme, and Ullrich, Joachim

Published
2010
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10. Femtosecond X-ray protein nanocrystallography

Author

Chapman, Henry N., Fromme, Petra, Barty, Anton, White, Thomas A., Kirian, Richard A., Aquila, Andrew, Hunter, Mark S., Schulz, Joachim, DePonte, Daniel P., Weierstall, Uwe, Doak, Bruce R., Maia, Filipe R. N. C., Martin, Andrew V., Schlichting, Ilme, Lomb, Lukas, Coppola, Nicola, Shoeman, Robert L., Epp, Sascha W., Hartmann, Robert, Rolles, Daniel, Rudenko, Artem, Foucar, Lutz, Kimmel, Nils, Weidenspointner, Georg, Holl, Peter, Liang, Mengning, Barthelmess, Miriam, Caleman, Carl, Boutet, Sébastien, Bogan, Michael J., Krzywinski, Jacek, Bostedt, Christoph, Bajt, Saša, Gumprecht, Lars, Rudek, Benedikt, Erk, Benjamin, Schmidt, Carlo, Hömke, André, Reich, Christian, Pietschner, Daniel, Strüder, Lothar, Hauser, Günter, Gorke, Hubert, Ullrich, Joachim, Herrmann, Sven, Schaller, Gerhard, Schopper, Florian, Soltau, Heike, Kühnel, Kai-Uwe, Messerschmidt, Marc, Bozek, John D., Hau-Riege, Stefan P., Frank, Matthias, Hampton, Christina Y., Sierra, Raymond G., Starodub, Dmitri, Williams, Garth J., Hajdu, Janos, Timneanu, Nicusor, Seibert, Marvin M., Andreasson, Jakob, Rocker, Andrea, Jönsson, Olof, Svenda, Martin, Stern, Stephan, Nass, Karol, Andritschke, Robert, Schröter, Claus-Dieter, Krasniqi, Faton, Bott, Mario, Schmidt, Kevin E., Wang, Xiaoyu, Grotjohann, Ingo, Holton, James M., Barends, Thomas R. M., Neutze, Richard, Marchesini, Stefano, Fromme, Raimund, Schorb, Sebastian, Rupp, Daniela, Adolph, Marcus, Gorkhover, Tais, Andersson, Inger, Hirsemann, Helmut, Potdevin, Guillaume, Graafsma, Heinz, Nilsson, Björn, and Spence, John C. H.

Published
2011
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11. Single mimivirus particles intercepted and imaged with an X-ray laser

Author

Seibert, Marvin M., Ekeberg, Tomas, Maia, Filipe R. N. C., Svenda, Martin, Andreasson, Jakob, Jönsson, Olof, Odić, Duško, Iwan, Bianca, Rocker, Andrea, Westphal, Daniel, Hantke, Max, DePonte, Daniel P., Barty, Anton, Schulz, Joachim, Gumprecht, Lars, Coppola, Nicola, Aquila, Andrew, Liang, Mengning, White, Thomas A., Martin, Andrew, Caleman, Carl, Stern, Stephan, Abergel, Chantal C, Seltzer, Virginie, Claverie, Jean-Michel, Bostedt, Christoph, Bozek, John D., Boutet, Sébastien, Miahnahri, Alan A., Messerschmidt, Marc, Krzywinski, Jacek, Williams, Garth, Hodgson, Keith O., Bogan, Michael J., Hampton, Christina Y., Sierra, Raymond G., Starodub, Dmitri, Andersson, Inger, Bajt, Saša, Barthelmess, Miriam, Spence, John C. H., Fromme, Petra, Weierstall, Uwe, Kirian, Richard, Hunter, Mark, Doak, Bruce R., Marchesini, Stefano, Hau-Riege, Stefan P., Frank, Matthias, Shoeman, Robert L., Lomb, Lukas, Epp, Sascha W., Hartmann, Robert, Rolles, Daniel, Rudenko, Artem, Schmidt, Carlo, Foucar, Lutz, Kimmel, Nils, Holl, Peter, Rudek, Benedikt, Erk, Benjamin, Hömke, André, Reich, Christian, Pietschner, Daniel, Weidenspointner, Georg, Strüder, Lothar, Hauser, Günter, Gorke, Hubert, Ullrich, Joachim, Schlichting, Ilme, Herrmann, Sven, Schaller, Gerhard, Schopper, Florian, Soltau, Heike, Kühnel, Kai-Uwe, Andritschke, Robert, Schröter, Claus-Dieter, Krasniqi, Faton, Bott, Mario, Schorb, Sebastian, Rupp, Daniela, Adolph, Marcus, Gorkhover, Tais, Hirsemann, Helmut, Potdevin, Guillaume, Graafsma, Heinz, Nilsson, Björn, Chapman, Henry N., and Hajdu, Janos J

Published
2011
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12. A data set from flash X-ray imaging of carboxysomes

Author

Hantke, Max F., Hasse, Dirk, Ekeberg, Tomas, John, Katja, Svenda, Martin, Loh, Duane, Martin, Andrew V., Timneanu, Nicusor, Larsson, Daniel S.D., van der Schot, Gijs, Carlsson, Gunilla H., Ingelman, Margareta, Andreasson, Jakob, Westphal, Daniel, Iwan, Bianca, Uetrecht, Charlotte, Bielecki, Johan, Liang, Mengning, Stellato, Francesco, DePonte, Daniel P., Bari, Sadia, Hartmann, Robert, Kimmel, Nils, Kirian, Richard A., Seibert, M. Marvin, Mühlig, Kerstin, Schorb, Sebastian, Ferguson, Ken, Bostedt, Christoph, Carron, Sebastian, Bozek, John D., Rolles, Daniel, Rudenko, Artem, Foucar, Lutz, Epp, Sascha W., Chapman, Henry N., Barty, Anton, Andersson, Inger, Hajdu, Janos, Maia, Filipe R.N.C., Hantke, Max F., Hasse, Dirk, Ekeberg, Tomas, John, Katja, Svenda, Martin, Loh, Duane, Martin, Andrew V., Timneanu, Nicusor, Larsson, Daniel S.D., van der Schot, Gijs, Carlsson, Gunilla H., Ingelman, Margareta, Andreasson, Jakob, Westphal, Daniel, Iwan, Bianca, Uetrecht, Charlotte, Bielecki, Johan, Liang, Mengning, Stellato, Francesco, DePonte, Daniel P., Bari, Sadia, Hartmann, Robert, Kimmel, Nils, Kirian, Richard A., Seibert, M. Marvin, Mühlig, Kerstin, Schorb, Sebastian, Ferguson, Ken, Bostedt, Christoph, Carron, Sebastian, Bozek, John D., Rolles, Daniel, Rudenko, Artem, Foucar, Lutz, Epp, Sascha W., Chapman, Henry N., Barty, Anton, Andersson, Inger, Hajdu, Janos, and Maia, Filipe R.N.C.

Abstract

Ultra-intense femtosecond X-ray pulses from X-ray lasers permit structural studies on single particles and biomolecules without crystals. We present a large data set on inherently heterogeneous, polyhedral carboxysome particles. Carboxysomes are cell organelles that vary in size and facilitate up to 40% of Earth’s carbon fixation by cyanobacteria and certain proteobacteria. Variation in size hinders crystallization. Carboxysomes appear icosahedral in the electron microscope. A protein shell encapsulates a large number of Rubisco molecules in paracrystalline arrays inside the organelle. We used carboxysomes with a mean diameter of 115±26 nm from Halothiobacillus neapolitanus. A new aerosol sample-injector allowed us to record 70,000 low-noise diffraction patterns in 12 min. Every diffraction pattern is a unique structure measurement and high-throughput imaging allows sampling the space of structural variability. The different structures can be separated and phased directly from the diffraction data and open a way for accurate, high-throughput studies on structures and structural heterogeneity in biology and elsewhere., Data Descriptor

Published
2016
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13. Open data set of live cyanobacterial cells imaged using an X-ray laser

Author

van der Schot, Gijs, Svenda, Martin, Maia, Filipe R.N.C., Hantke, Max F., DePonte, Daniel P., Seibert, M. Marvin, Aquila, Andrew, Schulz, Joachim, Kirian, Richard A., Liang, Mengning, Stellato, Francesco, Bari, Sadia, Iwan, Bianca, Andreasson, Jakob, Timneanu, Nicusor, Bielecki, Johan, Westphal, Daniel, Nunes de Almeida, Francisca, Odić, Duško, Hasse, Dirk, Carlsson, Gunilla H., Larsson, Daniel S.D., Barty, Anton, Martin, Andrew V., Schorb, Sebastian, Bostedt, Christoph, Bozek, John D., Carron, Sebastian, Ferguson, Ken, Rolles, Daniel, Rudenko, Artem, Epp, Sascha W., Foucar, Lutz, Rudek, Benedikt, Erk, Benjamin, Hartmann, Robert, Kimmel, Nils, Holl, Peter, Englert, Lars, Loh, N. Duane, Chapman, Henry N., Andersson, Inger, Hajdu, Janos, Ekeberg, Tomas, van der Schot, Gijs, Svenda, Martin, Maia, Filipe R.N.C., Hantke, Max F., DePonte, Daniel P., Seibert, M. Marvin, Aquila, Andrew, Schulz, Joachim, Kirian, Richard A., Liang, Mengning, Stellato, Francesco, Bari, Sadia, Iwan, Bianca, Andreasson, Jakob, Timneanu, Nicusor, Bielecki, Johan, Westphal, Daniel, Nunes de Almeida, Francisca, Odić, Duško, Hasse, Dirk, Carlsson, Gunilla H., Larsson, Daniel S.D., Barty, Anton, Martin, Andrew V., Schorb, Sebastian, Bostedt, Christoph, Bozek, John D., Carron, Sebastian, Ferguson, Ken, Rolles, Daniel, Rudenko, Artem, Epp, Sascha W., Foucar, Lutz, Rudek, Benedikt, Erk, Benjamin, Hartmann, Robert, Kimmel, Nils, Holl, Peter, Englert, Lars, Loh, N. Duane, Chapman, Henry N., Andersson, Inger, Hajdu, Janos, and Ekeberg, Tomas

Abstract

Structural studies on living cells by conventional methods are limited to low resolution because radiation damage kills cells long before the necessary dose for high resolution can be delivered. X-ray free-electron lasers circumvent this problem by outrunning key damage processes with an ultra-short and extremely bright coherent X-ray pulse. Diffraction-before-destruction experiments provide high-resolution data from cells that are alive when the femtosecond X-ray pulse traverses the sample. This paper presents two data sets from micron-sized cyanobacteria obtained at the Linac Coherent Light Source, containing a total of 199,000 diffraction patterns. Utilizing this type of diffraction data will require the development of new analysis methods and algorithms for studying structure and structural variability in large populations of cells and to create abstract models. Such studies will allow us to understand living cells and populations of cells in new ways. New X-ray lasers, like the European XFEL, will produce billions of pulses per day, and could open new areas in structural sciences., Data Descriptor

Published
2016
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14. A data set from flash X-ray imaging of carboxysomes

Author

Hantke, Max F., primary, Hasse, Dirk, additional, Ekeberg, Tomas, additional, John, Katja, additional, Svenda, Martin, additional, Loh, Duane, additional, Martin, Andrew V., additional, Timneanu, Nicusor, additional, Larsson, Daniel S.D., additional, van der Schot, Gijs, additional, Carlsson, Gunilla H., additional, Ingelman, Margareta, additional, Andreasson, Jakob, additional, Westphal, Daniel, additional, Iwan, Bianca, additional, Uetrecht, Charlotte, additional, Bielecki, Johan, additional, Liang, Mengning, additional, Stellato, Francesco, additional, DePonte, Daniel P., additional, Bari, Sadia, additional, Hartmann, Robert, additional, Kimmel, Nils, additional, Kirian, Richard A., additional, Seibert, M. Marvin, additional, Mühlig, Kerstin, additional, Schorb, Sebastian, additional, Ferguson, Ken, additional, Bostedt, Christoph, additional, Carron, Sebastian, additional, Bozek, John D., additional, Rolles, Daniel, additional, Rudenko, Artem, additional, Foucar, Lutz, additional, Epp, Sascha W., additional, Chapman, Henry N., additional, Barty, Anton, additional, Andersson, Inger, additional, Hajdu, Janos, additional, and Maia, Filipe R.N.C., additional

