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We recently developed a novel hyperspectral excitation-resolved near-infrared fluorescence imaging system (HER-NIRF) based on a continuous-wave wavelength-swept laser. In this study, this technique is applied to measure the distribution of the therapeutic agent dimethyl sulfoxide (DMSO) by utilizing solvatochromic shift in the spectral profile of albumin-bound Indocyanine green (ICG). Using wide-field imaging in turbid media, complex dynamics of albumin-bound ICG are measured in mixtures of dimethyl sulfoxide (DMSO) and water. Phantom experiments are conducted to evaluate the performance of the HER-NIRF system. The results show that the distribution of DMSO can be visualized in the wide-field reflection geometry. One of the main purposes of the DMSO is to act as a carrier for other drugs, enhancing their effects by facilitating skin penetration. Understanding the solubility and permeability of drugs in vivo is very important in drug discovery and development. Hence, this HER-NIRF technique has great potential to advance the utilization of the therapeutic agent DMSO by mapping its distribution via the solvatochromic shift of ICG. By customizing the operational wavelength range, this system can be applied to any other fluorophores in the near-infrared region and utilized for a wide variety of drug delivery studies.

Diffuse optical tomography (DOT) has been studied for use in the detection of breast cancer, cerebral oxygenation, and cognitive brain signals. As optical imaging studies have increased significantly, acquiring imaging data in real time has become increasingly important. We have developed frequency-division multiplexing (FDM) DOT systems to analyze their performance with respect to acquisition time and imaging quality, in comparison with the conventional time-division multiplexing (TDM) DOT. A large tomographic area of a cylindrical phantom 60 mm in diameter could be successfully reconstructed using both TDM DOT and FDM DOT systems. In our experiment with 6 source-detector (S-D) pairs, the TDM DOT and FDM DOT systems required 6.18 and 1 s, respectively, to obtain a single tomographic data set. While the absorption coefficient of the reconstruction image was underestimated in the case of the FDM DOT, we experimentally confirmed that the abnormal region can be clearly distinguished from the background phantom using both methods.

We present a nanosecond Q-switched Erbium-doped fiber (EDF) laser system operating at 1600 nm with a tunable repetition rate from 100 kHz to 1 MHz. A compact fiber coupled, acousto-optic modulator-based EDF ring cavity was used to generate a nanosecond seed laser at 1600 nm, and a double-cladding EDF based power amplifier was applied to achieve the maximum average power of 250 mW. In addition, 12 ns laser pulses with the maximum pulse energy of 2.4 μJ were obtained at 100 kHz. Furthermore, the Stokes shift by Raman scattering over a 25 km long fiber was measured, indicating that the laser can be potentially used to generate the high repetition rate pulses at the 1.7 μm region. Finally, we detected the photoacoustic signal from a human hair at 200 kHz repetition rate with a pulse energy of 1.2 μJ, which demonstrates that a Q-switched Er-doped fiber laser can be a promising light source for the high speed functional photoacoustic imaging.

Analysing dynamics of a single biomolecule using high-resolution imaging techniques has been had significant attentions to understand complex biological system. Among the many approaches, vertical nanopillar arrays in contact with the inside of cells have been reported as a one of useful imaging applications since an observation volume can be confined down to few-tens nanometre theoretically. However, the nanopillars experimentally are not able to obtain super-resolution imaging because their evanescent waves generate a high optical loss and a low signal-to-noise ratio. Also, conventional nanopillars have a limitation to yield 3D information because they do not concern field localization in z-axis. Here, we developed novel hybrid nanopillar arrays (HNPs) that consist of SiO2 nanopillars terminated with gold nanodisks, allowing extreme light localization. The electromagnetic field profiles of HNPs are obtained through simulations and imaging resolution of cell membrane and biomolecules in living cells are tested using one-photon and 3D multiphoton fluorescence microscopy, respectively. Consequently, HNPs present approximately 25 times enhanced intensity compared to controls and obtained an axial and lateral resolution of 110 and 210 nm of the intensities of fluorophores conjugated with biomolecules transported in living cells. These structures can be a great platform to analyse complex intracellular environment.

