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28 results on '"Kholodilov Ivan S."'

1. Dermacentor reticulatus – a tick on its way from glacial refugia to a panmictic Eurasian population

Author

Bilbija, Branka, Spitzweg, Cäcilia, Papoušek, Ivo, Fritz, Uwe, Földvári, Gábor, Mullett, Martin, Ihlow, Flora, Sprong, Hein, Civáňová Křížová, Kristína, Anisimov, Nikolay, Belova, Oxana A., Bonnet, Sarah I., Bychkova, Elizabeth, Czułowska, Aleksandra, Duscher, Georg G., Fonville, Manoj, Kahl, Olaf, Karbowiak, Grzegorz, Kholodilov, Ivan S., Kiewra, Dorota, Krčmar, Stjepan, Kumisbek, Gulzina, Livanova, Natalya, Majláth, Igor, Manfredi, Maria Teresa, Mihalca, Andrei D., Miró, Guadalupe, Moutailler, Sara, Nebogatkin, Igor V., Tomanović, Snežana, Vatansever, Zati, Yakovich, Marya, Zanzani, Sergio, and Široký, Pavel

Published
2023
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2. An Inactivated West Nile Virus Vaccine Candidate Based on the Lineage 2 Strain.

Author

Vorovitch, Mikhail F., Tuchynskaya, Ksenia K., Kruglov, Yuriy A., Peunkov, Nikita S., Mostipanova, Guzal F., Kholodilov, Ivan S., Ivanova, Alla L., Fedina, Maria P., Gmyl, Larissa V., Morozkin, Evgeny S., Roev, German V., Karan, Lyudmila S., and Karganova, Galina G.

Subjects
WEST Nile fever, WEST Nile virus, TICK-borne encephalitis, VIRAL vaccines, VACCINE immunogenicity
Abstract

Background: West Nile virus (WNV) is a rapidly growing problem worldwide. The lack of emergency treatment and a safe licensed vaccine against WNV allows the virus to cause sporadic outbreaks of human disease, including fatal cases. Formalin-inactivated vaccines have been used for a long time and have been shown to be very safe and effective, especially in susceptible populations. Methods: By adapting tick-borne encephalitis vaccine production technology, we produced a laboratory-inactivated vaccine against WNV based on the strain SHUA, isolated from humans with a lethal WNV infection in the year 2021. Results: The potential vaccine was tested for safety in vitro and in vivo in outbred SHK mice of different ages, including PCR analysis of the brains of these mice to test for the absence of viral RNA after intracerebral injection. Conclusions: The inactivated whole-virion laboratory vaccine showed 100% seroconversion and immunogenicity against WNV strain SHUA-1, isolated from a lethal human case, and provided the mice with 100% protection from disease and death. [ABSTRACT FROM AUTHOR]

Published
2024
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4. Phlebovirus sequences detected in ticks collected in Russia: Novel phleboviruses, distinguishing criteria and high tick specificity

Author

Klimentov, Alexander S., Belova, Oxana A., Kholodilov, Ivan S., Butenko, Alexander M., Bespyatova, Liubov A., Bugmyrin, Sergey V., Chernetsov, Nikita, Ivannikova, Anna Y., Kovalchuk, Irina V., Nafeev, Alexander A., Oorzhak, Natalia D., Pilikova, Olga M., Polienko, Alexandra E., Purmak, Kristina A., Romanenko, Evgeniya N., Romanova, Lidiya Iu., Saryglar, Anna A., Solomashchenko, Nataliya I., Shamsutdinov, Anton F., Vakalova, Elena V., Lukashev, Alexander N., Karganova, Galina G., and Gmyl, Anatoly P.

Published
2020
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8. Virome of the tabanids from Russia

Author

Litov, Alexander G., primary, Belova, Oxana A., additional, Kholodilov, Ivan S., additional, Kalyanova, Anna S., additional, Gadzhikurbanov, Magomed N., additional, Rogova, Anastasia A., additional, Gmyl, Larissa V., additional, and Karganova, Galina G., additional

Published
2023
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11. Viromes of Tabanids from Russia.

Author

Litov, Alexander G., Belova, Oxana A., Kholodilov, Ivan S., Kalyanova, Anna S., Gadzhikurbanov, Magomed N., Rogova, Anastasia A., Gmyl, Larissa V., and Karganova, Galina G.