Published
2016
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15. Open data set of live cyanobacterial cells imaged using an X-ray laser

Author

van der Schot, Gijs, primary, Svenda, Martin, additional, Maia, Filipe R.N.C., additional, Hantke, Max F., additional, DePonte, Daniel P., additional, Seibert, M. Marvin, additional, Aquila, Andrew, additional, Schulz, Joachim, additional, Kirian, Richard A., additional, Liang, Mengning, additional, Stellato, Francesco, additional, Bari, Sadia, additional, Iwan, Bianca, additional, Andreasson, Jakob, additional, Timneanu, Nicusor, additional, Bielecki, Johan, additional, Westphal, Daniel, additional, Nunes de Almeida, Francisca, additional, Odić, Duško, additional, Hasse, Dirk, additional, Carlsson, Gunilla H., additional, Larsson, Daniel S.D., additional, Barty, Anton, additional, Martin, Andrew V., additional, Schorb, Sebastian, additional, Bostedt, Christoph, additional, Bozek, John D., additional, Carron, Sebastian, additional, Ferguson, Ken, additional, Rolles, Daniel, additional, Rudenko, Artem, additional, Epp, Sascha W., additional, Foucar, Lutz, additional, Rudek, Benedikt, additional, Erk, Benjamin, additional, Hartmann, Robert, additional, Kimmel, Nils, additional, Holl, Peter, additional, Englert, Lars, additional, Loh, N. Duane, additional, Chapman, Henry N., additional, Andersson, Inger, additional, Hajdu, Janos, additional, and Ekeberg, Tomas, additional

Published
2016
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16. Femtosecond and nanometre visualization of structural dynamics in superheated nanoparticles

Author

Gorkhover, Tais, primary, Schorb, Sebastian, additional, Coffee, Ryan, additional, Adolph, Marcus, additional, Foucar, Lutz, additional, Rupp, Daniela, additional, Aquila, Andrew, additional, Bozek, John D., additional, Epp, Sascha W., additional, Erk, Benjamin, additional, Gumprecht, Lars, additional, Holmegaard, Lotte, additional, Hartmann, Andreas, additional, Hartmann, Robert, additional, Hauser, Günter, additional, Holl, Peter, additional, Hömke, Andre, additional, Johnsson, Per, additional, Kimmel, Nils, additional, Kühnel, Kai-Uwe, additional, Messerschmidt, Marc, additional, Reich, Christian, additional, Rouzée, Arnaud, additional, Rudek, Benedikt, additional, Schmidt, Carlo, additional, Schulz, Joachim, additional, Soltau, Heike, additional, Stern, Stephan, additional, Weidenspointner, Georg, additional, White, Bill, additional, Küpper, Jochen, additional, Strüder, Lothar, additional, Schlichting, Ilme, additional, Ullrich, Joachim, additional, Rolles, Daniel, additional, Rudenko, Artem, additional, Möller, Thomas, additional, and Bostedt, Christoph, additional

Published
2016
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17. Three-dimensional reconstruction of the giant mimivirus particle with an X-ray free-electron laser

Author

Ekeberg, Tomas, Svenda, Martin, Abergel, Chantal, Maia, Filipe R. N. C., Seltzer, Virginie, Claverie, Jean-Michel, Hantke, Max, Jönsson, Olof, Nettelblad, Carl, van der Schot, Gijs, Liang, Mengning, DePonte, Daniel P., Barty, Anton, Seibert, M. Marvin, Iwan, Bianca, Andersson, Inger, Loh, N. Duane, Martin, Andrew V., Chapman, Henry, Bostedt, Christoph, Bozek, John D., Ferguson, Ken R., Krzywinski, Jacek, Epp, Sascha W., Rolles, Daniel, Rudenko, Artem, Hartmann, Robert, Kimmel, Nils, Hajdu, Janos, Ekeberg, Tomas, Svenda, Martin, Abergel, Chantal, Maia, Filipe R. N. C., Seltzer, Virginie, Claverie, Jean-Michel, Hantke, Max, Jönsson, Olof, Nettelblad, Carl, van der Schot, Gijs, Liang, Mengning, DePonte, Daniel P., Barty, Anton, Seibert, M. Marvin, Iwan, Bianca, Andersson, Inger, Loh, N. Duane, Martin, Andrew V., Chapman, Henry, Bostedt, Christoph, Bozek, John D., Ferguson, Ken R., Krzywinski, Jacek, Epp, Sascha W., Rolles, Daniel, Rudenko, Artem, Hartmann, Robert, Kimmel, Nils, and Hajdu, Janos

Published
2015
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18. Imaging single cells in a beam of live cyanobacteria with an X-ray laser

Author

van der Schot, Gijs, Svenda, Martin, Maia, Filipe R. N. C., Hantke, Max, DePonte, Daniel P., Seibert, M. Marvin, Aquila, Andrew, Schulz, Joachim, Kirian, Richard, Liang, Mengning, Stellato, Francesco, Iwan, Bianca, Andreasson, Jakob, Timneanu, Nicusor, Westphal, Daniel, Almeida, F. Nunes, Odic, Dusko, Hasse, Dirk, Carlsson, Gunilla H., Larsson, Daniel S. D., Barty, Anton, Martin, Andrew V., Schorb, Sebastian, Bostedt, Christoph, Bozek, John D., Rolles, Daniel, Rudenko, Artem, Epp, Sascha, Foucar, Lutz, Rudek, Benedikt, Hartmann, Robert, Kimmel, Nils, Holl, Peter, Englert, Lars, Duane Loh, Ne-Te, Chapman, Henry N., Andersson, Inger, Hajdu, Janos, Ekeberg, Tomas, van der Schot, Gijs, Svenda, Martin, Maia, Filipe R. N. C., Hantke, Max, DePonte, Daniel P., Seibert, M. Marvin, Aquila, Andrew, Schulz, Joachim, Kirian, Richard, Liang, Mengning, Stellato, Francesco, Iwan, Bianca, Andreasson, Jakob, Timneanu, Nicusor, Westphal, Daniel, Almeida, F. Nunes, Odic, Dusko, Hasse, Dirk, Carlsson, Gunilla H., Larsson, Daniel S. D., Barty, Anton, Martin, Andrew V., Schorb, Sebastian, Bostedt, Christoph, Bozek, John D., Rolles, Daniel, Rudenko, Artem, Epp, Sascha, Foucar, Lutz, Rudek, Benedikt, Hartmann, Robert, Kimmel, Nils, Holl, Peter, Englert, Lars, Duane Loh, Ne-Te, Chapman, Henry N., Andersson, Inger, Hajdu, Janos, and Ekeberg, Tomas

Abstract

There exists a conspicuous gap of knowledge about the organization of life at mesoscopic levels. Ultra-fast coherent diffractive imaging with X-ray free-electron lasers can probe structures at the relevant length scales and may reach sub-nanometer resolution on micron-sized living cells. Here we show that we can introduce a beam of aerosolised cyanobacteria into the focus of the Linac Coherent Light Source and record diffraction patterns from individual living cells at very low noise levels and at high hit ratios. We obtain two-dimensional projection images directly from the diffraction patterns, and present the results as synthetic X-ray Nomarski images calculated from the complex-valued reconstructions. We further demonstrate that it is possible to record diffraction data to nanometer resolution on live cells with X-ray lasers. Extension to sub-nanometer resolution is within reach, although improvements in pulse parameters and X-ray area detectors will be necessary to unlock this potential.

Published
2015
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19. Three-Dimensional Reconstruction of the Giant Mimivirus Particle with an X-Ray Free-Electron Laser

Author

Ekeberg, Tomas, primary, Svenda, Martin, additional, Abergel, Chantal, additional, Maia, Filipe R. N. C., additional, Seltzer, Virginie, additional, Claverie, Jean-Michel, additional, Hantke, Max, additional, Jönsson, Olof, additional, Nettelblad, Carl, additional, van der Schot, Gijs, additional, Liang, Mengning, additional, DePonte, Daniel P., additional, Barty, Anton, additional, Seibert, M. Marvin, additional, Iwan, Bianca, additional, Andersson, Inger, additional, Loh, N. Duane, additional, Martin, Andrew V., additional, Chapman, Henry, additional, Bostedt, Christoph, additional, Bozek, John D., additional, Ferguson, Ken R., additional, Krzywinski, Jacek, additional, Epp, Sascha W., additional, Rolles, Daniel, additional, Rudenko, Artem, additional, Hartmann, Robert, additional, Kimmel, Nils, additional, and Hajdu, Janos, additional

Published
2015
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20. Analysis of the charge collection process in solid state X-ray detectors

Author

Kimmel, Nils

Subjects
Radiation Detector, X-ray Astronomy, Physics, Röntgenstrahlung, Synchrotronstrahlung, Röntgenastronomie, Synchrotron Radiation, Charge-Coupled-Device, Halbleiter, %22">Beweglichkeit , ddc:530, Ladungsgekoppeltes Bauelement, Electron Mobility, Röntgendetektor, Bauelementephysik
Abstract

Physics with X-rays spans from observing large scales in X-ray astronomy down to small scales in material structure analyses with synchrotron radiation. Both fields of research require imaging detectors featuring spectroscopic resolution for X-rays in an energy range of 0.1keV to 20.0keV. Originally driven by the need for an imaging spectrometer on ESA's X-ray astronomy satellite mission XMM-Newton, X-ray pnCCDs were developed at the semiconductor laboratory of the Max-Planck-Institute. The pnCCD is a pixel array detector made of silicon. It is sensitive over a wide band from near infrared- over optical- and UV-radiation up to X-rays. This thesis describes the dynamics of signal electrons from the moment after their generation until their collection in the potential minima of the pixel structure. Experimentally, a pinhole array was used to scan the pnCCD surface with high spatial resolution. Numerical simulations were used as a tool for the modeling of the electrical conditions inside the pnCCD. The results predicted by the simulations were compared with the measurements. Both, experiment and simulation, helped to establish a model for the signal charge dynamics in the energy range from 0.7keV to 5.5keV. More generally, the presented work has enhanced the understanding of the detector system on the basis of a physical model. The developed experimental and theoretical methods can be applied to any type of array detector which is based on the full depletion of a semiconductor substrate material. Physik mit Röntgenstrahlen befasst sich sowohl mit grosskaligen Strukturen in der Röntgenastronomie, als auch mit kleinskaligen Phänomenen bei Strukturanalysen mit Synchrotronstrahlung. In beiden der genannten Bereiche werden bildgebende Sensoren benötigt, die zusätzlich als Spektrometer im Energiebereich von 0.1keV bis 20.0keV arbeiten. Ursprünglich wurden pnCCDs am Halbleiterlabor der Max-Planck-Institute für Physik und extraterrestrische Physik entwickelt, um ein bildgebendes Spektrometer für die Röntgenastronomiemission XMM-Newton der ESA bereitzustellen. Das pnCCD ist ein Detektor mit Pixelstruktur, der vom Infraroten über optische und UV-Strahlung bis hin zu Röntgenstrahlung empfindlich ist. Diese Arbeit beschreibt die Bewegung von Signalelektronen vom Zeitpunkt ihrer Erzeugung bis zur Sammlung in den Speicherzellen der Pixelstruktur. Zur experimentellen Untersuchung von pnCCDs wurde ein Lochraster eingesetzt, das deren Oberfläche mit hoher räumlicher Auflösung abtastet. Mit Hilfe von numerischen Bauelementesimulationen stand ein Werkzeug zur Modellierung der elektrischen Bedingungen in pnCCDs zur Verfügung. Durch die Kombination der durchgeführten Experimente und Simulationen konnte ein Modell für die Signalladungsdynamik im Energiebereich von 0.7keV bis 5.5keV erstellt werden. Im allgemeineren Sinne hat die vorliegende Arbeit mittels eines physikalisches Modells das Verständnis von pnCCDs erweitert. Die dazu entwickelten experimentellen und theoretischen Methoden können auf jeden Detektor angewendet werden, der auf einem vollständig verarmten Halbleitersubstrat aufbaut.

