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28 results on '"Kim Kley Valladares-Diestra"'

1. Emerging contaminants bioremediation by enzyme and nanozyme-based processes – A review

Author

Gustavo Amaro Bittencourt, Luciana Porto de Souza Vandenberghe, Walter José Martínez-Burgos, Kim Kley Valladares-Diestra, Ariane Fátima Murawski de Mello, Bruna Leal Maske, Satinder Kaur Brar, Sunita Varjani, Gilberto Vinicius de Melo Pereira, and Carlos Ricardo Soccol

Subjects
Biotechnology, Environmental Biotechnology, Environmental science, Pollution, Science
Abstract

Summary: Due to their widespread occurrence and the inadequate removal efficiencies by conventional wastewater treatment plants, emerging contaminants (ECs) have recently become an issue of great concern. Current ongoing studies have focused on different physical, chemical, and biological methods as strategies to avoid exposing ecosystems to significant long-term risks. Among the different proposed technologies, the enzyme-based processes rise as green biocatalysts with higher efficiency yields and lower generation of toxic by-products. Oxidoreductases and hydrolases are among the most prominent enzymes applied for bioremediation processes. The present work overviews the state of the art of recent advances in enzymatic processes during wastewater treatment of EC, focusing on recent innovations in terms of applied immobilization techniques, genetic engineering tools, and the advent of nanozymes. Future trends in the enzymes immobilization techniques for EC removal were highlighted. Research gaps and recommendations on methods and utility of enzymatic treatment incorporation in conventional wastewater treatment plants were also discussed.

Published
2023
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2. The Potential of Xylooligosaccharides as Prebiotics and Their Sustainable Production from Agro-Industrial by-Products

Author

Kim Kley Valladares-Diestra, Luciana Porto de Souza Vandenberghe, Sabrina Vieira, Luis Daniel Goyzueta-Mamani, Patricia Beatriz Gruening de Mattos, Maria Clara Manzoki, Vanete Thomaz Soccol, and Carlos Ricardo Soccol

Subjects
xylooligosaccharides, prebiotics, lignocellulosic biomass, agro-industrial by-products, Chemical technology, TP1-1185
Abstract

In recent years, concerns about a good-quality diet have increased. Food supplements such as prebiotics have great nutritional and health benefits. Within the diverse range of prebiotics, xylooligosaccharides (XOs) show high potential, presenting exceptional properties for the prevention of systemic disorders. XOs can be found in different natural sources; however, their production is limited. Lignocellulosic biomasses present a high potential as a source of raw material for the production of XOs, making the agro-industrial by-products the perfect candidates for production on an industrial scale. However, these biomasses require the application of physicochemical pretreatments to obtain XOs. Different pretreatment methodologies are discussed in terms of increasing the production of XOs and limiting the coproduction of toxic compounds. The advance in new technologies for XOs production could decrease their real cost (USD 25–50/kg) on an industrial scale and would increase the volume of market transactions in the prebiotic sector (USD 4.5 billion). In this sense, new patents and innovations are being strategically developed to expand the use of XOs as daily prebiotics.

Published
2023
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3. Second Generation Bioethanol Production from Soybean Hulls Pretreated with Imidazole as a New Solvent

Author

Verônica Sayuri Nishida, Adenise Lorenci Woiciechowski, Kim Kley Valladares-Diestra, Luis Alberto Zevallos Torres, Luciana Porto de Souza Vandenberghe, Arion Zandoná Filho, and Carlos Ricardo Soccol

Subjects
soybean hull, bioethanol, imidazole, enzymatic hydrolysis, pretreatment, Fermentation industries. Beverages. Alcohol, TP500-660
Abstract

Soybean hulls (SH) are the main industrial waste from soybean processing, representing 5–8% of the whole grain. Imidazole was employed for the hydrothermal pretreatment of SH and further bioethanol production. Different pretreatment temperatures (120 and 180 °C) and times (1 and 3 h) were tested. Lignin removal and glucose yield were significantly influenced by temperature. After 48 h of enzymatic hydrolysis of imidazole-treated SH (120 °C, 1 h), 32.7 g/L of glucose and 9.4 g/L of xylose were obtained. A maximum bioethanol yield of 78.9% was reached after 12 h of fermentation by Saccharomyces cerevisiae using SH enzymatic hydrolysate. Imidazole appears to be a potential alternative to pretreat lignocellulosic wastes such as SH for the production of second-generation biofuels and other biomolecules.

