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Numerous studies have demonstrated that dysfunctional high-density lipoprotein (HDL), especially oxidized HDL (OxHDL), could generate multifaceted in vivo proatherogenic effects that run counter to the antiatherogenic activities of HDL. It thereby reminded us that the in vitro reconstituted HDL (rHDL) might encountered with oxidation-induced dysfunction. Accordingly, a green-inspired method was employed to recycle non-split HDL from human plasma fraction IV. Then it was compared with rHDL formulated by an ethanol-injection method in terms of physicochemical properties and anti-dysfunctional activities. Results exhibited that rHDL oxidation extent exceeded that of non-split HDL evidenced by higher malondialdehy content, weaker inhibition on low-density lipoprotein (LDL) oxidation and more superoxide anion. The reserved paraoxonase-1 activity on non-split HDL could partially explain for above experimental results. In the targeted transport mechanism experiment, upon SR-BI receptor inhibition and/or CD36 receptor blockage, the almost unchanged non-split HDL uptake in lipid-laden macrophage indicated its negligible oxidation modification profile with regard to rHDL again. Furthermore, compared to rHDL, better macrophage biofunctions were observed for non-split HDL as illustrated by accelerated cholesterol efflux, inhibited oxidized LDL uptake and lessened cellular lipid accumulation. Along with decreased ROS secretion, obviously weakened oxidative stress damage was also detected under treatment with non-split HDL. More importantly, foam cells with non-split HDL-intervention inspired an enhanced inflammation repression and apoptosis inhibition effect. Collectively, the anti-dysfunctional activities of non-split HDL make it suitable as a potential nanocarrier platform for cardiovascular drug payload and delivery.

Midbrain dopamine (DA) neurons are associated with locomotor and psychiatric disorders. DA phenotype is specified in ancestral neural precursor cells (NPCs) and maintained throughout neuronal differentiation. Here we show that endogenous expression of MeCP2 coincides with DA phenotype specification in mouse mesencephalon, and premature expression of MeCP2 prevents in vitro cultured NPCs from acquiring DA phenotype through interfering NURR1 transactivation of DA phenotype genes. By contrast, ectopic MeCP2 expression does not disturb DA phenotype in the DA neurons. By analyzing the dynamic change of DNA methylation along DA neuronal differentiation at the promoter of DA phenotype gene tyrosine hydroxylase (Th), we show that Th expression is determined by TET1-mediated de-methylation of NURR1 binding sites within Th promoter. Chromatin immunoprecipitation assays demonstrate that premature MeCP2 dominates the DNA binding of the corresponding sites thereby blocking TET1 function in DA NPCs, whereas TET1-mediated de-methylation prevents excessive MeCP2 binding in DA neurons. The significance of temporal DNA methylation status is further confirmed by targeted methylation/demethylation experiments showing that targeted de-methylation in DA NPCs protects DA phenotype specification from ectopic MeCP2 expression, whereas targeted methylation disturbs phenotype maintenance in MeCP2-overexpressed DA neurons. These findings suggest the appropriate timing of MeCP2 expression as a novel determining factor for guiding NPCs into DA lineage.

The nanotube-consisted flower-like NiCo2S4 is successfully fabricated by a novel two-step hydrothermal technique. X-ray diffraction (XRD) identifies the spinel structure, scanning electron microscopy (SEM) and transmission electron microscopy (TEM) imply the flower-like morphology of the synthesized NiCo2S4. The electrochemical behaviors are studied by cyclic voltammetry and galvanostatic charge-discharge measurements. The NiCo2S4 nanotubes demonstrate enhanced pseudocapacitive performance of 429.5 C g-1 at current density of 0.5 A g-1. The NiCo2S4//AC device delivers high energy density of 37.69 Wh kg-1, maximum power density of 4000.6 W kg-1 and satisfied cycle property of 96% capacitance retention after over 7000 cycles. The results show that the NiCo2S4 nanotubes are promising electrode material for high performance supercapacitor applications.

Motherhood protection means that the government provides protective measures to help mothers during pregnancy and childbirth. During Taisho period, there was a heated discussion over motherhood protection among feminists. The leading feminists in this discussion, Hiratsuka Raicho and Yosano Akiko, held totally opposed ideas towards motherhood protection. It is worth noting that the differences in their views are mainly due to their different life experiences. This heated debate lasted for several years, although it did not reach a concrete conclusion, it left a valuable legacy for later generations of feminists. Analyzing the reasons for their different attitudes and exploring the theoretical logic behind their different attitudes can provide lessons for solving the problems of low fertility and motherhood protection in Japan and the world today. To truly protect women's interest and promote gender equality, motherhood protection should focuses more on the plight of women due to childbirth than national interest, making becoming a mother no longer be an obstacle to women's self-achievement and personal independence.

