Search

Your search keyword '"Kim, Seong-Kwan"' showing total 45 results
Start Over Author "Kim, Seong-Kwan"
45 results on '"Kim, Seong-Kwan"'

9. Quantification of bupivacaine hydrochloride and isoflupredone acetate residues in porcine muscle, beef, milk, egg, shrimp, flatfish, and eel using a simplified extraction method coupled with liquid chromatography–triple quadrupole tandem mass spectrometry

Author

Cho, Sang-Hyun, Park, Jin-A, Zheng, Weijia, Abd El-Aty, A.M., Kim, Seong-Kwan, Choi, Jeong-Min, Yi, Hee, Cho, Soo-Min, Afifi, Nehal A., Shim, Jae-Han, Chang, Byung-Joon, Kim, Jin-Suk, and Shin, Ho-Chul

Published
2017
Full Text
View/download PDF

14. Additional file 1 of Effects of smoking on the tissue regeneration-associated functions of human endometrial stem cells via a novel target gene SERPINB2

Author

Park, Se-Ra, Kim, Seong-Kwan, Kim, Soo-Rim, Yu, Wook-Joon, Lee, Seung-Jin, and Lee, Hwa-Yong

Abstract

Additional file 1: Fig. S1. Establishment of human endometrial stem cells from uterine endometrial tissues. Isolated human endometrial stem cells based were observed under a phase-contrast microscope to assess their overall morphological features (a). Isolated human endometrial stem cells were positive for various stemness-associated antigens (CD44, CD73, CD105, CD140b, CD146, and susD2) and negative for multiple hematopoietic stem cell lineage antigens (CD34 and CD45) (b). Differentiation into adipocyte and osteoblast was analyzed by oil red O staining and alizarin red staining, respectively. Relative quantifications of calcium mineral contents and lipid droplet formation within differentiated cells were performed by measuring absorbance at 500 and 570 nm, respectively (c). All experiments were performed in triplicate. Data are presented as mean ± SDs. *, p< 0.05; **, p< 0.005; and ***, p< 0.001 (two-sample t test). Fig. S2. Efficacy of SERPINB2 knockdown using specific shRNAs in endometrial stem cells. Human endometrial stem cells were transfected with multiple shRNAs (#1, #2, #3, #5, or #4), which specifically target SERPINB2, or with a non-targeting control shRNA (a). shRNA construct #2 (hereafter referred to as SERPINB2 shRNA) most effectively knocked downSERPINB2 at the mRNA (b) and protein levels (c). β-actin was used as the internal control, and PPIA was used as a housekeeping gene for real-time PCR. All experiments were performed in triplicate. Data are presented as means ± SDs. *, p< 0.05; **, p< 0.005; and ***, p< 0.001 (two-sample t test). Fig. S3. Efficiency of inducing SERPINB2 overexpression using a specific retroviral expression vector. Endometrial stem cells were specifically transfected with a retroviral expression vector for SERPINB2 (a). Successful SERPINB2 overexpression was confirmed at the mRNA (b)and protein (c)levels. β-actin was used as internal control, and PPIA as the housekeeping gene for real-time PCR. All experiments were performed in triplicate. Data are presented as mean ± SDs. *, p< 0.05; **, p< 0.005; and ***, p< 0.001 (two-sample t test). Fig. S4. The cigarette smoke-induced inhibitory effects on various growth factor secretions are closely interacted with self-renewal capacity-associated signaling pathways. Differentially activated signaling molecules secreted from toxic substance-exposed and non-exposed cells were analyzed using IPA software to predict their activation states (activated or inhibited) with respect to AR (GSE69851) (a) and EGFR(GSE60408) (b) associated signaling integrities. Fig. S5. The cigarette smoke-induced inhibitory effects on various growth factor secretions are closely interacted with self-renewal capacity-associated signaling pathways. Differentially activated signaling molecules in toxic substance-exposed cells and non-exposed cells were analyzed using IPA software to predict their activation states (activated or inhibited) with respect to CSF-1 (GSE69851) (a) or VEGFR3 (GSE60408) (b)associated signaling integrities. Fig. S6. Functional interactions between cigarette smoke-induced factors and signaling networks that regulate multiple essential cellular functions. Signaling network analysis was conducted using GeneMANIA (http://www.genemania.org) to analyze interactions between the cigarette smoke-induced changes of eight growth factors (AR, EGFR, IGF-1, M-CSF/CSF1, NT-4, PDGF-AA, TGFβ3, and VEGFR3) and signaling networks that regulate self-renewal, pluripotency/stemness, or migratory capacity. The results showed strong interaction between these eight prominent growth factors and various essential cellular functions, such as proliferative capacity, pluripotency/stemness, and migration potential (a, b). Fig. S7. Cigarette smoke exposure markedly suppressed various tissue regeneration-associated functions of adipose tissue-derived stem cells in vivo. Schematic of the in vivo experimental procedure described in Materials and Methods (a). Mice were intraperitoneally administrated a low (0.5 mg/kg) or high (1 mg/kg) dose of cigarette smoke extract or vehicle (medium) 10 times. Adipose tissue-derived stem cells were then isolated from mouse adipose tissues, and the inhibitory effects of cigarette smoke exposure on cell viability were assessed using an MTT assay (b). The changes in stem cell migratory abilities were determined using a transwell assay (c)and by western blotting using MMP-2 and MMP-9 antibodies (d). The inhibitory effects of cigarette smoke extract on the differentiation of mouse adipose tissue-derived stem cells into adipocytes (e) and osteoblast (f) in vivo were analyzed by oil red O and alizarin red staining, respectively. Relative quantifications of calcium mineral contents and lipid droplet formation within differentiated cells were evaluated by measuring absorbance at 500 and 570 nm, respectively. The inhibitory effects of cigarette smoke exposure on the expressions of various pluripotency-associated genes, C-MYC, KLF4, NANOG, OCT4, and SOX2, were determined by real-time PCR (g). β-actin was used as the internal control, and PPIA as the housekeeping gene for real-time PCR analysis. All experiments were performed in triplicate. Data are presented as means ± SDs. *, p< 0.05; **, p< 0.005; and ***, p< 0.001 (by the two-sample t test). Fig. S8. Cigarette smoke exposure markedly suppressed various tissue regeneration-associated functions of bone marrow-derived stem cells in vivo. Schematic of the in vivo experimental procedure described in Materials and Methods (a). Mice were intraperitoneally administrated low (0.5 mg/kg) or high (1 mg/kg) dose of cigarette smoke extract 10 times or vehicle (medium). Bone marrow-derived stem cells were isolated from bone marrow, and the inhibitory effects of cigarette smoke exposure on cell viability were assessed using an MTT assay (b). The changes in stem cell migratory abilities were analyzed using a transwell assay (c)and western blotting was performed using MMP-2 and MMP-9 antibodies (d). The inhibitory effects of cigarette smoke exposure on the differentiations of mouse bone marrow-derived stem cells into adipocytes (e) or osteoblasts (f) in vivo were assessed by oil red O and alizarin red staining, respectively. Relative quantifications of calcium mineral contents and lipid droplet formation within differentiated cells were performed by measuring absorbance at 500 and 570 nm, respectively. The inhibitory effects of cigarette smoke exposure on the expressions of various pluripotency-associated genes, C-MYC, KLF4, NANOG, OCT4, and SOX2, were analyzed by real-time PCR (g). β-actin was used as the internal control, and PPIA as the housekeeping gene for real-time PCR analysis. All experiments were performed in triplicate. Data are presented as means ± SDs. *, p< 0.05; **, p< 0.005; and ***, p< 0.001 (two-sample t test).

Published
2022
Full Text
View/download PDF

15. Residue analysis of tebufenozide and indoxacarb in chicken muscle, milk, egg and aquatic animal products using liquid chromatography–tandem mass spectrometry

Author

Choi, Jeong‐Min, primary, Zheng, Weijia, additional, Abd El‐Aty, A. M., additional, Kim, Seong‐Kwan, additional, Park, Da‐Hee, additional, Yoo, Kyung‐Hee, additional, Lee, Gyu‐Hee, additional, Baranenko, Denis A., additional, Hacımüftüoğlu, Ahmet, additional, Jeong, Ji Hoon, additional, Kang, Young‐Sun, additional, and Shin, Ho‐Chul, additional

