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Abstract The hyoid bone has been identified as sexually dimorphic in various populations. The current study is a forerunner analysis that used three-dimensional multidetector computed tomography (3D MDCT) images of the hyoid bone to examine sexual dimorphism in the Egyptian population. A total of 300 subjects underwent neck CT imaging, with an additional 60 subjects randomly selected for model validation. Ten hyoid variables were measured. Initially, the dataset was subjected to discriminant analysis to predict sex and the critical variables associated with sexual dimorphism. Subsequently, machine learning approaches were employed to enhance the accuracy of sex determination. The results indicated that all measured dimensions of the hyoid bone were substantially larger in males confront to females. Discriminant functions combining four measurements (major and minor axes of the hyoid body, the distance between the lesser horns, and hyoid bone length) achieved a higher accuracy of sex prediction compared to univariate functions. The accuracies of machine learning models ranged from 0.8667 to 0.933 with precision, recall, and F1-scores also showing improvements. These findings underscore the robustness and reliability of hyoid bone in sex discrimination among Egyptians, supported by both traditional statistical methods and machine learning approaches, and could prove invaluable in forensic cases.

Although the combination of antibiotics is generally well-tolerated, they may have nephrotoxic effects. This study investigated whether tigecycline (TG) and gentamicin (GM) co-administration could accelerate renal damage. Male Wistar rats were randomly divided into six experimental groups: the control, TG7 (tigecycline, 7 mg/kg), TG14 (tigecycline, 14 mg/kg), GM (gentamicin, 80 mg/kg), TG7+GM, and TG14+GM groups. The combination of TG and GM evoked renal damage seen by the disruption of kidney function tests. The perturbation of renal tissue was mainly confounded to the TG and GM-induced oxidative damage, which was exhibited by marked increases in renal MDA (malondialdehyde) along with a drastic reduction in GSH (reduced-glutathione) content and CAT (catalase) activity compared to their individual treatments. More obvious apoptotic events and inflammation were also revealed by elevating the annexin-V and interleukin-6 (IL-6) levels, aside from the upregulation of renal PCNA (proliferating cell nuclear antigen) expression in the TG and GM concurrent treatment. The principal component analysis indicated that creatinine, urea, annexin-V, IL-6, and MDA all played a role in discriminating the TG and GM combined toxicity. Oxidative stress, inflammatory response, and apoptosis were the key mechanisms involved in this potentiated toxicity.

CPF (chlorpyrifos) is an organophosphate pesticide used in agricultural and veterinary applications. Our experiment aimed to explore the effects of thymoquinone (TQ) and/or lycopene (LP) against CPF-induced neurotoxicity. Wistar rats were categorized into seven groups: first group served as a control (corn oil only); second group, TQ (10 mg/kg); third group, LP (10 mg/kg); fourth group, CPF (10 mg/kg) and deemed as CPF toxic control; fifth group, TQ + CPF; sixth group, (LP + CPF); and seventh group, (TQ + LP + CPF). CPF intoxication inhibited acetylcholinesterase (AchE), decreased glutathione (GSH) content, and increased levels of malondialdehyde (MDA), an oxidative stress biomarker. Furthermore, CPF impaired the activity of antioxidant enzymes including superoxide dismutase (SOD) and catalase (CAT) along with enhancement of the level of inflammatory mediators such as tumor necrosis factor-α (TNF-α), interleukin (IL)-6, and IL-1β. CPF evoked apoptosis in brain tissue. TQ or LP treatment of CPF-intoxicated rats greatly improved AchE activity, oxidative state, inflammatory responses, and cell death. Co-administration of TQ and LP showed better restoration than their sole treatment. In conclusion, TQ or LP supplementation may alleviate CPF-induced neuronal injury, most likely due to TQ or LPs’ antioxidant, anti-inflammatory, and anti-apoptotic effects.

Background: Sex determination of the human skeleton has been studied in forensic and physical anthropology. Long bones have been especially used because of the ease of defining measurements and better preservation. The femur is the most studied of all long bones. The application of PCR to forensic medicine has greatly enhanced the power and sensitivity of DNA analysis which has become a valuable tool for the identification of missing individuals and unknown remains. Aim of work: Determination of sex from the femur bones and comparing the anatomical method with that of SRY gene detection. Materials and methods: A total of 20 adult Egyptian human bones were selected for this study. They were obtained from the Unit of Anthropology, Anatomy Department, Faculty of Medicine, Cairo University. The diameter of the femoral head and the femoral mid-shaft circumference were used as metric measurements in sex determination of the femur. A multiplex method for DNA sex determination using the sex-determining region Y (SRY) gene was used. It involved two sets of primers, amplifying two different DNA sequences in a single reaction. Results: By measuring both the femoral head diameter and the mid-shaft circumference, it was revealed that 8 out of the 20 specimens were males while the rest were females. On the other hand, using the DNA analysis, all the samples showed amplification of one fragment (239/242 bp) in case of female origin, and two fragments (239/242 bp and 139 bp) in case of male origin. This DNA analysis verified the results obtained using metric measurements of the femur. Conclusion: The SRY gene detection method for sex determination is quick and simple, requiring only one PCR reaction. It corroborates the results obtained from anatomical measurements and further confirms the sex of the femur bone in question.