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1. Sensory Detection by the Vomeronasal Organ Modulates Experience-Dependent Social Behaviors in Female Mice

Author

Anne-Charlotte Trouillet, Chantal Moussu, Kevin Poissenot, Matthieu Keller, Lutz Birnbaumer, Trese Leinders-Zufall, Frank Zufall, and Pablo Chamero

Subjects
olfactory, Gαi2, maternal, lordosis, sex preference, kisspeptin, Neurosciences. Biological psychiatry. Neuropsychiatry, RC321-571
Abstract

In mice, social behaviors are largely controlled by the olfactory system. Pheromone detection induces naïve virgin females to retrieve isolated pups to the nest and to be sexually receptive to males, but social experience increases the performance of both types of innate behaviors. Whether animals are intrinsically sensitive to the smell of conspecifics, or the detection of olfactory cues modulates experience for the display of social responses is currently unclear. Here, we employed mice with an olfactory-specific deletion of the G protein Gαi2, which partially eliminates sensory function in the vomeronasal organ (VNO), to show that social behavior in female mice results from interactions between intrinsic mechanisms in the vomeronasal system and experience-dependent plasticity. In pup- and sexually-naïve females, Gαi2 deletion elicited a reduction in pup retrieval behavior, but not in sexual receptivity. By contrast, experienced animals showed normal maternal behavior, but the experience-dependent increase in sexual receptivity was incomplete. Further, lower receptivity was accompanied by reduced neuronal activity in the anterior accessory olfactory bulb and the rostral periventricular area of the third ventricle. Therefore, neural mechanisms utilize intrinsic sensitivity in the mouse vomeronasal system and enable plasticity to display consistent social behavior.

Published
2021
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2. The kisspeptin analog C6 is a possible alternative to PMSG (pregnant mare serum gonadotropin) for triggering synchronized and fertile ovulations in the Alpine goat.

Author

Caroline Decourt, Vincent Robert, Didier Lomet, Karine Anger, Marion Georgelin, Kevin Poissenot, Maria-Teresa Pellicer-Rubio, Vincent Aucagne, and Massimiliano Beltramo

Subjects
Medicine, Science
Abstract

In temperate regions goat's reproduction is seasonal. To obtain year-round breeding, hormonal treatments are currently applied. These treatments usually combine a progesterone analog with the pregnant mare serum gonadotropin (PMSG). However, their use has significant ethical and environmental drawbacks. Therefore, alternative methods to manage reproduction are needed. The discovery that in mammals the neuropeptide kisspeptin is a major positive regulator of hypothalamo-pituitary gonadal axis offered an attractive alternative strategy to control reproduction. We have previously designed a kisspeptin analog, called C6, which offers pharmacological advantages over endogenous kisspeptin. These include a longer lasting effect and enhanced activity following intramuscular injection. In the present work, we evaluated C6 effect on LH and FSH plasma concentrations in the Alpine goat breed and tested whether C6 could replace PMSG to trigger ovulation. An intramuscular injection of C6 (15 nmol/doe) given 24 hours after the end of progestogen treatment induced a surge-like peak of both LH and FSH. This was followed by an increase of progesterone, a hallmark of ovulation induction and corpus luteus formation. These results were obtained at three different time of the year: during the breeding season, the non-breeding season and at the onset of the breeding season. Furthermore, we compared the efficacy of C6 and PMSG to induce fertile ovulations when these treatments are given at the onset of the breeding season and are followed by artificial insemination. The results of this first attempt were extremely promising with gestation rates of 45% and 64% for C6 and PMSG respectively. Pending optimization of the treatment procedure in order to improve efficacy, kisspeptin analogs could be the long sought-after alternative to PMSG.

Published
2019
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3. Population density does not affect seasonal regulation of reproductive physiology in male water voles

Author

Kevin Poissenot, Chantal Moussu, Morgane Brachet, Didier Chesneau, Philippe Chemineau, Anne-Lyse Lainé, Martine Migaud, Nathalie Charbonnel, and Matthieu Keller

Subjects
General Agricultural and Biological Sciences, Agricultural and Biological Sciences (miscellaneous)
Abstract

Most small rodent species display cyclic fluctuations in their population density. The mechanisms behind these cyclical variations are not yet clearly understood. Density-dependent effects on reproductive function could affect these population variations. The fossorial water vole ecotype, Arvicola terrestris , exhibits multi-year cyclical dynamics with outbreak peaks. Here, we monitored different water vole populations over 3 years, in spring and autumn, to evaluate whether population density is related to male reproductive physiology. Our results show an effect of season and inter-annual factors on testis mass, plasmatic testosterone level, and androgen-dependent seminal vesicle mass. By contrast, population density does not affect any of these parameters, suggesting a lack of modulation of population dynamics by population density.