Published
2009

21. Imaging single cells in a beam of live cyanobacteria with an X-ray laser

Author

van der Schot, Gijs, primary, Svenda, Martin, additional, Maia, Filipe R. N. C., additional, Hantke, Max, additional, DePonte, Daniel P., additional, Seibert, M. Marvin, additional, Aquila, Andrew, additional, Schulz, Joachim, additional, Kirian, Richard, additional, Liang, Mengning, additional, Stellato, Francesco, additional, Iwan, Bianca, additional, Andreasson, Jakob, additional, Timneanu, Nicusor, additional, Westphal, Daniel, additional, Almeida, F. Nunes, additional, Odic, Dusko, additional, Hasse, Dirk, additional, Carlsson, Gunilla H., additional, Larsson, Daniel S. D., additional, Barty, Anton, additional, Martin, Andrew V., additional, Schorb, Sebastian, additional, Bostedt, Christoph, additional, Bozek, John D., additional, Rolles, Daniel, additional, Rudenko, Artem, additional, Epp, Sascha, additional, Foucar, Lutz, additional, Rudek, Benedikt, additional, Hartmann, Robert, additional, Kimmel, Nils, additional, Holl, Peter, additional, Englert, Lars, additional, Duane Loh, Ne-Te, additional, Chapman, Henry N., additional, Andersson, Inger, additional, Hajdu, Janos, additional, and Ekeberg, Tomas, additional

Published
2015
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22. X-Ray Diffraction from Isolated and Strongly Aligned Gas-Phase Molecules with a Free-Electron Laser

Author

Kuepper, Jochen, Stern, Stephan, Holmegaard, Lotte, Filsinger, Frank, Rouzee, Arnaud, Rudenko, Artem, Johnsson, Per, Martin, Andrew V., Adolph, Marcus, Aquila, Andrew, Bajt, Sasa, Barty, Anton, Bostedt, Christoph, Bozek, John, Caleman, Carl, Coffee, Ryan, Coppola, Nicola, Delmas, Tjark, Epp, Sascha, Erk, Benjamin, Foucar, Lutz, Gorkhover, Tais, Gumprecht, Lars, Hartmann, Andreas, Hartmann, Robert, Hauser, Guenter, Holl, Peter, Hoemke, Andre, Kimmel, Nils, Krasniqi, Faton, Kuehnel, Kai-Uwe, Maurer, Jochen, Messerschmidt, Marc, Moshammer, Robert, Reich, Christian, Rudek, Benedikt, Santra, Robin, Schlichting, Ilme, Schmidt, Carlo, Schorb, Sebastian, Schulz, Joachim, Soltau, Heike, Spence, John C. H., Starodub, Dmitri, Strueder, Lothar, Thogersen, Jan, Vrakking, Marc J. J., Weidenspointner, Georg, White, Thomas A., Wunderer, Cornelia, Meijer, Gerard, Ullrich, Joachim, Stapelfeldt, Henrik, Rolles, Daniel, Chapman, Henry N., Kuepper, Jochen, Stern, Stephan, Holmegaard, Lotte, Filsinger, Frank, Rouzee, Arnaud, Rudenko, Artem, Johnsson, Per, Martin, Andrew V., Adolph, Marcus, Aquila, Andrew, Bajt, Sasa, Barty, Anton, Bostedt, Christoph, Bozek, John, Caleman, Carl, Coffee, Ryan, Coppola, Nicola, Delmas, Tjark, Epp, Sascha, Erk, Benjamin, Foucar, Lutz, Gorkhover, Tais, Gumprecht, Lars, Hartmann, Andreas, Hartmann, Robert, Hauser, Guenter, Holl, Peter, Hoemke, Andre, Kimmel, Nils, Krasniqi, Faton, Kuehnel, Kai-Uwe, Maurer, Jochen, Messerschmidt, Marc, Moshammer, Robert, Reich, Christian, Rudek, Benedikt, Santra, Robin, Schlichting, Ilme, Schmidt, Carlo, Schorb, Sebastian, Schulz, Joachim, Soltau, Heike, Spence, John C. H., Starodub, Dmitri, Strueder, Lothar, Thogersen, Jan, Vrakking, Marc J. J., Weidenspointner, Georg, White, Thomas A., Wunderer, Cornelia, Meijer, Gerard, Ullrich, Joachim, Stapelfeldt, Henrik, Rolles, Daniel, and Chapman, Henry N.

Abstract

We report experimental results on x-ray diffraction of quantum-state-selected and strongly aligned ensembles of the prototypical asymmetric rotor molecule 2,5-diiodobenzonitrile using the Linac Coherent Light Source. The experiments demonstrate first steps toward a new approach to diffractive imaging of distinct structures of individual, isolated gas-phase molecules. We confirm several key ingredients of single molecule diffraction experiments: the abilities to detect and count individual scattered x-ray photons in single shot diffraction data, to deliver state-selected, e.g., structural-isomer-selected, ensembles of molecules to the x-ray interaction volume, and to strongly align the scattering molecules. Our approach, using ultrashort x-ray pulses, is suitable to study ultrafast dynamics of isolated molecules.

Published
2014
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23. High-throughput imaging of heterogeneous cell organelles with an X-ray laser

Author

Hantke, Max F., Hasse, Dirk, Maia, Filipe R. N. C., Ekeberg, Tomas, John, Katja, Svenda, Martin, Loh, N. Duane, Martin, Andrew V., Timneanu, Nicusor, Larsson, Daniel S.D., Gijs, van der Schot, Carlsson, Gunilla H., Ingelman, Margareta, Andreasson, Jakob, Westphal, Daniel, Liang, Mengning, Stellato, Francesco, DePonte, Daniel P., Hartmann, Robert, Kimmel, Nils, Kirian, Richard A., Seibert, M. Marvin, Mühlig, Kerstin, Schorb, Sebastian, Ferguson, Ken, Bostedt, Christoph, Carron, Sebastian, Bozek, John D., Rolles, Daniel, Rudenko, Artem, Epp, Sascha, Chapman, Henry N., Barty, Anton, Hajdu, Janos, Andersson, Inger, Hantke, Max F., Hasse, Dirk, Maia, Filipe R. N. C., Ekeberg, Tomas, John, Katja, Svenda, Martin, Loh, N. Duane, Martin, Andrew V., Timneanu, Nicusor, Larsson, Daniel S.D., Gijs, van der Schot, Carlsson, Gunilla H., Ingelman, Margareta, Andreasson, Jakob, Westphal, Daniel, Liang, Mengning, Stellato, Francesco, DePonte, Daniel P., Hartmann, Robert, Kimmel, Nils, Kirian, Richard A., Seibert, M. Marvin, Mühlig, Kerstin, Schorb, Sebastian, Ferguson, Ken, Bostedt, Christoph, Carron, Sebastian, Bozek, John D., Rolles, Daniel, Rudenko, Artem, Epp, Sascha, Chapman, Henry N., Barty, Anton, Hajdu, Janos, and Andersson, Inger

Abstract

We overcome two of the most daunting challenges in single-particle diffractive imaging: collecting many high-quality diffraction patterns on a small amount of sample and separating components from mixed samples. We demonstrate this on carboxysomes, which are polyhedral cell organelles that vary in size and facilitate up to 40% of Earth's carbon fixation. A new aerosol sample-injector allowed us to record 70,000 low-noise diffraction patterns in 12 min with the Linac Coherent Light Source running at 120 Hz. We separate different structures directly from the diffraction data and show that the size distribution is preserved during sample delivery. We automate phase retrieval and avoid reconstruction artefacts caused by missing modes. We attain the highest-resolution reconstructions on the smallest single biological objects imaged with an X-ray laser to date. These advances lay the foundations for accurate, high-throughput structure determination by flash-diffractive imaging and offer a means to study structure and structural heterogeneity in biology and elsewhere.

Published
2014
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24. High-throughput imaging of heterogeneous cell organelles with an X-ray laser

Author

Hantke, Max F., primary, Hasse, Dirk, additional, Maia, Filipe R. N. C., additional, Ekeberg, Tomas, additional, John, Katja, additional, Svenda, Martin, additional, Loh, N. Duane, additional, Martin, Andrew V., additional, Timneanu, Nicusor, additional, Larsson, Daniel S. D., additional, van der Schot, Gijs, additional, Carlsson, Gunilla H., additional, Ingelman, Margareta, additional, Andreasson, Jakob, additional, Westphal, Daniel, additional, Liang, Mengning, additional, Stellato, Francesco, additional, DePonte, Daniel P., additional, Hartmann, Robert, additional, Kimmel, Nils, additional, Kirian, Richard A., additional, Seibert, M. Marvin, additional, Mühlig, Kerstin, additional, Schorb, Sebastian, additional, Ferguson, Ken, additional, Bostedt, Christoph, additional, Carron, Sebastian, additional, Bozek, John D., additional, Rolles, Daniel, additional, Rudenko, Artem, additional, Epp, Sascha, additional, Chapman, Henry N., additional, Barty, Anton, additional, Hajdu, Janos, additional, and Andersson, Inger, additional

Published
2014
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25. Sensing the wavefront of x-ray free-electron lasers using aerosol spheres

Author

Loh, N. Duane, Starodub, Dmitri, Lomb, Lukas, Hampton, Christina Y., Martin, Andrew V., Sierra, Raymond G., Barty, Anton, Aquila, Andrew, Schulz, Joachim, Steinbrener, Jan, Shoeman, Robert L., Kassemeyer, Stephan, Bostedt, Christoph, Bozek, John, Epp, Sascha W., Erk, Benjamin, Hartmann, Robert, Rolles, Daniel, Rudenko, Artem, Rudek, Benedikt, Foucar, Lutz, Kimmel, Nils, Weidenspointner, Georg, Hauser, Guenter, Holl, Peter, Pedersoli, Emanuele, Liang, Mengning, Hunter, Mark S., Gumprecht, Lars, Coppola, Nicola, Wunderer, Cornelia, Graafsma, Heinz, Maia, Filipe R. N. C., Ekeberg, Tomas, Hantke, Max Felix, Fleckenstein, Holger, Hirsemann, Helmut, Nass, Karol, White, Thomas A., Tobias, Herbert J., Farquar, George R., Benner, W. Henry, Hau-Riege, Stefan, Reich, Christian, Hartmann, Andreas, Soltau, Heike, Marchesini, Stefano, Bajt, Sasa, Barthelmess, Miriam, Strueder, Lothar, Ullrich, Joachim, Bucksbaum, Philip, Frank, Matthias, Schlichting, Ilme, Chapman, Henry N., Bogan, Michael J., Loh, N. Duane, Starodub, Dmitri, Lomb, Lukas, Hampton, Christina Y., Martin, Andrew V., Sierra, Raymond G., Barty, Anton, Aquila, Andrew, Schulz, Joachim, Steinbrener, Jan, Shoeman, Robert L., Kassemeyer, Stephan, Bostedt, Christoph, Bozek, John, Epp, Sascha W., Erk, Benjamin, Hartmann, Robert, Rolles, Daniel, Rudenko, Artem, Rudek, Benedikt, Foucar, Lutz, Kimmel, Nils, Weidenspointner, Georg, Hauser, Guenter, Holl, Peter, Pedersoli, Emanuele, Liang, Mengning, Hunter, Mark S., Gumprecht, Lars, Coppola, Nicola, Wunderer, Cornelia, Graafsma, Heinz, Maia, Filipe R. N. C., Ekeberg, Tomas, Hantke, Max Felix, Fleckenstein, Holger, Hirsemann, Helmut, Nass, Karol, White, Thomas A., Tobias, Herbert J., Farquar, George R., Benner, W. Henry, Hau-Riege, Stefan, Reich, Christian, Hartmann, Andreas, Soltau, Heike, Marchesini, Stefano, Bajt, Sasa, Barthelmess, Miriam, Strueder, Lothar, Ullrich, Joachim, Bucksbaum, Philip, Frank, Matthias, Schlichting, Ilme, Chapman, Henry N., and Bogan, Michael J.

Abstract

Characterizing intense, focused x-ray free electron laser (FEL) pulses is crucial for their use in diffractive imaging. We describe how the distribution of average phase tilts and intensities on hard x-ray pulses with peak intensities of 1021 W/m(2) can be retrieved from an ensemble of diffraction patterns produced by 70 nm-radius polystyrene spheres, in a manner that mimics wavefront sensors. Besides showing that an adaptive geometric correction may be necessary for diffraction data from randomly injected sample sources, our paper demonstrates the possibility of collecting statistics on structured pulses using only the diffraction patterns they generate and highlights the imperative to study its impact on single-particle diffractive imaging.

