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2. Proliferation and DNA ploidy in malignant breast tumors in relation to early oral contraceptive use and early abortions

Author

Olsson, H., Ranstam, J., Baldetorp, B., Ewers, S.-B., Ferno, M., Killander, D., and Sigurdsson, H.

Subjects
Breast cancer -- Risk factors, Cell proliferation -- Health aspects, Miscarriage -- Complications, Abortion -- Complications, Oral contraceptives -- Adverse and side effects, Ploidy -- Health aspects, Abortion -- Adverse and side effects, Health
Abstract

For many years there has been some concern that the hormones used in oral contraceptives might contribute to an increase in the risk of breast cancer. Research has failed to confirm an overall association between birth control pills and breast cancer. However, in recent years it has become clear that, for specific subgroups of women, oral contraceptives do impose an increase in risk. These subgroups include women who started using oral contraceptives at an early age and used them for an extended period, women who used birth control pills for an extended period prior to the birth of their first child, and childless women who have used oral contraceptives for an extended period. Research has also demonstrated that women who developed premenopausal breast cancer after using oral contraceptives at an early age tended to have larger tumors at diagnosis and were more likely to have lymph node metastases. These observations suggest that oral contraceptive use is associated with more aggressive cancers. For this reason, investigators examined some of the cellular characteristics of breast cancers in a group of 175 women with premenopausal breast cancer to determine if features thought to be associated with greater cancer aggressiveness differed in the women with a history of oral contraceptive use. Measuring the S-phase fraction, which is the fraction of cells undergoing DNA synthesis at a given time, it was found that women who used oral contraceptives before the age of 20 tended to be higher in this measure of cell proliferation activity. This increase in S-phase fraction was evident in aneuploid tumors, which have an abnormal chromosome complement, but not in diploid tumors. Furthermore, aneuploid tumors were also more common in this group. It was also found that the S-phase fraction was 49 percent higher among women who had early abortions. Previous research has shown that abortions and miscarriages increase the risk of breast cancer; the present research shows that they also are associated with markers of cancers of increased aggressiveness. While the precise mechanisms by which oral contraceptive use or abortions affect the development of breast cancer are not known, one possibility is that since the normal proliferation of breast tissue is greatest in the teenage years, factors which influence breast tissue during this time may result in more aggressive tumors. (Consumer Summary produced by Reliance Medical Information, Inc.)

Published
1991

4. Studies on Antibody Formation in Patients Treated with L-Asparaginase

Author

Dohlwitz, A., Franzén, S., Holmgren, A., Killander, A., Killander, D., Wide, L., Åhström, L., Allfrey, V. G., editor, Allgöwer, M., editor, Bauer, K. H., editor, Berenblum, I., editor, Bergel, F., editor, Bernard, J., editor, Bernhard, W., editor, Blokhin, N. N., editor, Bock, H. E., editor, Bucalossi, P., editor, Chaklin, A. V., editor, Chorazy, M., editor, Cunningham, G. J., editor, Dameshek, W., editor, Dargent, M., editor, Della Porta, G., editor, Denoix, P., editor, Dulbecco, R., editor, Eagle, H., editor, Eker, R., editor, Grabar, P., editor, Hamperl, H., editor, Harris, R. J. C., editor, Hecker, E., editor, Herbeuval, R., editor, Higginson, J., editor, Hueper, W. C., editor, Isliker, H., editor, Karnofsky, D. A., editor, Kieler, J., editor, Klein, G., editor, Koprowski, H., editor, Koss, L. G., editor, Martz, G., editor, Mathé, G., editor, Mühlbock, O., editor, Nakahara, W., editor, Potter, V. R., editor, Sabin, A. B., editor, Sachs, L., editor, Saxén, E. A., editor, Spiegelmann, S., editor, Szybalski, W., editor, Tagnon, H., editor, Taylor, R. M., editor, Tissières, A., editor, Uehlinger, E., editor, Wissler, R. W., editor, Yoshida, T., editor, Rentchnick, P., editor, Grundmann, E., editor, and Oettgen, H. F., editor

Published
1970
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6. Third International Congress of Histochemistry and Cytochemistry