Published
2023
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4. Effect of early postnatal exposure to 17α-ethinylestradiol on female rat reproductive physiology

Author

Pierre Dorfman, Chantal Moussu, Matthieu Keller, Kevin Poissenot, Alain Reymond, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), CEIA/PROTEOMIS, and Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)

Subjects
[SDV.SA]Life Sciences [q-bio]/Agricultural sciences, 030506 rehabilitation, Endocrine disruption, Health, Toxicology and Mutagenesis, medicine.medical_treatment, Physiology, 010501 environmental sciences, Carbohydrate metabolism, Reproductive physiology, Toxicology, 01 natural sciences, 03 medical and health sciences, Ethinylestradiol, medicine, Adverse effect, 0105 earth and related environmental sciences, Estrous cycle, Reproductive function, business.industry, Reproduction, Insulin, Ovary, Anogenital distance, [SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology, Metabolism, 3. Good health, 0305 other medical science, business
Abstract

International audience; Objective 17 alpha-Ethinylestradiol (EE2) is a synthetic compound used as an oral contraceptive in birth control pills. EE2 is a major contaminant in aquatic environments. It is considered an endocrine-disrupting chemical due to its oestrogenic activity. This study investigates the effect of repeated exposure to EE2 at a dose of 1 mu g/kg center dot(body weight)/day on reproductive function and metabolism. Methods Female Sprague-Dawley rats were subcutaneously injected with EE2 at a dose of 1 mu g/kg center dot(body weight)/day from postnatal day 1 until postnatal day 5, which corresponds to a highly sensitive period of neuroendocrine system organisation. Results The anogenital distance, body weight and blood levels of insulin and glucose of EE2-treated female rats were not different from those of control rats. These results indicate that growth and glucose metabolism were unaffected by treatment. In contrast, vaginal opening occurred significantly earlier in EE2-treated females than in control females. In adulthood, these females present an alteration of their oestrous cyclicity, characterised by persistent vaginal oestrus. Moreover, treated females showed altered uterine and ovarian morphology associated with lower circulating levels of oestradiol and progesterone. Conclusion These results indicate precocious onset of puberty and dysfunction of the reproductive organs. Therefore, early postnatal exposure to EE2 at a dose of 1 mu g/kg center dot bw/d has delayed adverse effects on reproductive function.

Published
2021
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5. Photoperiod is involved in the regulation of seasonal breeding in male water voles (Arvicola terrestris)

Author

Joël R. Drevet, Areski Chorfa, Etienne Ramadier, Philippe Chemineau, Adrien Pinot, Hugues Dardente, Etienne Benoit, Kevin Poissenot, Fabrice Saez, Anne-Charlotte Trouillet, Didier Chesneau, Matthieu Keller, Morgane A. A. Brachet, Chantal Moussu, Virginie Lattard, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut Français du Cheval et de l'Equitation [Saumur] (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Génétique, Reproduction et Développement (GReD), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne (UCA), Rongeurs Sauvages, Risques Sanitaires et Gestion des Populations - UR 1233 (RS2GP), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), and Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)

Subjects
Male, Scent gland, Kisspeptin, Physiology, [SDV.IB.IMA]Life Sciences [q-bio]/Bioengineering/Imaging, [SDV]Life Sciences [q-bio], [SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology, 0302 clinical medicine, Seasonal breeder, Rfrp3, media_common, photoperiodism, 0303 health sciences, biology, [SDV.NEU.PC]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Psychology and behavior, Arvicolinae, [SDV.BA]Life Sciences [q-bio]/Animal biology, Reproduction, [SDV.NEU.SC]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Cognitive Sciences, Hypothalamo-pituitary-gonadal axis, Arcuate nucleus, [SDV.SA.SPA]Life Sciences [q-bio]/Agricultural sciences/Animal production studies, Arvicola, Photoperiodism, [SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology, Seasons, endocrine system, media_common.quotation_subject, Photoperiod, Hypothalamus, Zoology, Context (language use), [SDV.SA.ZOO]Life Sciences [q-bio]/Agricultural sciences/Zootechny, [SDV.TOX.TCA]Life Sciences [q-bio]/Toxicology/Toxicology and food chain, Aquatic Science, [SDV.BDLR.RS]Life Sciences [q-bio]/Reproductive Biology/Sexual reproduction, 03 medical and health sciences, [SDV.BA.ZV]Life Sciences [q-bio]/Animal biology/Vertebrate Zoology, Animals, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], Dorsomedial hypothalamic nucleus, Molecular Biology, Ecology, Evolution, Behavior and Systematics, 030304 developmental biology, [SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health, [SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology, biology.organism_classification, Insect Science, Animal Science and Zoology, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, 030217 neurology & neurosurgery, [SDV.EE.IEO]Life Sciences [q-bio]/Ecology, environment/Symbiosis
Abstract

Mammals living at temperate latitudes typically display annual cyclicity in their reproductive activity: births are synchronized when environmental conditions are most favorable. In a majority of these species, day length is the main proximate factor used to anticipate seasonal changes and to adapt physiology. The brain integrates this photoperiodic signal through key hypothalamic structures, which regulate the reproductive axis. In this context, our study aimed to characterize regulations that occur along the hypothalamo–pituitary–gonadal (HPG) axis in male fossorial water voles (Arvicola terrestris, also known as Arvicola amphibius) throughout the year and to further probe the implication of photoperiod in these seasonal regulations. Our monthly field monitoring showed dramatic seasonal changes in the morphology and activity of reproductive organs, as well as in the androgen-dependent lateral scent glands. Moreover, our data uncovered seasonal variations at the hypothalamic level. During the breeding season, kisspeptin expression in the arcuate nucleus (ARC) decreases, while RFRP3 expression in the dorsomedial hypothalamic nucleus (DMH) increases. Our follow-up laboratory study revealed activation of the reproductive axis and confirmed a decrease in kisspeptin expression in males exposed to a long photoperiod (summer condition) compared with those maintained under a short photoperiod (winter condition) that retain all features reminiscent of sexual inhibition. Altogether, our study characterizes neuroendocrine and anatomical markers of seasonal reproductive rhythmicity in male water voles and further suggests that these seasonal changes are strongly impacted by photoperiod.