There are still many challenges in understanding the absorption and transport mechanism of liposomes in the gastrointestinal tract of infants, especially for liposome-coentrapped two or more substances. In this study, novel docosahexaenoic acid (DHA)-anthocyanidin-codelivery liposomes (DA-LPs) were fabricated and characterized, and their digestive and absorptive behaviors were evaluated using the in vitro infant digestive method combined with the Caco-2 cell model. The liposomal bilayer structure remained intact with the particles aggregated in simulated infant gastric fluid, while their phospholipid membrane underwent enzymatic lipolysis under simulated intestinal conditions. Compared to single substance-loaded liposomes (DHA- or anthocyanidin-loaded liposomes), the digested DA-LPs showed better cell viability, higher cellular uptake and membrane fluidity, and lower reactive oxygen species (ROS). It can be concluded that DA-LPs are promising carriers for simultaneously transporting hydrophobic and hydrophilic molecules and may be beneficial for improving nutrient absorption and alleviating intestinal stress oxidation.

NiS/NiO nanoparticles are successfully fabricated through a simple dealloying method and an ion-exchange process. X-ray diffraction demonstrates the existence of NiO and NiS phases, and scanning electron microscopy and transmission electron microscopy imply the nanopore distribution nature and the nanoparticle morphology of the produced material. The electrochemical behaviors are studied by cyclic voltammetry and galvanostatic charge-discharge measurements. The NiS/NiO electrode shows an enhanced specific capacitance of 1260 F g-1 at a current density of 0.5 A g-1. The NiS/NiO//AC device provides a maximum energy density of 17.42 W h kg-1, a high power density of 4000 W kg-1, and a satisfactory cycling performance of 93% capacitance retention after 30,000 cycles.

Two groups of polymer dispersed liquid crystals (PDLCs) have been studied, results indicated ,when the liquid crystals (LCs) and the UV monomers before curing in the preparation had poor miscibilit...

Polymer dispersed liquid crystal (PDLC) films have been extensively studied for their ability to control the attitude of aircraft by varying the reflectivity of solar sails. However, most of the po...

A composite film with multiple anti-counterfeiting features was demonstrated by superposing quantum dots (QDs) polymer matrix (film A) and cholesteric liquid crystal film (film B) together. The first-line and second-line anti-counterfeiting characteristics were successfully implemented by employing thermochromic, angular photochromic, and circularly polarized discoloration of film B, respectively. By initiatively utilizing the different relative positions between the fluorescence emission peak (λem) of film A and the central selective reflection wavelength (λm) of film B at different temperatures, which resulted in changes in the fluorescence spectra or the different presence of latent patterns, the most important third-line anti-counterfeiting feature was successfully achieved.

In the current complex economic situation, production enterprises are facing manyuncertainties. Reasonable planning of the ordering and transportation of raw materials to ensure the stable operation of the production supply chain is the key to the sustainable development of enterprises. In this paper, a TOPSIS model based on entropy weight and a raw material ordering and transportation model for production enterprises based on multi-objective programming are proposed to provide reference for raw material ordering and transportation selection of production enterprises. In this model, the supply characteristics of suppliers are quantitatively analyzed, and on the basis of the demand of production enterprises, the supplier is determined based on the principle of minimum number of suppliers. Then, the future transshipment loss rate of the transporter is predicted based on the time series, and then the corresponding transporter and transshipment scheme are determined with the lowest loss rate as the target. The research on the model of suppliers and transporter has a good guiding significance for the ordering and transportation of raw materials in manufacturing enterprises.

Objective. To evaluate and summarize the best evidence for nonpharmacological management of chemotherapy induced nausea and vomiting (CINV). This article aims at serving as a reference for nursing staff to prevent and manage CINV. Methods. We systematically searched for evidence on CINV in databases such as Cnki and Wanfang, guide websites such as GIN and NZGG, and professional websites such as UICC and ACS. Only articles published between November 2012 and November 2021 were considered for inclusion in the summary. Two researchers evaluated the quality of the literature and extracted the data. Results. A total of 22 articles were included in this study, including 3 clinical decisions, 2 guidelines, 1 evidence summary, 2 recommended practices, 13 systematic reviews, and 1 expert consensus. Finally, 15 pieces of evidence were summarized from 3 perspectives: risk factor assessment, nursing process specification, and nonpharmacological interventions. Conclusion. Our research summarizes the best evidence on nonpharmacological management of CINV. During actual clinical application, it is necessary to fully consider the clinical situation, combine professional judgment with patients’ wishes, follow the principle of individualization, analyze the obstacles and facilitating factors of the application of evidence, and prudently apply the evidence to the clinical practice.

