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The excited-state tautomer equilibrium of the urea-fused single-benzene fluorophore was synthetically modulated to produce exceptionally large Stokes shifts (>12 400 cm−1). The key N–H⋯N hydrogen bonding motif utilizes an endogenous proton for long-wavelength emission or an exogenous proton for acid–base chemistry, the balance of which is exploited for fluorescence switching in the solid state. [ABSTRACT FROM AUTHOR]

Intermolecular interactions guide self-assembly on the surface. Precise control over these interactions by rational design of the molecule should allow fine control over the self-assembly patterns. Functional groups installed for electronic modulation often induce significant changes in the molecular dimensions, thereby disrupting the original assembly pattern. To overcome this challenge, we have employed a family of isosteric phenazine derivatives, DHP, DAP, and DBQD, to investigate the impacts of hydrogen bonding on two-dimensional molecular self-assembly. While these molecules are similar in size and chemical composition, the strength and directionality of hydrogen bonding differ significantly depending on the chemical structure of donor-acceptor pairs and prototropic tautomerization from positional isomerism. Scanning tunneling microscopy (STM) characterization of the assembled structures on Ag(111), Au(111), and Cu(100) surfaces revealed that minimal changes in molecular structure have a profound impact on the self-assembly patterns. While DHP exhibits highly ordered and robust assemblies, DAP and DBQD show either spatially confined or ill-defined assemblies. In conjunction with hydrogen bonding, prototropic tautomerism is a potent strategy to modulate molecular 2D lattices on surfaces., (© 2021 Wiley-VCH GmbH.)

Objectives: We developed and implemented a system for u-health (ubiquitous healthcare) services for patients with chronic obstructive pulmonary disease (COPD) and studied the level of acceptability by the patients in the context of home visits and telephone education., Subjects and Methods: To determine its effectiveness, the u-health service system was used for 2 years with 144 COPD patients in a clinical environment. Patients were provided with the u-healthcare service., Results: Patients with COPD were highly satisfied with the u-healthcare service, which incorporated telemonitoring, teleconsultation, and tele-education along with home visits. The average acceptable fees (in USD) of the service system are as follows: u-health device, $421.28; home visit, $21.53/visit; tele-education, $0.53/min or $26.57/month; and total service fee, $44.26/month., Conclusions: COPD patients accepted u-health services with a high satisfaction rate, promising a new paradigm of consumer-centric healthcare services.

Objectives: The study develops strategies for implementing ubiquitous healthcare (u-health) based on previous experiences of the healthcare professionals., Materials and Methods: Qualitative content analysis, one of qualitative research methods, was used on in-depth interviews conducted between July 21 and October 4, 2009, with 11 healthcare professionals including medical doctors and community healthcare specialist nurses who have previously provided u-health services., Results: Four primary subjects were addressed: (1) subjective experiences on the usability, (2) the expectations, (3) the business prospects, and (4) the prerequisites for the success of u-health market., Conclusion: Based on the results of this study, desirable u-health services from the perspective of healthcare professionals were proposed.

Vascular endothelial growth factors (VEGFs) are a family of proteins that mediate angiogenesis. VEGF165 is a VEGF-A isoform and has been extensively studied owing to its potential use in therapeutic angiogenesis. This study established Chinese hamster ovary (CHO) cells overexpressing recombinant human VEGF165 (rhVEGF165) protein. The production rate of the established CHO cells was over 80 mg/l of rhVEGF165 protein from a 7-day batch culture process using a 7.5-l bioreactor with a 5-l working volume and serum-free medium. The rhVEGF165 protein was purified to homogeneity from the culture supernatant using a two-step chromatographic procedure that resulted in a 48% recovery rate. The purified rhVEGF165 protein was a glycosylated homodimeric protein with a higher molecular weight (MW) than the protein expressed from insect cells, suggesting that the glycosylation of the rhVEGF165 protein in CHO cells differed from that in insect cells. The purified rhVEGF165 protein in this study was functionally active with a half-maximal effective concentration of 3.8 ng/ ml and specific activity of 2.5 x 105 U/mg.