Published
2021
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6. Field study reveals morphological and neuroendocrine correlates of seasonal breeding in female water voles, Arvicola terrestris

Author

Fabrice Saez, Adrien Pinot, Matthieu Keller, Etienne Ramadier, Philippe Chemineau, Virginie Lattard, Joël R. Drevet, Chantal Moussu, Rami Abi Khalil, Areski Chorfa, Kevin Poissenot, Etienne Benoit, Hugues Dardente, Didier Chesneau, Yves Michelin, Territoires (Territoires), AgroParisTech-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Clermont Auvergne (UCA), Rongeurs Sauvages, Risques Sanitaires et Gestion des Populations - UR 1233 (RS2GP), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut Français du Cheval et de l'Equitation [Saumur] (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Unité Mixte de Recherche sur l'Ecosystème Prairial - UMR (UREP), Génétique, Reproduction et Développement (GReD), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne (UCA), and AgroParisTech-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Clermont Auvergne (UCA)

Subjects
0106 biological sciences, Scent gland, Kisspeptin, Photoperiod, [SDV]Life Sciences [q-bio], Ovary (botany), Hypothalamus, Zoology, [SDV.TOX.TCA]Life Sciences [q-bio]/Toxicology/Toxicology and food chain, Biology, Seasonal breeding, 010603 evolutionary biology, 01 natural sciences, 03 medical and health sciences, Endocrinology, Arcuate nucleus, medicine, Animals, Water vole, Arvicola terrestris, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], ComputingMilieux_MISCELLANEOUS, 030304 developmental biology, Water voles, photoperiodism, Hypothalamo-pituitary-gonadal axisKisspeptin, 0303 health sciences, [SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health, Arvicolinae, Fossorial, Field study, Hypothalamo-pituitary-gonadal axis, Seasonality, medicine.disease, biology.organism_classification, Neurosecretory Systems, Animal Science and Zoology, Female, [SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology, Seasons, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition
Abstract

International audience; Seasonally breeding mammals display timely physiological switches between reproductive activity and sexual rest, which ensure synchronisation of births at the most favourable time of the year. These switches correlate with seasonal changes along the hypothalamo-pituitary-gonadal axis, but they are primarily orchestrated at the hypothalamic level through environmental control of KISS1-dependent GnRH release. Our field study shows that births of fossorial water voles, Arvicola terrestris, are concentrated between March and October, which indicates the existence of an annual reproductive cycle in this species. Monthly field monitoring for over a year further reveals dramatic seasonal changes in the morphology of the ovary, uterus and lateral scent glands, which correlate with the reproductive status. Finally, we demonstrate seasonal variation in kisspeptin expression within the hypothalamic arcuate nucleus. Altogether, this study demonstrates a marked rhythm of seasonal breeding in the water vole and we speculate that this is governed by seasonal changes in photoperiod.

Published
2021
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7. Effects of combined exposure of adult male mice to di-(2-ethylexyl)phthalate and nonylphenol on behavioral and neuroendocrine responses

Author

Isabelle Franceschini, Kevin Poissenot, Daphné Capela, Carlos Dombret, Matthieu Keller, Sakina Mhaouty-Kodja, Neuroscience Paris Seine (NPS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut de Biologie Paris Seine (IBPS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Institut de Biologie Paris Seine (IBPS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Centre National de la Recherche Scientifique (CNRS)-Université de Tours-Institut Français du Cheval et de l'Equitation [Saumur]-Institut National de la Recherche Agronomique (INRA), Université de Tours, Institut Français du Cheval et de l'Equitation [Saumur], Neurosciences Paris Seine (NPS), Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Biologie Paris Seine (IBPS), Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Neuroplasticité des comportements de reproduction = Neuroplasticity of Reproductive Behaviors (NPS-11), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Biologie Paris Seine (IBPS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Biologie Paris Seine (IBPS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Biologie Paris Seine (IBPS), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Biologie Paris Seine (IBPS), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Institut de Biologie Paris Seine (IBPS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Institut de Biologie Paris Seine (IBPS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC), Gestionnaire, Hal Sorbonne Université, and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur] (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Male, [SDV]Life Sciences [q-bio], [SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology, Health, Toxicology and Mutagenesis, 0208 environmental biotechnology, Estrogen receptor, 02 engineering and technology, 010501 environmental sciences, 01 natural sciences, Mice, Sexual Behavior, Animal, chemistry.chemical_compound, Kisspeptin, Phthalates, Testosterone, Receptor, ComputingMilieux_MISCELLANEOUS, Kisspeptins, Reproduction, Phthalate, General Medicine, Pollution, [SDV.TOX] Life Sciences [q-bio]/Toxicology, Endocrine disruptor, [SDV.TOX]Life Sciences [q-bio]/Toxicology, Environmental Pollutants, Female, [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], Nervous system, medicine.medical_specialty, Environmental Engineering, medicine.drug_class, Biology, Phenols, Diethylhexyl Phthalate, Internal medicine, medicine, Animals, Environmental Chemistry, 0105 earth and related environmental sciences, Behavior, [SDV.NEU.NB] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology, Public Health, Environmental and Occupational Health, General Chemistry, Androgen, Neurosecretory Systems, Nonylphenol, 020801 environmental engineering, Mice, Inbred C57BL, Endocrinology, chemistry
Abstract

