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Background: Menstrual hygiene management is a significant issue affecting the health of women around the world. Lack of knowledge and awareness about menstruation and the silence surrounding it is a risk factor for all women. Objective: The objectives of this study were to elicit the knowledge, attitudes, and practices of adolescent girls (13-15 years) regarding issues related to menstruation and to assess the barriers that prevent them from practising menstrual hygiene. Design and Setting: Cross-sectional study involving 187 adolescent girls from four government schools of Delhi. Method: Information was obtained using a mixed-methods approach, including a pretested questionnaire and focus group discussions with girls, parents and school staff. Results: Only 40% of girls were aware of menstruation prior to menarche. The majority of girls (95.7%) did not know the source of menstrual blood. About two-thirds (65.8%) of girls were unaware that the menstrual blood flow could be affected by anaemia. Approximately 17% of the girls believed a woman to be impure during menstruation. About half of the girls absented themselves from school for the first 2-3 days of their menstrual cycle. Thirty-four percent girls did not bathe at all during their menstrual period and a similar number of girls did not bathe daily while menstruating. Dysmenorrhoea was a common problem among 60% of girls. The mothers of girls were identified as the major source of information about menstruation. However, mothers believed that school teachers should also be imparting this knowledge. Teachers expressed the belief that menstrual hygiene was poor among girls. The provision of free sanitary napkins in school has improved hygiene levels. Conclusion: Strategic behaviour change interventions should be designed involving all stakeholders to ensure a sustained change in knowledge, attitude and practices so as to ensure the better health of adolescent girls.

Retinoic acid (RA) is essential for gut endoderm development and has been extensively used for in vitro pancreatic differentiation from human pluripotent stem cells. However, the gene regulatory network triggered by RA signaling remains poorly addressed. Also, whether RA signals control histone modifiers such as the Polycomb group proteins during pancreatic specification remains to be explored. Here, we assess the role of RA on pancreas-specific genes during the differentiation of human embryonic stem cells (hESCs). We demonstrate that RA helps cells exit the definitive endoderm stage and proceed toward a pancreatic fate. Inhibition of the RA pathway using the pharmacological inhibitor LE135 impairs the induction of pancreatic endoderm (PE) markers FOXA2, HNF4α, HNF1β, HHEX, and PDX1. We further determine that RA signals alter the expression of epigenetic-associated genes BMI1 and RING1B in the hESC-derived pancreatic progenitors. These findings broaden our understanding of the mechanisms that drive early PE specification. [ABSTRACT FROM AUTHOR]

Background: Inappropriate menstrual care practices result in adverse health consequences among girls. Developing and implementing interventions that minimize these adverse consequences and facilitate development of healthy menstrual behavior are a priority for any nation.Objectives: This study aimed at collating, summarizing and reviewing evidence to assess the effectiveness of interventions to improve menstrual health and the challenges faced in doing the same.Methods: A systematic review of studies published in peer-reviewed journals and project reports was conducted. Intervention studies related to menstrual health management conducted from 2007 to 2018 were analyzed. A total of 27 interventions conducted among young girls in different countries were identified and study characteristics and outcomes were reviewed.Results: Different intervention studies used a variety of methods like lectures, discussions, demonstrations using multiple audio-visual aids and provision of resources like menstrual cups, sanitary pads and washing soap to spread awareness about menstrual hygiene. Most of the interventions reported a positive impact on the awareness and menstrual practices of girls. However, in a few studies no significant change was observed in the attitude regarding regular bathing, practices related to self-medication for dysmenorrhea and socio-cultural taboos.Conclusion: This review of literature has offered insights into the scope and development of future interventions so that apart from increasing awareness and knowledge on menstrual health issues, sustained behavior change can be brought about among girls to improve their health. [ABSTRACT FROM AUTHOR]

Aim MicroRNAs (miRNAs) constitute a class of small non-coding RNAs involved in regulation of cognate mRNAs post-transcriptionally. MicroRNAs have been implicated in regulating the stem cell differentiation process. Limited regulatory miRNAs have been reported to date during hepatic differentiation of stem cells. The present study was designed to identify the signature miRNAs implicated in hepatic differentiation of stem cells using next-generation sequencing methods. Methods We undertook sequencing of miRNAs isolated from three different time points during hepatic differentiation of human umbilical cord Wharton's jelly-derived mesenchymal stem cells (hUC-MSCs) from two biological replicates. Results Out of a total known 2588 miRNAs (according to miRBase version 21), 880 miRNAs were identified in our study. A total of 63 significantly expressed miRNAs during hepatic differentiation, with at least 2-fold change and a false discovery rate value <0.05, were considered for further analysis. The putative target genes of significantly downregulated miRNAs during hepatic differentiation appeared to be mostly associated with biological processes that are essential for hepatic differentiation and maintenance of mature hepatic phenotype-like liver development, stem cell differentiation, Wnt receptor signaling pathway, and drug and cholesterol metabolic processes. Putative target genes of significantly upregulated miRNAs are highly enriched in regulating processes that block hepatic differentiation of hUC-MSCs like epithelial-mesenchymal transition, transforming growth factor-β receptor signaling pathway, and stem cell maintenance. Conclusion The study provides a new insight for investigation of miRNA-regulated pathways during the differentiation process. [ABSTRACT FROM AUTHOR]