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Your search keyword '"Khlusevich, Yana"' showing total 17 results
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17 results on '"Khlusevich, Yana"'

1. Novel B-Cell Epitopes of Non-Neutralizing Antibodies in the Receptor-Binding Domain of the SARS-CoV-2 S-Protein with Different Effects on the Severity of COVID-19

Author

Matveev, Andrey L., Pyankov, Oleg V., Khlusevich, Yana A., Tyazhelkova, Olga V., Emelyanova, Ljudmila A., Timofeeva, Anna M., Shipovalov, Andrey V., Chechushkov, Anton V., Zaitseva, Natalia S., Kudrov, Gleb A., Yusubalieva, Gaukhar M., Yussubaliyeva, Saule M., Zhukova, Oxana A., Tikunov, Artem Yu., Baklaushev, Vladimir P., Sedykh, Sergey E., Lifshits, Galina I., and Tikunova, Nina V.

Published
2023
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3. Cross-Reactive Antibodies to the NS1 Protein of Omsk Hemorrhagic Fever Virus Are Absent in the Sera of Patients with Tick-Borne Encephalitis.

Author

Kravchuk, Bogdana I., Khlusevich, Yana A., Chicherina, Galina S., Yakimenko, Valeriy V., Krasnova, Elena I., Tikunova, Nina N., and Matveev, Andrey L.

Subjects
*TICK-borne encephalitis viruses, *TICK-borne encephalitis, *RECOMBINANT proteins, *WESTERN immunoblotting, *VIRAL encephalitis
Abstract

Omsk hemorrhagic fever virus (OHFV) is a member of the tick-borne encephalitis virus (TBEV) complex of the Flaviviridae family. Currently, there are no data on the cross-reactivity of antibodies to the NS1 proteins of OHFV and TBEV. Such data are of major interest for monitoring viral encephalitis of unknown etiology due to the increasing geographical distribution of OHFV. In this study, a recombinant OHFV NS1 protein was produced using the Escherichia coli expression system and purified. The recombinant OHFV NS1 protein was recognized by specific mice immune ascetic fluids to the native OHFV NS1 protein. A Western blot analysis and ELISA of the recombinant NS1 proteins of OHFV and TBEV were used to study the cross-reactivity of antibodies from immune ascites fluid obtained from OHFV-infected mice and mAbs against TBEV NS1. Anti-TBEV NS1 mouse monoclonal antibodies (mAbs) have been shown to not be cross-reactive to the OHFV NS1 protein. Sera from patients with confirmed tick-borne encephalitis (TBE) were examined by ELISA using recombinant OHFV NS1 and TBEV NS1 proteins as antigens. It was shown for the first time that cross-reactive antibodies to the OHFV NS1 protein were not detected in the sera of TBE patients, whereas the sera contained antibodies to the TBEV NS1 protein. [ABSTRACT FROM AUTHOR]

Published
2024
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4. Bacteriophage vB_SepP_134 and Endolysin LysSte_134_1 as Potential Staphylococcus-Biofilm-Removing Biological Agents

Author

Golosova, Natalia N., primary, Matveev, Andrey L., additional, Tikunova, Nina V., additional, Khlusevich, Yana A., additional, Kozlova, Yulia N., additional, Morozova, Vera V., additional, Babkin, Igor V., additional, Ushakova, Tatiana A., additional, Zhirakovskaya, Elena V., additional, Panina, Elizaveta A., additional, Ryabchikova, Elena I., additional, and Tikunov, Artem Y., additional

Published
2024
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5. Bacteriophage St_134 and its Endolysin as a Potential Staphylococcus Biofilm-Removing Biological Agent

Author

Golosova, Natalia N., primary, Matveev, Andrey L., additional, Tikunova, Nina V., additional, Khlusevich, Yana A., additional, Kozlova, Yulia N., additional, Morozova, Vera V., additional, Babkin, Igor V., additional, Ushakova, Tatiana A., additional, Zhirakovskaya, Elena V., additional, Panina, Elisaveta A., additional, Ryabchikova, Elena I., additional, and Tikunov, Artem Y., additional

Published
2024
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7. Bacteriophage St_134 and its Endolysin as a Potential Staphy-lococcus Biofilm-Removing Biological Agent

