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Amaç: Bu çalışmanın amacı, Wistar sıçanlarında, kronik bakır sülfat zehirlenmesine (100mg / Kg bw) karşı yaygın alıç Crataegus monogyna sulu ekstraktının (1.5g / Kg bw/ gün) olası faydalı kullanımını araştırmaktır.Gereç ve Yöntem: Hayvanlar, tedavi edilmemiş kontrol (C), sırasıyla alıç meyveleri (F) ve yaprakları (L) ile muamele edilmiş 2 pozitif kontrol, bakır (Cu) ile muamele edilmiş grup ve Cu ve alıç meyveleri (CuF) ve Cu ve alıç yaprakları (CuL) ile muamele edilmiş 2 kombine tedavi grubu olmak üzere 6 gruba ayrılmıştır. 30 günlük oral uygulamadan sonra, epididimal sperm konsantrasyonu, motilite, canlılık, hız (VCL, VSL ve VAP), dış yana baş deplasmanının amplitüdü (ALH) ve sperm kuyruğu vuruş sıklığının (BCF) yanısıra testis ağırlığı ve plazma testosteron seviyeleri değerlendirilmiştir. Ayrıca, testis glutatyonu (GSH), malondialdehid (MDA) ve glutatyonperoksidaz (GPx) da değerlendirilmiştir.Sonuç ve Tartışma: Cu maruziyeti, kontrol gruplarına kıyasla testosteron, sperm konsantrasyonu, canlı sperm, VCL, VSL, VAP, ALH, BCF, GSH ve GPx seviyelerini azaltmıştır. Cu grubunun sıçanlarında ölü sperm ve MDA seviyeleri, tedavi edilmeyen kontrole kıyasla artmıştır. Cu grubuyla karşılaştırıldığında, testosteron seviyeleri, sperm konsantrasyonu, sperm motilitesi, canlı sperm, VCL, VSL, VAP, ALH, BCF, GSH ve GPx seviyeleri CuF ve CuL gruplarında çok daha yüksek ve MDA konsantrasyonları ise anlamlı olarak düşüktür. Sonuç olarak, alıç, Cu ile sulu bir özüt olarak uygulandığında, bakır toksisitesine karşı çoğu biyolojik belirteci korumuş ve sperm konsantrasyonunu ve hızını (VCL ve VAP) artırmıştır, Objective: The purpose of this study was to investigate the possible beneficial use of common hawthorn Crataegus monogyna aqueous extract at 1.5g/Kg bw/day against chronic copper sulfate intoxication (100mg/Kg bw) in Wistar rats.Material and Method: Animals were divided into 6 groups; the untreated control (C), 2 positive controls treated respectively with hawthorn fruits (F) and leaves (L), 1 group treated with copper (Cu) and 2 combined treatment groups treated with Cu and hawthorn-fruits (CuF) and Cu and hawthorn-leaves (CuL). After 30 days of oral administration, testis weight and plasma testosterone levels were evaluated, in addition to the epididymal sperm concentration, motility, vitality, velocity (VCL, VSL and VAP), the amplitude of lateral head displacement (ALH), and the beat cross frequency (BCF). Testicular glutathione (GSH), malondialdehyde (MDA), and glutathione peroxidase (GPx) were also evaluated.Result and Discussion: Cu exposure reduced testosterone, sperm concentration, live sperm, VCL, VSL, VAP, ALH, BCF, GSH, and GPx levels compared to control groups. Dead sperm and MDA levels were increased in rats of Cu group compared to the untreated control. When compared to the Cu group, levels of testosterone, sperm concentration, sperm motility, live sperm, VCL, VSL, VAP, ALH, BCF, GSH, and GPx were much higher in the CuF and CuL groups, along with a significantly lower MDA concentration. In conclusion, hawthorn, when co-administered as an aqueous extract with Cu, protected most biological markers against copper toxicit, while positive control (s) boosted sperm concentration and velocity (VCL and VAP).

La consommation chronique d'éthanol est associée à des troubles de la reproduction chez l’homme. Pour cela, l’objectif de ce travail est d’évaluer chez le lapin mâle adulte Oryctolagus cuniculus l’effet de la consommation chronique d’éthanol sur la reproduction. L’alcool concentré à 20%, 25% et 30% a été administré au lapin mâle par gavage pendant six semaines successives. Après sacrifice des animaux, les testicules et l’épididyme ont été prélevés afin d’évaluer certains paramètres de la reproduction, alors que le sang a été recueilli pour le dosage de la testostérone. Les résultats obtenus montrent une diminution de la mobilité, la vitesse, la vitalité et la concentration des spermatozoïdes, accompagnée d’une augmentation de leur nombre malformés chez les trois lots d’animaux traités par rapport au lot témoin. Par ailleurs, le traitement à l’alcool a entrainé une diminution du poids des testicules et de l’épididyme, ainsi qu’une diminution du taux de la testostérone chez les animaux des groupes traités comparés au groupe témoin. Les résultats montrent clairement que l’éthanol exerce un effet toxique sur la fertilité du lapin mâle.© 2015 International Formulae Group. All rights reserved.Mots clés: Alcool, épididyme, spermatozoïde, testicule, testostéroneEnglish AbstractChronic ethanol consumption is associated with reproductive disorders in humans. Therefore, the objective of this study was to evaluate in adult male rabbit Oryctolagus cuniculus the effect of chronic ethanol consumption on reproduction. The concentrated alcohol 20%, 25% and 30% was administered to male rabbits by gavage for six successive weeks. After sacrificing the animals, testis and epididymis were collected to assess certain parameters of the reproduction, while the blood was collected for the determination of testosterone. The results show a decrease in mobility, speed, vitality and spermatozoa concentration, accompanied by an increase in the number of malformed spermatozoa in three batches of treated animals compared to the control group. Moreover, the treatment with alcohol resulted in a decreased weight of the testes and epididymis, and decreased testosterone levels in animals treated groups compared to the control group. The results clearly show that ethanol exerts a toxic effect on the fertility of male rabbit.© 2015 International Formulae Group. All rights reserved.Keywords: Alcohol, epididymis, spermatozoa, testis, testosterone