Author

Abe, Muneaki, Akamatsu, Masasuke, Matsumoto, Takaharu, Ohuchi, Nobuo, Masuya, Tomichi, Abe, T., Nakashio, K., Kazama, M., Matsuda, M., Adams, C. W. M., Virág, S., Morgan, R. S., Orton, C. C., Adams, Jean R., Wilcox, Theodore A., Akert, Konrad, Alberti, Rachele, Allen, Robert C., Moore, Dorothy J., Tyndall, Richard L., Anderson, Paul J., Song, Sun K., Angelakos, E. T., King, M. P., Appleton, Timothy C., Arstila, Antti U., Trump, Benjamin F., Lauria, A., Bahr, Gunter F., Wied, George L., Bartels, Peter H., Bajusz, E., Balogh, Kåroly, Barer, R., Barka, Tibor, Barnard, Eric A., Komender, Janusz, Wieckowski, Jan, Barrnett, R. J., Barron, K. D., Koeppen, A. H., Bernsohn, J., Bélanger, Leonard F., Beltrami, Carlo Alberto, Björklund, A., Ehinger, B., Falck, B., Boadle, Margaret C., Bloom, Floyd E., Bona, C., Bradshaw, M., Monus, L., Stroman, S., Budd, G. C., Salpeter, M. M., Bukhonova, A. I., Burnasheva, S. A., Jurzina, G. A., Burt, Alvin M., Chang, Jeffrey P., Schatzki, Peter F., Saito, Takuma, Chavin, Walter, Chyle, M., Korych, B., Lojda, Zdenek, Patocka, F., Cohn, Z. A., Conning, D. M., Coutinho, Hélio B., Rocha, Jácia T., Jales, Benjamin F., Cunningham, Lew, Heitsch, Richard, Daneholt, B., Edström, J.-E., Danilova, L. V., Rokhlenko, K. D., Dauwalder, M., Whaley, W. G., Kephart, J. E., Deitch, Arline D., Sawicki, Stanley G., Godman, Gabriel C., Della Corte, Francesco, Desmet, V. J., Bullens, A.-M., De Groote, J., Heirwegh, K. P. M., Diculescu, I., Onicescu, Doina, Szegly, G., Doane, Winifred W., Donskikh, N. V., Novikov, V. D., Subbotin, M. Ya., Tsirelnikov, N. I., Doolin, Paul F., Birge, Wesley J., Droz, Bernard, Bergeron, M., Drukker, J., Duarte-Escalante, Ovidio, Dubowitz, Victor, Dupraw, E. J., Eckner, Friedrich A. O., Blackstone, Eugene H., Moulder, Peter V., Ehrlich, M. P., Ellis, Stanley, McDonald, J. Ken, Callahan, P. X., Enesco, Hildegard E., Engel, W. King, Epifanova, O. I., Lomakina, L. Ya., Terskikh, V. V., Ericsson, Jan L. E., Jakobsson, Sten, Eristawi, K. D., Sharashidze, L. K., Sturua, N. S., Fabris, Guidalberto, Mariuzzi, Gianmario, Nenci, Italo, Fahimi, H. Dariush, Karnovsky, Morris J., Fand, Sally B., Farquhar, Marilyn G., Felgenhauer, K., Glenner, G. G., Stammler, A., Filkuka, J., Svejda, J., Áubrechtova, V., Filotto, U., Fischbein, J. W., Rutenburg, Alexander M., Fisher, Donald B., Forni, Alessandra, Nencioni, Torquato, Ballare’, Gianfranco, Fotin, Ludmila, Popescu, Maria, Frankfurt, O. S., Friend, Daniel S., Fuchs, B. B., Arutyunov, V. D., Shnaper, A. L., Gabunia, U. A., Shiukashvili, N. N., Gahan, P. B., Anker, P., Stroun, M., McLean, Jean, Galjaard, H., Bootsma, D., Ganina, K. P., Garcia, Alfredo Mariano, Garrett, J. R., Gepts, W., Gregoire, F., Ooms, H., Gerzeli, Giuseppe, Giacobini, Ezio, Hovmark, Stefan, Gilkerson, Seth W., Glick, David, Godlewski, H. G., Huszczuk, A., Penar, Barbara, Goldfischer, Sidney, Sternlieb, Irmin, Goldstone, A., Szabo, E., Koenig, Harold, Gornak, K. A., Goslar, H. G., Grigoriadis, P., Jaeger, K. H., Gössner, W., Benoit, H., Gracheva, Nina D., Grillo, T. Adesanya Ige, Gropp, A., Gross, U. M., Gueft, Boris, Guha, S., Fouquet, J. P., Håkanson, R., Owman, Ch., Sporrong, B., Hale, A. J., Marshall, D. J., Switsur, V. R., Hanker, Jacob S., Zenker, Nicolas, Morizono, Yoshihisa, Deb, Chandicharan, Seligman, Arnold M., Hardonk, M. J., Elema, J. D., Koudstaal, Joh., Hoedemaeker, Ph. J., Hayashi, Masando, Heller, A., Hernández, F., Martinez De Morentin, J., Herrmann, Hans-Jürgen, Hershey, Falls B., Hess, H. H., Pope, A., Bass, N. H., Hewitt, J. M., Guigon, M., Bolubasz, J., Himes, M. H., Burdick, C., Hirai, Kei-Ichi, Takamatsu, Hideo, Hirose, Shunta, Hirsch, Hilde E., Hodges, Donald R., Costoff, Allen, McShan, W. H., Holtzman, Eric, Holubar, K., Tappeiner, J., Wolff, K., Hopsu-Havu, Väinö K., Hosannah, Yvonne, Blackwood, Carlton E., Mandl, Ines, Hoskins, Godfrey C., Hugon, J. S., Borgers, M., Hurwitz, Lawrence S., Rubinstein, Lucien J., Ibrahim, M. Z. M., Imura, Shin-Ichi, Takeda, Masanori, Jacobsen, N. O., Jørgensen, P. Leth, Jarrett, A., Joandrea-Casian, Claudia, Prundeanu, Cornelia, Johnson, Anne B., Johnson, Waine C., Alkek, David S., Jongkind, J. F., Swaab, D. F., Jos, J., Junqueira, L. C., Toledo, A. M. Souza, Kaiser, Hans E., Kakari, Sophia, Kalina, Moshe, Bubis, Jose. J., Kamentsky, L. A., Kasten, Frederick H., Kiefer, Gunter G., Sandritter, W., Killander, D., Rigler, R., Kishino, Yasuo, Kobayashi, H., Urano, A., Yokoyama, K., Koelle, George B., Hughes, Charles, Korhonen, L. Kalevi, Kramer, M. F., Poort, C., Kreutzberg, Georg W., Künzel, Erich, Tanyolac, Attila, Labella, Frank S., Langley, O. K., Lanza, Giovanni B., Lappano-Colletta, Eleanor Rita, Leblond, C. P., Merzel, J., Cheng, Hazel, Nadler, N. J., Herscovics, Annette A., Lederer, B., Mittermayer, C., Lee, Sin Hang, Torack, Richard M., Lehrer, Gerard M., Bornstein, Murray B., Katzman, Robert, Leites, F. L., Tendetnik, Ju. J., Ruchadse, E. S., Rjadneva, O. E., Leppi, T. John, Kinnison, Patricia A., Gaffney, Susan P., Lev, Robert, Gerard, Andre, De Graef, Jacques, Jerzy Glass, George B., Lhotka, J. F., Jr., Anderson, J. W., Liber, Amour