Published
2013
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26. Toward unsupervised single-shot diffractive imaging of heterogeneous particles using X-ray free-electron lasers

Author

Park, Hyung Joo, Loh, N. Duane, Sierra, Raymond G., Hampton, Christina Y., Starodub, Dmitri, Martin, Andrew V., Barty, Anton, Aquila, Andrew, Schulz, Joachim, Steinbrener, Jan, Shoeman, Robert L., Lomb, Lukas, Kassemeyer, Stephan, Bostedt, Christoph, Bozek, John, Epp, Sascha W., Erk, Benjamin, Hartmann, Robert, Rolles, Daniel, Rudenko, Artem, Rudek, Benedikt, Foucar, Lutz, Kimmel, Nils, Weidenspointner, Georg, Hauser, Guenter, Holl, Peter, Pedersoli, Emanuele, Liang, Mengning, Hunter, Mark S., Gumprecht, Lars, Coppola, Nicola, Wunderer, Cornelia, Graafsma, Heinz, Maia, Filipe R. N. C., Ekeberg, Tomas, Hantke, Max Felix, Fleckenstein, Holger, Hirsemann, Helmut, Nass, Karol, Tobias, Herbert J., Farquar, George R., Benner, W. Henry, Hau-Riege, Stefan, Reich, Christian, Hartmann, Andreas, Soltau, Heike, Marchesini, Stefano, Bajt, Sasa, Barthelmess, Miriam, Strueder, Lothar, Ullrich, Joachim, Bucksbaum, Philip, Frank, Matthias, Schlichting, Ilme, Chapman, Henry N., Bogan, Michael J., Elser, Veit, Park, Hyung Joo, Loh, N. Duane, Sierra, Raymond G., Hampton, Christina Y., Starodub, Dmitri, Martin, Andrew V., Barty, Anton, Aquila, Andrew, Schulz, Joachim, Steinbrener, Jan, Shoeman, Robert L., Lomb, Lukas, Kassemeyer, Stephan, Bostedt, Christoph, Bozek, John, Epp, Sascha W., Erk, Benjamin, Hartmann, Robert, Rolles, Daniel, Rudenko, Artem, Rudek, Benedikt, Foucar, Lutz, Kimmel, Nils, Weidenspointner, Georg, Hauser, Guenter, Holl, Peter, Pedersoli, Emanuele, Liang, Mengning, Hunter, Mark S., Gumprecht, Lars, Coppola, Nicola, Wunderer, Cornelia, Graafsma, Heinz, Maia, Filipe R. N. C., Ekeberg, Tomas, Hantke, Max Felix, Fleckenstein, Holger, Hirsemann, Helmut, Nass, Karol, Tobias, Herbert J., Farquar, George R., Benner, W. Henry, Hau-Riege, Stefan, Reich, Christian, Hartmann, Andreas, Soltau, Heike, Marchesini, Stefano, Bajt, Sasa, Barthelmess, Miriam, Strueder, Lothar, Ullrich, Joachim, Bucksbaum, Philip, Frank, Matthias, Schlichting, Ilme, Chapman, Henry N., Bogan, Michael J., and Elser, Veit

Abstract

Single shot diffraction imaging experiments via X-ray free-electron lasers can generate as many as hundreds of thousands of diffraction patterns of scattering objects. Recovering the real space contrast of a scattering object from these patterns currently requires a reconstruction process with user guidance in a number of steps, introducing severe bottlenecks in data processing. We present a series of measures that replace user guidance with algorithms that reconstruct contrasts in an unsupervised fashion. We demonstrate the feasibility of automating the reconstruction process by generating hundreds of contrasts obtained from soot particle diffraction experiments.

Published
2013
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27. Self-terminating diffraction gates femtosecond X-ray nanocrystallography measurements

Author

Barty, Anton, Caleman, Carl, Aquila, Andrew, Timneanu, Nicusor, Lomb, Lukas, White, Thomas A., Andreasson, Jakob, Arnlund, David, Bajt, Sasa, Barends, Thomas R. M., Barthelmess, Miriam, Bogan, Michael J., Bostedt, Christoph, Bozek, John D., Coffee, Ryan, Coppola, Nicola, Davidsson, Jan, DePonte, Daniel P., Doak, R. Bruce, Ekeberg, Tomas, Elser, Veit, Epp, Sascha W., Erk, Benjamin, Fleckenstein, Holger, Foucar, Lutz, Fromme, Petra, Graafsma, Heinz, Gumprecht, Lars, Hajdu, Janos, Hampton, Christina Y., Hartmann, Robert, Hartmann, Andreas, Hauser, Guenter, Hirsemann, Helmut, Holl, Peter, Hunter, Mark S., Johansson, Linda, Kassemeyer, Stephan, Kimmel, Nils, Kirian, Richard A., Liang, Mengning, Maia, Filipe R. N. C., Malmerberg, Erik, Marchesini, Stefano, Martin, Andrew V., Nass, Karol, Neutze, Richard, Reich, Christian, Rolles, Daniel, Rudek, Benedikt, Rudenko, Artem, Scott, Howard, Schlichting, Ilme, Schulz, Joachim, Seibert, M. Marvin, Shoeman, Robert L., Sierra, Raymond G., Soltau, Heike, Spence, John C. H., Stellato, Francesco, Stern, Stephan, Strueder, Lothar, Ullrich, Joachim, Wang, X., Weidenspointner, Georg, Weierstall, Uwe, Wunderer, Cornelia B., Chapman, Henry N., Barty, Anton, Caleman, Carl, Aquila, Andrew, Timneanu, Nicusor, Lomb, Lukas, White, Thomas A., Andreasson, Jakob, Arnlund, David, Bajt, Sasa, Barends, Thomas R. M., Barthelmess, Miriam, Bogan, Michael J., Bostedt, Christoph, Bozek, John D., Coffee, Ryan, Coppola, Nicola, Davidsson, Jan, DePonte, Daniel P., Doak, R. Bruce, Ekeberg, Tomas, Elser, Veit, Epp, Sascha W., Erk, Benjamin, Fleckenstein, Holger, Foucar, Lutz, Fromme, Petra, Graafsma, Heinz, Gumprecht, Lars, Hajdu, Janos, Hampton, Christina Y., Hartmann, Robert, Hartmann, Andreas, Hauser, Guenter, Hirsemann, Helmut, Holl, Peter, Hunter, Mark S., Johansson, Linda, Kassemeyer, Stephan, Kimmel, Nils, Kirian, Richard A., Liang, Mengning, Maia, Filipe R. N. C., Malmerberg, Erik, Marchesini, Stefano, Martin, Andrew V., Nass, Karol, Neutze, Richard, Reich, Christian, Rolles, Daniel, Rudek, Benedikt, Rudenko, Artem, Scott, Howard, Schlichting, Ilme, Schulz, Joachim, Seibert, M. Marvin, Shoeman, Robert L., Sierra, Raymond G., Soltau, Heike, Spence, John C. H., Stellato, Francesco, Stern, Stephan, Strueder, Lothar, Ullrich, Joachim, Wang, X., Weidenspointner, Georg, Weierstall, Uwe, Wunderer, Cornelia B., and Chapman, Henry N.

Abstract

X-ray free-electron lasers have enabled new approaches to the structural determination of protein crystals that are too small or radiation-sensitive for conventional analysis(1). For sufficiently short pulses, diffraction is collected before significant changes occur to the sample, and it has been predicted that pulses as short as 10 fs may be required to acquire atomic-resolution structural information(1-4). Here, we describe a mechanism unique to ultrafast, ultra-intense X-ray experiments that allows structural information to be collected from crystalline samples using high radiation doses without the requirement for the pulse to terminate before the onset of sample damage. Instead, the diffracted X-rays are gated by a rapid loss of crystalline periodicity, producing apparent pulse lengths significantly shorter than the duration of the incident pulse. The shortest apparent pulse lengths occur at the highest resolution, and our measurements indicate that current X-ray free-electron laser technology(5) should enable structural determination from submicrometre protein crystals with atomic resolution.

Published
2012
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28. Lipidic phase membrane protein serial femtosecond crystallography

Author

Johansson, Linda C, Arnlund, David, White, Thomas A, Katona, Gergely, DePonte, Daniel P, Weierstall, Uwe, Doak, R Bruce, Shoeman, Robert L, Lomb, Lukas, Malmerberg, Erik, Davidsson, Jan, Nass, Karol, Liang, Mengning, Andreasson, Jakob, Aquila, Andrew, Bajt, Sasa, Barthelmess, Miriam, Barty, Anton, Bogan, Michael J, Bostedt, Christoph, Bozek, John D, Caleman, Carl, Coffee, Ryan, Coppola, Nicola, Ekeberg, Tomas, Epp, Sascha W, Erk, Benjamin, Fleckenstein, Holger, Foucar, Lutz, Graafsma, Heinz, Gumprecht, Lars, Hajdu, Janos, Hampton, Christina Y, Hartmann, Robert, Hartmann, Andreas, Hauser, Gunter, Hirsemann, Helmut, Holl, Peter, Hunter, Mark S, Kassemeyer, Stephan, Kimmel, Nils, Kirian, Richard A, Maia, Filipe R N C, Marchesini, Stefano, Martin, Andrew V, Reich, Christian, Rolles, Daniel, Rudek, Benedikt, Rudenko, Artem, Schlichting, Ilme, Schulz, Joachim, Seibert, M Marvin, Sierra, Raymond G, Soltau, Heike, Starodub, Dmitri, Stellato, Francesco, Stern, Stephan, Struder, Lothar, Timneanu, Nicusor, Ullrich, Joachim, Wahlgren, Weixiao Y, Wang, Xiaoyu, Weidenspointner, Georg, Wunderer, Cornelia, Fromme, Petra, Chapman, Henry N, Spence, John C H, Neutze, Richard, Johansson, Linda C, Arnlund, David, White, Thomas A, Katona, Gergely, DePonte, Daniel P, Weierstall, Uwe, Doak, R Bruce, Shoeman, Robert L, Lomb, Lukas, Malmerberg, Erik, Davidsson, Jan, Nass, Karol, Liang, Mengning, Andreasson, Jakob, Aquila, Andrew, Bajt, Sasa, Barthelmess, Miriam, Barty, Anton, Bogan, Michael J, Bostedt, Christoph, Bozek, John D, Caleman, Carl, Coffee, Ryan, Coppola, Nicola, Ekeberg, Tomas, Epp, Sascha W, Erk, Benjamin, Fleckenstein, Holger, Foucar, Lutz, Graafsma, Heinz, Gumprecht, Lars, Hajdu, Janos, Hampton, Christina Y, Hartmann, Robert, Hartmann, Andreas, Hauser, Gunter, Hirsemann, Helmut, Holl, Peter, Hunter, Mark S, Kassemeyer, Stephan, Kimmel, Nils, Kirian, Richard A, Maia, Filipe R N C, Marchesini, Stefano, Martin, Andrew V, Reich, Christian, Rolles, Daniel, Rudek, Benedikt, Rudenko, Artem, Schlichting, Ilme, Schulz, Joachim, Seibert, M Marvin, Sierra, Raymond G, Soltau, Heike, Starodub, Dmitri, Stellato, Francesco, Stern, Stephan, Struder, Lothar, Timneanu, Nicusor, Ullrich, Joachim, Wahlgren, Weixiao Y, Wang, Xiaoyu, Weidenspointner, Georg, Wunderer, Cornelia, Fromme, Petra, Chapman, Henry N, Spence, John C H, and Neutze, Richard

Abstract

X-ray free electron laser (X-FEL)-based serial femtosecond crystallography is an emerging method with potential to rapidly advance the challenging field of membrane protein structural biology. Here we recorded interpretable diffraction data from micrometer-sized lipidic sponge phase crystals of the Blastochloris viridis photosynthetic reaction center delivered into an X-FEL beam using a sponge phase micro-jet.