Published
2019
Full Text
View/download PDF

16. Quantitative determination of carbasalate calcium derived metabolites, acetylsalicylic acid and salicylic acid, in six animal foods using liquid-liquid extraction method coupled with liquid chromatography-tandem mass spectrometry

Author

Zheng, Weijia, primary, Yoo, Kyung-Hee, additional, Abd El-Aty, A.M., additional, Park, Da-Hee, additional, Choi, Jeong-Min, additional, Kim, Seong-Kwan, additional, Kang, Young-Sun, additional, Zhang, Hongxia, additional, Hacımüftüoğlu, Ahmet, additional, Bekhit, Alaa El-Din, additional, Wang, Jing, additional, Shim, Jae-Han, additional, and Shin, Ho-Chul, additional

Published
2019
Full Text
View/download PDF

17. Development and validation of a solid‐phase extraction method coupled with LC–MS/MS for the simultaneous determination of 16 antibiotic residues in duck meat

Author

Zheng, Weijia, primary, Abd El‐Aty, A. M., additional, Kim, Seong‐Kwan, additional, Choi, Jeong‐Min, additional, Park, Da‐Hee, additional, Yoo, Kyung‐Hee, additional, Kang, Young‐Sun, additional, Jeon, Jong‐Sup, additional, Hacımüftüoğlu, Ahmet, additional, Shim, Jae‐Han, additional, and Shin, Ho‐Chul, additional

Published
2019
Full Text
View/download PDF

18. Simultaneous determination of spinosad, temephos, and piperonyl butoxide in animal‐derived foods using LC–MS/MS

Author

Zheng, Weijia, primary, Choi, Jeong‐Min, additional, Abd El‐Aty, A. M., additional, Yoo, Kyung‐Hee, additional, Park, Da‐Hee, additional, Kim, Seong‐Kwan, additional, Kang, Young‐Sun, additional, Hacımüftüoğlu, Ahmet, additional, Wang, Jing, additional, Shim, Jae‐Han, additional, and Shin, Ho‐Chul, additional

Published
2019
Full Text
View/download PDF

19. Residual detection of naproxen, methyltestosterone and 17α-hydroxyprogesterone caproate in aquatic products by simple liquid-liquid extraction method coupled with liquid chromatography-tandem mass spectrometry

Author

Zheng, Weijia, primary, Yoo, Kyung-Hee, additional, Choi, Jeong-Min, additional, Park, Da-Hee, additional, Kim, Seong-Kwan, additional, Kang, Young-Sun, additional, Abd El-Aty, A. M., additional, Hacımüftüoğlu, Ahmet, additional, Wang, Jing, additional, Shim, Jae-Han, additional, and Shin, Ho-Chul, additional

Published
2018
Full Text
View/download PDF

22. Corrigendum to “Simultaneous determination of clanobutin, dichlorvos, and naftazone in pork, beef, chicken, milk, and egg using liquid chromatography–tandem mass spectrometry” [Food Chem. 252C (2018) 40–48]

Author

Park, Jin-A., primary, Abd El-Aty, A.M., additional, Zheng, Weijia, additional, Kim, Seong-Kwan, additional, Cho, Sang-Hyun, additional, Choi, Jeong-min, additional, Hacımüftüoğlu, Ahmet, additional, Jeong, Ji Hoon, additional, Wang, Jing, additional, Shim, Jae-Han, additional, and Shin, Ho-Chul, additional

Published
2018
Full Text
View/download PDF

23. Simultaneous determination of clanobutin, dichlorvos, and naftazone in pork, beef, chicken, milk, and egg using liquid chromatography-tandem mass spectrometry

Author

Park, Jin-A., primary, Abd El-Aty, A.M., additional, Zheng, Weijia, additional, Kim, Seong-Kwan, additional, Cho, Sang-Hyun, additional, Choi, Jeong-min, additional, Hacımüftüo, Ahmet, additional, Jeong, Ji Hoon, additional, Wang, Jing, additional, Shim, Jae-Han, additional, and Shin, Ho-Chul, additional

Published
2018
Full Text
View/download PDF

25. Development and validation of a simple solid-phase extraction method coupled with liquid chromatography-triple quadrupole tandem mass spectrometry for simultaneous determination of lincomycin, tylosin A and tylosin B in royal jelly