F., Lillie, R. D., Pizzolato, Philip, Lindner, J., Grasedyck, K., Johannes, G., Freytag, G., Lipchina, L. P., Aksyutina, M. S., Yablonovskaya, L. Ya., Lipetz, Jacques, Liu, J. C., Roizin, L., Lodin, Z., Kage, M., Hartman, J., Srajer, J., Fric, Premysl, Long, Margaret E., Sommers, Sheldon C., McGarry, E. E., Nayak, R., Birch, E., Beck, J. C., McMillan, Paul J., Adeoye, Christopher ’Seinde, Macovschi, O., Maeda, Ryuei, Ihara, Nobuo, Kanazawa, Kokichi, Maeir, David M., Wagner, Lenore, Maggi, Viviane, Franks, L. M., Livingston, D. C., Coombs, M. M., Wilson, Patricia D., Carbonell, A. W., Malyuk, V. I., Romanini, Manfredi, Gabriella, Maria, Fraschini, Annunzia, Porcelli, Franca, Manocha, Sohan L., Shantha, Totada R., Bourne, Geoffrey H., Marques, Dante, Bastos, A. L., Baptista, A. M., Vigario, J. D., Nunes, J. M., Terrinha, A. M., Silva, J. A. F., Masurovsky, E. B., Benitez, H. H., Kim, S-U., Murray, M. R., Matschinsky, F. M., Rutherford, C. L., Guerra, L., Matturri, L., Curri, S., Melnick, P. J., Mendelsohn, M. L., Conway, T. J., Perry, B., Prewitt, J. M. S., Mercado, Teresa I., Miksche, Jerome P., Misch, Donald W., Misch, Margaret S., Mitchell, J. P., Mizuhira, Vinci, Uchida, Kazuko, Amakawa, Takanori, Shindo, Hideo, Totsu, Junichi, Suesada, Ikuo, Mizutani, Akira, Monis, Benito, Candiotti, Alberto, Mori, G., Ingrami, A., Morikawa, Shigeru, Yamamura, Masao, Harada, Takayuki, Hamashima, Yoshihiro, Mullaney, P. F., Dean, P. N., Van Dilla, M. A., Müller, Gerhard, Müller, Otfried, Nakane, Paul K., Neurath, Peter W., Curtis, Zay B., Selles, William, Vetter, Henri G., Norgren, P. E., Novikoff, Alex B., O’Brien, Regina, Ohringer, Philip, Spitaleri, Vincent, Olszewska, M. J., Gabara, B., Konopska, L., Parfanovich, M. I., Sokolov, N. N., Berezina, O. N., Fadeeva, L. L., Pauly, John E., Scheving, Lawrence E., Pearse, A. G. E., Pearson, Bjarne, Bennett, William, Esterly, John R., Standen, Alfred C., Pelc, S. R., Viola-Magni, M. P., Penttilä, Antti, Perez, Vernon J., Moore, Blake W., Peters, Theodore, Jr., Danzi, J. Thomas, Ashley, Charles A., Petrova, A. S., Probatova, N. A., Philippens, Karel, Pilgrim, C., Pollock, B. M., Presnov, M. A., Preston, Kendall, Jr., Preto Parvis, V., Cisotti, F., Prewitt, Judith M. S., Mayall, Brian H., Mendelsohn, Mortimer L., Pryse-Davis, John, Sandler, Merton, Quay, W. B., Raikhlin, N. T., Rasch, Ellen M., Riecken, E. O., Goebell, H., Bode, C., Rigatuso, Joseph L., Ringertz, N. R., Bolund, L., Ritter, Carl, Thorell, Bo, Rizzotti, M., Aureli, G., Balduini, C., Castellani, A. A., Rosenbaum, Robert M., Rosene, Gordon L., Rossi, Ferdinando, Rost, F. W. D., Roth, Daniel, Ruch, Fritz, Ruddle, Frank H., Lubs, Herbert A., Ledley, Robert S., Shows, Thomas B., Roderick, Thomas H., Kim, H., Brodie, E., Rosales, C. L., Sadauskas, P., Luksys, L., Dabkevcius, V., Sakharova, A. V., Sakharov, D. A., Samosudova, N. V., Ogieveckaja, M. M., Kalamkarova, M. B., Sandler, Maurice, Santti, R. S., Sasaki, Mitsuo, Takeuchi, Tadao, Satir, P., Schauer, Alfred, Scher, Stanley, Haley, Patricia L., Schiebler, T. H., Schiemer, Hans-Georg, Schlüns, Jürgen, Schuster, F. L., Hershenov, B., Scott, J. E., Scott, T. Gilbert, Seno, Satimaru, Yokomura, Ei-Ichi, Itoh, Nobutaka, Yamamoto, Michio, Shungskaya, V. E., Enenko, S. O., Lukyanova, L. D., Sarch, E. N., Shuter, Eli, Jungalwala, Firoze, Robins, Eli, Sierakowska, Halina, Silverman, L., Simard, A., Daoust, R., Smith, Edgar E., Smith, R. E., Fishman, William H., Henzl, Milan, Sobel, Harold J., Avrin, Erna, Sorokin, Helen P., Sorokin, Sergei, Squier, C. A., Waterhouse, J. P., Steinbach, Günter, Steplewski, Zenon, Stitnimankarn, Tinrat, Stoward, Peter J., Straus, W., Stumpf, Walter E., Roth, Lloyd J., Sylvén, B., Kanamura, Shinsuke, Templeton, McCormick, Tewari, H. B., Tyagi, H. R., Thalmann, R., Glismann, L., Thomas, E., Tice, Lois W., Tixier-Vidal, A., Törö, I., Bacsy, E., Vadasz, Gy., Rappay, Gy., Tsou, K. C., Chang, Mildred Y., Matsukawa, S., Goodwin, Cleon, Lynm, Dwo, Seamond, Bette, Van Der Ploeg, M., Van Duijn, P., Coulter, J. R., Pascoe, E., Van Fleet, D. S., VanHouten, Wiecher H., Vecher, A. S., Masko, A. A., Predkel, K. I., Reshetnikov, V. N., Tchaika, M. T., Velican, C., Velican, Doina, Vendrely, C., Lageron, A., Tournier, P., Vialli, Maffo, Prenna, Giovanni, Vilter, Voldemar, Vittek, Josef, Vogt, Arnold, Vollrath, L., Von Mayersbach, Heinz, Vorbrodt, Andrzej, Wächtler, Klaus, Wakabayashi, Katsumi, Tamaoki, Bun-Ichi, Wald, Niel, Ranshaw, Russell, Weller, Roy O., Welsch, Ulrich, Werner, Gottfried, Williams, Vick, Morriss, Fran, Willighagen, R. G. J., Wilson, Barry W., Wohlrab, Frank, Wolf, Paul L., Horwitz, Jerome P., Freisler, Josef V., Von Der Muehll, Elisabeth, Vazquez, Janice, Wolman, Moshe, Yamada, Masaoki, Iwata, Sunao, Yamaguchi, Hisao, Yataganas, X., Gahrton, G., Young, Ian T., Zaccheo, D., Grossi, C. E., Genta, V., Riva, A., Zacks, S. I., Sheff, M. F., Zamfirescu-Gheorghiu, M., Serban, M., Vladescu, C., Chirulescu, Z., Marcus, N., Zelenin, A. V., Kirianova, E. A., Stepanova, N. G., Zeuthen, Erik, Zimmermann, Horst, Zugibe, Frederick T., Abrahamson, Dean E., Anderson, Norman G., Caspersson, Törbjorn, Cornell, Richard, Dougherty, William, Jirasek, J. E., Jonsson, Gösta, Leske, Regina, Moyer, Frank H., Schneider, Walter C., Siegel, Howard I., Sternberger, Ludwig, Osserman, Elliott F., Weinstock, A., and Rosenbaum, Robert M.