Published
2023
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7. Polyhydroxybutyrate production by Cupriavidus necator in a corn biorefinery concept

Author

Ariane Fátima Murawski de Mello, Luciana Porto de Souza Vandenberghe, Clara Matte Borges Machado, Kim Kley Valladares-Diestra, Júlio César de Carvalho, and Carlos Ricardo Soccol

Subjects
Environmental Engineering, Renewable Energy, Sustainability and the Environment, Bioengineering, General Medicine, Waste Management and Disposal
Abstract

The high costs of bioplastics' production may hinder their commercialization. Development of new processes with high yields and in biorefineries can enhance diffusion of these materials. This work evaluated the production of polyhydroxybutyrate (PHB) from the combination of milled corn starchy fraction hydrolysate and crude glycerol as substrates by the strain Cupriavidus necator LPB 1421. After optimization steps, maximum accumulation of 62 % of PHB was obtained, which represents 11.64 g.L

Published
2022

9. Oilseed Enzymatic Pretreatment for Efficient Oil Recovery in Biodiesel Production Industry: a Review

Author

Carlos Ricardo Soccol, Kim Kley Valladares-Diestra, and Luciana Porto de Souza Vandenberghe

Subjects
0106 biological sciences, Pollutant, Biodiesel, Rapeseed, Renewable Energy, Sustainability and the Environment, 020209 energy, Extraction (chemistry), food and beverages, 02 engineering and technology, Pulp and paper industry, complex mixtures, 01 natural sciences, Environmentally friendly, Hexane, chemistry.chemical_compound, Diesel fuel, chemistry, 010608 biotechnology, Biodiesel production, 0202 electrical engineering, electronic engineering, information engineering, Environmental science, Agronomy and Crop Science, Energy (miscellaneous)
Abstract

Biodiesel is an alternative to fossil diesel, non-toxic, and less pollutant. The production of biodiesel occurs with the use of oils, which are extracted from oleaginous seeds such as soybean, rapeseed, and palm fruit. The extraction of oils from oilseeds is one bottleneck of biodiesel production impacting on significant processes’ losses and productivity. This review analyzes different technologies to improve the oil extraction focusing on the enzyme-assisted aqueous extraction (EAAE) methods. EAAE is an environmentally friendly technology that takes advantage of the degradation efficiency of the enzymes, in this case hemicellulases preparations, which specifically degrade different structures that are present in vegetable cell walls to improve oil extraction from oilseeds. The enzymes used in this process are industrially produced and are highly efficient, but with high costs. The use of agro-industrial subproducts or the improvement of the enzymes’ producing strains could be an interesting solution for viable enzymatic preparations’ production to attend the biodiesel industries’ demand. EAAE treatment also shows great potential in techno-economic analysis allowing a faster recovery of profits and at a smaller production scale than the hexane extraction methodology.

Published
2020

10. Enzymatic bioremediation

Author

Luciana Porto de Souza Vandenberghe, Nelson Libardi Junior, Kim Kley Valladares-Diestra, Susan Grace Karp, Joyce Gueiros Wanderley Siqueira, Cristine Rodrigues, and Carlos Ricardo Soccol

Published
2022

12. Contributors

Author

Elijah Adegoke Adebayo, Komal Agrawal, Micheal Bukola Alao, Vandita Anand, Somdipta Bagchi, Sanchayita Basu, Pakiza Begum, Manaswini Behera, Navneeta Bharadvaja, Arunima Bhattacharjee, Joorie Bhattacharya, Gayatri Brahmandam, Nikita Chakraborty, Sourish Chakraborty, Dixita Chettri, Mohita Chugh, Praveen Dahiya, Subhasish Dutta, Vivek Kumar Gaur, Debojit Ghosh, Mrinmoy Ghosh, Sougata Ghosh, Umesh Goutam, Kumari Guddi, Raja Husain, Touseef Hussain, Nelson Libardi Junior, Susan Grace Karp, Priya Khadgawat, N.A. Khan, Ashutosh Kumar, Deepak Kumar, Lakhan Kumar, Sayan Laha, Arunima Lahiri, Natesan Manickam, Soma Nag, Rahul Nitnavare, Anjana Pandey, Sonika Pandey, Soumya Pandit, Bhisma Kumar Patel, Monika Patel, Bhumika Rajoria, Cristine Rodrigues, Arpita Roy, Rahul Saha, Angana Sarkar, Aveepsa Sengupta, Bhaskar Sharma, Nahid Masood Siddiqui, Neha Singh, Pragati Singh, Joyce Gueiros Wanderley Siqueira, Carlos Ricardo Soccol, Abhinav Suresh, Jolly Thomas, Varsha Tripathi, Kim Kley Valladares-Diestra, Luciana Porto de Souza Vandenberghe, Anil Kumar Verma, Pradeep Verma, Nitin Vikram, Thomas J. Webster, and Garima Yadav