Subjects
MAMMALIAN embryos, HORSEFLIES, NUCLEOTIDE sequencing, CELL culture, TICKS, VIRAL genomes
Abstract

Advances in sequencing technologies and bioinformatics have greatly enhanced our knowledge of virus biodiversity. Currently, the viromes of hematophagous invertebrates, such as mosquitoes and ixodid ticks, are being actively studied. Tabanidae (Diptera) are a widespread family, with members mostly known for their persistent hematophagous behavior. They transmit viral, bacterial, and other pathogens, both biologically and mechanically. However, tabanid viromes remain severely understudied. In this study, we used high-throughput sequencing to describe the viromes of several species in the Hybomitra, Tabanus, Chrysops, and Haematopota genera, which were collected in two distant parts of Russia: the Primorye Territory and Ryazan Region. We assembled fourteen full coding genomes of novel viruses, four partial coding genomes, as well as several fragmented viral sequences, which presumably belong to another twelve new viruses. All the discovered viruses were tested for their ability to replicate in mammalian porcine embryo kidney (PEK), tick HAE/CTVM8, and mosquito C6/36 cell lines. In total, 16 viruses were detected in at least one cell culture after three passages (for PEK and C6/36) or 3 weeks of persistence in HAE/CTVM8. However, in the majority of cases, qPCR showed a decline in virus load over time. [ABSTRACT FROM AUTHOR]

Published
2023
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12. Tetrahydroquinazoline N ‐oxide derivatives inhibit reproduction of tick‐borne and mosquito‐borne flaviviruses

Author

Sedenkova, Kseniya N., primary, Uvarova, Victoria I., additional, Nazarova, Anna A., additional, Peisikova, Aleksandra V., additional, Khvatov, Evgeny V., additional, Sukhorukov, Maksim V., additional, Frolenko, Vasilisa S., additional, Goryashchenko, Alexander S., additional, Kholodilov, Ivan S., additional, Grishin, Yuri K., additional, Rybakov, Viktor B., additional, Makenov, Marat T., additional, Morozkin, Evgeny S., additional, Karan, Lyudmila S., additional, Kozlovskaya, Liubov I., additional, Ishmukhametov, Aydar A., additional, Osolodkin, Dmitry I., additional, and Averina, Elena B., additional

Published
2023
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13. Marburg virus in Egyptian Rousettus bats in Guinea: Investigation of Marburg virus outbreak origin in 2021

Author

Makenov, Marat T., primary, Boumbaly, Sanaba, additional, Tolno, Faya Raphael, additional, Sacko, Noumouny, additional, N’Fatoma, Leno Tamba, additional, Mansare, Oumar, additional, Kolie, Bonaventure, additional, Stukolova, Olga A., additional, Morozkin, Evgeny S., additional, Kholodilov, Ivan S., additional, Zhurenkova, Olga B., additional, Fyodorova, Marina V., additional, Akimkin, Vasily G., additional, Popova, Anna Yu., additional, Conde, Namoudou, additional, Boiro, Mamadou Yero, additional, and Karan, Lyudmila S., additional

Published
2023
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15. Integrated Jingmenvirus Polymerase Gene in Ixodes ricinus Genome

Author

Morozkin, Evgeny S., primary, Makenov, Marat T., additional, Zhurenkova, Olga B., additional, Kholodilov, Ivan S., additional, Belova, Oxana A., additional, Radyuk, Ekaterina V., additional, Fyodorova, Marina V., additional, Grigoreva, Yana E., additional, Litov, Alexander G., additional, Valdokhina, Anna V., additional, Bulanenko, Victoria P., additional, Samoilov, Andrei E., additional, Korneenko, Elena V., additional, Voizekhovskaya, Yana A., additional, Neverov, Alexey D., additional, Karganova, Galina G., additional, and Karan, Lyudmila S., additional

Published
2022
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17. Distribution and Characterisation of Tick-Borne Flavi-, Flavi-like, and Phenuiviruses in the Chelyabinsk Region of Russia.

Author

Kholodilov, Ivan S., Belova, Oxana A., Ivannikova, Anna Y., Gadzhikurbanov, Magomed N., Makenov, Marat T., Yakovlev, Alexander S., Polienko, Alexandra E., Dereventsova, Alena V., Litov, Alexander G., Gmyl, Larissa V., Okhezin, Egor V., Luchinina, Svetlana V., Klimentov, Alexander S., and Karganova, Galina G.

Subjects
*TICK-borne encephalitis viruses, *DERMACENTOR
Abstract

In this work, we presented data from a two-year study of flavi-, flavi-like, and phenuiviruses circulation in the population of ixodid ticks in the Chelyabinsk region. We isolated three tick-borne encephalitis virus (TBEV) strains from I. persulcatus, which was not detected in the ticks of the genus Dermacentor. The virus prevalence ranged from 0.66% to 2.28%. The Yanggou tick virus (YGTV) is widespread in steppe and forest-steppe zones and is mainly associated with ticks of the genus Dermacentor. We isolated 26 strains from D. reticulatus, D. marginatus, and I. persulcatus ticks in the HAE/CTVM8 tick cell line. The virus prevalence ranged from 1.58% to 4.18% in D. reticulatus, ranged from 0.78% to 3.93% in D. marginatus, and was 0.66% in I. persulcatus. There was combined focus of TBEV and YGTV in the territory of the Chelyabinsk region. The Alongshan virus (ALSV) was found to be associated with I. persulcatus ticks and is spread in forest zone. We detected 12 amplicons and isolated 7 strains of ALSV in tick cells. The virus prevalence ranged from 1.13% to 6.00%. The phlebovirus Gomselga and unclassified phenuivirus Stavropol were associated with I. persulcatus and D. reticulatus ticks, respectively. Virus prevalence of the unclassified phenuivirus Stavropol in the Chelyabinsk region is lower than that in neighbouring regions. [ABSTRACT FROM AUTHOR]