Published
1968
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7. Adjuvant immunotherapy in acute nonlymphocytic leukemia

Author

Lindemalm, Ch. S-n, Killander, A., Björkholm, M., Brenning, G., Engstedt, L., Franzén, S., Gahrton, G., Gullbring, B., Holm, G., Höglund, S., Hörnsten, P., Jameson, S., Killander, D., Klein, E., Lantz, B., Lockner, D., Lönnqvist, B., Mellstedt, H., Palmblad, J., Pauli, C., Reizenstein, P., Simonsson, B., Skårberg, K. -O., Udén, A. -M., Vanky, F., and Wadman, B.

Published
1978
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8. TRPA1 mediates spinal antinociception induced by acetaminophen and the cannabinoid Delta(9)-tetrahydrocannabiorcol

Author

Andersson, D. (D) A. (A), Gentry, C. (C), Alenmyr, L. (L), Killander, D. (D), Lewis, S. (S) E. (E), Andersson, A. (A), Bucher, B. (Bernard), Galzi, J. (Jean-Luc) L. (L), Sterner, O. (O), Bevan, S. (S), Hogestatt, E. (E) D. (D), and Zygmunt, P. (P) M. (M)

Subjects
digestive, oral, and skin physiology, Aucun, food and beverages, psychological phenomena and processes
Abstract

TRPA1 is a unique sensor of noxious stimuli and, hence, a potential drug target for analgesics. Here we show that the antinociceptive effects of spinal and systemic administration of acetaminophen (paracetamol) are lost in Trpa1(-/-) mice. The electrophilic metabolites N-acetyl-p-benzoquinoneimine and p-benzoquinone, but not acetaminophen itself, activate mouse and human TRPA1. These metabolites also activate native TRPA1 and, as a consequence, sensory neurons. The N-acetyl-p-benzoquinoneimine metabolite L-cysteinyl-S-acetaminophen was detected in the mouse spinal cord after systemic acetaminophen administration. In the hot-plate test, intrathecal administration of N-acetyl-p-benzoquinoneimine, p-benzoquinone and the electrophilic TRPA1 activator cinnamaldehyde Trpa1(-/-) mice. Intrathecal injection of a non-electrophilic cannabinoid, Delta(9)-tetrahydrocannabiorcol, also produced TRPA1-dependent antinociception in this test. Our study provides a molecular mechanism for the antinociceptive effect of acetaminophen and discloses spinal TRPA1 activation as a potential pharmacological strategy to alleviate pain.