International audience; The present study evaluates the effects of adult exposure to low doses of a mixture of di-(2-ethylexyl)phthalate (DEHP) and nonylphenol (NP) on reproductive neuroendocrine function and behavior. The neural circuitry that processes male sexual behavior is tightly regulated by testosterone and its neural metabolite estradiol. In previous studies, we showed that adult exposure of mice to low doses of each of these widespread environmental contaminants resulted in altered sexual behavior, without any effect on the regulation of the gonadotropic axis. Here, adult C57BL/6J male mice were exposed to DEHP/NP (0.5 or 5 μg/kg body weight/day) for 4 weeks before starting the analyses. Mice treated with DEHP/NP at 0.5 μg/kg/day show altered olfactory preference, and fewer of them emit ultrasonic vocalization compared to the other treatment groups. These mice also exhibit a lower number of mounts and thrusts, increased locomotor activity and unaffected anxiety-state level, along with unaltered testosterone levels and kisspeptin system, a key regulator of the gonadotropic axis. Analysis of the neural circuitry that underlies sexual behavior showed that the number of cells expressing androgen and estrogen receptors is comparable between control and DEHP/NP-exposed males. The comparison of these data with those obtained in males exposed to each molecule separately highlights synergistic effects at the lower dose of contaminants of 0.5 μg/kg/day. In contrast, the effects previously observed for each molecule at 5 μg/kg/day were not detected. A detailed comparison of the effects triggered by separate or combined exposure to DEHP and NP is discussed.

Published
2019
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8. No evidence that Spexin impacts LH release and seasonal breeding in the ewe

Author

Didier Lomet, Vincent Robert, Kevin Poissenot, Massimiliano Beltramo, Hugues Dardente, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Centre National de la Recherche Scientifique (CNRS)-Université de Tours-Institut Français du Cheval et de l'Equitation [Saumur]-Institut National de la Recherche Agronomique (INRA), Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), and Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)

Subjects
medicine.medical_specialty, Kisspeptin, Photoperiod, [SDV]Life Sciences [q-bio], Hypothalamus, Peptide hormone, Biology, Breeding, Spexin, 03 medical and health sciences, [SCCO]Cognitive science, 0302 clinical medicine, Food Animals, Complementary DNA, Internal medicine, medicine, Animals, Small Animals, Gene, ComputingMilieux_MISCELLANEOUS, 030304 developmental biology, chemistry.chemical_classification, Kisspeptins, 0303 health sciences, Sheep, Estradiol, Equine, Thyroid, [SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology, Seasonality, Kiss1, Amino acid, medicine.anatomical_structure, Endocrinology, chemistry, Pituitary, GnRH, Female, Animal Science and Zoology, Seasons, 030217 neurology & neurosurgery, Hormone
Abstract

International audience; Spexin (SPX) is a recently identified peptide hormone of 14 amino acids. Interestingly, Spx and Kiss1 genes share a common ancestor gene. Considering that KISS1 peptides are key controllers of breeding in mammals and circumstantial evidence that SPX regulates gonadotropins in some fish species, we hypothesized that SPX may play a KISS1-related role in sheep. Here, we cloned the ovine Spx cDNA, performed in vivo injection and infusion of SPX (i.c.v. route, with or without concomittant KISS1 presence) and assessed a potential regulation of Spx expression by season, thyroid hormone and estradiol in the medio-basal hypothalamus of the ewe. Our data do not provide support for a role of SPX in the control of the gonadotropic axis in the ewe.

Published
2020
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9. Brain mapping of the gonadotropin‐inhibitory hormone‐related peptide 2 with a novel antibody suggests a connection with emotional reactivity in the Japanese quail ( Coturnix japonica , Temminck & Schlegel, 1849)

Author

Fabien Cornilleau, Hugues Dardente, Karine Anger, Kevin Poissenot, Paul Constantin, Massimiliano Beltramo, Ludovic Calandreau, Didier Lomet, Kazuyoshi Tsutsui, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Pôle d'Expérimentation Avicole de Tours (UE PEAT), Institut National de la Recherche Agronomique (INRA), Waseda University, and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)

Subjects
0301 basic medicine, endocrine system, medicine.medical_specialty, Peptide Hormones, [SDV]Life Sciences [q-bio], Emotions, Neuropeptide, emotional reactivity, GnIH, GnIH-RP2, quail, reproduction, RFRP-3, stress response, Coturnix, Antibodies, 03 medical and health sciences, Diencephalon, 0302 clinical medicine, Internal medicine, biology.animal, medicine, Animals, [INFO]Computer Science [cs], ComputingMilieux_MISCELLANEOUS, Brain Mapping, [SDV.GEN]Life Sciences [q-bio]/Genetics, biology, Cerebrum, General Neuroscience, Coturnix japonica, Brain, biology.organism_classification, Immunohistochemistry, Quail, [SDV.GEN.GA]Life Sciences [q-bio]/Genetics/Animal genetics, Stria terminalis, 030104 developmental biology, Endocrinology, medicine.anatomical_structure, nervous system, Hypothalamus, Nucleus, 030217 neurology & neurosurgery
Abstract