Midbrain dopamine (DA) neurons are associated with locomotor and psychiatric disorders. DA subtype is specified in ancestral neural precursor cells (NPCs) and maintained throughout neuronal differentiation. Here we show that endogenous expression of MeCP2 coincides with DA subtype specification in mouse mesencephalon, and premature expression of MeCP2 prevents in vitro cultured NPCs from acquiring DA subtype through interfering NURR1 transactivation of DA phenotype genes. By contrast, MeCP2 overexpression does not disturb DA subtype in DA neurons. By analyzing the dynamic change of DNA methylation along DA neuronal differentiation at the promoter of DA phenotype gene tyrosine hydroxylase (Th), we show that Th expression is determined by TET1-mediated de-methylation of NURR1 binding sites within Th promoter. Chromatin immunoprecipitation assays demonstrate that MeCP2 dominates the DNA binding of the corresponding sites thereby blocking TET1 function in DA NPCs, whereas TET1-mediated de-methylation prevents excessive MeCP2 binding in DA neurons. The significance of temporal DNA methylation status is further confirmed by targeted methylation/demethylation experiments showing that targeted de-methylation in DA NPCs protects DA subtype specification from MeCP2 overexpression, whereas targeted methylation disturbs subtype maintenance in MeCP2-overexpressed DA neurons. These findings suggest the appropriate timing of MeCP2 expression as a novel determining factor for guiding NPCs into DA lineage.

There are still many challenges in understanding the absorption and transport mechanism of liposomes in the gastrointestinal tract of infants, especially for liposome-coentrapped two or more substances. In this study, novel docosahexaenoic acid (DHA)-anthocyanidin-codelivery liposomes (DA-LPs) were fabricated and characterized, and their digestive and absorptive behaviors were evaluated using the

In this study, the influence of dietary compounds, including antioxidants (ethylenediaminetetraacid, ascorbic acid, catechin), chitosan and lactoferrin, on the stability of liposomes during in vitro adult and infant digestion was investigated in terms of formation of thiobarbituric acid‐reactive substances (TBARS) and free fatty acid release. Liposomes were more sensitive to degradation in adults than in infants, suggesting lower pH (1.5) and higher concentration of pancreatic enzymes (3.2 mg mL⁻¹) and bile salts (5 mg mL⁻¹) that indicated greater damage to the structure of liposomes. Compared to ethylenediaminetetraacid and ascorbic acid, catechin presented the most apparent protective effect against liposomal oxidation by scavenging free radicals. Chitosan promoted the formation of TBARS, while lactoferrin facilitated the hydrolysis rate of liposomes under both adult and infant conditions. This study provided an insight into the development of healthcare products and functional foods related to liposomes for special populations.

In this paper, we report the design, preparation and properties of epoxy-mercaptan polymer/nematic liquid crystal (LC) composites with electrically switchable optical transmittance. It is found that the polymer microstructure can be regulated from porous structure to microspheres or beads by appropriately modulating the curing temperature, the molecular structure of mercaptan, as well as the compositions of epoxy, mercaptan and nematic LC host. Under an external electric field, the thin films based on such composites can switch from opaque to transparent. The electro-optical performance is found to be highly dependent on the polymer microstructures and the optimum characteristics can be achieved in the composite with a microstructure of smaller microspheres. The possible mechanism for the formation of polymer microspheres and the effects of the microstructures on the electro-optical characteristics of the composites are also discussed. This work is expected to provide an efficient way to control the microstructure of epoxy/mercaptan polymer and develop the composites with switchable optical transmittances for both civil and military applications.

14-3-3 proteins, a family of conserved molecules in eukaryotes, target a number of protein clients through their ability to recognize well-defined phosphorylated motifs. ADF4, as one of Actin-Depolymerizing Factor (ADF) family of proteins, is involved in plant development, and response to biotic and abiotic stresses. Here, we show that 14-3-3κ specially interacted with ADF4 in vitro and in vivo. The 14-3-3κ×adf4 double mutant displayed less F-actin bundle and shorter hypocotyl compared with adf4 mutant, indicating that 14-3-3κ acts upstream of ADF4 to mediate the hypocotyl growth in the dark-grown seedlings. Under the osmotic stress, 14-3-3κ mutants displayed less survival rate than wild-type plants. The adf4 mutants exhibited markedly enhanced survival rate under osmotic treatment, while ADF4-overexpressing plants displayed the opposite results, indicating that ADF4 plays a negative role in response to osmotic stress in Arabidopsis. The interaction between ADF4 and 14-3-3κ inhibited the association of ADF4 with actin filament. Moreover, the in vitro phosphorylation assay demonstrates that the phosphorylation of ADF4 by CASEIN KINASE1-LIKE PROTEIN2 (CKL2) was enhanced by binding 14-3-3κ. Collectively, our data infer a fundamental role for the interaction between 14-3-3κ and ADF4 in regulating hypocotyl growth and osmotic tolerance of plants.