Published
2022

14. Contributors

Author

Salma Aathika, Azhan Ahmad, D. Mubarak Ali, V. Amirthavalli, M. Aniskumar, Sameena Anjum, Renu Bala, E. Baldev, Debraj Bhattacharyya, Difran Nobel Bistara, Saptaswa Biswas, Gustavo Amaro Bittencourt, Ipsita Chakravarty, Angana Chaudhuri, Elsa Cherian, S. Chozhavendhan, Pratyush Kumar Das, Bidyut Prava Das, Sovik Das, Patitapaban Dash, Thiruselvi Devaraj, Fifi Khoirul Fitriyah, M.M. Ghangrekar, Rajat Giri, Marttin Paulraj Gundupalli, Sathish Paulraj Gundupalli, Goutam Kishore Gupta, Baskar Gurunathan, Mani Jayakumar, T. Jayasree Joshi, Nelson Libardi Junior, Luiz Alberto Junior Letti, G. Kalavathy, Ramani Kandasamy, Susan Grace Karp, G. Karthigadevi, Rupam Kataki, Manika D. Kataki, Santosh Kumar, Huei Yeong Lim, M. Grace Lydia Phoebe, Hilman Ibnu Mahdi, Sachin A. Mandavgane, Yuvarani Mani, Mpumelelo Thomas Matsena, Rahulkumar Maurya, Kaustubha Mohanty, Monoj Kumar Mondal, Ariane Fátima Murawski de Mello, Sanjana Narayanan, Nurfadhila Nasya binti Ramlee, Evans Martin Nkhalambayausi Chirwa, Mamta Pal, Shanmugam Palaniyandi, Radha Panjanathan, R. Praveenkumar, Mahendra Ram, E. Raja Sathendra, R. Sathya, P. Senthil Kumar, B. Senthil Rathi, Rakesh Kumar Sharma, Carlos Ricardo Soccol, Anne Sahithi Somavarapu Thomas, Sivanesan Subramanian, Achmad Syafiuddin, N. Thajuddin, Kim Kley Valladares-Diestra, Luciana Porto de Souza Vandenberghe, C.V. Vidhya, V. Vinoth Kumar, Nur Izyan Wan Azelee, Anita R. Warrier, Suzana Yusup, Zainul Akmar Zakaria, and Abdul Hakim Zakkiy Fasya

Published
2022

16. Contributors

Author

Gustavo Amaro Bittencourt, Xinhui Bao, Maria Giovana Binder Pagnoncelli, Yahui Bo, Pengfei Cheng, Ruirui Chu, Chicaiza-Ortiz Cristhian, Yanjun Dai, Júlio César de Carvalho, Lingkan Ding, Weiliang Dong, Yanzhang Feng, Chenjun He, Bo Hu, Min Jiang, Luiz Alberto Junior Letti, Xihui Kang, Susan Grace Karp, Kim Kley Valladares-Diestra, Jonathan T.E. Lee, Dongyi Li, Jingyi Li, Wangliang Li, Yang Li, Nelson Libardi Junior, Jun Wei Lim, Richen Lin, Haojie Liu, Kai-Chee Loh, Adenise Lorenci Woiciechowski, Yandu Lu, Liwen Luo, Tao Luo, Ariane Fátima Murawski de Mello, Jerry D Murphy, Nicholas Cheuk Him Ng, Qigui Niu, Luciana Porto de Souza Vandenberghe, Xiujuan Qian, Cristine Rodrigues, Roger Ruan, Zulma Sarmiento Vásquez, Fei Shen, Zhiqiang Shi, Carlos Ricardo Soccol, Liuying Song, Bing Song, Hailin Tian, Yen Wah Tong, To-Hung Tsui, David Wall, Haixia Wang, Endashaw Workie, Chao Xu, Lijie Xu, Xiaojun Yan, Miao Yan, Hairong Yuan, Le Zhang, Pengshuai Zhang, Jingxin Zhang, Tengyu Zhang, Jun Zhao, Mi Zhou, Chengxu Zhou, Kang Zhou, Jie Fu J. Zhou, and Jie Zhou

