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We demonstrate improved methods for making valid and accurate comparisons of fluorescence measurement capabilities among instruments tested at different sites and times. We designed a suite of measurements and automated data processing methods to obtain consistent objective results and applied them to a selection of 23 instruments at nine sites to provide a range of instruments as well as multiple instances of similar instruments. As far as we know, this study represents the most accurate methods and results so far demonstrated for this purpose. The first component of the study reporting improved methods for photoelectron scale (Spe) evaluations, which was published previously (Parks, El Khettabi, Chase, Hoffman, Perfetto, Spidlen, Wood, Moore, and Brinkman: Cytometry A 91 (2017) 232-249). Those results which were within themselves are not sufficient for instrument comparisons, so here, we use the Spe scale results for the 23 cytometers and combine them with additional information from the analysis suite to obtain the metrics actually needed for instrument evaluations and comparisons. We adopted what we call the 2+2SD limit of resolution as a maximally informative metric, for evaluating and comparing dye measurement sensitivity among different instruments and measurement channels. Our results demonstrate substantial differences among different classes of instruments in both dye response and detection sensitivity and some surprisingly large differences among similar instruments, even among instruments with nominally identical configurations. On some instruments, we detected defective measurement channels needing service. The system can be applied in shared resource laboratories and other facilities as an aspect of quality assurance, and accurate instrument comparisons can be valuable for selecting instruments for particular purposes and for making informed instrument acquisition decisions. An institutionally supported program could serve the cytometry community by facilitating access to materials, and analysis and maintaining an archive of results. © 2018 International Society for Advancement of Cytometry.

A 3-D X-ray imaging system that eliminates the rotation process associated with tomographic systems is introduced. The system relies on measuring the intensity of Compton scattered radiation in two directions mutually perpendicular to an incident beam that rectilinearly scans the object. These measurements, along with transmission measurements obtained from one-side exposure of the object are utilized to reconstruct 3-D images of three physical parameters: two attenuation coefficients corresponding to the incident and scattered energies, and the electron-density in each voxel. This part of the paper addresses the theoretical and physical aspects associated with the image reconstruction process, and presents examples of images reconstructed from experimental results. [ABSTRACT FROM AUTHOR]

The design aspects of a 3-D X-ray imaging system that eliminates the rotation process associated with tomographic systems are discussed. The processes of selecting the proper X-ray voltage and the beam configuration are addressed. A rectilinear scanning mechanism is introduced. The choice and design of detector assemblies are described. The paper presents the results of experiments devised to progressively test the performance and response of the system. [ABSTRACT FROM AUTHOR]