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14 results on '"Ketscher, Lars"'

3. Cited4 is a sex‐biased mediator of the antidiabetic glitazone response in adipocyte progenitors

Author

Bayindir‐Buchhalter, Irem, Wolff, Gretchen, Lerch, Sarah, Sijmonsma, Tjeerd, Schuster, Maximilian, Gronych, Jan, Billeter, Adrian T, Babaei, Rohollah, Krunic, Damir, Ketscher, Lars, Spielmann, Nadine, Hrabe de Angelis, Martin, Ruas, Jorge L, Müller‐Stich, Beat P, Heikenwalder, Mathias, Lichter, Peter, Herzig, Stephan, and Vegiopoulos, Alexandros

Published
2018
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4. USP18 lack in microglia causes destructive interferonopathy of the mouse brain

Author

Goldmann, Tobias, Zeller, Nicolas, Raasch, Jenni, Kierdorf, Katrin, Frenzel, Kathrin, Ketscher, Lars, Basters, Anja, Staszewski, Ori, Brendecke, Stefanie M, Spiess, Alena, Tay, Tuan Leng, Kreutz, Clemens, Timmer, Jens, Mancini, Grazia MS, Blank, Thomas, Fritz, Günter, Biber, Knut, Lang, Roland, Malo, Danielle, Merkler, Doron, Heikenwälder, Mathias, Knobeloch, Klaus‐Peter, and Prinz, Marco

Published
2015
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7. High yield expression of catalytically active USP18 (UBP43) using a Trigger Factor fusion system

Author

Basters Anja, Ketscher Lars, Deuerling Elke, Arkona Christoph, Rademann Jörg, Knobeloch Klaus-Peter, and Fritz Günter

Subjects
Biotechnology, TP248.13-248.65
Abstract

Abstract Background Covalent linkage of the ubiquitin-like protein ISG15 interferes with viral infection and USP18 is the major protease which specifically removes ISG15 from target proteins. Thus, boosting ISG15 modification by protease inhibition of USP18 might represent a new strategy to interfere with viral replication. However, so far no heterologous expression system was available to yield sufficient amounts of catalytically active protein for high-throughput based inhibitor screens. Results High-level heterologous expression of USP18 was achieved by applying a chaperone-based fusion system in E. coli. Pure protein was obtained in a single-step on IMAC via a His6-tag. The USP18 fusion protein exhibited enzymatic activity towards cell derived ISG15 conjugated substrates and efficiently hydrolyzed ISG15-AMC. Specificity towards ISG15 was shown by covalent adduct formation with ISG15 vinyl sulfone but not with ubiquitin vinyl sulfone. Conclusion The results presented here show that a chaperone fusion system can provide high yields of proteins that are difficult to express. The USP18 protein obtained here is suited to setup high-throughput small molecule inhibitor screens and forms the basis for detailed biochemical and structural characterization.

Published
2012
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9. USP 18 lack in microglia causes destructive interferonopathy of the mouse brain

Author

Goldmann, Tobias, primary, Zeller, Nicolas, additional, Raasch, Jenni, additional, Kierdorf, Katrin, additional, Frenzel, Kathrin, additional, Ketscher, Lars, additional, Basters, Anja, additional, Staszewski, Ori, additional, Brendecke, Stefanie M, additional, Spiess, Alena, additional, Tay, Tuan Leng, additional, Kreutz, Clemens, additional, Timmer, Jens, additional, Mancini, Grazia MS, additional, Blank, Thomas, additional, Fritz, Günter, additional, Biber, Knut, additional, Lang, Roland, additional, Malo, Danielle, additional, Merkler, Doron, additional, Heikenwälder, Mathias, additional, Knobeloch, Klaus‐Peter, additional, and Prinz, Marco, additional

Published
2015
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10. Ubiquitin-Like Protein ISG15 (Interferon-Stimulated Gene of 15 kDa) in Host Defense Against Heart Failure in a Mouse Model of Virus-Induced Cardiomyopathy

Author

Rahnefeld, Anna, primary, Klingel, Karin, additional, Schuermann, Anett, additional, Diny, Nicola L., additional, Althof, Nadine, additional, Lindner, Anika, additional, Bleienheuft, Philipp, additional, Savvatis, Konstantinos, additional, Respondek, Dorota, additional, Opitz, Elisa, additional, Ketscher, Lars, additional, Sauter, Martina, additional, Seifert, Ulrike, additional, Tschöpe, Carsten, additional, Poller, Wolfgang, additional, Knobeloch, Klaus-Peter, additional, and Voigt, Antje, additional

Published
2014
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