Published
2022

18. Nonwaste technology in the bioethanol and biodiesel industries

Author

Luciana Porto de Souza Vandenberghe, Nelson Libardi Junior, Kim Kley Valladares-Diestra, Gustavo Amaro Bittencourt, Ariane Fátima Murawski de Mello, Susan Grace Karp, Luiz Alberto Junior Letti, and Carlos Ricardo Soccol

Published
2022

19. Integrated sugarcane biorefinery for first- and second-generation bioethanol production using imidazole pretreatment

Author

Kim Kley Valladares-Diestra, Luciana Porto de Souza Vandenberghe, Verônica Sayuri Nishida, Luis Alberto Zevallos Torres, Arion Zandoná Filho, and Carlos Ricardo Soccol

Subjects
Renewable Energy, Sustainability and the Environment, Strategy and Management, Building and Construction, Industrial and Manufacturing Engineering, General Environmental Science
Published
2022

20. Added-value biomolecules' production from cocoa pod husks: A review

Author

Carlos Ricardo Soccol, Gustavo Amaro Bittencourt, Gilberto Vinícius de Melo Pereira, Luciana Porto de Souza Vandenberghe, Ariane Fátima Murawski de Mello, Kim Kley Valladares-Diestra, Priscilla Zwiercheczewski de Oliveira, and Zulma Sarmiento Vásquez

Subjects
Cacao, Environmental Engineering, Renewable Energy, Sustainability and the Environment, business.industry, Bioengineering, General Medicine, COCOA BEAN, Raw material, Husk, food.food, Biotechnology, food, Bioenergy, Agriculture, Food, Added value, Production (economics), Biomass, Valorisation, business, Waste Management and Disposal
Abstract

Cocoa beans are produced through on-farm processing where residual biomass is discarded, including cocoa pod husks (CPH), cocoa bean shells and cocoa sweatings. CPH represents about 80% of these residues that are generated during the initial cocoa bean processing steps and their disposal occupies large areas, causing social and environmental concerns. In the last decades, the lignocellulosic composition of CPH has attracted the attention of the scientific and productive sector. Recently, some studies have reported the use of CPH in the production of medium to high value-added molecules, with potential applications in food and feed, agriculture, bioenergy, and other segments. This review presents biotechnological approaches and processes for the exploitation of CPH, including pre-treatment methods for the production of different biomolecules. Great perspectives and innovations were found concerning CPH exploitation and valorisation, but still more efforts are needed to valorise this potential feedstock and give support to producers in-development countries.

Published
2021

22. Integrated xylooligosaccharides production from imidazole-treated sugarcane bagasse with application of in house produced enzymes

Author

Kim Kley, Valladares-Diestra, Luciana, Porto de Souza Vandenberghe, and Carlos Ricardo, Soccol

Subjects
Endo-1,4-beta Xylanases, Environmental Engineering, Renewable Energy, Sustainability and the Environment, Hydrolysis, Imidazoles, Oligosaccharides, Glucuronates, Bioengineering, General Medicine, Cellulose, Waste Management and Disposal, Saccharum
Abstract

The application of biorefinery concepts to produce different value-added biomolecules such as xylooligosaccharides (XOs) generates economical competitive, sustainable and environmentally friendly processes. The objective of this work was to develop an efficient imidazole-pretreatment process of sugarcane bagasse (SB) and the use of the obtained hemicellulose fraction in the production of XOs with the application of in house produced xylanolytic enzymes using SB as substrate, under a biorefinery approach. SB imidazole pretreatment allowed the recovery of a hemicellulose rich fraction (34%) with 91.2% of delignification. Xylanase production by Aspergillus niger reached 53.1 U·mL

Published
2022

23. Citric acid assisted hydrothermal pretreatment for the extraction of pectin and xylooligosaccharides production from cocoa pod husks

Author

Carlos Ricardo Soccol, Luis Alberto Zevallos Torres, Kim Kley Valladares-Diestra, Adenise Lorenci Woiciechowski, Arion Zandoná Filho, and Luciana Porto de Souza Vandenberghe

Subjects
Environmental Engineering, food.ingredient, Pectin, Renewable Energy, Sustainability and the Environment, Hydrolysis, Extraction (chemistry), Biomass, Oligosaccharides, Bioengineering, Glucuronates, General Medicine, Biorefinery, Husk, Citric Acid, chemistry.chemical_compound, food, chemistry, Cellulosic ethanol, Research Design, Enzymatic hydrolysis, Pectins, Food science, Citric acid, Waste Management and Disposal
Abstract