Author

Zheng, Weijia, primary, Park, Jin-A, additional, Abd El-Aty, A. M., additional, Kim, Seong-Kwan, additional, Cho, Sang-Hyun, additional, Choi, Jeong-Min, additional, Warda, Mohamad, additional, Wang, Jing, additional, Shim, Jae-Han, additional, and Shin, Ho-Chul, additional

Published
2017
Full Text
View/download PDF

26. Residual detection of buparvaquone, nystatin, and etomidate in animal-derived food products in a single chromatographic run using liquid chromatography-tandem mass spectrometry

Author

Zhang, Dan, primary, Park, Jin-A., additional, Abd El-Aty, A.M., additional, Kim, Seong-Kwan, additional, Cho, Sang-Hyun, additional, Wang, Yanmei, additional, Shim, Jae-Han, additional, Jeong, Ji Hoon, additional, Shin, Sung Chul, additional, Kim, Jin-Suk, additional, Chang, Byung-Joon, additional, and Shin, Ho-Chul, additional

Published
2017
Full Text
View/download PDF

27. Determination of fenobucarb residues in animal and aquatic food products using liquid chromatography-tandem mass spectrometry coupled with a QuEChERS extraction method

Author

Zheng, Weijia, primary, Park, Jin-A, additional, Zhang, Dan, additional, Abd El-Aty, A.M., additional, Kim, Seong-Kwan, additional, Cho, Sang-Hyun, additional, Choi, Jeong-Min, additional, Shim, Jae-Han, additional, Chang, Byung-Joon, additional, Kim, Jin-Suk, additional, and Shin, Ho-Chul, additional

Published
2017
Full Text
View/download PDF

28. Sustainability of CD24 expression, cell proliferation and migration, cisplatin-resistance, and caspase-3 expression during mesenchymal-epithelial transition induced by the removal of TGF-β1 in A549 lung cancer cells

Author

Kim, Seong-Kwan, primary, Park, Jin-A, additional, Zhang, Dan, additional, Cho, Sang-Hyun, additional, Yi, Hee, additional, Cho, Soo-Min, additional, Chang, Byung-Joon, additional, Kim, Jin-Suk, additional, Shim, Jae-Han, additional, Abd El-Aty, A. M., additional, and Shin, Ho-Chul, additional

Published
2017
Full Text
View/download PDF

30. Residual detection of naproxen, methyltestosterone and 17α‐hydroxyprogesterone caproate in aquatic products by simple liquid–liquid extraction method coupled with liquid chromatography–tandem mass spectrometry.

Author

Zheng, Weijia, Yoo, Kyung‐Hee, Choi, Jeong‐Min, Park, Da‐Hee, Kim, Seong‐Kwan, Kang, Young‐Sun, Abd El‐Aty, A. M., Hacımüftüoğlu, Ahmet, Wang, Jing, Shim, Jae‐Han, and Shin, Ho‐Chul

Abstract

In the present study, we aimed to develop a reliable screening method based on liquid chromatography coupled with tandem mass spectrometry (LC–MS/MS) for the detection and quantification of naproxen, methyltestosterone and 17α‐hydroxyprogesterone caproate residues. The target analytes were extracted from samples of eel, flatfish and shrimp using acetonitrile with 1% acetic acid, followed by liquid–liquid purification with n‐hexane. Chromatographic separation was achieved on a reversed‐phase analytical column using 0.1% formic acid containing 10 mm ammonium formate in distilled water (A) and methanol (B) as mobile phases. All the matrix‐matched calibration curves were linear (R2 ≥ 0.99) over the concentration range of the tested analytes. Recovery at three spiking levels (0.005, 0.01 and 0.02 mg/kg) ranged from 68 to 117% with intra‐ and inter‐day precisions <10%. Five market samples for each matrix (eel, flatfish and shrimp) were collected and tested for method application. In summary, the proposed method is feasible to screen and quantify the analytes with high selectivity in aquatic food products meant for human consumption. [ABSTRACT FROM AUTHOR]

Published
2019
Full Text
View/download PDF

33. Development of a high-performance liquid chromatography with fluorescence detection method for quantification of piperazine in animal products by using precolumn derivatization