Published
2022
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18. Possible Arbovirus Found in Virome of Melophagus ovinus

Author

Litov, Alexander G., primary, Belova, Oxana A., additional, Kholodilov, Ivan S., additional, Gadzhikurbanov, Magomed N., additional, Gmyl, Larissa V., additional, Oorzhak, Natalia D., additional, Saryglar, Anna A., additional, Ishmukhametov, Aydar A., additional, and Karganova, Galina G., additional

Published
2021
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19. Geographical and Tick-Dependent Distribution of Flavi-Like Alongshan and Yanggou Tick Viruses in Russia

Author

Kholodilov, Ivan S., primary, Belova, Oxana A., additional, Morozkin, Evgeny S., additional, Litov, Alexander G., additional, Ivannikova, Anna Y., additional, Makenov, Marat T., additional, Shchetinin, Alexey M., additional, Aibulatov, Sergey V., additional, Bazarova, Galina K., additional, Bell-Sakyi, Lesley, additional, Bespyatova, Liubov A., additional, Bugmyrin, Sergey V., additional, Chernetsov, Nikita, additional, Chernokhaeva, Liubov L., additional, Gmyl, Larissa V., additional, Khaisarova, Anna N., additional, Khalin, Alexei V., additional, Klimentov, Alexander S., additional, Kovalchuk, Irina V., additional, Luchinina, Svetlana V., additional, Medvedev, Sergey G., additional, Nafeev, Alexander A., additional, Oorzhak, Natalia D., additional, Panjukova, Elena V., additional, Polienko, Alexandra E., additional, Purmak, Kristina A., additional, Romanenko, Evgeniya N., additional, Rozhdestvenskiy, Evgeniy N., additional, Saryglar, Anna A., additional, Shamsutdinov, Anton F., additional, Solomashchenko, Nataliya I., additional, Trifonov, Vladimir A., additional, Volchev, Evgenii G., additional, Vovkotech, Pavel G., additional, Yakovlev, Alexander S., additional, Zhurenkova, Olga B., additional, Gushchin, Vladimir A., additional, Karan, Lyudmila S., additional, and Karganova, Galina G., additional

Published
2021
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21. Isolation and Characterisation of Alongshan Virus in Russia

Author

Kholodilov, Ivan S., primary, Litov, Alexander G., additional, Klimentov, Alexander S., additional, Belova, Oxana A., additional, Polienko, Alexandra E., additional, Nikitin, Nikolai A., additional, Shchetinin, Alexey M., additional, Ivannikova, Anna Y., additional, Bell-Sakyi, Lesley, additional, Yakovlev, Alexander S., additional, Bugmyrin, Sergey V., additional, Bespyatova, Liubov A., additional, Gmyl, Larissa V., additional, Luchinina, Svetlana V., additional, Gmyl, Anatoly P., additional, Gushchin, Vladimir A., additional, and Karganova, Galina G., additional

Published
2020
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23. First isolation and characterisation of Alongshan virus in Russia

Author

Kholodilov, Ivan S., primary, Litov, Alexander G., additional, Klimentov, Alexander S., additional, Belova, Oxana A., additional, Polienko, Alexandra E., additional, Nikitin, Nikolai A., additional, Shchetinin, Alexey M., additional, Ivannikova, Anna Y., additional, Bell-Sakyi, Lesley, additional, Yakovlev, Alexander S., additional, Bugmyrin, Sergey V., additional, Bespyatova, Liubov A., additional, Gmyl, Larissa V., additional, Luchinina, Svetlana V., additional, Gmyl, Anatoly P., additional, Gushchin, Vladimir A., additional, and Karganova, Galina G., additional

Published
2019
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24. The use of microarrays for the identification of the origin of genes of avian influenza viruses in wild birds

Author

Heydarov Rustam N., Lomakina Natalia F., Boravleva Elizaveta Yu., Kholodilov Ivan S., Gambaryan Alexandra S., Mikhailovich Vladimir M., and Fesenko Eugene E.