The main purpose of this work was the development of a new citric acid assisted hydrothermal pretreatment of cocoa pod husks (CPH), which has not yet been exploited for pectin recovery. CPHs pectin recovery was improved with concomitant production of xylooligosaccharides (XOS) through efficient enzymatic hydrolysis of the solid fraction. A central composite experimental design was planned to analyze the effect of pretreatment conditions. Under optimal conditions at 120 °C, 10 min and 2% w.v-1, the recovery of pectin accounted for 19.3% of the biomass submitted to pretreatment with 52.2% of methyl esterification degree. Additionally, 51.9 mg.g−1 of XOS were also produced. The enzymatic conversion efficiency of the cellulosic fraction was 58.9%, leading to a production of 92.4 kg of glucose per ton of CPH. Great perspectives were observed in the implementation of CPH hydrothermal pretreatment for the production of value-added biomolecules under a biorefinery concept.

Published
2021

24. Soybean hulls as carbohydrate feedstock for medium to high-value biomolecule production in biorefineries: A review

Author

Zulma Sarmiento Vásquez, Kim Kley Valladares-Diestra, Leonardo Wedderhoff Herrmann, Carlos Ricardo Soccol, Susan Grace Karp, Gustavo Amaro Bittencourt, Ariane Fátima Murawski de Mello, and Luciana Porto de Souza Vandenberghe

Subjects
Environmental Engineering, Renewable Energy, Sustainability and the Environment, business.industry, Carbohydrates, Bioengineering, General Medicine, Raw material, Biorefinery, Pulp and paper industry, Culture Media, Bioenergy, Agriculture, Biofuel, Biofuels, Fermentation, Environmental science, Production (economics), Soybean hulls, Soybeans, Valorisation, business, Waste Management and Disposal
Abstract

Soybean is one of the major world crops, with an annual production of 359 million tons. Each ton of processed soybean generates 50–80 kg of soybean hulls (SHs), representing 5–8% of the whole seed. Due to environmental concerns and great economic potential, the search of SHs re-use solutions are deeply discussed. The lignocellulosic composition of SHs has attracted the attention of the scientific and productive sector. Recently, some studies have reported the use of SHs in the production of medium to high value-added molecules, with potential applications in food and feed, agriculture, bioenergy, and other segments. This review presents biotechnological approaches and processes for the management and exploitation of SHs, including pre-treatment methods and fermentation techniques, for the production of different biomolecules. Great potentialities and innovations were found concerning SH exploration and valorisation of the soybean chain under a biorefinery and circular bioeconomy optic.

Published
2021

25. A biorefinery approach for enzymatic complex production for the synthesis of xylooligosaccharides from sugarcane bagasse

Author

Kim Kley Valladares-Diestra, Luciana Porto de Souza Vandenberghe, and Carlos Ricardo Soccol

Subjects
0106 biological sciences, Enzyme complex, Environmental Engineering, Soybean meal, Continuous stirred-tank reactor, Oligosaccharides, Bioengineering, Glucuronates, 010501 environmental sciences, Raw material, complex mixtures, 01 natural sciences, 010608 biotechnology, Cellulose, Waste Management and Disposal, 0105 earth and related environmental sciences, Endo-1,4-beta Xylanases, Renewable Energy, Sustainability and the Environment, Chemistry, Hydrolysis, General Medicine, Biorefinery, Pulp and paper industry, Saccharum, Fermentation, Xylanase, Bagasse, Bubble column reactor
Abstract

The use of low-cost feedstock for enzyme production is an environmental and economic solution. Sugarcane bagasse and soybean meal are employed in this study for optimised xylanase production with the concomitant synthesis of proteases. The enzymatic complex is produced by submerged fermentation by Aspergillus niger. Optimisation steps lead to a 2.16-fold increase in enzymatic activity. The fermentation kinetics are studied in Erlenmeyer flasks, a stirred tank reactor and a bubble column reactor, with the xylanase activities reaching 52.9; 33.7 and 60.5 U.mL−1, respectively. The protease production profile is also better in the bubble column reactor, exceeding 7 U.mL−1. The enzyme complex is then evaluated for the synthesis of xylooligosaccharides from sugarcane extracted xylan with a production of 3.1 g.L−1 where xylotriose is the main product. Excellent perspectives are observed for the developed process with potential applications in the animal feed, prebiotics and paper industries.