Published
2011

12. Third International Congress of Histochemistry and Cytochemistry

Author

Muneaki, Abe, primary, Akamatsu, Masasuke, additional, Matsumoto, Takaharu, additional, Ohuchi, Nobuo, additional, Masuya, Tomichi, additional, Abe, T., additional, Nakashio, K., additional, Kazama, M., additional, Matsuda, M., additional, Adams, C. W. M., additional, Virág, S., additional, Morgan, R. S., additional, Orton, C. C., additional, Adams, Jean R., additional, Wilcox, Theodore A., additional, Akert, Konrad, additional, Alberti, Rachele, additional, Allen, Robert C., additional, Moore, Dorothy J., additional, Tyndall, Richard L., additional, Anderson, Paul J., additional, Song, Sun K., additional, Angelakos, E. T., additional, King, M. P., additional, Appleton, Timothy C., additional, Arstila, Antti U., additional, Trump, Benjamin F., additional, Aureli, G., additional, Lauria, A., additional, Rizzotti, M., additional, Bahr, G. F., additional, Wied, G. L., additional, Bartels, P., additional, Bajusz, E., additional, Barer, R., additional, Barka, Tibor, additional, Barnard, Eric A., additional, Komender, Janusz, additional, Wieckowski, Jan, additional, Barrnett, R. J., additional, Barron, K. D., additional, Koeppen, A. H., additional, Bernsohn, J., additional, Bélanger, Leonard F., additional, Beltrami, Carlo Alberto, additional, Björklund, A., additional, Ehinger, B., additional, Falck, B., additional, Boadle, Margaret C., additional, Bloom, Floyd E., additional, Bona, C., additional, Bradshaw, M., additional, Stroman, S., additional, Monus, L., additional, Budd, G. C., additional, Salpeter, M. M., additional, Bukhonova, A. I., additional, Burnasheva, S. A., additional, Jurzina, G. A., additional, Burt, Alvin M., additional, Chang, Jeffre Y. P., additional, Schatzki, Peter F., additional, Saito, Takuma, additional, Chavin, Walter, additional, Chyle, M., additional, Korych, B., additional, Lojda, Z., additional, Patocka, F., additional, Cohn, Z. A., additional, Conning, D. M., additional, Coutinho, Hélio B., additional, Rocha, Jácia T., additional, Jales, Benjamin F., additional, Cunningham, Lew, additional, Heitsch, Richard, additional, Daneholt, B., additional, Edström, J.-E., additional, Danilova, L. V., additional, Rokhlenko, K. D., additional, Dauwalder, M., additional, Whaley, W. G., additional, Kephart, J. E., additional, Deitch, Arline D., additional, Sawicki, Stanley G., additional, Godman, Gabriel C., additional, Francesco, Della Corte, additional, Desmet, V. J., additional, Bullens, A.-M., additional, De Groote, J., additional, Heirwegh, K. P. M., additional, Diculescu, I., additional, Onicescu, Doina, additional, Szegly, G., additional, Doane, Winifred W., additional, Donskikh, N. V., additional, Novikov, V. D., additional, Subbotin, M. Ya., additional, Tsirelnikov, N. I., additional, Doolin, Paul F., additional, Birge, Wesley J., additional, Bernard, Droz, additional, Droz, B., additional, Bergeron, M., additional, Drukker, J., additional, Duarte-Escalante, Ovidio, additional, Dubowitz, Victor, additional, Dupraw, E. J., additional, Eckner, Friedrich A. O., additional, Blackstone, Eugene H., additional, Moulder, Peter V., additional, Ehrlich, M. P., additional, Stanley, Ellis, additional, McDonald, J. Ken, additional, Callahan, P. X., additional, Epifanova, O. I., additional, Lomakina, L. Ya., additional, Terskikh, V. V., additional, Ericsson, Jan L. E., additional, Jakobsson, Sten, additional, Eristawi, K. D., additional, Sharashidze, L. K., additional, Sturua, N. S., additional, Fabris, G., additional, Mariuzzi, G. M., additional, Nenci, I., additional, Fahimi, Dariush H., additional, Karnovsky, Morris J., additional, Fand, Sally B., additional, Farquhar, Marilyn G., additional, Felgenhauer, K., additional, Glenner, G. G., additional, Stammler, A., additional, Filkuka, J., additional, Svejda, J., additional, Áubrechtova, V., additional, Filotto, U., additional, Fischbein, J. W., additional, Rutenburg, A. M., additional, Fisher, Donald B., additional, Alessandra, Forni, additional, Nencioni, Torquato, additional, Ballare, Gianfranco, additional, Ludmila, Fotin, additional, Popescu, Maria, additional, Frankfurt, O. S., additional, Friend, Daniel S., additional, Fuchs, B. B., additional, Arutyunov, V. D., additional, Shnaper, A. L., additional, Gabunia, U. A., additional, Shiukashvili, N. N., additional, Gahan, P. B., additional, Anker, P., additional, Stroun, M., additional, McLean, Jean, additional, Galjaard, H., additional, Bootsma, D., additional, Ganina, K. P., additional, Garcia, Alfredo Mariano, additional, Garrett, J. R., additional, Gepts, W., additional, Gregoire, F., additional, Ooms, H., additional, Giuseppe, Gerzeli, additional, Ezio, Giacobini, additional, Hovmark, Stefan, additional, Gilkerson, Seth W., additional, David, Glick, additional, Godlewski, H. G., additional, Huszczuk, A., additional, Penar, Barbara, additional, Goldfischer, Sidney, additional, Sternlieb, Irmin, additional, Goldstone, A., additional, Szabo, E., additional, Koenig, H., additional, Gornak, K. A., additional, Goslar, H. G., additional, Grigoriadis, P., additional, Jaeger, K. H., additional, Gössner, W., additional, Benoit, H., additional, Gracheva, Nina D., additional, Grillo, T. Adesanya Ige, additional, Gropp, A., additional, Gross, U. M., additional, Gueft, Boris, additional, Guha, S., additional, Fouquet, J. P., additional, Håkanson, R., additional, Owman, Ch., additional, Sporrong, B., additional, Hale, A. J., additional, Marshall, D. J., additional, Switsur, V. R., additional, Hanker, Jacob S., additional, Zenker, Nicolas, additional, Morizono, Yoshihisa, additional, Deb, Chandicharan, additional, Seligman, Arnold M., additional, Hardonk, M. J., additional, Elema, J. D., additional, Koudstaal, Joh, additional, Hoedemaeker, Ph. J., additional, Hayashi, Masando, additional, Heller, A., additional, Hernández, F., additional, De Morentin, J. Martinez, additional, Hans-Jürgen, Herrmann, additional, Hershey, Falls B., additional, Hess, H. H., additional, Pope, A., additional, Bass, N. H., additional, Hewitt, J. M., additional, Guigon, M., additional, Bolubasz, J., additional, Himes, M. H., additional, Burdick, C., additional, Hirai, Kei-Ichi, additional, Takamatsu, Hideo, additional, Shunta, Hirose, additional, Hirsch, Hilde E., additional, Hodges, Donald R., additional, Costoff, Allen, additional, McShan, W. H., additional, Holtzman, Eric, additional, Holubar, K., additional, Tappeiner, J., additional, Wolff, K., additional, Hopsu-Havu, Vainö K., additional, Hosannah, Yvonne, additional, Blackwood, Carlton E., additional, Mandl, Ines, additional, Hoskins, Godfrey C., additional, Hugon, J. S., additional, Borgers, M., additional, Hurwitz, Lawrence S., additional, Rubinstein, Lucien J., additional, Ibrahim, M. Z. M., additional, Imura, Shin-Ichi, additional, Takeda, Masanori, additional, Jacobsen, N. O., additional, Jørgensen, P. Leth, additional, Jarrett, A., additional, Claudia, Joandrea-Casian, additional, Prundeanu, Cornelia, additional, Johnson, Anne B., additional, Johnson, Waine C., additional, Alkek, David S., additional, Jongkind, J. F., additional, Swaab, D. F., additional, Jos, J., additional, Junqueira, L. C., additional, Toledo, A. M. Souza, additional, Kaiser, Hans E., additional, Kakari, Sophia, additional, Kalina, Moshe, additional, Bubis, Jose. J., additional, Kamentsky, L. A., additional, Kasten, Frederick H., additional, Kiefer, Gunter G., additional, Sandritter, W., additional, Killander, D., additional, Rigler, R., additional, Yasuo, Kishino, additional, Kobayashi, H., additional, Urano, A., additional, Yokoyama, K., additional, Koelle, George B., additional, Koenig, Harold, additional, Hughes, Charles, additional, Korhonen, Kalevi L., additional, Kramer, M. F., additional, Poort, C., additional, Kreutzberg, Georg W., additional, Künzel, Erich, additional, Tanyolac, Attila, additional, Labella, Frank S., additional, Langley, O. K., additional, Lanza, Giovanni B., additional, Lappano-Colletta, Eleanor Rita, additional, Leblond, C. P., additional, Merzel, J., additional, Cheng, Hazel, additional, Nadler, N. J., additional, Herscovics, Annette A., additional, Lederer, B., additional, Mittermayer, C., additional, Lee, Sin Hang, additional, Torack, Richard M., additional, Lehrer, Gerard M., additional, Bornstein, Murray B., additional, Katzman, Robert, additional, Leites, F. L., additional, Tendetnik, Ju. J., additional, Ruchadse, E. S., additional, Rjadneva, O. E., additional, Leppi, T. John, additional, Kinnison, Patricia A., additional, Gaffney, Susan P., additional, Lev, Robert, additional, Gerard, Andre, additional, de Graef, Jacques, additional, Glass, George B. Jerzy, additional, Lhotka, J. F., additional, Anderson, J. W., additional, Liber, Amour F., additional, Lillie, R. D., additional, Pizzolato, Philip, additional, Lindner, J., additional, Grasedyck, K., additional, Johannes, G., additional, Freytag, G., additional, Gries, G., additional, Lipchina, L. P., additional, Aksyutina, M. S., additional, Yablonovskaya, L. Ya., additional, Lipetz, Jacques, additional, Liu, J. C., additional, Roizin, L., additional, Lodin, Z., additional, Kage, M., additional, Hartman, J., additional, Srajer, J., additional, Lojda, Zdenek, additional, Fric, Premysl, additional, Long, Margaret