14 results on '"Khanicheh E"'
Search Results
2. 180Noninvasive ultrasound molecular imaging of the effect of atorvastatin on vascular inflammation
- Author
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Khanicheh, E, Mitterhuber, M, Xu, L, Haeuselmann, S, Kuster, G, Lindner, JR, and Kaufmann, BA
- Published
- 2011
3. Dual targeting improves capture of ultrasound microbubbles towards activated platelets but yields no additional benefit for imaging of arterial thrombosis
- Author
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Günther, F., primary, Heidt, T., additional, Kramer, M., additional, Khanicheh, E., additional, Klibanov, A. L., additional, Geibel-Zehender, A., additional, Ferrante, E. A., additional, Hilgendorf, I., additional, Wolf, D., additional, Zirlik, A., additional, Reinöhl, J., additional, Bode, C., additional, Peter, K., additional, Kaufmann, B. A., additional, and Mühlen, C. von zur, additional
- Published
- 2017
- Full Text
- View/download PDF
4. Young Investigator Award session - Basic Science: Thursday 4 December 2014, 10:00-11:00 * Location: Agora
- Author
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Schmutzler, D., primary, Khanicheh, E., additional, Xu, L., additional, Mitterhuber, M., additional, Glatz, K., additional, Ellertsdottir, E., additional, Kaufmann, B., additional, Bala, G., additional, Blykers, A., additional, Xavier, C., additional, Gillis, K., additional, Tierens, S., additional, Descamps, B., additional, Vanhove, C., additional, Lahoutte, T., additional, Cosyns, B., additional, Hernot, S., additional, Ferferieva, V., additional, Deluyker, D., additional, Arslan, T., additional, Lambrichts, I., additional, Rigo, J., additional, Bito, V., additional, Sanz, M., additional, Sitges, M., additional, Bijnens, B., additional, Rubies, C., additional, Batlle, M., additional, Mont, L., additional, Brugada, J., additional, and Guasch, E., additional
- Published
- 2014
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5. Von Willebrand factor binds to surface-bound C1q in vitro
- Author
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Kölm, R., primary, Schaller, M., additional, Vanhecke, D., additional, Hovinga, J.A. Kremer, additional, Khanicheh, E., additional, Kaufmann, B.A., additional, and Trendelenburg, M., additional
- Published
- 2013
- Full Text
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6. Oral Abstract Sessions: Young Investigator Award session * Thursday 8 December 2011, 12:45-13:45 * Location: Pecs
- Author
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Khanicheh, E., primary, Mitterhuber, M., additional, Xu, L., additional, Haeuselmann, S., additional, Kuster, G., additional, Lindner, J., additional, Kaufmann, B., additional, Couade, M., additional, Pernot, M., additional, Mateo, P., additional, Crozatier, B., additional, Fischmeister, R., additional, Tanter, M., additional, Hjertaas, J., additional, Fossa, H., additional, Dybdahl, G., additional, Gruner, R., additional, Lunde, P., additional, and Matre, K., additional
- Published
- 2011
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7. Young Investigator Award session - Basic Science: Thursday 4 December 2014, 10:00-11:00 * Location: Agora
- Author
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Schmutzler, DC, Khanicheh, E, Xu, L, Mitterhuber, M, Glatz, K, Ellertsdottir, E, Kaufmann, BA, Bala, G, Blykers, A, Xavier, C, Gillis, K, Tierens, S, Descamps, B, Vanhove, C, Lahoutte, T, Cosyns, B, Hernot, S, Ferferieva, V, Deluyker, D, Arslan, T, Lambrichts, I, Rigo, JM, Bito, V, Sanz, M, Sitges, M, Bijnens, B, Rubies, C, Batlle, M, Mont, LL, Brugada, J, and Guasch, E
- Abstract