Published
2021

26. List of contributors

Author

Muhammad Faiq Abdullah, Gustavo Amaro Bittencourt, K.B. Arun, Parameswaran Binod, Rafaela de Oliveira Penha, Alvina Farooqui, Pedram Fatehi, Prachi Gaur, Vivek Kumar Gaur, Krishna Gautam, Gan Pei Gie, Mohd. Ishfaq Bhat, Maria Sindhura John, Susan Grace Karp, Rajwinder Kaur, Kim Kley Valladares-Diestra, Mohan Konduri, Fangong Kong, Niveditha Kulangara, Lalit R. Kumar, Virendra Kumar, Gabriel Sprotte Kumlehn, Aravind Madhavan, Antônio Irineudo Magalhães Junior, Sara Magdouli, Natesan Manickam, Mitali Mishra, Raj Morya, Ariane Fátima Murawski de Mello, Joseph Amruthraj Nagoth, Taru Negi, Mohd Firdaus Omar, Maria Giovana Binder Pagnoncelli, Aishwarya Pandey, Ashok Pandey, Ashutosh Kumar Pandey, Kritika Pandey, Srinath Pandey, Eapen Philip, Sameer Pokhrel, Luciana Porto de Souza Vandenberghe, Fernanda Prado, Nalla Bhanu Prakash Reddy, R. Reshmy, Thana Saffar, Preety Saini, Ayyoub Salaghi, Aditi Sharma, Poonam Sharma, Raveendran Sindhu, Lalit Kumar Singh, Shikhangi Singh, Ranjna Sirohi, Carlos Ricardo Soccol, Aditya Srivastava, Janmejai Kumar Srivastava, Swapna Thacheril Sukumaran, Ayon Tarafdar, Indu Shekhar Thakur, Sam Sung Ting, Bhagyashree Tiwari, Bhawna Tyagi, R.D. Tyagi, Sudha Upadhyay, P.H. Vaisakh, Narisetty Vivek, Fazli Wahid, Bhoomika Yadav, Sravan Kumar Yellapu, Kaiser Younis, Cheng Zhong, and Long Zhou

Published
2021

27. Xylan

Author

Luciana Porto de Souza Vandenberghe, Kim Kley Valladares-Diestra, Gustavo Amaro Bittencourt, Ariane Fátima Murawski de Mello, and Carlos Ricardo Soccol

Published
2021

28. Bioethanol and succinic acid co-production from imidazole-pretreated soybean hulls

Author

Adenise Lorenci Woiciechowski, Luciana Porto de Souza Vandenberghe, Arion Zandoná Filho, Luis Alberto Zevallos Torres, Verônica Sayury Nishida, Carlos Ricardo Soccol, and Kim Kley Valladares-Diestra

Subjects
biology, food and beverages, Lignocellulosic biomass, Xylose, biology.organism_classification, Hydrolysate, chemistry.chemical_compound, Hydrolysis, chemistry, Succinic acid, Fermentation, Food science, Cellulose, Pichia stipitis, Agronomy and Crop Science
Abstract

The soybean is an important crop in the world, where soybean hull (SH) comprehends 8–10 % of grain dry matter. SH is rich in carbohydrates, mainly in holocellulose fibers, showing to be a promising bio-based material to generate new bio-products. Imidazole is a promissing solvent for pretreatment of lignocellulosic biomass due its delignificant and depolymerizing power. Besides, this reagent presents low to moderate toxicity against many classes of living organisms. Thus, the thermochemical conversion of SH into fermentable sugars using imidazole was carried out at 120 °C for 1 h. The objective of this work was to use a more sustainable pretreatment reagent for the co-production of second-generation bioethanol and succinic acid from soybean hull. After pretreatment, the solid yield was 57.1 wt.%, consisting principally of cellulose. The cellulose-rich fraction was further hydrolyzed using commercial enzymes and achieving 89.9 wt.% and 95.6 wt.% of glucose and xylose yields, respectively. The hydrolysate was fermented using two yeasts: Saccharomyces cerevisiae and Pichia stipitis. Approximately, from 1 ton of SH, it was estimated that 124.0 kg of bioethanol and 36.5 kg of succinic acid could be obtained using S. cerevisiae, while P. stipitis could achieve productions of 116.2 kg of bioethanol and 20.4 kg of the organic acid. This study demonstrated the co-production of biomolecules of industrial interests, carrying out a more environmentally friendly pretreatment process.

Published
2021

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