E., additional, Sommers, Sheldon C., additional, Ken, McDonald J., additional, Ellis, Stanley, additional, McGarry, E. E., additional, Nayak, R., additional, Birch, E., additional, Beck, J. C., additional, McMillan, Paul J., additional, Adeoye, Christopher ’Seinde, additional, Macovschi, O., additional, Maeda, Ryuei, additional, Ihara, Nobuo, additional, Kanazawa, Kokichi, additional, Maeir, David M., additional, Wagner, Lenore, additional, Viviane, Maggi, additional, Franks, L. M., additional, Livingston, D. C., additional, Coombs, M. M., additional, Wilson, Patricia D., additional, Carbonell, A. W., additional, Malyuk, V. I., additional, Romanini, Manfredi, additional, Gabriella, Maria, additional, Fraschini, Annunzia, additional, Porcelli, Franca, additional, Manocha, Sohan L., additional, Shantha, Totada R., additional, Bourne, Geoffrey H., additional, Marques, Dante, additional, Bastos, A. L., additional, Baptista, A. M., additional, Vigario, J. D., additional, Nunes, J. M., additional, Terrinha, A. M., additional, Silva, J. A. F., additional, Masurovsky, E. B., additional, Benitez, H. H., additional, Kim, S-U., additional, Murray, M. R., additional, Matschinsky, F. M., additional, Rutherford, C. L., additional, Guerra, L., additional, Matturri, L., additional, Curri, S., additional, Mayall, Brian H., additional, Melnick, P. J., additional, Mendelsohn, M. L., additional, Conway, T. J., additional, Perry, B., additional, Prewitt, J. M. S., additional, Mercado, Teresa I., additional, Miksche, Jerome P., additional, Misch, Donald W., additional, Misch, Margaret S., additional, Mitchell, J. P., additional, Kiefer, G., additional, Mizuhira, Vinci, additional, Uchida, Kazuko, additional, Amakawa, Takanori, additional, Shindo, Hideo, additional, Totsu, Junichi, additional, Suesada, Ikuo, additional, Mizutani, Akira, additional, Monis, Benito, additional, Candiotti, Alberto, additional, Mori, G., additional, Ingrami, A., additional, Morikawa, Shigeru, additional, Yamamura, Masao, additional, Harada, Takayuki, additional, Hamashima, Yoshihiro, additional, Mullaney, P. F., additional, Dean, P. N., additional, Van Dilla, M. A., additional, Müller, Gerhard, additional, Müller, Otfried, additional, Nakane, Paul K., additional, Neurath, Peter W., additional, Curtis, Zay B., additional, Selles, William, additional, Vetter, Henri G., additional, Norgren, P. E., additional, Novikoff, Alex B., additional, Regina, O‘brien, additional, Ohringer, Philip, additional, Spitaleri, Vincent, additional, Olszewska, M. J., additional, Gabara, B., additional, Konopska, L., additional, Parfanovich, M. I., additional, Sokolov, N. N., additional, Berezina, O. N., additional, Fadeeva, L. L., additional, Pauly, John E., additional, Scheving, Lawrence E., additional, Pearse, A. G. E., additional, Pearson, Bjarne, additional, Bennett, William, additional, Esterly, John R., additional, Standen, Alfred C., additional, Pelc, S. R., additional, Viola-Magni, M. P., additional, Antti, Penttilä, additional, Perez, Vernon J., additional, Moore, Blake W., additional, Peters, Theodore, additional, Danzi, J. Thomas, additional, Ashley, Charles A., additional, Petrova, A. S., additional, Probatova, N. A., additional, Philippens, Karel, additional, Pilgrim, C., additional, Pollock, B. M., additional, Presnov, M. A., additional, Preston, Kendall, additional, Preto, V. Parvis, additional, Cisotti, F., additional, Mazza, G. E., additional, Prewitt, Judith M. S., additional, Mendelsohn, Mortimer L., additional, Pryse-Davis, John, additional, Sandler, Merton, additional, Quay, W. B., additional, Raikhlin, N. T., additional, Rasch, Ellen M., additional, Riecken, E. O., additional, Goebell, H., additional, Bode, C., additional, Rigatuso, Joseph L., additional, Ringertz, N. R., additional, Bolund, L., additional, Carl, Ritter, additional, Thorell, Bo, additional, Balduini, C., additional, Castellani, A. A., additional, Rosenbaum, Robert M., additional, Rosene, Gordon L., additional, Rossi, Ferdinando, additional, Rost, F. W. D., additional, Roth, Daniel, additional, Ruch, Fritz, additional, Ruddle, Frank H., additional, Lubs, Herbert A., additional, Ledley, Robert S., additional, Shows, Thomas B., additional, Roderick, Thomas H., additional, Kim, H., additional, Brodie, E., additional, Fischbein, J., additional, Rosales, C. L., additional, Sadauskas, P., additional, Luksys, L., additional, Dabkevcius, V., additional, Sakharova, A. V., additional, Sakharov, D. A., additional, Samosudova, N. V., additional, Ogieveckaja, M. M., additional, Kalamkarova, M. B., additional, Sandler, Maurice, additional, Santti, R. S., additional, Hopsu-Havu, V. K., additional, Sasaki, Mitsuo, additional, Takeuchi, Tadao, additional, Satir, P., additional, Schauer, Alfred, additional, Scher, Stanley, additional, Haley, Patricia L., additional, Schiebler, T. H., additional, Schiemer, Hans-Georg, additional, Schlüns, Jürgen, additional, Schuster, F. L., additional, Hershenov, B., additional, Scott, J. E., additional, Scott, T. Gilbert, additional, Seno, Satimaru, additional, Yokomura, Ei-ichi, additional, Itoh, Nobutaka, additional, Yamamoto, Michio, additional, Shungskaya, V. E., additional, Enenko, S. O., additional, Lukyanova, L. D., additional, Sarch, E. N., additional, Eli, Shuter, additional, Jungalwala, Firoze, additional, Robins, Eli, additional, Slerakowska, Halina, additional, Silverman, L., additional, Glick, D., additional, Simard, A., additional, Daoust, R., additional, Smith, Edgar E., additional, Smith, R. E., additional, Fishman, William H., additional, Henzl, Milan, additional, Sobel, Harold J., additional, Avrin, Erna, additional, Sorokin, Helen P., additional, Sorokin, Sergei, additional, Sorokin, Sergei P., additional, Squier, C. A., additional, Waterhouse, J. P., additional, Steinbach, Günter, additional, Steplewski, Zenon, additional, Stitnimankarn, Tinrat, additional, Stoward, Peter J., additional, Straus, W., additional, Stumpf, Walter E., additional, Roth, Lloyd J., additional, Sylvèn, B., additional, Kanamura, Shinsuke, additional, Templeton, McCormick, additional, Tewari, H. B., additional, Tyagi, H. R., additional, Thalmann, R., additional, Glismann, L., additional, Thomas, E., additional, Tice, Lois W., additional, Tixier-Vidal, A., additional, Törö, I., additional, Bacsy, E., additional, Vadasz, Gy., additional, Rappay, Gy., additional, Tsou, K. C., additional, Chang, Mildred Y., additional, Matsukawa, S., additional, Goodwin, Cleon, additional, Lynm, Dwo, additional, Seamond, Bette, additional, Van der Ploeg, M., additional, Van Duijn, P., additional, Coulter, J. R., additional, Pascoe, E., additional, Van Fleet, D. S., additional, Van Houten, Wiecher H., additional, Vecher, A. S., additional, Masko, A. A., additional, Predkel, K. I., additional, Reshetnikov, V. N., additional, Tchaika, M. T., additional, Velican, C., additional, Velican, Doina, additional, Vendrely, C., additional, Lageron, A., additional, Tournier, P., additional, Vialli, Maffo, additional, Prenna, Giovanni, additional, Vilter, Voldemar, additional, Vittek, Josef, additional, Vogt, Arnold, additional, Vollrath, L., additional, Von Mayersbach, Heinz, additional, Andrzej, Vorbrodt, additional, Wächtler, Klaus, additional, Wakabayashi, Katsumi, additional, Bun-Ichis, Tamaoki, additional, Niel, Wald, additional, Ranshaw, Russell, additional, Weller, Roy O., additional, Welsch, Ulrich, additional, Werner, Gottfried, additional, Vick, Williams, additional, Morriss, Fran, additional, Willighagen, R. G. J., additional, Wilson, Barry W., additional, Wohlrab, Frank, additional, Wolf, Paul L., additional, Horwitz, Jerome P., additional, Freisler, Josef V., additional, Von der Muehll, Elisabeth, additional, Vazquez, Janice, additional, Wolman, Moshe, additional, Wolman, M., additional, Kalina, M., additional, Bubis, J. J., additional, Yamada, Masaoki, additional, Iwata, Sunao, additional, Yamaguchi, Hisao, additional, Yataganas, X., additional, Gahrton, G., additional, Thorell, B., additional, Young, Ian T., additional, Zaccheo, D., additional, Grossi, C. E., additional, Genta, V., additional, Riva, A., additional, Zacks, S. I., additional, Sheff, M. F., additional, Zamfirescu-Gheorghiu, M., additional, Serban, M., additional, Vladescu, C., additional, Chirulescu, Z., additional, Marcus, N., additional, Zelenin, A. V., additional, Kirianova, E. A., additional, Stepanova, N. G., additional, Zeuthen, Erik, additional, Zimmermann, Horst, additional, Zugibe, Frederick T., additional, Abrahamson, Dean E., additional, Anderson, Norman G., additional, Caspersson, Törbjorn, additional, Richard, Cornell, additional, Dougherty, William, additional, Jirasek, J. E., additional, Jonsson, Gösta, additional, Leske, Regina, additional, Moyer, Frank H., additional, Schneider, Walter C., additional, Siegel, Howard I., additional, Sternberger, Ludwig, additional, Osserman, Elliott F., additional, Weinstock, A., additional, Kåroly, Balogh, additional, and Enesco, Hildegard E., additional