Subjects
animal structures, viruses, virus diseases, avian influenza virus, monitoring, microarray
Abstract

Forty-two strains of avian influenza viruses were isolated from the wild waterfowls’ feces in the city of Moscow. These viruses, as well as reference strains and some experimental reassortants, were analyzed by microarrays. The microarrays contained 176 probes to the different segments of influenza virus genome. The microarray helps to determine 1) the hemagglutinin and neuraminidase proteins subtype; 2) the primary structure of the C-terminal sequence of the viral NS1 protein, which serves as a ligand for the PDZ domain; 3) the presence of stop codons in the reading frame of PB1-F2 as well as the N66S substitution in the PB1-F2 viral protein; 4) the presence of the polybasic site for hemagglutinin cleavage. The viruses of the H3N1, H3N6, H3N8, H4N6, H1N1, H5N3, and H11N9 subtypes were identified from the group of wild birds’ isolates. All isolates contained the ESEV sequence at the C-terminus of the NS1 protein and the full-length reading frame for the PB1-F2 protein. The replacement of N66S in PB1-F2 was found in six strains. However, the presence of the ESEV sequence (ligand of PDZ domain) in the NS1 virus protein and the N66S substitution in PB1-F2 did not lead to the pathogenicity of these viruses for mice. All isolates demonstrated high yield growth in chicken embryos and were infectious and immunogenic for mice, but did not induce any clinical symptoms.

Published
2017

25. Ability of inactivated vaccines based on far‐eastern tick‐borne encephalitis virus strains to induce humoral immune response in originally seropositive and seronegative recipients

Author

Maikova, Galina B., primary, Chernokhaeva, Liubov L., additional, Rogova, Yulia V., additional, Kozlovskaya, Liubov I., additional, Kholodilov, Ivan S., additional, Romanenko, Victor V., additional, Esyunina, Mariya S., additional, Ankudinova, Anna A., additional, Kilyachina, Anna S., additional, Vorovitch, Mikhail F., additional, and Karganova, Galina G., additional

Published
2018
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27. Recombinant domains III of Tick-Borne Encephalitis Virus envelope protein in combination with dextran and CpGs induce immune response and partial protectiveness against TBE virus infection in mice

Author

Ershova, Anna S., primary, Gra, Olga A., additional, Lyaschuk, Alexander M., additional, Grunina, Tatyana M., additional, Tkachuk, Artem P., additional, Bartov, Mikhail S., additional, Savina, Darya M., additional, Sergienko, Olga V., additional, Galushkina, Zoya M., additional, Gudov, Vladimir P., additional, Kozlovskaya, Liubov I., additional, Kholodilov, Ivan S., additional, Gmyl, Larissa V., additional, Karganova, Galina G., additional, Lunin, Vladimir G., additional, Karyagina, Anna S., additional, and Gintsburg, Alexander L., additional

Published
2016
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28. Ability of inactivated vaccines based on far‐eastern tick‐borne encephalitis virus strains to induce humoral immune response in originally seropositive and seronegative recipients.

Author

Maikova, Galina B., Chernokhaeva, Liubov L., Rogova, Yulia V., Kozlovskaya, Liubov I., Kholodilov, Ivan S., Romanenko, Victor V., Esyunina, Mariya S., Ankudinova, Anna A., Kilyachina, Anna S., Vorovitch, Mikhail F., and Karganova, Galina G.

Abstract

Tick‐borne encephalitis (TBE) remains one of the major public health concerns in northern Eurasia, and its' area is expanding. TBE virus (TBEV) includes three subtypes and several monophyletic groups, cocirculating in Russia. Five inactivated vaccines are used for TBE prophylaxis. The rising number of people subjected to vaccination brings up the issue of the impact of individual recipient characteristics on vaccination efficacy. The present work studies correlations among the vaccination scheme, sex, age, body mass index (BMI), chronic diseases, postvaccinal reaction, pre‐existing anti‐TBEV antibodies, and postvaccinal humoral immunity development. Sera were collected during clinical trials in the TBEV Siberian subtype endemic area. Adult recipients were vaccinated with Tick‐E‐Vac and EnceVir vaccines based on Far‐Eastern TBEV strains. Vaccine ability to induce humoral immunity in different categories of recipients was estimated by seroconversion rates and the percentage of recipients with high neutralizing antibody titers (≥1:500). High immunogenicity of vaccines based on Far‐Eastern TBEV strains in the TBEV Siberian subtype endemic area in all groups of recipients was demonstrated. Impact of pre‐existing contact with the virus and high BMI on humoral immune response development 14 days after the first immunization was evidenced. Nevertheless, the difference was significantly less pronounced 30 days after the first vaccination and undetectable after the second one. 1.Recipients with pre?existing contact with the virus showed higher percent of people with htnAb after the 1st vaccination.2.Recipients with high BMI showed lower percent of people with htnAb after the 1st vaccination.3.All studied individual characteristics did not have impact on the humoral immunity when evaluated after the 2nd vaccination. [ABSTRACT FROM AUTHOR]

Published
2019
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