Gonadotropin-inhibitory hormone (GnIH) is a neuropeptide first discovered in the quail brain that is involved in the control of reproductive physiology and behaviors, and stress response. GnIH gene encodes a second peptide, GnIH-related peptide-2 (RP2), the distribution and function of which remain unknown. We therefore studied GnIH-RP2 distribution by immunohistochemistry using a novel antibody capable of discriminating between GnIH and GnIH-RP2. The overall distribution of GnIH-RP2 is similar to that of GnIH. The vast majority of labeled neurons is located in the paraventricular nucleus (PVN) of the hypothalamus. Labeling of fibers is conspicuous in the diencephalon, but present also in the mesencephalon and telencephalon. Several regions involved in the control of reproduction and stress response (the PVN, septum, bed nucleus of the stria terminalis and nucleus commissura pallii) showed a dense network of immunolabeled fibers. To investigate the potential function of GnIH-RP2 we compared its expression in two quail lines genetically selected for divergence in their emotional reactivity. A quantitative analysis in the above-mentioned brain regions showed that the density of fibers was similar in the two lines. However, the number of GnIH-RP2 labeled neurons was higher in the median portion of the PVN in birds with higher emotional reactivity. These results point to a possible involvement of GnRH-RP2 in modulating stress response and/or emotional reactivity.

Published
2019
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10. Seasonal diet-based resistance to anticoagulant rodenticides in the fossorial water vole (Arvicola amphibius)

Author

Adrien Pinot, Etienne Benoit, Ali Barka Mahamat, Ambre Fafournoux, Virginie Lattard, Matthieu Keller, Michael Coeurdassier, Brigitte Barbier, Aurélie Marquez, Javier Fernandez-de-Simon, Rami Abi Khalil, Sébastien Lefebvre, Kevin Poissenot, Amélie Desvars-Larrive, Rongeurs Sauvages, Risques Sanitaires et Gestion des Populations - UR 1233 (RS2GP), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), University of Veterinary Medicine [Vienna] (Vetmeduni), Complexity Science Hub Vienna (CSHV), Laboratoire Chrono-environnement - CNRS - UBFC (UMR 6249) (LCE), Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), Service de nutrition et de bromatologie animale (SNBA-VAS), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS), and Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)

Subjects
Diet-based resistance, Vitamin, Vitamin K, Rodent, Zoology, [SDV.TOX.TCA]Life Sciences [q-bio]/Toxicology/Toxicology and food chain, 010501 environmental sciences, 01 natural sciences, Biochemistry, Mice, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Vitamin K Epoxide Reductases, biology.animal, Animals, 030212 general & internal medicine, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], Water vole, Allele frequency, 0105 earth and related environmental sciences, General Environmental Science, 2. Zero hunger, Herbivore, [SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health, biology, Resistance (ecology), Arvicolinae, Fossorial, Anticoagulants, Membrane Proteins, Rodenticides, biology.organism_classification, Diet, Rats, Anticoagulant rodenticides, VKORC1, chemistry, Arvicola, Seasons, [SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition
Abstract

International audience; Anticoagulant rodenticides (AR) resistance has been defined as "a major loss of efficacy due to the presence of a strain of rodent with a heritable and commensurately reduced sensitivity to the anticoagulant". The mechanism that supports this resistance has been identified as based on mutations in the Vkorc1 gene leading to severe resistance in rats and mice. This study evaluates the validity of this definition in the fossorial water vole and explores the possibility of a non-genetic diet-based resistance in a strict herbivorous rodent species. Genetic support was explored by sequencing the Vkorc1 gene and the diet-based resistance was explored by the dosing of vitamins K in liver of voles according to seasons. From a sample of 300 voles, only 2 coding mutations, G71R and S149I, were detected in the Vkorc1 gene in the heterozygous state with low allele frequencies (0.5-1%). These mutations did not modify the sensitivity to AR, suggesting an absence of genetic Vkorc1-based resistance in the water vole. On the contrary, vitamin K1 was shown to be 5 times more abundant in the liver of the water vole compared to rats. This liver concentration was shown to seasonally vary, with a trough in late winter and a peak in late spring/early summer related to the growth profile of grass. This increase in concentration might be responsible for the increased resistance of water voles to AR. This study highlights a non-genetic, diet-related resistance mechanism in rodents to AR. This diet-based resistance might explain the different evolution of the Vkorc1 gene in the fossorial water vole compared to rats and mice.