Published
1968
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14. Optimizing flow cytometric DNA ploidy and S-phase fraction as independent prognostic markers for node-negative breast cancer specimens

Author

Bagwell, CB, Clark, GM, Spyratos, F, Chassevent, A, Bendahl, PO, Stål, Olle, Killander, D, Jourdan, ML, Romain, S, Hunsberger, B, Baldetorp, B, Bagwell, CB, Clark, GM, Spyratos, F, Chassevent, A, Bendahl, PO, Stål, Olle, Killander, D, Jourdan, ML, Romain, S, Hunsberger, B, and Baldetorp, B

Abstract

Developing a reliable and quantitative assessment of the potential virulence of a malignancy has been a long-standing goal in clinical cytometry. DNA histogram analysis provides valuable information on the cycling activity of a tumor population through S-phase estimates; it also identifies nondiploid populations, a possible indicator of genetic instability and subsequent predisposition to metastasis. Because of conflicting studies in the literature, the clinical relevance of both of these potential prognostic markers has been questioned for the management of breast cancer patients. The purposes of this study are to present a set of 10 adjustments derived from a single large study that optimizes the prognostic strength of both DNA ploidy and S-phase and to test the validity of this approach on two other large multicenter studies. Ten adjustments to both DNA ploidy and S-phase were developed from a single node-negative breast cancer database from Baylor College (n = 961 cases). Seven of the adjustments were used to reclassify histograms into low-risk and high-risk ploidy patterns based on aneuploid fraction and DNA index optimum thresholds resulting in prognostic P values changing from little (P < 0.02) or no significance to P < 0.000005. Other databases from Sweden (n = 210 cases) and France (n = 220 cases) demonstrated similar improvement of DNA ploidy prognostic significance, P < 0.02 to P < 0.0009 and P < 0.12 to P < 0.002, respectively. Three other adjustments were applied to diploid and aneuploid S-phases. These adjustments eliminated a spurious correlation between DNA ploidy and S-phase and enabled them to combine independently into a powerful prognostic model capable of stratifying patients into low, intermediate, and high-risk groups (P < 0.000005). When the Baylor prognostic model was applied to the Sweden and French databases, similar significant patient stratifications were observed (P < 0.0003 and P < 0.00001, respectively). The

Published
2001
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15. DNA and cell cycle analysis as prognostic indicators in breast tumors revisited

Author

Bagwell, CB, Clark, GM, Chassevent, A, Bendahl, PO, Stål, Olle, Killander, D, Jourdan, ML, Romain, S, Hunsberger, B, Wright, S, Baldetorp, B, Bagwell, CB, Clark, GM, Chassevent, A, Bendahl, PO, Stål, Olle, Killander, D, Jourdan, ML, Romain, S, Hunsberger, B, Wright, S, and Baldetorp, B

Abstract

Both DNA ploidy and S-phase ploidy are promising prognostic factors for node-negative breast cancer patients. Based largely on the analysis of one large study, much of the reported problems with these factors have been caused by some unappreciated complexities in categorizing DNA ploidy into low- and high-risk groups and the lack of some necessary adjustments to eliminate unwanted correlations between DNA S-phase and ploidy. When both DNA ploidy and S-phase are compensated properly, they become independent prognostic markers, forming a powerful prognostic model.

Published
2001

16. Multivariate analyses of flow cytometric S-phase and ploidy as node-negative breast cancer prognostic factors : an international and multi-center study

Author

Bagwell, CB, Clark, GM, Spyratos, F, Chassevent, A, Bendahl, PO, Stål, Olle, Killander, D, Jourdan, ML, Romain, S, Hunsberger, CB, Wright, S, Baldetorp, B, Bagwell, CB, Clark, GM, Spyratos, F, Chassevent, A, Bendahl, PO, Stål, Olle, Killander, D, Jourdan, ML, Romain, S, Hunsberger, CB, Wright, S, and Baldetorp, B

Abstract

Recently a set of ten adjustments that optimizes the prognostic strength of both DNA ploidy (P) and S-phase (S) was published (Cytometry, 46(3), 2001). Also presented was an optimal method of combining P and S (P+S) that stratifies node-negative patients into highly significant risk groups. The adjustments compensate for many unappreciated complexities in categorizing P into low and high risk groups and eliminate unwanted correlation between P and S. The purpose of this study is to examine P+S in the context of other well-known prognostic factors such as primary size (pT), estrogen and progesterone receptor (ER,PR) and menopausal status (MS). Methods: DNA histograms derived from frozen primary tumors and clinical databases were provided by Baylor College, n=935; Sweden, n=210 (Lund, Linkoping, Stockholm) and France, n=220 (Angers, Marseille, Saint Cloud, Tours). Time to metastasis was the tested clinical outcome. Results: Cox proportional hazards analysis of theBaylor data revealed P+S, p<0.000002, and pT, p<0.003, as independent significant prognostic factors. The Sweden study also showed P+S the mostsignificant prognostic factor, p<0.002, as well as MS, p<0.004 and ER, p<0.007. The French study results were MS, p<0.0005, P+S, p<0.002 and pT, p<0.007.A P+S, MS and pT prognostic model stratified patients in all studies into highly significant categories, Baylor, p<0.000005, Sweden, p<0.00001, and French, p<0.000005, with low and high risk 10-year relapse-free survival fractions of 0.92-0.69, 0.95-0.58 and 0.96-0.60 respectively. Conclusion: A combined P+S, MS and pT prognostic model is a powerful and reliable method of stratifying node-negative breast cancer patients into highly significant prognostic groups.