Fabricating functional materials via molecular self-assembly is a promising approach, and precisely controlling the molecular building blocks of nanostructures in the self-assembly process is an essential and challenging task. Blue phase liquid crystals are fascinating self-assembled three-dimensional nanomaterials because of their potential information displays and tuneable photonic applications. However, one of the main obstacles to their applications is their narrow temperature range of a few degrees centigrade, although many prior studies have broadened it to tens via molecular design. In this work, a series of tailored uniaxial rodlike mesogens disfavouring the formation of blue phases are introduced into a blue phase system comprising biaxial dimeric mesogens, a blue phase is observed continuously over a temperature range of 280 °C, and the range remains over 132.0 °C after excluding the frozen glassy state. The findings show that the molecular synergistic self-assembly behavior of biaxial and uniaxial mesogens may play a crucial role in achieving the ultrastable three-dimensional nanostructure of blue phases., Blue phases are spatially ordered yet fragile liquid crystalline structures, bearing applications in optoelectronics and photonics. Hu et al. show that self-assembly within a mixture of different mesogens may significantly broaden the temperature range over which they are stable.

Introduction Contrast-induced nephropathy (CIN) is an major complication of enhanced computed tomography(CT) procedures.Development of CIN after enhanced CT may lead to worse clinical outcomes. Thus, markers are used to identify the risk of patients ,who undergo enhanced CT with CIN.Red cell distribution width(RDW) is a measurement of erythrocyte variability and heterogeneity, and it is obtained routinely in standard complete blood cell counts.Current researches indicate that RDW is the independent predictor of AKI after patients receiving percutaneous coronary intervention (PCI).However, the correlation of RDW and CIN after enhanced CT are yet to be determined.In this study we investigated whether RDW levels correlate with development of CIN in patients after enhanced CT. Method A total of 633 patients undergoing enhanced CT from June 2015 to June 2018 at Huizhou Central Municipal Hospital were enrolled. Patients were divided into CIN group and no-CIN group. The diagnostic criteria for CIN is an increase in serum creatinine(Scr) more than 44.2μmol/L or 25% higher than the baseline value within 72 hours after contrast administration. Results The study population consisted of 633 patients ,25(3.95%) patients developed into CIN. Patients who got CIN were older and had a higher prevalence of active smoking compared with those were without CIN. The range of the baseline serum creatinine and red cell distribution width was significantly higher in CIN groups.However,baseline eGFR,red blood cell counts,hemoglobin and albumin levels were significantly lower in patients who developed CIN(all P Conclusion Increased baseline of RDW, decreased eGFR and lower albumin are independently related with a higher risk of CIN in patients undergoing enhanced CT.RDW is possibly a predictive biomarker for the early diagnosis of CIN.

A silver-mediated free-radical cascade cyclization of isocyanides with potassium alkyl trifluoroborates was developed. The procedure tolerates a series of functional groups, including chloro, bromo, ester, ketone, and amide. Additionally, it is not sensitive to steric hindrance. Thus, this reaction represents a very useful strategy for the production of 6-alkylated phenanthridine derivatives. The addition of alkyl radical to the isonitrile, followed by a radical aromatic cyclization, is involved in this transformation.

In the present work, the graphene oxide/blue phase liquid crystal composites and long aminoalkyl chains functionalized graphene/blue phase liquid crystal composites were prepared by doping the addi...

A rapid and highly efficient method for the radical formation using potassium alkylfluoroborates as radical precursor is devised and developed which conducts under relatively mild condition using silver(I) oxide as the oxidant. The observed silver mirror phenomenon hints at the fact that Ag2O is the real oxidant. This approach effectively overcomes the drawbacks-stringent reaction conditions and poor tolerance of a variety of functional groups.

In this study, a new method to prepare the chiral polymer/cholesteric liquid crystals composite films with broadband reflective capability was reported. A negative dielectric anisotropy liquid crystal material was utilized as the mesogenic solvents and a chiral mesogenic monomer was employed as the chiral dopant. The chiral mesogenic monomer not only induced the liquid crystal molecules to align to form the helical structure, but also as the polymerizable monomer to be crosslinked under UV light to stabilize the pitch gradient in the materials. The UV absorbing dye was used to generate the intensity gradient of UV light and promoted the diffusion of the chiral mesogenic monomer from the backlight side to the light side. The effect of the content of the chiral mesogenic monomer and the intensity of the UV light applied during the preparation process of the composite film on the broadband reflective capability was studied systematically. The chiral polymer/cholesteric liquid crystals composite film that can reflect the light covering the wavelength from 820 nm to 2100 nm was achieved successfully and was used as the functional film in the windows for shielding part of near-infrared light to achieve the thermal management effect of buildings. This work is expected to provide a new method for the preparation of smart windows and thermal management of buildings.