Published
2021
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11. Sexually dimorphic gene expression and neurite sensitivity to estradiol in fetal arcuate Kiss1 cells

Author

Kevin Poissenot, William H. Colledge, Daniel Guillaume, Yves Levern, Vincent Robert, Isabelle Franceschini, Caroline Alfaia, Shel-Hwa Yeo, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Infectiologie et Santé Publique (UMR ISP), Université de Tours-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Reproductive Physiology Group, Department of Physiology, Development, and Neuroscience, University of Cambridge [UK] (CAM), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université de Tours (UT)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), BBRC (BB/K003178/) University of ToursFondation pour la Recherche Medicale (FDT20160736471), ANR-15-CE20-0015,Kiss,Developpement d'analogues de la kisspeptine pour le contrôle de la reproduction(2015), and Institut Français du Cheval et de l'Equitation [Saumur] (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)

Subjects
0301 basic medicine, Male, medicine.medical_specialty, Neurite, medicine.drug_class, Neurokinin B, Endocrinology, Diabetes and Metabolism, [SDV]Life Sciences [q-bio], Population, 030209 endocrinology & metabolism, Biology, 03 medical and health sciences, chemistry.chemical_compound, Mice, 0302 clinical medicine, Endocrinology, Kisspeptin, Internal medicine, medicine, Neurites, Animals, education, ComputingMilieux_MISCELLANEOUS, education.field_of_study, Kisspeptins, Sex Characteristics, Sexual differentiation, Arc (protein), Estradiol, Arcuate Nucleus of Hypothalamus, Gene Expression Regulation, Developmental, Mice, Inbred C57BL, 030104 developmental biology, chemistry, Estrogen, Female, GPER
Abstract

Kiss1 neurons of the arcuate (ARC) nucleus form an interconnected network of cells that communicate via neurokinin B (encoded by Tac2) and its receptor (encoded by Tacr3) and play key roles in the control of the reproductive axis through sex hormone-regulated synthesis and release of kisspeptin peptides (Kp, encoded by Kiss1). The aim of this study was to determine whether the Kiss1 cell population of the ARC already displays sexually dimorphic features at embryonic age E16.5 in mice. At this time of development, Kiss1-GFP- and Kp-immunoreactive cell bodies were restricted to the ARC and not found in the pre-optic area (POA). The Kiss1-GFP cell population was identical in size between sexes but had significantly lower Kiss1, Tac2, and Tacr3 mRNA levels and lower Kp-ir fiber density in the POA in male compared to female fetuses. Receptors for androgen (Ar) and estrogen (Esr1, Esr2, Gpr30) and the Cyp19a1 gene (encoding the estradiol-producing enzyme aromatase) transcripts were also detected in fetal ARC Kiss1-GFP cells with significant sex differences for Ar (higher in males) and Esr1 (higher in females). Functional studies on primary cultures of sorted fetal Kiss1-GFP cells revealed a significant negative effect of estradiol treatment on neurite outgrowth on the fourth day of culture in the female group specifically. We conclude that the ARC Kiss1 cell population is already sexually differentiated at E16.5 and that its morphogenetic development may be particularly vulnerable to estradiol exposure at this early developmental time.

Published
2020
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12. The kisspeptin analog C6 is a possible alternative to PMSG (pregnant mare serum gonadotropin) for triggering synchronized and fertile ovulations in the Alpine goat

Author

Maria-Teresa Pellicer-Rubio, Massimiliano Beltramo, Marion Georgelin, Caroline Decourt, Kevin Poissenot, Vincent Robert, Vincent Aucagne, Karine Anger, Didier Lomet, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Centre de biophysique moléculaire (CBM), Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), ANR-15-CE20-0015,Kiss,Developpement d'analogues de la kisspeptine pour le contrôle de la reproduction(2015), Centre National de la Recherche Scientifique (CNRS)-Université de Tours-Institut Français du Cheval et de l'Equitation [Saumur]-Institut National de la Recherche Agronomique (INRA), LEGOUPIL, Laëtitia, Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur] (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Gonadotropins, Equine, Physiology, medicine.medical_treatment, [SDV]Life Sciences [q-bio], Pregnant Mare Serum Gonadotropin, Progesterone analog, Biochemistry, [SCCO]Cognitive science, Kisspeptin, Endocrinology, Reproductive Physiology, Biologie de la reproduction, Medicine and Health Sciences, Lipid Hormones, Animal Husbandry, Progesterone, Routes of Administration, media_common, Animal Management, Animal biology, Mammals, Reproductive Biology, 0303 health sciences, Kisspeptins, Multidisciplinary, Goats, Reproduction, Eukaryota, Agriculture, synchronisation de l'ovulation, 04 agricultural and veterinary sciences, Ruminants, Body Fluids, [SDV] Life Sciences [q-bio], Blood, Vertebrates, Medicine, Female, Anatomy, hormones, hormone substitutes, and hormone antagonists, Research Article, Ovulation, chèvre, media_common.quotation_subject, Science, saison de reproduction, Biology, Insemination, Blood Plasma, Andrology, 03 medical and health sciences, réponse hormonale, période de fertilité, Biologie animale, medicine, Animals, Intramuscular Injections, Menstrual Cycle, 030304 developmental biology, Pharmacology, Progestogen, Endocrine Physiology, kisspeptine, Artificial insemination, 0402 animal and dairy science, Organisms, Biology and Life Sciences, Luteinizing Hormone, 040201 dairy & animal science, Hormones, Fertility, Fertilization, Amniotes, Ovulation induction, Follicle Stimulating Hormone, Gonadotropins, Developmental Biology
Abstract

