1. Generation of mice with a conditional and reporter allele for Tmem100
- Author
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Young Jae Lee, Eun Hye Moon, S. Paul Oh, Yoo Sung Kim, Keum Soun Ko, Jiyoung Seo, and Mi-Jung Kim
- Subjects
Angiogenesis ,Activin Receptors, Type II ,Molecular Sequence Data ,Mice, Transgenic ,Biology ,Validation Studies as Topic ,Models, Biological ,Mice ,Endocrinology ,In vivo ,Genes, Reporter ,Conditional gene knockout ,Genetics ,Coding region ,Animals ,Amino Acid Sequence ,Transgenes ,Allele ,Alleles ,Cells, Cultured ,Sequence Homology, Amino Acid ,Kinase ,Gene Transfer Techniques ,Endothelial Cells ,Membrane Proteins ,ACVRL1 ,Cell Biology ,Embryo, Mammalian ,Molecular biology ,Transmembrane protein ,Organ Specificity ,Activin Receptors, Type I - Abstract
Activin receptor-like kinase 1 (ACVRL1; ALK1) is predominantly expressed in arterial endothelial cells and plays an important role in angiogenesis. ACVRL1 mutations cause hereditary hemorrhagic telangiectasia (HHT), a genetic vascular disorder for which the underlying mechanism is poorly understood. We have found that expression of transmembrane protein 100 (Tmem100) is downregulated in the lung of Acvrl1-deficient mice; however, its function is unknown. To elucidate the role of Tmem100 in vivo, we generated a conditional knockout allele for Tmem100 in which exon3, containing the entire coding sequence, was flanked by loxP sequences. The targeted allele also possessed a lacZ reporter cassette in intron2 for visualization of Tmem100 expression. We found that Tmem100 was predominantly expressed in arterial endothelial cells of developing embryos. The conditional and reporter allele will be a useful resource to investigate the in vivo role of Tmem100, especially in angiogenesis. genesis 48:673–378, 2010. © 2010 Wiley-Liss, Inc.
- Published
- 2010