Intravascular photoacoustic imaging at 1.7 μm spectral band has shown promising capabilities for lipid-rich vulnerable atherosclerotic plaque detection. In this work, we report a high speed catheter-based integrated intravascular photoacoustic/intravascular ultrasound (IVPA/IVUS) imaging system with a 500 Hz optical parametric oscillator laser at 1725 nm. A lipid-mimicking phantom and atherosclerotic rabbit abdominal aorta were imaged at 1 frame per second, which is two orders of magnitude faster than previously reported in IVPA imaging with the same wavelength. Clear photoacoustic signals by the absorption of lipid rich deposition demonstrated the ability of the system for high speed vulnerable atherosclerotic plaques detection.

We developed an optical frequency domain imaging (OFDI) system based on an integrated thermo-optic swept laser to achieve three-dimensional surface imaging. The wavelength was swept by applying a heating signal to a thermo-optic polymeric waveguide. The sub-micrometer surface profile was converted from the three-dimensional phase information of the OFDI system on various samples used as resolution targets with a step height of 120 nm. © 2014 IOP Publishing Ltd.

We report a simultaneous dual-band wavelength-swept laser based on the active mode locking method. By applying a single modulation signal, synchronized sweeping of two lasing-wavelengths is demonstrated without the use of a mechanical wavelength-selecting filter. Two free spectral ranges are independently controlled with a dual path-length configuration of a laser cavity. The static and dynamic performances of a dual-band wavelength-swept active mode locking fiber laser are characterized in both the time and wavelength regions. Two lasing wavelengths were swept simultaneously from 1263.0 to 1333.3 nm for the 1310 nm band and from 1493 to 1563.3 nm for the 1550 nm band. The application of a dual-band wavelength-swept fiber laser was also demonstrated with a dual-band optical coherence tomography imaging system. © 1989-2012 IEEE.

Decommissioning and closure of Canada's Underground Research Laboratory (URL) involved installing a full-scale 5 m diameter by 12 m long seal in the main access shaft. The seal consisted of clay and concrete components spanning a major water-bearing fracture zone (FZ2) located at a depth of around 275 m that isolates two hydrogeochemical regimes. This paper presents results of coupled hydromechanical (H-M) numerical modelling that simulated measured performance of the seal. Parameters for the H-M modeling were determined from previously measured laboratory test data. Results from the modeling were compared with field data for water uptake and total pressures in the system. They show that hydration of the clay seal was dominated by the groundwater flow from FZ2. The simulations showed good agreement with field readings and provide an improved understanding of various interrelated physical processes that take place during hydration. Key words: shaft seal, hydromechanical (H-M) performance, swelling pressure, soil suction, soil water retention curve. La desaffectation et la fermeture du laboratoire souterrain de recherche (LRS) du Canada impliquaient l'installation d'un scellement a pleine echelle de 5 m de diametre sur 12 m de long dans le puits d'acces principal. Le scellement consistait en des composants d'argile et de beton couvrant une zone de fracture aquifere majeure (FZ2) situee a une profondeur de 275 m qui isole deux regimes hydrogeochimiques. Cet article presente les resultats d'une modelisation numerique couplee hydro-mecanique (H-M) qui simule les performances mesurees du scellement. Les parametres pour la modelisation H-M ont ete determines a partir de donnees prealablement mesurees lors d'essais de laboratoire. Les resultats de la modelisation ont ete compares aux donnees de terrain pour l'absorption d'eau et les pressions totales dans le systeme. Ils montrent que l'hydratation du scellement d'argile etait dominee par le flux d'eau souterraine provenant de FZ2. Les simulations ont montre une bonne concordance avec les lectures sur le terrain et ont permis de mieux comprendre les differents processus physiques interdepen-dants qui se produisent pendant l'hydratation. Mots-cles : scellement de puits, performance hydromecanique (H-M), pression de gonflement, succion du sol, courbe de retention d'eau., 1. Introduction During closure of the Underground Research Laboratory (URL) in south-eastern Manitoba, Atomic Energy of Canada Limited (AECL) constructed a composite concrete-clay-concrete seal in the main access shaft to [...]