Published
2001
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18. Prognostic significance of p53 overexpression in primary breast cancer : a novel luminometric immunoassay applicable on steroid receptor cytosols.

Author

Borg, A, Lennerstrand, Johan, Stenmark-Askmalm, M, Fernö, M, Brisfors, A, Ohrvik, A, Stål, O, Killander, D, Lane, D, Brundell, J, Borg, A, Lennerstrand, Johan, Stenmark-Askmalm, M, Fernö, M, Brisfors, A, Ohrvik, A, Stål, O, Killander, D, Lane, D, and Brundell, J

Abstract

A novel quantitative luminometric immunoassay (LIA) has been developed for the measurement of wild-type and mutant p53 protein in extracts from breast tumour tissue. The LIA was found to yield reliable estimates of p53 expression in cytosol samples routinely prepared for steroid receptor analysis as compared with results obtained with immunohistochemical analysis. The LIA was evaluated on 205 primary breast tumour cytosols prepared for steroid receptor analysis and stored frozen at -80 degrees C for 6-8 years, p53 protein being detected in 65% of the samples (range 0.01-23 ng mg-1 protein). Using an arbitrary cut-off value of 0.15 ng mg-1 protein, 30% of the tumours were classified as manifesting p53 overexpression. Significant and independent correlations were found to exist between p53 overexpression and shorter disease-free (P < 0.001) and overall survival (P = 0.039) at a median duration of follow-up of 50 months. p53 overexpression was related to low oestrogen receptor content and high proliferation rate (S-phase fraction). No relationship was found to tumour size or the presence of lymph node metastasis. Three tumours possessed an extremely high p53 content (> 10 ng mg-1 protein), all of which were of medullary or high-grade ductal type, oestrogen and progesterone receptor negative, DNA non-diploid, had S-phase fractions of > 22% and recurred within 1-2 years. In summary, a new sensitive and quantitative LIA suitable for routine analysis of p53 protein in steroid receptor cytosol preparations from breast tumours has been developed to confirm the prognostic importance of p53 protein accumulation in human breast cancer.

Published
1995
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34. CVP-remission-maintenance in stage I or II non-Hodgkin's lymphomas: preliminary results of a randomized study.

Author

Landberg, Torsten G., Håkansson, Leif G., Möller, Torgil R., Mattsson, Willy K. I., Landys, Karol E., Johansson, Bo G., Killander, Dick C. F., Molin, Bjö Rn F., Westling, Per F., Lenner, Per H., Dahl, Olov G., Landberg, T G, Håkansson, L G, Möller, T R, Mattsson, W K, Landys, K E, Johansson, B G, Killander, D C, Molin, B F, and Westling, P F

Published
1979
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38. Short-Term Tissue Culture of Two Nonsecretory Human Myelomas.

Author

Nilsson, K., Killander, D., Killander, J., and Mellstedt, H.

Subjects
TISSUE culture, MONOCLONAL antibodies, SERUM, MYELOMA proteins, BONE marrow cells, ENDOPLASMIC reticulum, ORGANELLES
Abstract

Bone marrow cells from two patients without detectable monoclonal immunoglobulin (Ig) in serum and urine but with the clinical picture of plasma cell myeloma were cultivated in vitro. Immunofluorescence studies of cultured living and fixed bone marrow cells showed no signs of Ig production in one of the cases, whereas in the other case cytoplasmic kappa chains were detected, which, however, were not expressed at the surface of living cells. Cells from the latter patient were also subjected to kinetic, ultrastructural, and functional studies in vitro. The fraction of myeloma cells incorporating tritiated thymidine in vitro decreased gradually during prolonged culture, indicating a continuous cell death. The morphological characterization revealed many similarities between this nonsecretory myeloma and classical myelomas, although the frequency of cells with cisternae of endoplasmic reticulum distended by a granular material was unusually high, as was the frequency of ‘flaming’ myeloma cells. ‘Flaming’ cells were not labeled by tritiated thymidine, suggesting that they are nonproliferative end cells. Studies of the Ig synthesis by gel diffusion analyses of supernatant and cell lysates from [14C]leucine-labeled cultures agreed with the immunofluorescence studies that the myeloma cells were nonsecretory. [ABSTRACT FROM AUTHOR]

Published
1976
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43. Quantification of Immunofluorescence on Individual Erythrocytes Coated with Varying Amounts of Antigen.

Author

Killander, D., Levin, A., Inoue, Masaharu, and Klein, Eva

Subjects
*ANTIGEN-antibody reactions, *IMMUNOFLUORESCENCE, *IMMUNOGLOBULIN M, *ANTIGENS, *ERYTHROCYTES, *PROTEINS
Abstract

Quantification of the parameters in an antigen-antibody fluorescent reaction in which it was possible to vary the amount of the antigen, was carried out in a model system. Formalinized erythrocytes were coated with different amounts of 131I-labelled human IgM. The cells were then exposed to fluorescein-conjugated antiserum to human IgM. The fluorescence intensity of individual cells was measured with a microspectrofluorimeter. As the cells were coated with varying amounts of protein it was possible to investigate the relationship between the quantity of protein antigen and the intensity of fluorescence. [ABSTRACT FROM AUTHOR]

Published
1970

50. 3H-Thymidine Autoradiography and Cytophotometric Analysis of Needle Aspirates from Human Tumours During Radiation Therapy, Endocrine Therapy and Chemotherapy

Author

Klintenberg, C., Bjelkenkrantz, K., Månsson, J. -C., Killander, D., and Nordenskjöld, B.

Abstract

Cell kinetic variables in human turnours were investigated before and during therapy. Tumour cell material was collected by sequential thin needle aspiration biopsy. Small variations in DNA replication could be detected by 3H-thymidine autoradiography. In tumours with a high rate of replication, therapy induced changes could also be detected with flow cytometry and static cytophotometry. Endocrine treatment with glucocorticoids, radiation therapy, and chemotherapy were associated with reduced rates of DNA replication in malignant lymphomas. The conformity between labelling index and S-phase estimates from DNA histograms was poorer in biopsy specimens collected during therapy than in pretreatment specimens.

Published
1985
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