Author

Park, Jin-A, primary, Zhang, Dan, additional, Kim, Dong-Soon, additional, Kim, Seong-Kwan, additional, Cho, Sang-Hyun, additional, Jeong, Daun, additional, Kim, Jin-Suk, additional, Shim, Jae-Han, additional, Abd El-Aty, A.M., additional, and Shin, Ho-Chul, additional

Published
2016
Full Text
View/download PDF

34. A simple extraction method for the simultaneous detection of tetramisole and diethylcarbamazine in milk, eggs, and porcine muscle using gradient liquid chromatography–tandem mass spectrometry

Author

Zhang, Dan, primary, Park, Jin-A, additional, Kim, Dong-Soon, additional, Kim, Seong-Kwan, additional, Shin, Soo-Jean, additional, Shim, Jae-Han, additional, Shin, Sung Chul, additional, Kim, Jin-Suk, additional, Abd El-Aty, A.M., additional, and Shin, Ho-Chul, additional

Published
2016
Full Text
View/download PDF

35. Determination of residual levels of naloxone, yohimbine, thiophanate, and altrenogest in porcine muscle using QuEChERS with liquid chromatography and triple quadrupole mass spectrometry

Author

Zhang, Dan, primary, Park, Jin‐A, additional, Kim, Seong‐Kwan, additional, Cho, Sang‐Hyun, additional, Cho, Soo‐Min, additional, Yi, Hee, additional, Shim, Jae‐Han, additional, Kim, Jin‐Suk, additional, Abd El‐Aty, A. M., additional, and Shin, Ho‐Chul, additional

Published
2016
Full Text
View/download PDF

36. Development and validation of a simple solid‐phase extraction method coupled with liquid chromatography–triple quadrupole tandem mass spectrometry for simultaneous determination of lincomycin, tylosin A and tylosin B in royal jelly.

Author

Zheng, Weijia, Park, Jin‐A, Abd El‐Aty, A. M., Kim, Seong‐Kwan, Cho, Sang‐Hyun, Choi, Jeong‐Min, Warda, Mohamad, Wang, Jing, Shim, Jae‐Han, and Shin, Ho‐Chul

Abstract

Abstract: We have developed an analytical method for the determination of lincomycin, tylosin A and tylosin B residues in royal jelly using liquid chromatography–triple quadrupole tandem mass spectrometry analysis. For extraction and purification, we employed 1% trifluoroacetic acid and 0.1  m Na2EDTA solutions along with an Oasis HLB cartridge. The target antibiotics were well separated in a Kinetex EVO C18 reversed‐phase analytical column using a combination of 0.1% formate acid in ultrapure water (A) and acetonitrile (B) as the mobile phase. Good linearity was achieved over the tested concentration range (5–50 μg/kg) in matrix‐matched standard calibration. The coefficients of determination (R2) were 0.9933, 0.9933 and 0.996, for tylosin A, tylosin B and lincomycin, respectively. Fortified royal jelly spiked with three different concentrations of the tested antibiotics (5, 10 and 20 μg/kg) yielded recoveries in the range 80.94–109.26% with relative standard deviations ≤4%. The proposed method was applied to monitor 11 brand of royal jelly collected from domestic markets and an imported brand from New Zealand; all the samples tested negative for lincomycin, tylosin A and tylosin B residues. In conclusion, 1% trifluoroacetic acid and 0.1  m Na2EDTA aqueous solvents combined with solid‐phase extraction could effectively complete the sample preparation process for royal jelly before analysis. The developed approach can be applied for a routine analysis of lincomycin, tylosin A and tylosin B residues in royal jelly. [ABSTRACT FROM AUTHOR]

Published
2018
Full Text
View/download PDF

42. Simple extraction method for quantification of phenothiazine residues in pork muscle using liquid chromatography-triple quadrupole tandem mass spectrometry.