Purpose: Cardiac tests for diagnosing myocarditis lack sensitivity or specificity. We hypothesized that contrast enhanced ultrasound molecular imaging (CEUMI) could detect endothelial inflammation and the recruitment of specific cellular components of the inflammatory response in murine myocarditis. Methods: Microbubbles (MB) bearing antibodies targeting lymphocyte CD4 (MBCD4), endothelial P-Selectin (MBPSel), MB with a control antibody (MBCtr) and MB with a negative electrical charge for targeting of leukocytes (MBN) were prepared. Attachment of MBCD4 was validated in vitro with murine spleen CD4+ lymphocytes. 20 mice were studied after induction of autoimmune myocarditis by immunisation with α-myosin-peptide, 20 mice served as controls. CEUMI of the heart was performed with MBCD4, MBPSel, MBN, and MBCtr. Left ventricular ejection fraction (LVEF) and circumferential strain (CS) were measured. A pathologist blinded to all other data graded the severity of myocarditis on a scale from 0 (no myocarditis) to 4 on histology. Animals were grouped into NM (no myocarditis), MM (moderate myocarditis, score 1-2) and SM (severe myocarditis, score 3-4). Results: In vitro, attachment of MBCD4 to CD4+ lymphocytes was significantly greater than MBCtr (p<0.01). LVEF did not differ between groups (NM 71 ± 13%, MM 73 ± 7%, SM 62 ± 20%, p=0.5), nor did CS (NM 36 ± 9%, MM 25 ± 11%, SM 30 ± 7%, p=0.4). CEUMI (figure) showed increased signal for targeted MB vs MBCtr in MM and SM, whereas signals in NM did not differ from MBCtr. Conclusions: CEUMI can detect endothelial inflammation and leukocyte infiltration in myocarditis, while functional imaging fails to do so. In particular, imaging of CD4+ lymphocytes involved in autoimmune responses in myocarditis is possible. CEUMI may be a powerful method for assessing myocarditis.
Figure CEUMI in Myocarditis (mean ± SEM) - Published
- 2014
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8. Von Willebrand Factor Interacts with Surface-Bound C1q and Induces Platelet Rolling.
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Kölm R, Schaller M, Roumenina LT, Niemiec I, Kremer Hovinga JA, Khanicheh E, Kaufmann BA, Hopfer H, and Trendelenburg M
- Subjects
- Antigen-Antibody Complex metabolism, Apoptosis, Autoantibodies immunology, Autoantibodies metabolism, Cell Membrane metabolism, Cells, Cultured, Collagen Type I metabolism, Hemostasis, Humans, Kidney pathology, Lupus Erythematosus, Systemic immunology, Platelet Activation, Protein Binding, Atherosclerosis immunology, Blood Platelets immunology, Complement C1q metabolism, Kidney metabolism, Lupus Erythematosus, Systemic complications, Thrombosis immunology, von Willebrand Factor metabolism
- Abstract
Premature atherosclerosis and thrombotic complications are major causes of morbidity and mortality in patients with systemic lupus erythematosus (SLE). However, the high incidence of these complications cannot be explained by traditional risk factors alone, suggesting direct effects of an activated immune system on hemostasis. The unexpected nucleotide sequence homology between SLE patient-derived autoantibodies against complement C1q (Fab anti-C1q) and von Willebrand factor (VWF) led us to investigate a potential interaction between the complement and hemostatic systems on the level of initiating molecules. VWF was found to bind to surface-bound C1q under static conditions. The binding could specifically be inhibited by Fab anti-C1q and C1q-derived peptides. Under shear stress the C1q-VWF interaction was enhanced, resembling the binding of VWF to collagen I. Additionally, we could show that C1q-VWF complexes induced platelet rolling and firm adhesion. Furthermore, we observed VWF binding to C1q-positive apoptotic microparticles and cholesterol crystals, as well as increased VWF deposition in C1q-positive glomeruli of SLE patients compared with control nephropathy. We show, to our knowledge for the first time, binding of VWF to C1q and thus a direct interaction between starter molecules of hemostasis and the classical pathway of complement. This direct interaction might contribute to the pathogenic mechanisms in complement-mediated, inflammatory diseases., (Copyright © 2016 by The American Association of Immunologists, Inc.)