Published
2012
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29. In vivo protein crystallization opens new routes in structural biology

Author

Koopmann, Rudolf, Cupelli, Karolina, Redecke, Lars, Nass, Karol, DePonte, Daniel P, White, Thomas A, Stellato, Francesco, Rehders, Dirk, Liang, Mengning, Andreasson, Jakob, Aquila, Andrew, Bajt, Sasa, Barthelmess, Miriam, Barty, Anton, Bogan, Michael J, Bostedt, Christoph, Boutet, Sebastien, Bozek, John D, Caleman, Carl, Coppola, Nicola, Davidsson, Jan, Doak, R Bruce, Ekeberg, Tomas, Epp, Sascha W, Erk, Benjamin, Fleckenstein, Holger, Foucar, Lutz, Graafsma, Heinz, Gumprecht, Lars, Hajdu, Janos, Hampton, Christina Y, Hartmann, Andreas, Hartmann, Robert, Hauser, Gunter, Hirsemann, Helmut, Holl, Peter, Hunter, Mark S, Kassemeyer, Stephan, Kirian, Richard A, Lomb, Lukas, Maia, Filipe R N C, Kimmel, Nils, Martin, Andrew V, Messerschmidt, Marc, Reich, Christian, Rolles, Daniel, Rudek, Benedikt, Rudenko, Artem, Schlichting, Ilme, Schulz, Joachim, Seibert, M Marvin, Shoeman, Robert L, Sierra, Raymond G, Soltau, Heike, Stern, Stephan, Struder, Lothar, Timneanu, Nicusor, Ullrich, Joachim, Wang, Xiaoyu, Weidenspointner, Georg, Weierstall, Uwe, Williams, Garth J, Wunderer, Cornelia B, Fromme, Petra, Spence, John C H, Stehle, Thilo, Chapman, Henry N, Betzel, Christian, Duszenko, Michael, Koopmann, Rudolf, Cupelli, Karolina, Redecke, Lars, Nass, Karol, DePonte, Daniel P, White, Thomas A, Stellato, Francesco, Rehders, Dirk, Liang, Mengning, Andreasson, Jakob, Aquila, Andrew, Bajt, Sasa, Barthelmess, Miriam, Barty, Anton, Bogan, Michael J, Bostedt, Christoph, Boutet, Sebastien, Bozek, John D, Caleman, Carl, Coppola, Nicola, Davidsson, Jan, Doak, R Bruce, Ekeberg, Tomas, Epp, Sascha W, Erk, Benjamin, Fleckenstein, Holger, Foucar, Lutz, Graafsma, Heinz, Gumprecht, Lars, Hajdu, Janos, Hampton, Christina Y, Hartmann, Andreas, Hartmann, Robert, Hauser, Gunter, Hirsemann, Helmut, Holl, Peter, Hunter, Mark S, Kassemeyer, Stephan, Kirian, Richard A, Lomb, Lukas, Maia, Filipe R N C, Kimmel, Nils, Martin, Andrew V, Messerschmidt, Marc, Reich, Christian, Rolles, Daniel, Rudek, Benedikt, Rudenko, Artem, Schlichting, Ilme, Schulz, Joachim, Seibert, M Marvin, Shoeman, Robert L, Sierra, Raymond G, Soltau, Heike, Stern, Stephan, Struder, Lothar, Timneanu, Nicusor, Ullrich, Joachim, Wang, Xiaoyu, Weidenspointner, Georg, Weierstall, Uwe, Williams, Garth J, Wunderer, Cornelia B, Fromme, Petra, Spence, John C H, Stehle, Thilo, Chapman, Henry N, Betzel, Christian, and Duszenko, Michael

Abstract

Protein crystallization in cells has been observed several times in nature. However, owing to their small size these crystals have not yet been used for X-ray crystallographic analysis. We prepared nano-sized in vivo–grown crystals of Trypanosoma brucei enzymes and applied the emerging method of free-electron laser-based serial femtosecond crystallography to record interpretable diffraction data. This combined approach will open new opportunities in structural systems biology.

Published
2012
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30. Femtosecond free-electron laser x-ray diffraction data sets for algorithm development

Author

Kassemeyer, Stephan, Steinbrener, Jan, Lomb, Lukas, Hartmann, Elisabeth, Aquila, Andrew, Barty, Anton, Martin, Andrew V, Hampton, Christina Y, Bajt, Saša, Barthelmess, Miriam, Barends, Thomas R M, Bostedt, Christoph, Bott, Mario, Bozek, John D, Coppola, Nicola, Cryle, Max, Deponte, Daniel P, Doak, R Bruce, Epp, Sascha W, Erk, Benjamin, Fleckenstein, Holger, Foucar, Lutz, Graafsma, Heinz, Gumprecht, Lars, Hartmann, Andreas, Hartmann, Robert, Hauser, Günter, Hirsemann, Helmut, Hömke, André, Holl, Peter, Jönsson, Olof, Kimmel, Nils, Krasniqi, Faton, Liang, Mengning, Maia, Filipe R N C, Marchesini, Stefano, Nass, Karol, Reich, Christian, Rolles, Daniel, Rudek, Benedikt, Rudenko, Artem, Schmidt, Carlo, Schulz, Joachim, Shoeman, Robert L, Sierra, Raymond G, Soltau, Heike, Spence, John C H, Starodub, Dmitri, Stellato, Francesco, Stern, Stephan, Stier, Gunter, Svenda, Martin, Weidenspointner, Georg, Weierstall, Uwe, White, Thomas A, Wunderer, Cornelia, Frank, Matthias, Chapman, Henry N, Ullrich, Joachim, Strüder, Lothar, Bogan, Michael J, Schlichting, Ilme, Kassemeyer, Stephan, Steinbrener, Jan, Lomb, Lukas, Hartmann, Elisabeth, Aquila, Andrew, Barty, Anton, Martin, Andrew V, Hampton, Christina Y, Bajt, Saša, Barthelmess, Miriam, Barends, Thomas R M, Bostedt, Christoph, Bott, Mario, Bozek, John D, Coppola, Nicola, Cryle, Max, Deponte, Daniel P, Doak, R Bruce, Epp, Sascha W, Erk, Benjamin, Fleckenstein, Holger, Foucar, Lutz, Graafsma, Heinz, Gumprecht, Lars, Hartmann, Andreas, Hartmann, Robert, Hauser, Günter, Hirsemann, Helmut, Hömke, André, Holl, Peter, Jönsson, Olof, Kimmel, Nils, Krasniqi, Faton, Liang, Mengning, Maia, Filipe R N C, Marchesini, Stefano, Nass, Karol, Reich, Christian, Rolles, Daniel, Rudek, Benedikt, Rudenko, Artem, Schmidt, Carlo, Schulz, Joachim, Shoeman, Robert L, Sierra, Raymond G, Soltau, Heike, Spence, John C H, Starodub, Dmitri, Stellato, Francesco, Stern, Stephan, Stier, Gunter, Svenda, Martin, Weidenspointner, Georg, Weierstall, Uwe, White, Thomas A, Wunderer, Cornelia, Frank, Matthias, Chapman, Henry N, Ullrich, Joachim, Strüder, Lothar, Bogan, Michael J, and Schlichting, Ilme

Abstract

We describe femtosecond X-ray diffraction data sets of viruses and nanoparticles collected at the Linac Coherent Light Source. The data establish the first large benchmark data sets for coherent diffraction methods freely available to the public, to bolster the development of algorithms that are essential for developing this novel approach as a useful imaging technique. Applications are 2D reconstructions, orientation classification and finally 3D imaging by assembling 2D patterns into a 3D diffraction volume.

Published
2012
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31. Single mimivirus particles intercepted and imaged with an X-ray laser

Author

Seibert, M. Marvin, Ekeberg, Tomas, Maia, Filipe R. N. C., Svenda, Martin, Andreasson, Jakob, Joensson, Olof, Odic, Dusko, Iwan, Bianca, Rocker, Andrea, Westphal, Daniel, Hantke, Max, DePonte, Daniel P., Barty, Anton, Schulz, Joachim, Gumprecht, Lars, Coppola, Nicola, Aquila, Andrew, Liang, Mengning, White, Thomas A., Martin, Andrew, Caleman, Carl, Stern, Stephan, Abergel, Chantal, Seltzer, Virginie, Claverie, Jean-Michel, Bostedt, Christoph, Bozek, John D., Boutet, Sebastien, Miahnahri, A. Alan, Messerschmidt, Marc, Krzywinski, Jacek, Williams, Garth, Hodgson, Keith O., Bogan, Michael J., Hampton, Christina Y., Sierra, Raymond G., Starodub, Dmitri, Andersson, Inger, Bajt, Sasa, Barthelmess, Miriam, Spence, John C. H., Fromme, Petra, Weierstall, Uwe, Kirian, Richard, Hunter, Mark, Doak, R. Bruce, Marchesini, Stefano, Hau-Riege, Stefan P., Frank, Matthias, Shoeman, Robert L., Lomb, Lukas, Epp, Sascha W., Hartmann, Robert, Rolles, Daniel, Rudenko, Artem, Schmidt, Carlo, Foucar, Lutz, Kimmel, Nils, Holl, Peter, Rudek, Benedikt, Erk, Benjamin, Hoemke, Andre, Reich, Christian, Pietschner, Daniel, Weidenspointner, Georg, Strueder, Lothar, Hauser, Guenter, Gorke, Hubert, Ullrich, Joachim, Schlichting, Ilme, Herrmann, Sven, Schaller, Gerhard, Schopper, Florian, Soltau, Heike, Kuehnel, Kai-Uwe, Andritschke, Robert, Schroeter, Claus-Dieter, Krasniqi, Faton, Bott, Mario, Schorb, Sebastian, Rupp, Daniela, Adolph, Marcus, Gorkhover, Tais, Hirsemann, Helmut, Potdevin, Guillaume, Graafsma, Heinz, Nilsson, Björn, Chapman, Henry N., Hajdu, Janos, Seibert, M. Marvin, Ekeberg, Tomas, Maia, Filipe R. N. C., Svenda, Martin, Andreasson, Jakob, Joensson, Olof, Odic, Dusko, Iwan, Bianca, Rocker, Andrea, Westphal, Daniel, Hantke, Max, DePonte, Daniel P., Barty, Anton, Schulz, Joachim, Gumprecht, Lars, Coppola, Nicola, Aquila, Andrew, Liang, Mengning, White, Thomas A., Martin, Andrew, Caleman, Carl, Stern, Stephan, Abergel, Chantal, Seltzer, Virginie, Claverie, Jean-Michel, Bostedt, Christoph, Bozek, John D., Boutet, Sebastien, Miahnahri, A. Alan, Messerschmidt, Marc, Krzywinski, Jacek, Williams, Garth, Hodgson, Keith O., Bogan, Michael J., Hampton, Christina Y., Sierra, Raymond G., Starodub, Dmitri, Andersson, Inger, Bajt, Sasa, Barthelmess, Miriam, Spence, John C. H., Fromme, Petra, Weierstall, Uwe, Kirian, Richard, Hunter, Mark, Doak, R. Bruce, Marchesini, Stefano, Hau-Riege, Stefan P., Frank, Matthias, Shoeman, Robert L., Lomb, Lukas, Epp, Sascha W., Hartmann, Robert, Rolles, Daniel, Rudenko, Artem, Schmidt, Carlo, Foucar, Lutz, Kimmel, Nils, Holl, Peter, Rudek, Benedikt, Erk, Benjamin, Hoemke, Andre, Reich, Christian, Pietschner, Daniel, Weidenspointner, Georg, Strueder, Lothar, Hauser, Guenter, Gorke, Hubert, Ullrich, Joachim, Schlichting, Ilme, Herrmann, Sven, Schaller, Gerhard, Schopper, Florian, Soltau, Heike, Kuehnel, Kai-Uwe, Andritschke, Robert, Schroeter, Claus-Dieter, Krasniqi, Faton, Bott, Mario, Schorb, Sebastian, Rupp, Daniela, Adolph, Marcus, Gorkhover, Tais, Hirsemann, Helmut, Potdevin, Guillaume, Graafsma, Heinz, Nilsson, Björn, Chapman, Henry N., and Hajdu, Janos

Abstract

X-ray lasers offer new capabilities in understanding the structure of biological systems, complex materials and matter under extreme conditions(1-4). Very short and extremely bright, coherent X-ray pulses can be used to outrun key damage processes and obtain a single diffraction pattern from a large macromolecule, a virus or a cell before the sample explodes and turns into plasma(1). The continuous diffraction pattern of non-crystalline objects permits oversampling and direct phase retrieval(2). Here we show that high-quality diffraction data can be obtained with a single X-ray pulse from a noncrystalline biological sample, a single mimivirus particle, which was injected into the pulsed beam of a hard-X-ray free-electron laser, the Linac Coherent Light Source(5). Calculations indicate that the energy deposited into the virus by the pulse heated the particle to over 100,000 K after the pulse had left the sample. The reconstructed exit wavefront (image) yielded 32-nm full-period resolution in a single exposure and showed no measurable damage. The reconstruction indicates inhomogeneous arrangement of dense material inside the virion. We expect that significantly higher resolutions will be achieved in such experiments with shorter and brighter photon pulses focused to a smaller area. The resolution in such experiments can be further extended for samples available in multiple identical copies.

