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Root-knot nematodes (Meloidogynespp.) are sedentary endoparasites and soil-borne pathogens worldwide. M. incognitais one of the most devastating and dominant species among them causing economic yield losses in almost all vegetables and other agricultural crops in the world. Current management strategies against M. incognitaare not sufficient. However, from the last decades, utilization of nematicides has been increasing to manage this pest due to which environmental and human health issues arises. Bio-organic approaches are the best alternatives to nematicides, including biological agents and organic matters. In bio agents both arbuscular mycorrhizal and nematophagous fungi have a potent ability to manage plant-parasitic nematodes by inducing systemic resistance and activation of pathogenesis related (PR) genes in inoculated plants against nematodes, whereas nematophagous fungi trap nematodes for their feeding and killing them. Soil application of organic matters viz., botanical extract, oil cakes and agricultural wastes both in vitroand in vivois also useful. Botanical extracts, oil cakes, kill nematodes by releasing secondary metabolites and inhibiting the movement of juveniles in the soil. Researchers from all over the world engage in evolving eco-friendly approaches that enhance and sustain the vegetable and agricultural production against this pest and keep it below the threshold level without affecting beneficial soil microbiota. In the future, such environment benign approaches have become an active field of research that adds new knowledge for their success against pest management, and enhancement of agricultural production for the human population.

Twelve bacterial strains (GJ01 to GJ12), isolated from an oil-contaminated soil, were characterized through different biochemical, morphological, and molecular technique and analyzed by different screening methods to choose the best biosurfactant producing strain. Only GJ-01 strain exhibited significant biosurfactant activity, while the other strains exhibited partial activity. Molecular identification through 16S  rRNA technique identified GJ-01 as a Bacillus siamensis-AMU03. The effect of the cultural filtrate tested under in vitro study against Rhizoctonia solaniand Fusarium oxysporum, with the highest reduction of mycelial growth in dual culture method (89.40% and 90.20% respectively). Both in food poisoning method as well as pathogenicity test, the bioagent was able to inhibit the growth of both the pathogens successfully at 3% concentration, and that was mainly due to volatile metabolites such as surfactin, fengycin and iturin, which were identified and analysed through Liquid chromatography-mass spectrometry. These findings indicated that AMU03strain had a promising eco-friendly and bio-safe approach to cope against soil-borne and post-harvest pathogens of seed tubers.