- Published
- 2016
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9. Noninvasive Contrast-Enhanced Ultrasound Molecular Imaging Detects Myocardial Inflammatory Response in Autoimmune Myocarditis.
- Author
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Steinl DC, Xu L, Khanicheh E, Ellertsdottir E, Ochoa-Espinosa A, Mitterhuber M, Glatz K, Kuster GM, and Kaufmann BA
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- Animals, Autoimmune Diseases chemically induced, Autoimmune Diseases metabolism, Autoimmune Diseases pathology, Biomarkers metabolism, CD4-Positive T-Lymphocytes immunology, Cells, Cultured, Disease Models, Animal, Female, Inflammation Mediators immunology, Mice, Inbred BALB C, Microbubbles, Myocardial Contraction, Myocarditis chemically induced, Myocarditis metabolism, Myocarditis pathology, Myocardium immunology, Myocardium pathology, P-Selectin metabolism, Peptide Fragments, Predictive Value of Tests, Severity of Illness Index, Stroke Volume, Ventricular Function, Left, Ventricular Myosins, Autoimmune Diseases diagnostic imaging, CD4-Positive T-Lymphocytes metabolism, CD4-Positive T-Lymphocytes pathology, Contrast Media administration & dosage, Echocardiography, Doppler, Pulsed, Inflammation Mediators metabolism, Molecular Imaging methods, Myocarditis diagnostic imaging, Myocardium metabolism
- Abstract
Background: Cardiac tests for diagnosing myocarditis lack sensitivity or specificity. We hypothesized that contrast-enhanced ultrasound molecular imaging could detect myocardial inflammation and the recruitment of specific cellular subsets of the inflammatory response in murine myocarditis., Methods and Results: Microbubbles (MB) bearing antibodies targeting lymphocyte CD4 (MBCD4), endothelial P-selectin (MBPSel), or isotype control antibody (MBIso) and MB with a negative electric charge for targeting of leukocytes (MBLc) were prepared. Attachment of MBCD4 was validated in vitro using murine spleen CD4+ T cells. Twenty-eight mice were studied after the induction of autoimmune myocarditis by immunization with α-myosin-peptide; 20 mice served as controls. Contrast-enhanced ultrasound molecular imaging of the heart was performed. Left ventricular function was assessed by conventional and deformation echocardiography, and myocarditis severity graded on histology. Animals were grouped into no myocarditis, moderate myocarditis, and severe myocarditis. In vitro, attachment of MBCD4 to CD4+ T cells was significantly greater than of MBIso. Of the left ventricular ejection fraction or strain and strain rate readouts, only longitudinal strain was significantly different from control animals in severe myocarditis. In contrast, contrast-enhanced ultrasound molecular imaging showed increased signals for all targeted MB versus MBIso both in moderate and severe myocarditis, and MBCD4 signal correlated with CD4+ T-lymphocyte infiltration in the myocardium., Conclusions: Contrast-enhanced ultrasound molecular imaging can detect endothelial inflammation and leukocyte infiltration in myocarditis in the absence of a detectable decline in left ventricular performance by functional imaging. In particular, imaging of CD4+ T cells involved in autoimmune responses could be helpful in diagnosing myocarditis., (© 2016 American Heart Association, Inc.)
- Published
- 2016
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10. Contrast agents and ionization with respect to safety for patients and doctors.