Author

Zhang, Dan, Park, Jin‐A, Kim, Seong‐Kwan, Cho, Sang‐Hyun, Cho, Soo‐Min, Shim, Jae‐Han, Kim, Jin‐Suk, Abd El‐Aty, A. M., and Shin, Ho‐Chul

Abstract

In this study, an analytical method was developed for quantification of residues of the anthelmintic drug phenothiazine (PTZ) in pork muscle using liquid chromatography-tandem mass spectrometry. Muscles were extracted using 0.2% formic acid and 10 m m ammonium formate in acetonitrile, defatted and purified using n-hexane. The drug was well separated on a Waters XBridge™ C18 analytical column using a binary solvent system consisting of 0.2% formic acid and 10 m m ammonium formate in ultrapure water (A) and acetonitrile (B). Good linearity was achieved over a six-point concentration range in matrix-matched calibration with determination coefficient =0.9846. Fortified pork muscle having concentrations equivalent to and double the limit of quantification (1 ng/g) yielded recovery ranges between 100.82 and 104.03% and relative standard deviations <12%. Samples ( n = 5) collected from large markets located in Seoul City tested negative for PTZ residue. In conclusion, 0.2% formic acid and ammonium formate in acetonitrile can effectively extract PTZ from pork muscle without solid-phase extraction, a step normally required for cleanup before analysis and the validated method can be used for routine analysis to ensure the quality of animal products. [ABSTRACT FROM AUTHOR]

Published
2017
Full Text
View/download PDF

44. A modified QuEChERS method coupled with liquid chromatography-tandem mass spectrometry for the simultaneous detection and quantification of scopolamine, L-hyoscyamine, and sparteine residues in animal-derived food products.

Author

Zheng W, Yoo KH, Choi JM, Park DH, Kim SK, Kang YS, Abd El-Aty AM, Hacımüftüoğlu A, Jeong JH, Bekhit AE, Shim JH, and Shin HC

Abstract

We developed a modified Quick, Easy, Cheap, Effective, Rugged, and Safe (CEN QuEChERS) extraction method coupled with liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI + /MS-MS) to identify and quantify residues of three botanical alkaloids, namely, scopolamine, L-hyoscyamine, and sparteine, in animal-derived foods, including porcine muscle, egg, and milk. A combination of ethylenediaminetetraacetic acid disodium buffer and acetonitrile acidified with 0.5% trifluoroacetic acid was used as an extraction solvent, whereas QuEChERS (CEN, 15662) kits and sorbents were applied for cleanup procedures. The proposed method was validated by determining the limits of quantification (LOQs), with values of 1-5 µg/kg achieved for the target analytes in various matrices. Linearity was estimated from matrix-matched calibration curves constructed using six concentration levels ranging from 1- to 6-fold increases in the LOQs of each analyte, and the correlation coefficients ( R 2 ) were ≥0.9869. Recoveries (at three concentration levels of 1-, 2-, and 3-fold increases in the LOQ) of 73-104% were achieved with relative standard deviations (RSDs) ≤7.7% (intra-day and inter-day precision). Ten types of each matrix procured from large markets were evaluated, and all tested samples showed negative results. The current protocol is simple and versatile and can be used for routine detection of plant alkaloids in animal food products.

Published
2018
Full Text
View/download PDF

45. Determination of imidocarb in animal biosamples using simple high-performance liquid chromatography with diode-array detection.

Author

Park JA, Cho HJ, Cho SM, Kim NH, Kim DS, Zhang D, Kim SK, Yi H, and Shin HC

Abstract

The aim of this study was to develop an analytical method for detection of imidocarb [1,3-bis[3-(4,5-dihydro-1h-imidazol-2-yl)phenyl]urea] in beef and milk using high-performance liquid chromatography (HPLC) with diode-array detection (DAD). Imidocarb was separated on a reversed-phase column (4.6×250 mm, 5 μm) with a mobile phase consisting of 85:15 (v/v) 0.1% trifluoroacetic acid/acetonitrile. The flow rate was 1 ml/min, and the column temperature was maintained at 20°C. Detection was carried out at 260 nm using a DAD detector. The analytical samples were extracted using a solid-phase extraction (SPE) method. The calibration curves showed good linearity (r≥0.998). Limits of quantifications (LOQs) were 0.15 mg/kg in beef and 0.025 mg/kg in milk. Intra- and inter-day precisions were 3.2-6.1 and 1.4-6.9%, respectively, and the accuracy (recovery) was 80.4-82.2% and 80.1-89.5% in beef and milk, respectively. Thus, an analytical protocol using SPE extraction followed by HPLC with DAD was successfully developed, which demonstrated acceptable precision and recovery.

Published
2014
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results