International audience; In temperate regions goat's reproduction is seasonal. To obtain year-round breeding, hormonal treatments are currently applied. These treatments usually combine a progesterone analog with the pregnant mare serum gonadotropin (PMSG). However, their use has significant ethical and environmental drawbacks. Therefore, alternative methods to manage reproduction are needed. The discovery that in mammals the neuropeptide kisspeptin is a major positive regulator of hypothalamo-pituitary gonadal axis offered an attractive alternative strategy to control reproduction. We have previously designed a kisspeptin analog, called C6, which offers pharmacological advantages over endogenous kisspeptin. These include a longer lasting effect and enhanced activity following intramuscular injection. In the present work, we evaluated C6 effect on LH and FSH plasma concentrations in the Alpine goat breed and tested whether C6 could replace PMSG to trigger ovulation. An intramuscular injection of C6 (15 nmol/doe) given 24 hours after the end of progestogen treatment induced a surge-like peak of both LH and FSH. This was followed by an increase of progesterone, a hallmark of ovulation induction and corpus luteus formation. These results were obtained at three different time of the year: during the breeding season, the non-breeding season and at the onset of the breeding season. Furthermore, we compared the efficacy of C6 and PMSG to induce fertile ovulations when these treatments are given at the onset of the breeding season and are followed by artificial insemination. The results of this first attempt were extremely promising with gestation rates of 45% and 64% for C6 and PMSG respectively. Pending optimization of the treatment procedure in order to improve efficacy, kisspeptin analogs could be the long sought-after alternative to PMSG.

Published
2019
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13. Sexually active bucks are a critical social cue that activates the gonadotrope axis and early puberty onset in does

Author

Massimiliano Beltramo, Matthieu Keller, Manon Chasles, Chantal Moussu, Didier Chesneau, Philippe Chemineau, Kevin Poissenot, José Alberto Delgadillo, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Centro de Investigacion en Reproduccion Caprina, Universidad Autónoma Agraria Antonio Narro (UAAAN), and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)

Subjects
0301 basic medicine, Male, Ovulation, media_common.quotation_subject, Uterus, Physiology, Biology, Gonadotropic cell, Social interactions, Anestrus, Gonadotropin-Releasing Hormone, 03 medical and health sciences, Behavioral Neuroscience, Sexual Behavior, Animal, Endocrinology, Kisspeptin, Vandenbergh effect, medicine, Seasonal breeder, Weaning, Animals, Sexual Maturation, Social Behavior, media_common, Behavior, Endocrine and Autonomic Systems, Goats, [SDV.BA]Life Sciences [q-bio]/Animal biology, INT, Puberty, Genital tract, [SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology, 030104 developmental biology, medicine.anatomical_structure, GnRH, LH pulsatility, Female, Seasons, Cues
Abstract

International audience; In rodents, early exposure to adult male is well known to induce an early puberty in females (Vandenbergh effect). This phenomenon has been less studied in other mammals. In goats, despite our extensive knowledge about the "male-effect" phenomenon in adults (i.e. ovulation induced by the introduction of the male during the anestrous), there are few data on the consequences of an early exposure of females to males. Here, we evaluated the puberty onset of young alpine goats when raised since weaning with intact bucks (INT), with castrated bucks (CAS) or isolated from bucks (ISOL). The INT group had the first ovulation 1.5 month before the two other groups. Despite the earlier puberty the INT group of females had normal and regular ovarian cycles. Morphological study of the genital tract showed that at 6 months, uterus of INT goats was 40% heavier than CAS and ISOL goats. Moreover, INT females had a myometrium significantly thicker and INT was the only group having corpora lutea. In our study, INT females were pubescent in the month following the entry of bucks into the breeding season, suggesting that only sexually active bucks provide the signal responsible for puberty acceleration. By removing direct contact with the bucks, we showed that somatosensory interactions were dispensable for an early puberty induction. Finally, no difference in the GnRH network (fiber density and number of synaptic appositions) can be detected between pubescent and non-pubescent females, suggesting that the male stimulations triggering puberty onset act probably on upstream neuronal networks, potentially on kisspeptin neurons.

Published
2018
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14. Short photoperiod modulates behavior, cognition and hippocampal neurogenesis in male Japanese quail

Author

Marion Georgelin, Vitor Hugo Bessa Ferreira, Fabien Cornilleau, Maryse Meurisse, Kévin Poissenot, Massimiliano Beltramo, Matthieu Keller, Léa Lansade, Hugues Dardente, and Ludovic Calandreau

Subjects
Medicine, Science
Abstract

Abstract The mechanisms underlying the photoperiodic control of reproduction in mammals and birds have been recently clarified. In contrast, the potential impact of photoperiod on more complex, integrative processes, such as cognitive behaviors, remains poorly characterized. Here, we investigated the impact of contrasted long and short photoperiods (LP, 16 h light/day and SP, 8 h light/day, respectively) on learning, spatial orientation abilities, and emotional reactivity in male Japanese quail. In addition, we quantified cell proliferation and young cell maturation/migration within the hippocampus, a brain region involved in spatial orientation. Our study reveals that, in male quail, SP increases emotional responses and spatial orientation abilities, compared to LP. Behaviorally, SP birds were found to be more fearful than LP birds, exhibiting more freezing in the open field and taking longer to exit the dark compartment in the emergence test. Furthermore, SP birds were significantly less aggressive than LP birds in a mirror test. Cognitively, SP birds were slower to habituate and learn a spatial orientation task compared to LP birds. However, during a recall test, SP birds performed better than LP birds. From a neuroanatomical standpoint, SP birds had a significantly lower density of young neurons, and also tended to have a lower density of mature neurons within the hippocampus, compared to LP birds. In conclusion, our data reveal that, beyond breeding control, photoperiod also exerts a profound influence on behavior, cognition, and brain plasticity, which comprise the seasonal program of this species.