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von Tengg-Kobligk H, Kara L, Klink T, Khanicheh E, Heverhagen JT, and Böhm IB
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- Acute Kidney Injury etiology, Adrenal Cortex Hormones therapeutic use, Histamine H1 Antagonists, Non-Sedating therapeutic use, Humans, Hypersensitivity etiology, Hypersensitivity prevention & control, Iodine adverse effects, Occupational Injuries etiology, Occupational Injuries prevention & control, Radiation Injuries etiology, Radiation Protection, Radiography, Renal Dialysis, Renal Insufficiency, Chronic therapy, Acute Kidney Injury prevention & control, Contrast Media adverse effects, Occupational Health, Patient Safety, Physicians, Radiation Injuries prevention & control, Radiation, Ionizing, Renal Insufficiency, Chronic diagnostic imaging
- Abstract
In hemodialysis patients, radiographic imaging with iodinated contrast medium (ICM) application plays a central role in the diagnosis and/or follow-up of disease-related conditions. Therefore, safety aspects concerning ICM administration and radiation exposure have a great impact on this group of patients. Current hardware and software improvements including the design and synthesis of modern contrast compounds allow the use of very small amounts of ICM in concert with low radiation exposure. Undesirable ICM side effects are divided into type A (predictable reactions such as heat feeling, headache, and contrast-induced acute kidney injury, for example) and type B (nonpredictable or hypersensitivity) reactions; this chapter deals with the latter. The first onset cannot be prevented. To prevent hypersensitivity upon reexposure of ICM, an allergological workup is recommended. If this is not possible and ICM is necessary, the patient should receive a premedication (H1 antihistamine with or without corticosteroids). Current imaging hardware and software improvements (e.g. such as additional filtration of the X-ray beam) allow the use of very small amount of ICM and small X-ray doses. Proper communication among the team involved in the treatment of a patient may allow to apply imaging protocols and efficient imaging strategies limiting radiation exposure to a minimum. Practical recommendations will guide the reader how to use radiation and ICM efficiently to improve both patient and staff safety., (© 2015 S. Karger AG, Basel.)
- Published
- 2015
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11. Molecular imaging reveals rapid reduction of endothelial activation in early atherosclerosis with apocynin independent of antioxidative properties.
- Author
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Khanicheh E, Qi Y, Xie A, Mitterhuber M, Xu L, Mochizuki M, Daali Y, Jaquet V, Krause KH, Ruggeri ZM, Kuster GM, Lindner JR, and Kaufmann BA
- Subjects
- APOBEC-1 Deaminase, Animals, Antioxidants pharmacology, Aortic Diseases diagnostic imaging, Aortic Diseases genetics, Aortic Diseases metabolism, Aortic Diseases pathology, Atherosclerosis diagnostic imaging, Atherosclerosis genetics, Atherosclerosis metabolism, Atherosclerosis pathology, Biomarkers metabolism, Blotting, Western, Contrast Media, Cytidine Deaminase deficiency, Cytidine Deaminase genetics, Disease Models, Animal, Endothelium, Vascular diagnostic imaging, Endothelium, Vascular metabolism, Endothelium, Vascular pathology, Enzyme Inhibitors pharmacology, Macrophages drug effects, Macrophages metabolism, Male, Mice, Mice, Inbred C57BL, Mice, Knockout, Microbubbles, NADPH Oxidases antagonists & inhibitors, NADPH Oxidases metabolism, Oxidative Stress drug effects, Phenotype, Platelet Adhesiveness drug effects, Platelet Glycoprotein GPIb-IX Complex metabolism, Receptors, LDL genetics, Receptors, LDL metabolism, Superoxides metabolism, Time Factors, Vascular Cell Adhesion Molecule-1 metabolism, Acetophenones pharmacology, Anti-Inflammatory Agents pharmacology, Aortic Diseases drug therapy, Atherosclerosis drug therapy, Endothelium, Vascular drug effects, Molecular Imaging methods, Ultrasonography, Interventional
- Abstract