Published
2011
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32. Unsupervised classification of single-particle X-ray diffraction snapshots by spectral clustering

Author

Yoon, Chun Hong, Schwander, Peter, Abergel, Chantal, Andersson, Inger, Andreasson, Jakob, Aquila, Andrew, Bajt, Sasa, Barthelmess, Miriam, Barty, Anton, Bogan, Michael J., Bostedt, Christoph, Bozek, John, Chapman, Henry N., Claverie, Jean-Michel, Coppola, Nicola, DePonte, Daniel P., Ekeberg, Tomas, Epp, Sascha W., Erk, Benjamin, Fleckenstein, Holger, Foucar, Lutz, Graafsma, Heinz, Gumprecht, Lars, Hajdu, Janos, Hampton, Christina Y., Hartmann, Andreas, Hartmann, Elisabeth, Hartmann, Robert, Hauser, Gunter, Hirsemann, Helmut, Holl, Peter, Kassemeyer, Stephan, Kimmel, Nils, Kiskinova, Maya, Liang, Mengning, Loh, Ne-Te Duane, Lomb, Lukas, Maia, Filipe R. N. C., Martin, Andrew V., Nass, Karol, Pedersoli, Emanuele, Reich, Christian, Rolles, Daniel, Rudek, Benedikt, Rudenko, Artem, Schlichting, Ilme, Schulz, Joachim, Seibert, Marvin, Seltzer, Virginie, Shoeman, Robert L., Sierra, Raymond G., Soltau, Heike, Starodub, Dmitri, Steinbrener, Jan, Stier, Gunter, Strueder, Lothar, Svenda, Martin, Ullrich, Joachim, Weidenspointner, Georg, White, Thomas A., Wunderer, Cornelia, Ourmazd, Abbas, Yoon, Chun Hong, Schwander, Peter, Abergel, Chantal, Andersson, Inger, Andreasson, Jakob, Aquila, Andrew, Bajt, Sasa, Barthelmess, Miriam, Barty, Anton, Bogan, Michael J., Bostedt, Christoph, Bozek, John, Chapman, Henry N., Claverie, Jean-Michel, Coppola, Nicola, DePonte, Daniel P., Ekeberg, Tomas, Epp, Sascha W., Erk, Benjamin, Fleckenstein, Holger, Foucar, Lutz, Graafsma, Heinz, Gumprecht, Lars, Hajdu, Janos, Hampton, Christina Y., Hartmann, Andreas, Hartmann, Elisabeth, Hartmann, Robert, Hauser, Gunter, Hirsemann, Helmut, Holl, Peter, Kassemeyer, Stephan, Kimmel, Nils, Kiskinova, Maya, Liang, Mengning, Loh, Ne-Te Duane, Lomb, Lukas, Maia, Filipe R. N. C., Martin, Andrew V., Nass, Karol, Pedersoli, Emanuele, Reich, Christian, Rolles, Daniel, Rudek, Benedikt, Rudenko, Artem, Schlichting, Ilme, Schulz, Joachim, Seibert, Marvin, Seltzer, Virginie, Shoeman, Robert L., Sierra, Raymond G., Soltau, Heike, Starodub, Dmitri, Steinbrener, Jan, Stier, Gunter, Strueder, Lothar, Svenda, Martin, Ullrich, Joachim, Weidenspointner, Georg, White, Thomas A., Wunderer, Cornelia, and Ourmazd, Abbas

Abstract

Single-particle experiments using X-ray Free Electron Lasers produce more than 10(5) snapshots per hour, consisting of an admixture of blank shots (no particle intercepted), and exposures of one or more particles. Experimental data sets also often contain unintentional contamination with different species. We present an unsupervised method able to sort experimental snapshots without recourse to templates, specific noise models, or user-directed learning. The results show 90% agreement with manual classification.

Published
2011
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33. Radiation damage in protein serial femtosecond crystallography using an x-ray free-electron laser

Author

Lomb, Lukas, Barends, Thomas R. M., Kassemeyer, Stephan, Aquila, Andrew, Epp, Sascha W., Erk, Benjamin, Foucar, Lutz, Hartmann, Robert, Rudek, Benedikt, Rolles, Daniel, Rudenko, Artem, Shoeman, Robert L., Andreasson, Jakob, Bajt, Sasa, Barthelmess, Miriam, Barty, Anton, Bogan, Michael J., Bostedt, Christoph, Bozek, John D., Caleman, Carl, Coffee, Ryan, Coppola, Nicola, DePonte, Daniel P., Doak, R. Bruce, Ekeberg, Tomas, Fleckenstein, Holger, Fromme, Petra, Gebhardt, Maike, Graafsma, Heinz, Gumprecht, Lars, Hampton, Christina Y., Hartmann, Andreas, Hauser, Guenter, Hirsemann, Helmut, Holl, Peter, Holton, James M., Hunter, Mark S., Kabsch, Wolfgang, Kimmel, Nils, Kirian, Richard A., Liang, Mengning, Maia, Filipe R. N. C., Meinhart, Anton, Marchesini, Stefano, Martin, Andrew V., Nass, Karol, Reich, Christian, Schulz, Joachim, Seibert, M. Marvin, Sierra, Raymond, Soltau, Heike, Spence, John C. H., Steinbrener, Jan, Stellato, Francesco, Stern, Stephan, Timneanu, Nicusor, Wang, Xiaoyu, Weidenspointner, Georg, Weierstall, Uwe, White, Thomas A., Wunderer, Cornelia, Chapman, Henry N., Ullrich, Joachim, Strüder, Lothar, Schlichting, Ilme, Lomb, Lukas, Barends, Thomas R. M., Kassemeyer, Stephan, Aquila, Andrew, Epp, Sascha W., Erk, Benjamin, Foucar, Lutz, Hartmann, Robert, Rudek, Benedikt, Rolles, Daniel, Rudenko, Artem, Shoeman, Robert L., Andreasson, Jakob, Bajt, Sasa, Barthelmess, Miriam, Barty, Anton, Bogan, Michael J., Bostedt, Christoph, Bozek, John D., Caleman, Carl, Coffee, Ryan, Coppola, Nicola, DePonte, Daniel P., Doak, R. Bruce, Ekeberg, Tomas, Fleckenstein, Holger, Fromme, Petra, Gebhardt, Maike, Graafsma, Heinz, Gumprecht, Lars, Hampton, Christina Y., Hartmann, Andreas, Hauser, Guenter, Hirsemann, Helmut, Holl, Peter, Holton, James M., Hunter, Mark S., Kabsch, Wolfgang, Kimmel, Nils, Kirian, Richard A., Liang, Mengning, Maia, Filipe R. N. C., Meinhart, Anton, Marchesini, Stefano, Martin, Andrew V., Nass, Karol, Reich, Christian, Schulz, Joachim, Seibert, M. Marvin, Sierra, Raymond, Soltau, Heike, Spence, John C. H., Steinbrener, Jan, Stellato, Francesco, Stern, Stephan, Timneanu, Nicusor, Wang, Xiaoyu, Weidenspointner, Georg, Weierstall, Uwe, White, Thomas A., Wunderer, Cornelia, Chapman, Henry N., Ullrich, Joachim, Strüder, Lothar, and Schlichting, Ilme

Abstract

X-ray free-electron lasers deliver intense femtosecond pulses that promise to yield high resolution diffraction data of nanocrystals before the destruction of the sample by radiation damage. Diffraction intensities of lysozyme nanocrystals collected at the Linac Coherent Light Source using 2 keV photons were used for structure determination by molecular replacement and analyzed for radiation damage as a function of pulse length and fluence. Signatures of radiation damage are observed for pulses as short as 70 fs. Parametric scaling used in conventional crystallography does not account for the observed effects.

Published
2011
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34. X-Ray Diffraction from Isolated and Strongly Aligned Gas-Phase Molecules with a Free-Electron Laser

Author

Küpper, Jochen, primary, Stern, Stephan, additional, Holmegaard, Lotte, additional, Filsinger, Frank, additional, Rouzée, Arnaud, additional, Rudenko, Artem, additional, Johnsson, Per, additional, Martin, Andrew V., additional, Adolph, Marcus, additional, Aquila, Andrew, additional, Bajt, Saša, additional, Barty, Anton, additional, Bostedt, Christoph, additional, Bozek, John, additional, Caleman, Carl, additional, Coffee, Ryan, additional, Coppola, Nicola, additional, Delmas, Tjark, additional, Epp, Sascha, additional, Erk, Benjamin, additional, Foucar, Lutz, additional, Gorkhover, Tais, additional, Gumprecht, Lars, additional, Hartmann, Andreas, additional, Hartmann, Robert, additional, Hauser, Günter, additional, Holl, Peter, additional, Hömke, Andre, additional, Kimmel, Nils, additional, Krasniqi, Faton, additional, Kühnel, Kai-Uwe, additional, Maurer, Jochen, additional, Messerschmidt, Marc, additional, Moshammer, Robert, additional, Reich, Christian, additional, Rudek, Benedikt, additional, Santra, Robin, additional, Schlichting, Ilme, additional, Schmidt, Carlo, additional, Schorb, Sebastian, additional, Schulz, Joachim, additional, Soltau, Heike, additional, Spence, John C. H., additional, Starodub, Dmitri, additional, Strüder, Lothar, additional, Thøgersen, Jan, additional, Vrakking, Marc J. J., additional, Weidenspointner, Georg, additional, White, Thomas A., additional, Wunderer, Cornelia, additional, Meijer, Gerard, additional, Ullrich, Joachim, additional, Stapelfeldt, Henrik, additional, Rolles, Daniel, additional, and Chapman, Henry N., additional

Published
2014
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35. Toward unsupervised single-shot diffractive imaging of heterogeneous particles using X-ray free-electron lasers

Author

Park, Hyung Joo, primary, Loh, N. Duane, additional, Sierra, Raymond G., additional, Hampton, Christina Y., additional, Starodub, Dmitri, additional, Martin, Andrew V., additional, Barty, Anton, additional, Aquila, Andrew, additional, Schulz, Joachim, additional, Steinbrener, Jan, additional, Shoeman, Robert L., additional, Lomb, Lukas, additional, Kassemeyer, Stephan, additional, Bostedt, Christoph, additional, Bozek, John, additional, Epp, Sascha W., additional, Erk, Benjamin, additional, Hartmann, Robert, additional, Rolles, Daniel, additional, Rudenko, Artem, additional, Rudek, Benedikt, additional, Foucar, Lutz, additional, Kimmel, Nils, additional, Weidenspointner, Georg, additional, Hauser, Guenter, additional, Holl, Peter, additional, Pedersoli, Emanuele, additional, Liang, Mengning, additional, Hunter, Mark S., additional, Gumprecht, Lars, additional, Coppola, Nicola, additional, Wunderer, Cornelia, additional, Graafsma, Heinz, additional, Maia, Filipe R.N.C., additional, Ekeberg, Tomas, additional, Hantke, Max, additional, Fleckenstein, Holger, additional, Hirsemann, Helmut, additional, Nass, Karol, additional, Tobias, Herbert J., additional, Farquar, George R., additional, Benner, W. Henry, additional, Hau-Riege, Stefan, additional, Reich, Christian, additional, Hartmann, Andreas, additional, Soltau, Heike, additional, Marchesini, Stefano, additional, Bajt, Sasa, additional, Barthelmess, Miriam, additional, Strueder, Lothar, additional, Ullrich, Joachim, additional, Bucksbaum, Philip, additional, Frank, Matthias, additional, Schlichting, Ilme, additional, Chapman, Henry N., additional, Bogan, Michael J., additional, and Elser, Veit, additional

