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Hypertension is a major risk factor for both stroke and cognitive impairment, but it is unclear whether it may specifically affect post-stroke cognitive impairment. We assessed the effect of hypertension and/or stroke on brain injury, cognitive outcome, and the brain transcriptomic profile. C57BL/6J mice (n = 117; 3–5 mo.) received s.c. infusion of either saline or angiotensin II followed by sham surgery or photothrombotic stroke targeting the prefrontal cortex seven days later. Cognitive function was assessed with the Barnes maze and RNA sequencing was used to quantify transcriptomic changes in the brain. Angiotensin II treatment produced spontaneous hemorrhaging after stroke. In the Barnes maze, hypertensive mice that received stroke surgery had an increased escape latency compared to other groups (day 3: hypertensive + stroke = 166.6 ± 6.0 s vs. hypertensive + sham = 122.8 ± 13.8 s vs. normotensive + stroke = 139.9 ± 10.1 s vs. normotensive + sham = 101.9 ± 16.7 s), consistent with impaired cognition. RNA sequencing revealed >1500 differentially expressed genes related to neuroinflammation in hypertensive + stroke vs. normotensive + stroke, which included genes associated with apoptosis, microRNAs, autophagy, anti-cognitive biomarkers and Wnt signaling. Overall, we show that the combination of hypertension and stroke resulted in greater learning impairment and brain injury. [ABSTRACT FROM AUTHOR]

Objective To document the experiences of converting a general hospital to a coronavirus disease 2019 (COVID-19) designated hospital during an outbreak in Daegu, Republic of Korea. Methods The hospital management formed an emergency task force team, whose role was to organize the COVID-19 hospital. The task force used different collaborative channels to redistribute resources and expertise to the hospital. Leading doctors from the departments of infectious diseases, critical care and pulmonology developed standardized guidelines for treatment coherence. Nurses from the infection control team provided regular training on donning and doffing of personal protective equipment and basic safety measures. Findings Keimyung University Daegu Dongsan hospital became a red zone hospital for COVID-19 patients on 21 February 2020. As of 29 June 2020, 1048 COVID-19 patients had been admitted to the hospital, of which 22 patients died and five patients were still being treated in the recovery ward. A total of 906 health-care personnel worked in the designated hospital, of whom 402 were regular hospital staff and 504 were dispatched health-care workers. Of these health-care workers, only one dispatched nurse acquired COVID-19. On June 15, the hospital management and Daegu city government decided to reconvert the main building to a general hospital for non-COVID-19 patients, while keeping the additional negative pressure rooms available, in case of resurgence of the disease. Conclusion Centralized coordination in frontline hospital operation, staff management, and patient treatment and placement allowed for successful pooling and utilization of medical resources and manpower during the COVID-19 outbreak. Objectif Documenter la transformation d'un hopital general en hopital de reference pour la maladie a coronavirus 2019 (COVID-19) durant une epidemie a Daegu, en Republique de Coree. Methodes La direction de l'hopital a constitue une equipe operationnelle d'urgence chargee de l'organisation au sein de l'hopital COVID-19. Cette equipe a employe differents moyens de collaboration pour rediriger les ressources et l'expertise vers l'hopital. Les medecins a la tete des departements des maladies infectieuses, des soins intensifs et de pneumologie ont formule des directives standardisees afin d'assurer un traitement coherent. Les infirmieres affectees au controle des infections ont regulierement dispense des formations consacrees au port et au retrait des equipements de protection personnelle, ainsi qu'aux mesures de securite elementaires. Resultats Le 21 fevrier 2020, l'hopital Keimyung University Daegu Dongsan est devenu un etablissement de zone rouge pour les patients atteints de la COVID-19. Entre cette date et le 29 juin 2020, il a admis 1048 patients COVID-19 et deplore 22 deces; cinq patients sont toujours hospitalises en soins intensifs. Au total, 906 professionnels de la sante ont travaille dans cet hopital de reference: 402 faisaient deja partie du personnel de l'etablissement et 504 y ont ete depeches. Sur l'ensemble de ces soignants, seule une infirmiere envoyee sur place a contracte la COVID-19. Le 15 juin, la direction de l'hopital et les autorites municipales ont decide de reconvertir le batiment principal en hopital general afin d'accueillir les patients non atteints par la COVID-19, tout en gardant les chambres a pression negative supplementaires pour faire face a une eventuelle recrudescence de l'epidemie. Conclusion La coordination centralisee du fonctionnement de l'hopital en premiere ligne, la gestion du personnel ainsi que le traitement et le placement des patients ont permis de regrouper et d'exploiter au mieux les ressources medicales et humaines durant l'epidemie de COVID-19. Objetivo Registrar las experiencias de la conversion de un hospital general en un hospital designado para la enfermedad por coronavirus de 2019 (COVID-19) durante un brote epidemico en Daegu, Republica de Corea. Metodos La direccion del hospital constituyo un equipo de trabajo de emergencia, cuya funcion era organizar el hospital COVID-19. El equipo de tareas utilizo diferentes medios de colaboracion para redistribuir los recursos y los conocimientos tecnicos al hospital. Los medicos mas destacados de los servicios de enfermedades infecciosas, cuidados intensivos y neumologia elaboraron unas directrices normalizadas para asegurar la uniformidad de los tratamientos. Los enfermeros del equipo de control de infecciones imparten capacitacion de manera regular sobre como ponerse y quitarse el equipo de proteccion personal y sobre las medidas de seguridad basicas. Resultados El hospital Keimyung University Daegu Dongsan se convirtio en un hospital de la zona roja para los pacientes con la COVID-19 el 21 de febrero de 2020. Al 29 de junio de 2020, 1048 pacientes con la COVID-19 fueron admitidos en el hospital, de los que 22 murieron y 5 fueron tratados en la planta de recuperacion. 906 profesionales sanitarios trabajaban en el hospital designado, de los que 402 formaban parte del personal ordinario del hospital y 504 eran profesionales sanitarios temporales. De estos profesionales sanitarios, solo 1 enfermero temporal adquirio la COVID-19. El 15 de junio, la direccion del hospital y el gobierno municipal de Daegu decidieron reconvertir el edificio principal en un hospital general para los pacientes que no tenian la COVID-19, conservando las salas adicionales de presion negativa que se habian construido, en caso de que la enfermedad volviera a presentarse. Conclusion La coordinacion centralizada en el funcionamiento de la primera linea del hospital, la gestion del personal y el tratamiento y la ubicacion de los pacientes permiten la agrupacion y el uso satisfactorio de los recursos medicos y de la fuerza de trabajo durante el brote epidemico de la COVID-19. [phrase omitted], Introduction The coronavirus disease 2019 (COVID-19) outbreak has affected almost all countries in the world. Such health crises call for hospitals and other health-care facilities to develop strategies to manage [...]