Objective: Antioxidative drugs continue to be developed for the treatment of atherosclerosis. Apocynin is an nicotinamide adenine dinucleotide phosphate oxidase inhibitor with anti-inflammatory properties. We used contrast-enhanced ultrasound molecular imaging to assess whether short-term apocynin therapy in atherosclerosis reduces vascular oxidative stress and endothelial activation, Approach and Results: Genetically modified mice with early atherosclerosis were studied at baseline and after 7 days of therapy with apocynin (4 mg/kg per day IP) or saline. Contrast-enhanced ultrasound molecular imaging of the aorta was performed with microbubbles targeted to vascular cell adhesion molecule 1 (VCAM-1; MB(V)), to platelet glycoprotein Ibα (MB(Pl)), and control microbubbles (MB(Ctr)). Aortic vascular cell adhesion molecule 1 was measured using Western blot. Aortic reactive oxygen species generation was measured using a lucigenin assay. Hydroethidine oxidation was used to assess aortic superoxide generation. Baseline signal for MBV (1.3 ± 0.3 AU) and MB(Pl )(1.5 ± 0.5 AU) was higher than for MBCtr (0.5 ± 0.2 AU; P<0.01). In saline-treated animals, signal did not significantly change for any microbubble agent, whereas short-term apocynin significantly (P<0.05) reduced vascular cell adhesion molecule 1 and platelet signal (MBV: 0.3 ± 0.1; MBPl: 0.4 ± 0.1; MBCtr: 0.3 ± 0.2 AU; P=0.6 between agents). Apocynin reduced aortic vascular cell adhesion molecule 1 expression by 50% (P<0.05). However, apocynin therapy did not reduce reactive oxygen species content, superoxide generation, or macrophage content., Conclusions: Short-term treatment with apocynin in atherosclerosis reduces endothelial cell adhesion molecule expression. This change in endothelial phenotype can be detected by molecular imaging before any measurable decrease in macrophage content and is not associated with a detectable change in oxidative burden.
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- 2013
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12. Noninvasive ultrasound molecular imaging of the effect of statins on endothelial inflammatory phenotype in early atherosclerosis.
- Author
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Khanicheh E, Mitterhuber M, Xu L, Haeuselmann SP, Kuster GM, and Kaufmann BA
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- Animals, Aorta diagnostic imaging, Aorta metabolism, Aorta pathology, Atherosclerosis metabolism, Cholesterol blood, Disease Models, Animal, Endothelium, Vascular metabolism, Inflammation metabolism, Inflammation pathology, Mice, Plaque, Atherosclerotic, Ultrasonography, Vascular Cell Adhesion Molecule-1 metabolism, Atherosclerosis diagnostic imaging, Endothelium, Vascular drug effects, Endothelium, Vascular pathology, Hydroxymethylglutaryl-CoA Reductase Inhibitors pharmacology, Inflammation diagnostic imaging, Molecular Imaging, Phenotype
- Abstract
Background/objectives: Inflammatory changes on the endothelium are responsible for leukocyte recruitment to plaques in atherosclerosis. Noninvasive assessment of treatment-effects on endothelial inflammation may be of use for managing medical therapy and developing novel therapies. We hypothesized that molecular imaging of vascular cell adhesion molecule-1 (VCAM-1) with contrast enhanced ultrasound (CEU) could assess treatment effects on endothelial phenotype in early atherosclerosis., Methods: Mice with atherosclerosis produced by gene deletion of the LDL-receptor and Apobec-1-editing protein were studied. At 12 weeks of age, mice received 8 weeks of regular chow or atorvastatin-enriched chow (10 mg/kg/day). At 20 weeks, CEU molecular imaging for aortic endothelial VCAM-1 expression was performed with VCAM-1-targeted (MB(VCAM)) and control microbubbles (MB(Ctr)). Aortic wall thickness was assessed with high frequency ultrasound. Histology, immunohistology and Western blot were used to assess plaque burden and VCAM-1 expression., Results: Plaque burden was reduced on histology, and VCAM-1 was reduced on Western blot by atorvastatin, which corresponded to less endothelial expression of VCAM-1 on immunohistology. High frequency ultrasound did not detect differences in aortic wall thickness between groups. In contrast, CEU molecular imaging demonstrated selective signal enhancement for MB(VCAM) in non-treated animals (MB(VCAM) 2±0.3 vs MB(Ctr) 0.7±0.2, p<0.01), but not in statin-treated animals (MB(VCAM) 0.8±0.2 vs MB(Ctr) 1.0±0.2, p = ns; p<0.01 for the effect of statin on MB(VCAM) signal)., Conclusions: Non-invasive CEU molecular imaging detects the effects of anti-inflammatory treatment on endothelial inflammation in early atherosclerosis. This easily accessible, low-cost technique may be useful in assessing treatment effects in preclinical research and in patients.