Published
2023
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15. Neural mechanisms underlying the disruption of male courtship behavior by adult exposure to Di(2-ethylhexyl) phthalate in mice

Author

Solenne Chardonnet, Valérie Grange-Messent, Carlos Dombret, Isabelle Franceschini, Matthieu Keller, Cédric Pionneau, Daphné Capela, Sakina Mhaouty-Kodja, Kevin Poissenot, Caroline Parmentier, Emma Bergsten, Hélène Hardin-Pouzet, ProdInra, Archive Ouverte, Institut de Biologie Paris Seine (IBPS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Neuroscience Paris Seine (NPS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut de Biologie Paris Seine (IBPS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut Français du Cheval et de l'Equitation [Saumur] (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Plateforme Post-génomique de la Pitié-Salpêtrière (PASS-P3S), Unité Mixte de Service Production et Analyse de données en Sciences de la vie et en Santé (PASS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Université de Tours-Institut Français du Cheval et de l'Equitation [Saumur]-Institut National de la Recherche Agronomique (INRA), Early detection of Colon Cancer using Molecular Markers and Microbiota (EA 7375) (EC2M3), Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Plateforme Post-génomique de la Pitié-Salpêtrière (P3S), UMS omique (OMIQUE), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Sorbonne Universités, Université Pierre et Marie Curie (Paris 6), Institut National de la Santé et de la Recherche Médicale (INSERM), French Agency for Food, Environmental and Occupational Health & Safety (Anses, Project No 2012-2-077), Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Neurosciences Paris Seine (NPS), Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Biologie Paris Seine (IBPS), Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), and Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université de Tours-Institut Français du Cheval et de l'Equitation [Saumur]

Subjects
Male, 0301 basic medicine, [SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT], endocrine system, [SDV.BIO]Life Sciences [q-bio]/Biotechnology, [SDV]Life Sciences [q-bio], Health, Toxicology and Mutagenesis, media_common.quotation_subject, Zoology, Endocrine Disruptors, Biology, souris, in vitro growth, Courtship, Toxicology, Mice, 03 medical and health sciences, chemistry.chemical_compound, proteomics, Diethylhexyl Phthalate, neurone, comportement sexuel, Animals, Science Selection, préférence olfactive, ComputingMilieux_MISCELLANEOUS, mouse, media_common, phthalates, Behavior, Animal, Courtship display, Research, Public Health, Environmental and Occupational Health, Phthalate, standing reflex, follicle development, phtalate, neuron, [SDV.BIO] Life Sciences [q-bio]/Biotechnology, Mice, Inbred C57BL, ovarian follicle, 030104 developmental biology, chemistry, Reproduction, Autre (Sciences du Vivant)
Abstract

Background: Courtship behavior plays a critical role in attracting females and reproduction success. However, the effects of exposure to a ubiquitous contaminant di(2-ethylhexyl) phthalate (DEHP) on these behaviors and, in particular, on courtship vocalizations have not been examined. Objective: The effects of adult exposure to DEHP on courtship and mating behaviors and gonadotropic axis and neural mechanisms involved in DEHP-induced effects were analyzed in male mice. Methods: Adult C57BL/6J males were orally exposed to DEHP (0, 0.5, 5, and 50μg/kg/d) for 4 wk. Olfactory preference, ultrasonic vocalizations (USVs), partner preference and mating, as well as locomotor activity and motor coordination, were measured. The kisspeptin system and testosterone levels were analyzed. Proteomic and molecular studies were conducted on the hypothalamic preoptic nucleus, the key region involved in sexual motivation to vocalize and mate. Results: DEHP at 50μg/kg/d reduced the emission of USVs, whereas lower doses changed the ratio of syllable categories. This was associated with diminished sexual interest of female partners toward males exposed to 5 or 50μg/kg/d and increased latency to mate, despite normal olfactory preference. The kisspeptin system and circulating testosterone levels were unaffected. In DEHP-exposed males, proteomic analysis of the preoptic nucleus identified differentially expressed proteins connected to the androgen receptor (AR). Indeed, exposure to 5 or 50μg/kg/d of DEHP induced selective AR downregulation in this nucleus and upstream chemosensory regions. The involvement of AR changes in the observed alterations was further supported by the reduced emission of courtship vocalizations in males with disrupted neural AR expression. Conclusions: These data demonstrate the critical role of neural AR in courtship vocalizations and raises the possibility that the vulnerability of this signaling pathway to exposure to endocrine disrupters may be detrimental for courtship communication and mating in several species. https://doi.org/10.1289/EHP1443

Published
2017
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