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Your search keyword '"Khire, Tejas"' showing total 15 results
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15 results on '"Khire, Tejas"'

2. Rapid Minimum Inhibitory Concentration (MIC) Analysis Using Lyophilized Reagent Beads in a Novel Multiphase, Single-Vessel Assay

Author

Khire, Tejas Suresh, primary, Gao, Wei, additional, Bales, Brian, additional, Hsieh, Kuangwen, additional, Grossmann, Greg, additional, Park, Dong Jin M., additional, O’Keefe, Christine, additional, Brown-Countess, Arnyah, additional, Peterson, Sara, additional, Chen, Fan-En, additional, Lenigk, Ralf, additional, Trick, Alex, additional, Wang, Tza-Huei, additional, and Puleo, Christopher, additional

Published
2023
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7. Brain endothelial tricellular junctions as novel sites for T cell diapedesis across the blood–brain barrier

Author

Castro Dias, Mariana, primary, Odriozola Quesada, Adolfo, additional, Soldati, Sasha, additional, Bösch, Fabio, additional, Gruber, Isabelle, additional, Hildbrand, Tobias, additional, Sönmez, Derya, additional, Khire, Tejas, additional, Witz, Guillaume, additional, McGrath, James L., additional, Piontek, Jörg, additional, Kondoh, Masuo, additional, Deutsch, Urban, additional, Zuber, Benoît, additional, and Engelhardt, Britta, additional

Published
2021
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8. Digital electrical impedance analysis for single bacterium sensing and antimicrobial susceptibility testing

Author

Scherer, Brian, primary, Surrette, Christine, additional, Li, Hui, additional, Torab, Peter, additional, Kvam, Erik, additional, Galligan, Craig, additional, Go, Steven, additional, Grossmann, Greg, additional, Hammond, Tyler, additional, Johnson, Tammy, additional, St-Pierre, Richard, additional, Nelson, John R., additional, Potyrailo, Radislav A., additional, Khire, Tejas, additional, Hsieh, Kuangwen, additional, Wang, Tza-Huei, additional, Wong, Pak Kin, additional, and Puleo, Chris M., additional

Published
2021
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12. A silicon nanomembrane platform for the visualization of immune cell trafficking across the human blood–brain barrier under flow

Author

Mossu, Adrien, primary, Rosito, Maria, additional, Khire, Tejas, additional, Li Chung, Hung, additional, Nishihara, Hideaki, additional, Gruber, Isabelle, additional, Luke, Emma, additional, Dehouck, Lucie, additional, Sallusto, Federica, additional, Gosselet, Fabien, additional, McGrath, James L, additional, and Engelhardt, Britta, additional

Published
2018
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13. Ultrathin Silicon Membranes for in SituOptical Analysis of Nanoparticle Translocation across a Human Blood–Brain Barrier Model

Author

Hudecz, Diána, Khire, Tejas, Chung, Hung Li, Adumeau, Laurent, Glavin, Dale, Luke, Emma, Nielsen, Morten S., Dawson, Kenneth A., McGrath, James L., and Yan, Yan

Abstract

Here we present a blood–brain barrier (BBB) model that enables high-resolution imaging of nanoparticle (NP) interactions with endothelial cells and the capture of rare NP translocation events. The enabling technology is an ultrathin silicon nitride (SiN) membrane (0.5 μm pore size, 20% porosity, 400 nm thickness) integrated into a dual-chamber platform that facilitates imaging at low working distances (∼50 μm). The platform, the μSiM-BBB (microfluidic silicon membrane-BBB), features human brain endothelial cells and primary astrocytes grown on opposite sides of the membrane. The human brain endothelial cells form tight junctions on the ultrathin membranes and exhibit a significantly higher resistance to FITC-dextran diffusion than commercial membranes. The enhanced optical properties of the SiN membrane allow high-resolution live-cell imaging of three types of NPs, namely, 40 nm PS-COOH, 100 nm PS-COOH, and apolipoprotein E-conjugated 100 nm SiO2, interacting with the BBB. Despite the excellent barrier properties of the endothelial layer, we are able to document rare NP translocation events of NPs localized to lysosomal compartments of astrocytes on the “brain side” of the device. Although the translocation is always low, our data suggest that size and targeting ligand are important parameters for NP translocation across the BBB. As a platform that enables the detection of rare transmission across tight BBB layers, the μSiM-BBB is an important tool for the design of nanoparticle-based delivery of drugs to the central nervous system.

Published
2020
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