Author

Golosova, Natalia N., primary, Matveev, Andrey L., additional, Tikunova, Nina V., additional, Khlusevich, Yana A., additional, Kozlova, Yulia N., additional, Morozova, Vera V., additional, Babkin, Igor V., additional, Ushakova, Tatiana A., additional, Zhirakovskaya, Elena V., additional, Panina, Elisaveta A., additional, Ryabchikova, Elena I., additional, and Tikunov, Artem Y., additional

Published
2023
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8. Antibodies Capable of Enhancing SARS-CoV-2 Infection Can Circulate in Patients with Severe COVID-19

Author

Matveev, Andrey, primary, Pyankov, Oleg, additional, Khlusevich, Yana, additional, Tyazhelkova, Olga, additional, Emelyanova, Lyudmila, additional, Timofeeva, Anna, additional, Shipovalov, Andrey, additional, Chechushkov, Anton, additional, Zaitseva, Natalia, additional, Kudrov, Gleb, additional, Yusubalieva, Gaukhar, additional, Yussubaliyeva, Saule, additional, Zhukova, Oxana, additional, Baklaushev, Vladimir, additional, Sedykh, Sergey, additional, Lifshits, Galina, additional, Tikunov, Artem, additional, and Tikunova, Nina, additional

Published
2023
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12. Tick-borne encephalitis nonstructural protein NS1 expressed in E. coli retains immunological properties of the native protein

Author

Matveev, Andrey, Khlusevich, Yana, Golota, Olga, Kravchuk, Bogdana, Tkachev, Sergey, Emelyanova, Lyudmila, and Tikunova, Nina

Subjects
Escherichia coli, Gene Expression, Enzyme-Linked Immunosorbent Assay, Viral Nonstructural Proteins, Antibodies, Viral, Recombinant Proteins, Encephalitis Viruses, Tick-Borne, Biotechnology
Abstract

There is evidence that flaviviral NS1 glycoprotein plays an important role in the pathology of tick-borne encephalitis (TBE) and NS1-specific antibodies are detected in the blood of patients with TBE. This makes NS1 a good target for the development of therapeutic inhibitors and NS1 could be an important biomarker for the early diagnosis of TBE in vaccinated individuals. Eukaryotic expression systems are mainly used to produce recombinant tick-borne encephalitis virus (TBEV) NS1. The expression of TBEV NS1 proteins in eukaryotic cells was successful, but there were some limitations. Several attempts have also been made to obtain the NS1 protein in Escherichia coli cells; however, they were unsuccessful due to the low solubility of the recombinant protein and improper folding. In this study, using Trx-tag as a fusion partner, soluble Trx-fused TBEV NS1 protein was first produced in the E. coli BL21 strain. In addition, insoluble Trx-fused TBEV NS1 protein was obtained when cultivation conditions were changed to increase the productivity. The insoluble TBEV NS1 obtained from inclusion bodies was solubilized using chaotropic reagents and successfully refolded using dialysis. Both soluble variant and successfully refolded from inclusion bodies variant showed immunological properties similar to the native TBEV NS1 protein and were recognized by specific monoclonal antibodies (mAbs), immune ascetic fluid in ELISA, western blot, and competitive analysis.

Published
2022

14. Novel mouse monoclonal antibodies specifically recognizing β-(1→3)-D-glucan antigen

Author

Matveev, Andrey L., primary, Krylov, Vadim B., additional, Khlusevich, Yana A., additional, Baykov, Ivan K., additional, Yashunsky, Dmitry V., additional, Emelyanova, Ljudmila A., additional, Tsvetkov, Yury E., additional, Karelin, Alexander A., additional, Bardashova, Alevtina V., additional, Wong, Sarah S. W., additional, Aimanianda, Vishukumar, additional, Latgé, Jean-Paul, additional, Tikunova, Nina V., additional, and Nifantiev, Nikolay E., additional

Published
2019
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15. Post-exposure administration of chimeric antibody protects mice against European, Siberian, and Far-Eastern subtypes of tick-borne encephalitis virus

Author

Matveev, Andrey L., primary, Kozlova, Irina V., additional, Stronin, Oleg V., additional, Khlusevich, Yana A., additional, Doroshchenko, Elena K., additional, Baykov, Ivan K., additional, Lisak, Oksana V., additional, Emelyanova, Ljudmila A., additional, Suntsova, Olga V., additional, Matveeva, Vera A., additional, Savinova, Julia S., additional, and Tikunova, Nina V., additional

Published
2019
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