Published
2013
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36. Sensing the wavefront of x-ray free-electron lasers using aerosol spheres

Author

Loh, N. Duane, primary, Starodub, Dmitri, additional, Lomb, Lukas, additional, Hampton, Christina Y., additional, Martin, Andrew V., additional, Sierra, Raymond G., additional, Barty, Anton, additional, Aquila, Andrew, additional, Schulz, Joachim, additional, Steinbrener, Jan, additional, Shoeman, Robert L., additional, Kassemeyer, Stephan, additional, Bostedt, Christoph, additional, Bozek, John, additional, Epp, Sascha W., additional, Erk, Benjamin, additional, Hartmann, Robert, additional, Rolles, Daniel, additional, Rudenko, Artem, additional, Rudek, Benedikt, additional, Foucar, Lutz, additional, Kimmel, Nils, additional, Weidenspointner, Georg, additional, Hauser, Guenter, additional, Holl, Peter, additional, Pedersoli, Emanuele, additional, Liang, Mengning, additional, Hunter, Mark S., additional, Gumprecht, Lars, additional, Coppola, Nicola, additional, Wunderer, Cornelia, additional, Graafsma, Heinz, additional, Maia, Filipe R.N.C., additional, Ekeberg, Tomas, additional, Hantke, Max, additional, Fleckenstein, Holger, additional, Hirsemann, Helmut, additional, Nass, Karol, additional, White, Thomas A., additional, Tobias, Herbert J., additional, Farquar, George R., additional, Benner, W. Henry, additional, Hau-Riege, Stefan, additional, Reich, Christian, additional, Hartmann, Andreas, additional, Soltau, Heike, additional, Marchesini, Stefano, additional, Bajt, Sasa, additional, Barthelmess, Miriam, additional, Strueder, Lothar, additional, Ullrich, Joachim, additional, Bucksbaum, Philip, additional, Frank, Matthias, additional, Schlichting, Ilme, additional, Chapman, Henry N., additional, and Bogan, Michael J., additional

Published
2013
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37. Ultra-efficient ionization of heavy atoms by intense X-ray free-electron laser pulses

Author

Rudek, Benedikt, primary, Son, Sang-Kil, additional, Foucar, Lutz, additional, Epp, Sascha W., additional, Erk, Benjamin, additional, Hartmann, Robert, additional, Adolph, Marcus, additional, Andritschke, Robert, additional, Aquila, Andrew, additional, Berrah, Nora, additional, Bostedt, Christoph, additional, Bozek, John, additional, Coppola, Nicola, additional, Filsinger, Frank, additional, Gorke, Hubert, additional, Gorkhover, Tais, additional, Graafsma, Heinz, additional, Gumprecht, Lars, additional, Hartmann, Andreas, additional, Hauser, Günter, additional, Herrmann, Sven, additional, Hirsemann, Helmut, additional, Holl, Peter, additional, Hömke, André, additional, Journel, Loic, additional, Kaiser, Christian, additional, Kimmel, Nils, additional, Krasniqi, Faton, additional, Kühnel, Kai-Uwe, additional, Matysek, Michael, additional, Messerschmidt, Marc, additional, Miesner, Danilo, additional, Möller, Thomas, additional, Moshammer, Robert, additional, Nagaya, Kiyonobu, additional, Nilsson, Björn, additional, Potdevin, Guillaume, additional, Pietschner, Daniel, additional, Reich, Christian, additional, Rupp, Daniela, additional, Schaller, Gerhard, additional, Schlichting, Ilme, additional, Schmidt, Carlo, additional, Schopper, Florian, additional, Schorb, Sebastian, additional, Schröter, Claus-Dieter, additional, Schulz, Joachim, additional, Simon, Marc, additional, Soltau, Heike, additional, Strüder, Lothar, additional, Ueda, Kiyoshi, additional, Weidenspointner, Georg, additional, Santra, Robin, additional, Ullrich, Joachim, additional, Rudenko, Artem, additional, and Rolles, Daniel, additional

Published
2012
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38. Profiling structured beams using injected aerosols

Author

Loh, N. D., primary, Starodub, Dmitri, additional, Lomb, Lukas, additional, Hampton, Christina Y., additional, Martin, Andrew V., additional, Sierra, Raymond G., additional, Barty, Anton, additional, Aquila, Andrew, additional, Schulz, Joachim, additional, Steinbrener, Jan, additional, Shoeman, Robert L., additional, Kassemeyer, Stephan, additional, Bostedt, Christoph, additional, Bozek, John, additional, Epp, Sascha W., additional, Erk, Benjamin, additional, Hartmann, Robert, additional, Rolles, Daniel, additional, Rudenko, Artem, additional, Rudek, Benedikt, additional, Foucar, Lutz, additional, Kimmel, Nils, additional, Weidenspointner, Georg, additional, Hauser, Günther, additional, Holl, Peter, additional, Pedersoli, Emanuele, additional, Liang, MengNing, additional, Hunter, Mark S., additional, Gumprecht, Lars, additional, Coppola, Nicola, additional, Wunderer, Cornelia, additional, Graafsman, Heinz, additional, Maia, Filipe R. N. C., additional, Ekeberg, Tomas, additional, Hantke, Max, additional, Fleckenstein, Holger, additional, Hirsemann, Helmut, additional, Nass, Karol, additional, White, Thomas A., additional, Tobias, Herbert J., additional, Farquar, George R., additional, Benner, W. Henry, additional, Hau-Riege, Stefan, additional, Reich, Christian, additional, Hartmann, Andreas, additional, Soltau, Heike, additional, Marchesini, Stefano, additional, Bajt, Sasa, additional, Barthelmess, Miriam, additional, Strueder, Lothar, additional, Ullrich, Joachim, additional, Bucksbaum, Philip, additional, Hodgson, Keith O., additional, Frank, Mathias, additional, Schlichting, Ilme, additional, Chapman, Henry N., additional, and Bogan, Michael J., additional

Published
2012
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39. Femtosecond free-electron laser x-ray diffraction data sets for algorithm development

Author

Kassemeyer, Stephan, primary, Steinbrener, Jan, additional, Lomb, Lukas, additional, Hartmann, Elisabeth, additional, Aquila, Andrew, additional, Barty, Anton, additional, Martin, Andrew V., additional, Hampton, Christina Y., additional, Bajt, Saša, additional, Barthelmess, Miriam, additional, Barends, Thomas R.M., additional, Bostedt, Christoph, additional, Bott, Mario, additional, Bozek, John D., additional, Coppola, Nicola, additional, Cryle, Max, additional, DePonte, Daniel P., additional, Doak, R. Bruce, additional, Epp, Sascha W., additional, Erk, Benjamin, additional, Fleckenstein, Holger, additional, Foucar, Lutz, additional, Graafsma, Heinz, additional, Gumprecht, Lars, additional, Hartmann, Andreas, additional, Hartmann, Robert, additional, Hauser, Günter, additional, Hirsemann, Helmut, additional, Hömke, André, additional, Holl, Peter, additional, Jönsson, Olof, additional, Kimmel, Nils, additional, Krasniqi, Faton, additional, Liang, Mengning, additional, Maia, Filipe R.N.C., additional, Marchesini, Stefano, additional, Nass, Karol, additional, Reich, Christian, additional, Rolles, Daniel, additional, Rudek, Benedikt, additional, Rudenko, Artem, additional, Schmidt, Carlo, additional, Schulz, Joachim, additional, Shoeman, Robert L., additional, Sierra, Raymond G., additional, Soltau, Heike, additional, Spence, John C. H., additional, Starodub, Dmitri, additional, Stellato, Francesco, additional, Stern, Stephan, additional, Stier, Gunter, additional, Svenda, Martin, additional, Weidenspointner, Georg, additional, Weierstall, Uwe, additional, White, Thomas A., additional, Wunderer, Cornelia, additional, Frank, Matthias, additional, Chapman, Henry N., additional, Ullrich, Joachim, additional, Strüder, Lothar, additional, Bogan, Michael J., additional, and Schlichting, Ilme, additional

Published
2012
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40. In vivo protein crystallization opens new routes in structural biology

Author

Koopmann, Rudolf, primary, Cupelli, Karolina, additional, Redecke, Lars, additional, Nass, Karol, additional, DePonte, Daniel P, additional, White, Thomas A, additional, Stellato, Francesco, additional, Rehders, Dirk, additional, Liang, Mengning, additional, Andreasson, Jakob, additional, Aquila, Andrew, additional, Bajt, Sasa, additional, Barthelmess, Miriam, additional, Barty, Anton, additional, Bogan, Michael J, additional, Bostedt, Christoph, additional, Boutet, Sébastien, additional, Bozek, John D, additional, Caleman, Carl, additional, Coppola, Nicola, additional, Davidsson, Jan, additional, Doak, R Bruce, additional, Ekeberg, Tomas, additional, Epp, Sascha W, additional, Erk, Benjamin, additional, Fleckenstein, Holger, additional, Foucar, Lutz, additional, Graafsma, Heinz, additional, Gumprecht, Lars, additional, Hajdu, Janos, additional, Hampton, Christina Y, additional, Hartmann, Andreas, additional, Hartmann, Robert, additional, Hauser, Günter, additional, Hirsemann, Helmut, additional, Holl, Peter, additional, Hunter, Mark S, additional, Kassemeyer, Stephan, additional, Kirian, Richard A, additional, Lomb, Lukas, additional, Maia, Filipe R N C, additional, Kimmel, Nils, additional, Martin, Andrew V, additional, Messerschmidt, Marc, additional, Reich, Christian, additional, Rolles, Daniel, additional, Rudek, Benedikt, additional, Rudenko, Artem, additional, Schlichting, Ilme, additional, Schulz, Joachim, additional, Seibert, M Marvin, additional, Shoeman, Robert L, additional, Sierra, Raymond G, additional, Soltau, Heike, additional, Stern, Stephan, additional, Strüder, Lothar, additional, Timneanu, Nicusor, additional, Ullrich, Joachim, additional, Wang, Xiaoyu, additional, Weidenspointner, Georg, additional, Weierstall, Uwe, additional, Williams, Garth J, additional, Wunderer, Cornelia B, additional, Fromme, Petra, additional, Spence, John C H, additional, Stehle, Thilo, additional, Chapman, Henry N, additional, Betzel, Christian, additional, and Duszenko, Michael, additional

Published
2012
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41. Lipidic phase membrane protein serial femtosecond crystallography

Author

Johansson, Linda C, primary, Arnlund, David, additional, White, Thomas A, additional, Katona, Gergely, additional, DePonte, Daniel P, additional, Weierstall, Uwe, additional, Doak, R Bruce, additional, Shoeman, Robert L, additional, Lomb, Lukas, additional, Malmerberg, Erik, additional, Davidsson, Jan, additional, Nass, Karol, additional, Liang, Mengning, additional, Andreasson, Jakob, additional, Aquila, Andrew, additional, Bajt, Saša, additional, Barthelmess, Miriam, additional, Barty, Anton, additional, Bogan, Michael J, additional, Bostedt, Christoph, additional, Bozek, John D, additional, Caleman, Carl, additional, Coffee, Ryan, additional, Coppola, Nicola, additional, Ekeberg, Tomas, additional, Epp, Sascha W, additional, Erk, Benjamin, additional, Fleckenstein, Holger, additional, Foucar, Lutz, additional, Graafsma, Heinz, additional, Gumprecht, Lars, additional, Hajdu, Janos, additional, Hampton, Christina Y, additional, Hartmann, Robert, additional, Hartmann, Andreas, additional, Hauser, Günter, additional, Hirsemann, Helmut, additional, Holl, Peter, additional, Hunter, Mark S, additional, Kassemeyer, Stephan, additional, Kimmel, Nils, additional, Kirian, Richard A, additional, Maia, Filipe R N C, additional, Marchesini, Stefano, additional, Martin, Andrew V, additional, Reich, Christian, additional, Rolles, Daniel, additional, Rudek, Benedikt, additional, Rudenko, Artem, additional, Schlichting, Ilme, additional, Schulz, Joachim, additional, Seibert, M Marvin, additional, Sierra, Raymond G, additional, Soltau, Heike, additional, Starodub, Dmitri, additional, Stellato, Francesco, additional, Stern, Stephan, additional, Strüder, Lothar, additional, Timneanu, Nicusor, additional, Ullrich, Joachim, additional, Wahlgren, Weixiao Y, additional, Wang, Xiaoyu, additional, Weidenspointner, Georg, additional, Wunderer, Cornelia, additional, Fromme, Petra, additional, Chapman, Henry N, additional, Spence, John C H, additional, and Neutze, Richard, additional

