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Although brushless excitation systems in synchronous machines have reduced the maintenance requirements and brush-related failures, they have increased the operating stresses on the excitation system components. Since the rotating excitation system is not accessible, it is difficult to detect excitation system failures such as open circuit diodes or open armature windings to prevent degradation in machine performance and reliability. Detection of such failures currently relies on monitoring the magnitude and harmonic content of the exciter field current. However, it is desirable to improve the reliability of fault detection with multiple test methods, considering the high cost of brushless exciter testing and repair due to the requirement of machine outage/disassembly. In this work, alternative off-line testing and on-line monitoring options are proposed for detection of excitation system failures. An off-line test based on ac excitation of the exciter field and on-line monitoring based on stator current and airgap flux analysis, are investigated, and fault indicators sensitive to excitation system failures are proposed for reliable fault monitoring. An experimental study on 3.8, 5.5, and 30 kW synchronous machines is given to validate the claims on the sensitivity and reliability of the proposed test methods. [ABSTRACT FROM AUTHOR]

Myoung, S.H.; Ji, H.-S.; Yu, H.-J.; Kim, H.J.; Kim, H.J., and Lee, B.-R., 2023. Ichthyoplankton assemblage structure in Jinhae Bay, Korea. In: Lee, J.L.; Lee, H.; Min, B.I.; Chang, J.-I.; Cho, G.T.; Yoon, J.-S., and Lee, J. (eds.), Multidisciplinary Approaches to Coastal and Marine Management. Journal of Coastal Research, Special Issue No. 116, 583-587. Charlotte (North Carolina), ISSN 0749-0208. Many studies on the species composition of ichthyoplankton have been conducted in Jinhae Bay. However, none have focused on the species' composition and distribution patterns. Therefore, herein, the species composition and distribution patterns of ichthyoplankton collected from January to December 2021 from Jinhae Bay, South Sea of Korea, were investigated. An oblique tow was collected from the bottom to the surface using a bongo net (80 cm, 330 µm) from eight stations. The collected samples were identified using morphological analysis and mtDNA cytochrome oxidase subunit I and 16S rRNA sequencing. A total of 66,938 ± 189,267 eggs/1000 m3 (mean ± Standard Deviation, SD) belonging to 30 species and 23 families, and 14,834 ± 31,325 larvae/1000 m3 from 45 species and 25 families, were collected from Jinhae Bay. The dominant egg species were Engraulis japonicus (93.4%), Repomucenus valenciennei (1.5%), and Nuchequula nuchalis (1.2%). Clupea pallasii (92.4%), R. valenciennei (2.5%), and E. japonicus (1.6%) were the dominant larval species. As a result of non-metric multidimensional scaling, fish were divided into two groups: One group spawns during low water temperatures and the other group spawns during high water temperatures. [ABSTRACT FROM AUTHOR]

Proteomic approaches using two-dimensional gel electrophoresis (2-DE) were adopted to identify proteins from rice leaf that are differentially expressed in response to the rice blast fungus, Magnaporthe grisea. Microscopic observation of inoculated leaf with M. grisea revealed that callose deposition and hypersensitive response was clearly visible in incompatible interactions but excessive invading hypha with branches were evident in compatible interactions. Proteins were extracted from leaves 24, 48, and 72 hours after rice blast fungus inoculation. Eight proteins resolved on the 2-DE gels were induced or increased in the inoculated leaf. Matrix-assisted laser desorption/ionization-time of flight analysis of these differentially displayed proteins showed them to be two receptor-like protein kinases (RLK), two beta-1.3-glucanases (Glu1, Glu2), thaumatin-like protein (TLP), peroxidase (POX 22.3), probenazole-inducible protein (PBZ1), and rice pathogenesis-related 10 (OsPR-10). Of these proteins, RLK, TLP, PBZ, and OsPR-10 proteins were induced more in the incompatible interactions than in compatible ones. A phytohormone, jasmonic acid also induced all eight proteins in leaves. To confirm whether the expression profile is equal to the 2-DE data, seven cDNA clones were used as probes in Northern hybridization experiments using total RNA from leaf tissues inoculated with incompatible and compatible rice blast fungal races. The genes encoding POX22.3, Glu1, Glu2, TLP, OsRLK, PBZ1, and OsPR-10 were activated in inoculated leaves, with TLP, OsRLK, PBZ1, and OsPR-10 being expressed earlier and more in incompatible than in compatible interactions. These results suggest that early and high induction of these genes may provide host plants with leading edges to defend themselves. The localization of two rice PR-10 proteins, PBZ1 and OsPR-10, was further examined by immunohistochemical analysis. PBZ1 accumulated highly in mesophyll cells under the attachment site of the appressorium. In contrast, OsPR-10 expression was mainly localized to vascular tissue.

To isolate high molecular weight (HMW) or low-abundance proteins we exploited the high resolving power provided by the molecular sieves of polyacrylamide gel matrices. Rice-leaf protein extracts were applied to a single well of an SDS-polyacrylamide gel with prestained molecular size markers at both ends. After electrophoresis, the gel was cut into 4 segments according to size, and each segment was ground in extraction buffer. The eluted proteins were separated from the gel matrix by centrifugation followed by acetone precipitation, and the precipitated proteins were subjected to SDS-PAGE and 2-DE. The SDS-PAGE-based prefractionation method provided non-overlapping discrete sample pools. About 27% more protein spots were detected in the fractionated samples than in the unfractionated samples, and 17% were enhanced. The improvement was especially prominent in the case of HMW proteins. Well-separated HMW proteins were analyzed by MALDI-TOF mass spectrometry. The molecular masses of the identified proteins in the > 48 kDa gel segment were distributed between 50 and 112 kDa, thus validating this prefractionation method. Identified HMW proteins with similar mass but different pI were mostly isoforms. Thus SDS-PAGE-based size prefractionation provides improved separation and detection of HMW proteins.