- Published
- 2013
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13. Factors affecting the endothelial retention of targeted microbubbles: influence of microbubble shell design and cell surface projection of the endothelial target molecule.
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Khanicheh E, Mitterhuber M, Kinslechner K, Xu L, Lindner JR, and Kaufmann BA
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- Animals, Antibodies, Monoclonal metabolism, Cell Adhesion, Equipment Design, Glycocalyx, Image Enhancement, Male, Mice, Mice, Inbred C57BL, Models, Animal, Ultrasonography methods, Antigens, CD metabolism, Cell Adhesion Molecules metabolism, Contrast Media metabolism, Endothelium, Vascular diagnostic imaging, Endothelium, Vascular metabolism, Microbubbles, P-Selectin metabolism
- Abstract
Background: In biologic systems, the arrest of circulating cells is mediated by adhesion molecules projecting their active binding domain above the cell surface to enhance bond formation and tether strength. Similarly, molecular spacers are used for ligands on particle-based molecular imaging agents. The aim of this study was to evaluate the influence of tether length for targeting ligands on ultrasound molecular imaging agents., Methods: Microbubbles bearing biotin at the end of variable-length polyethylene glycol spacer arms (MB(2000) and MB(3400)) were prepared. To assess in vivo attachment efficiency to endothelial counterligands that vary in their distance from the endothelial cell surface, contrast-enhanced ultrasound (CEU) molecular imaging of tumor necrosis factor-α-induced P-selectin (long distance) or intercellular adhesion molecule-2 (short distance) was performed with each agent in murine hind limbs. To assess the influence of the glycocalyx on microbubble attachment, CEU molecular imaging of intercellular adhesion molecule-2 was performed after degradation of the glycocalyx., Results: CEU molecular imaging targeted to P-selectin showed signal enhancement above control agent for MB(2000) and MB(3400), the degree of which was significantly higher for MB(3400) compared with MB(2000). CEU molecular imaging targeted to intercellular adhesion molecule-2 showed low overall signal for all agents and signal enhancement above control for MB(3400) only. Glycocalyx degradation increased signal for MB(3400) and MB(2000)., Conclusions: Microbubble targeting to endothelial ligands is influenced by (1) the tether length of the ligand, (2) the degree to which the endothelial target is projected from the cell surface, and (3) the status of the glycocalyx. These considerations are important for designing targeted imaging probes and understanding potential obstacles to molecular imaging., (Copyright © 2012 American Society of Echocardiography. Published by Mosby, Inc. All rights reserved.)
- Published
- 2012
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14. Congenital plexiform schwannoma of the clitoris.
- Author
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Yegane RA, Alaee MS, and Khanicheh E
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- Child, Female, Humans, Neurilemmoma pathology, Vulvar Neoplasms pathology, Clitoris, Neurilemmoma congenital, Vulvar Neoplasms congenital
- Abstract
Schwannomas are slow-growing nerve sheet neoplasms which are rarely found in the female genital system. In this article, we present a patient with Antony A congenital plexiform schwannoma of the clitoris. A 6-year-old girl was brought to our hospital with the history of a firm non-cystic clitoral mass from birth, which had been growing more rapidly during the previous year. The patient was scheduled for surgery. Histological studies revealed plexiform schwannoma of the clitoris post-operatively. Therefore, schwannoma should be considered in the differential diagnosis of clitoral masses.
- Published
- 2008
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