Published
2012
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42. Radiation damage in protein serial femtosecond crystallography using an x-ray free-electron laser

Author

Lomb, Lukas, primary, Barends, Thomas R. M., additional, Kassemeyer, Stephan, additional, Aquila, Andrew, additional, Epp, Sascha W., additional, Erk, Benjamin, additional, Foucar, Lutz, additional, Hartmann, Robert, additional, Rudek, Benedikt, additional, Rolles, Daniel, additional, Rudenko, Artem, additional, Shoeman, Robert L., additional, Andreasson, Jakob, additional, Bajt, Sasa, additional, Barthelmess, Miriam, additional, Barty, Anton, additional, Bogan, Michael J., additional, Bostedt, Christoph, additional, Bozek, John D., additional, Caleman, Carl, additional, Coffee, Ryan, additional, Coppola, Nicola, additional, DePonte, Daniel P., additional, Doak, R. Bruce, additional, Ekeberg, Tomas, additional, Fleckenstein, Holger, additional, Fromme, Petra, additional, Gebhardt, Maike, additional, Graafsma, Heinz, additional, Gumprecht, Lars, additional, Hampton, Christina Y., additional, Hartmann, Andreas, additional, Hauser, Günter, additional, Hirsemann, Helmut, additional, Holl, Peter, additional, Holton, James M., additional, Hunter, Mark S., additional, Kabsch, Wolfgang, additional, Kimmel, Nils, additional, Kirian, Richard A., additional, Liang, Mengning, additional, Maia, Filipe R. N. C., additional, Meinhart, Anton, additional, Marchesini, Stefano, additional, Martin, Andrew V., additional, Nass, Karol, additional, Reich, Christian, additional, Schulz, Joachim, additional, Seibert, M. Marvin, additional, Sierra, Raymond, additional, Soltau, Heike, additional, Spence, John C. H., additional, Steinbrener, Jan, additional, Stellato, Francesco, additional, Stern, Stephan, additional, Timneanu, Nicusor, additional, Wang, Xiaoyu, additional, Weidenspointner, Georg, additional, Weierstall, Uwe, additional, White, Thomas A., additional, Wunderer, Cornelia, additional, Chapman, Henry N., additional, Ullrich, Joachim, additional, Strüder, Lothar, additional, and Schlichting, Ilme, additional

Published
2011
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43. Self-terminating diffraction gates femtosecond X-ray nanocrystallography measurements

Author

Barty, Anton, primary, Caleman, Carl, additional, Aquila, Andrew, additional, Timneanu, Nicusor, additional, Lomb, Lukas, additional, White, Thomas A., additional, Andreasson, Jakob, additional, Arnlund, David, additional, Bajt, Saša, additional, Barends, Thomas R. M., additional, Barthelmess, Miriam, additional, Bogan, Michael J., additional, Bostedt, Christoph, additional, Bozek, John D., additional, Coffee, Ryan, additional, Coppola, Nicola, additional, Davidsson, Jan, additional, DePonte, Daniel P., additional, Doak, R. Bruce, additional, Ekeberg, Tomas, additional, Elser, Veit, additional, Epp, Sascha W., additional, Erk, Benjamin, additional, Fleckenstein, Holger, additional, Foucar, Lutz, additional, Fromme, Petra, additional, Graafsma, Heinz, additional, Gumprecht, Lars, additional, Hajdu, Janos, additional, Hampton, Christina Y., additional, Hartmann, Robert, additional, Hartmann, Andreas, additional, Hauser, Günter, additional, Hirsemann, Helmut, additional, Holl, Peter, additional, Hunter, Mark S., additional, Johansson, Linda, additional, Kassemeyer, Stephan, additional, Kimmel, Nils, additional, Kirian, Richard A., additional, Liang, Mengning, additional, Maia, Filipe R. N. C., additional, Malmerberg, Erik, additional, Marchesini, Stefano, additional, Martin, Andrew V., additional, Nass, Karol, additional, Neutze, Richard, additional, Reich, Christian, additional, Rolles, Daniel, additional, Rudek, Benedikt, additional, Rudenko, Artem, additional, Scott, Howard, additional, Schlichting, Ilme, additional, Schulz, Joachim, additional, Seibert, M. Marvin, additional, Shoeman, Robert L., additional, Sierra, Raymond G., additional, Soltau, Heike, additional, Spence, John C. H., additional, Stellato, Francesco, additional, Stern, Stephan, additional, Strüder, Lothar, additional, Ullrich, Joachim, additional, Wang, X., additional, Weidenspointner, Georg, additional, Weierstall, Uwe, additional, Wunderer, Cornelia B., additional, and Chapman, Henry N., additional

Published
2011
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44. Unsupervised classification of single-particle X-ray diffraction snapshots by spectral clustering

Author

Yoon, Chun Hong, primary, Schwander, Peter, additional, Abergel, Chantal, additional, Andersson, Inger, additional, Andreasson, Jakob, additional, Aquila, Andrew, additional, Bajt, Saša, additional, Barthelmess, Miriam, additional, Barty, Anton, additional, Bogan, Michael J., additional, Bostedt, Christoph, additional, Bozek, John, additional, Chapman, Henry N., additional, Claverie, Jean-Michel, additional, Coppola, Nicola, additional, DePonte, Daniel P., additional, Ekeberg, Tomas, additional, Epp, Sascha W., additional, Erk, Benjamin, additional, Fleckenstein, Holger, additional, Foucar, Lutz, additional, Graafsma, Heinz, additional, Gumprecht, Lars, additional, Hajdu, Janos, additional, Hampton, Christina Y., additional, Hartmann, Andreas, additional, Hartmann, Elisabeth, additional, Hartmann, Robert, additional, Hauser, Gunter, additional, Hirsemann, Helmut, additional, Holl, Peter, additional, Kassemeyer, Stephan, additional, Kimmel, Nils, additional, Kiskinova, Maya, additional, Liang, Mengning, additional, Loh, Ne-Te Duane, additional, Lomb, Lukas, additional, Maia, Filipe R. N. C., additional, Martin, Andrew V., additional, Nass, Karol, additional, Pedersoli, Emanuele, additional, Reich, Christian, additional, Rolles, Daniel, additional, Rudek, Benedikt, additional, Rudenko, Artem, additional, Schlichting, Ilme, additional, Schulz, Joachim, additional, Seibert, Marvin, additional, Seltzer, Virginie, additional, Shoeman, Robert L., additional, Sierra, Raymond G., additional, Soltau, Heike, additional, Starodub, Dmitri, additional, Steinbrener, Jan, additional, Stier, Gunter, additional, Strüder, Lothar, additional, Svenda, Martin, additional, Ullrich, Joachim, additional, Weidenspointner, Georg, additional, White, Thomas A., additional, Wunderer, Cornelia, additional, and Ourmazd, Abbas, additional

Published
2011
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45. Large format imaging detectors for x-ray free-electron-lasers

Author

Hartmann, Robert, primary, Epp, Sascha, additional, Gorke, Hubert, additional, Hartmann, Andreas, additional, Hauser, Günther, additional, Herrmann, Sven, additional, Holl, Peter, additional, Kimmel, Nils, additional, Meidinger, Norbert, additional, Reich, Christian, additional, Rolles, Daniel, additional, Soltau, Heike, additional, Strüder, Lothar, additional, Ullrich, Joachim, additional, and Weidenspointner, Georg, additional

Published
2011
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46. Single particle imaging with soft x-rays at the Linac Coherent Light Source

Author

Martin, Andrew V., primary, Andreasson, Jakob, additional, Aquila, Andrew, additional, Bajt, Saša, additional, Barends, Thomas R. M., additional, Barthelmess, Miriam, additional, Barty, Anton, additional, Benner, W. Henry, additional, Bostedt, Christoph, additional, Bozek, John D., additional, Bucksbaum, Phillip, additional, Caleman, Carl, additional, Coppola, Nicola, additional, DePonte, Daniel P., additional, Ekeberg, Tomas, additional, Epp, Sascha W., additional, Erk, Benjamin, additional, Farquar, George R., additional, Fleckenstein, Holger, additional, Foucar, Lutz, additional, Frank, Matthias, additional, Gumprecht, Lars, additional, Hampton, Christina Y., additional, Hantke, Max, additional, Hartmann, Andreas, additional, Hartmann, Elisabeth, additional, Hartmann, Robert, additional, Hau-Riege, Stephan P., additional, Hauser, Günther, additional, Holl, Peter, additional, Hoemke, André, additional, Jönsson, Olof, additional, Kassemeyer, Stephan, additional, Kimmel, Nils, additional, Kiskinova, Maya, additional, Krasniqi, Faton, additional, Krzywinski, Jacek, additional, Liang, Mengning, additional, Loh, Ne-Te Duane, additional, Lomb, Lukas, additional, Maia, Filipe R. N. C., additional, Marchesini, Stefano, additional, Messerschmidt, Marc, additional, Nass, Karol, additional, Odic, Duško, additional, Pedersoli, Emanuele, additional, Reich, Christian, additional, Rolles, Daniel, additional, Rudek, Benedikt, additional, Rudenko, Artem, additional, Schmidt, Carlo, additional, Schultz, Joachim, additional, Seibert, M. Marvin, additional, Shoeman, Robert L., additional, Sierra, Raymond G., additional, Soltau, Heike, additional, Starodub, Dmitri, additional, Steinbrener, Jan, additional, Stellato, Francesco, additional, Strüder, Lothar, additional, Svenda, Martin, additional, Tobias, Herbert, additional, Ullrich, Joachim, additional, Weidenspointner, Georg, additional, Westphal, Daniel, additional, White, Thomas A., additional, Williams, Garth, additional, Hajdu, Janos, additional, Schlichting, Ilme, additional, Bogan, Michael J., additional, and Chapman, Henry N., additional

Published
2011
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48. CCD detector development for the eROSITA space telescope

Author

Meidinger, Norbert, primary, Andritschke, Robert, additional, Assmann, Walter, additional, Ebermayer, Stefanie, additional, Elbs, Johannes, additional, Halker, Olaf, additional, Heindl, Emanuel, additional, Herrmann, Sven, additional, Kimmel, Nils, additional, Pietschner, Daniel, additional, Reiffers, Jonas, additional, Reinhardt, Sabine, additional, Schachner, Gabriele, additional, Struder, Lothar, additional, and Weidenspointner, Georg, additional

Published
2010
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49. The Wide Field Imager of the European X-Ray Observatory

Author

Lechner, Peter, primary, Andricek, Ladislav, additional, Heinzinger, Klaus, additional, Herrmann, Sven, additional, Kimmel, Nils, additional, Lauf, Thomas, additional, Lutz, Gerhard, additional, Richter, Rainer H., additional, Porro, Matteo, additional, Schaller, Gerhard, additional, Schnecke, Martina, additional, Schopper, Florian, additional, Soltau, Heike, additional, Struder, Lothar, additional, Treis, Johannes, additional, Wufel, Stefan, additional, and Chen, Zhang, additional

Published
2006
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50. CCD-detector development for the DUO and the ROSITA missions

Author

Meidinger, Norbert, primary, Bonerz, Stefan, additional, Englhauser, Jakob, additional, Hasinger, Guenther, additional, Hartmann, Robert, additional, Herrmann, Sven, additional, Holl, Peter, additional, Kettenring, Guenther, additional, Kimmel, Nils, additional, Lutz, Gerhard, additional, Pfeffermann, Elmar, additional, Predehl, Peter, additional, Soltau, Heike, additional, and Strueder, Lothar, additional

Published
2004
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