We present an ultrathin fiber-optic endoscopy probe for optical coherence tomography (OCT), which is made of a series of fused optical fibers instead of the conventional scheme based on an objective lens. The large-core fiber with a core diameter of 20 μm was utilized for the probe, while a single-mode fiber of core diameter 8.2 μm mainly delivered the OCT light. Those fibers were spliced with a bridge fiber of an intermediate core size. The guided light was stepwise converted to a beam of a large mode-field diameter to be radiated with a larger depth of focus. We obtained a 125 μm thick all-fiber endoscopy probe with a side-viewing capability implemented by an angled fiber end. Successful OCT imaging was demonstrated with a swept-source OCT system and showed the practical applicability of our lens-free endoscopy probe.

A novel linearized interrogation method is presented for a Fourier domain mode-locked (FDML) fiber Bragg grating (FBG) sensor system. In a high speed regime over several tens of kHz modulations, a sinusoidal wave is available to scan the center wavelength of an FDML wavelength-swept laser, instead of a conventional triangular wave. However, sinusoidal wave modulation suffers from an exaggerated non-uniform wavelength-spacing response in demodulating the time-encoded parameter to the absolute wavelength. In this work, the calibration signal from a polarization-maintaining fiber Sagnac interferometer shares the FDML wavelength-swept laser for FBG sensors to convert the time-encoded FBG signal to the wavelength-encoded uniform-spacing signal.

This paper shows experimentally dual wavelength swept at multi band (O,C-Band) property with active mode locking (AML) method. Unlike conventional wavelength swept laser, AML wavelength swept laser does not require any wavelength selecting filter in the cavity. The cavity has two free spectral ranges (FSRs) depend on dual path configuration. This wavelength swept laser can be useful for wide-band fiber-optic application such as Optical Coherence Tomography and Fiber Bragg Grating sensor system. © 2012 SPIE.

This paper shows experimentally dual wavelength swept at multi band (O, C-Band) property with active mode locking (AML) method. Unlike conventional wavelength swept laser, AML wavelength swept laser does not require any wavelength selecting filter in the cavity. The cavity has two free spectral ranges (FSRs) depend on dual path configuration. This wavelength swept laser can be useful for wi de-band fiber-optic application. © 2012 IEEE.

A flexible curled optical cord is useful for a common-path optical coherence tomography (OCT) system because a bending-insensitive arbitrary length can be chosen for the endoscopic imaging probe. However, there has been a critical problem that the partial reflector needs to be placed in between the sample and the objective lens. It limits the structure design of optical probe and leads to a low transverse resolution OCT imaging. Instead of a conventional single common-path interferometer, we propose a novel double common-path interferometer configuration in order to generate an interference signal that is independent of the optical distance between the partial reflector and sample. Due to the limitless tuning of the objective distance, an objective lens with a high numerical aperture (NA) up to 0.85 can be successfully used for phase-sensitive optical coherence tomography to achieve a 3-dimensional profile image of a transverse resolution of 0.7 μm. The intensity and phase terms of the interference signal can be obtained simultaneously from a Fourier-domain mode locked swept laser source for fast data acquisition with a phase stability of 979 pm.

We present a calibration protocol to obtain the alignment factors of a custom-made spectrometer and the nonlinear fitting function between the measured CCD pixel domain and the wavelength domain to apply to the spectral-domain optical coherence tomography (SD-OCT) using fiber Bragg gratings. We have used five gratings with different center wavelengths covering the broadband source spectral range. All have a narrow spectral bandwidth (0.05 nm) and the same reflectivity (92%) to calibrate and align the custom-made spectrometer. The implemented SD-OCT system following the proposed protocol showed the alignment factors as 44.37 deg incident angle, 53.11 deg diffraction angle, and 70.0-mm focal length. The spectral resolution of 0.187 nm was recalculated from the alignment factors.

In this study, we develop an ultra-fast fiber Bragg grating sensor system that is based on the Fourier domain mode-locked (FDML) swept laser. A FDML wavelength swept laser has many advantages compared to the conventional wavelength swept laser source, such as high-speed interrogation, narrow spectral sensitivity, and high phase stability. The newly developed FDML wavelength swept laser shows a superior performance of a high scan rate of 31.3 kHz and a broad scan range of over 70 nm simultaneously. The performance of the grating sensor interrogating system using a FDML wavelength swept laser is characterized in both static and dynamic strain responses. (C) 2008 Optical Society of America