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Your search keyword '"Kessler, Floortje L."' showing total 24 results
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24 results on '"Kessler, Floortje L."'

6. Exocytosis of polyubiquitinated proteins in bortezomib-resistant leukemia cells: a role for MARCKS in acquired resistance to proteasome inhibitors

Author

Franke, Niels E., primary, Kaspers, Gertjan L., additional, Assaraf, Yehuda G., additional, van Meerloo, Johan, additional, Niewerth, Denise, additional, Kessler, Floortje L., additional, Poddighe, Pino J., additional, Kole, Jeroen, additional, Smeets, Serge J., additional, Ylstra, Bauke, additional, Bi, Chonglei, additional, Chng, Wee Joo, additional, Horton, Terzah M., additional, Menezes, Rene X., additional, Musters, Renée J.P., additional, Zweegman, Sonja, additional, Jansen, Gerrit, additional, and Cloos, Jacqueline, additional

Published
2016
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7. Exosomes Secreted by Apoptosis-Resistant Acute Myeloid Leukemia (AML) Blasts Harbor Regulatory Network Proteins Potentially Involved in Antagonism of Apoptosis

Author

Wojtuszkiewicz, Anna, primary, Schuurhuis, Gerrit J., additional, Kessler, Floortje L., additional, Piersma, Sander R., additional, Knol, Jaco C., additional, Pham, Thang V., additional, Jansen, Gerrit, additional, Musters, René J.P., additional, van Meerloo, Johan, additional, Assaraf, Yehuda G., additional, Kaspers, Gertjan J.L., additional, Zweegman, Sonja, additional, Cloos, Jacqueline, additional, and Jimenez, Connie R., additional

Published
2016
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8. Abstract 4764: Transfer of regulatory protein networks via extracellular vesicles as a candidate mechanism of apoptosis-resistance in acute myeloid leukemia

Author

Wojtuszkiewicz, Anna, primary, Cloos, Jacqueline, additional, Kessler, Floortje L., additional, Piersma, Sander, additional, Knol, Jako, additional, Jansen, Gerrit, additional, Assaraf, Yehuda G., additional, Kaspers, Gertjan L., additional, Zweegman, Sonja, additional, Schuurhuis, Gerrit J., additional, and Jimenez, Connie R., additional

Published
2014
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9. Homing and clonogenic outgrowth of CD34+ peripheral blood stem cells: A role for L-selectin?

Author

De Boer, Fransien, Kessler, Floortje L., Netelenbos, Tanja, Zweegman, Sonja, Huijgens, Peter C., Van der Wall, Elsken, Van der Linden, Jan A.M., Pinedo, Herbert M., Schuurhuis, Gerrit Jan, Dräger, Angelika M., Hematology, CCA - Cancer biology and immunology, CCA - Imaging and biomarkers, CCA - Cancer Treatment and quality of life, Medical oncology, and Hematology laboratory

Abstract

Objective. After transplantation of hematopoietic stem cells, adhesion molecules play a major role in the multistep process of engraftment in which L-selectin is suggested to be of relevance. A positive correlation previously was found between the number of reinfused L-selectin+ stem cells and platelet recovery. In the present study, we determined the role of L-selectin in different engraftment steps, i.e., adhesion to endothelial cells, migration, and clonogenic outgrowth by in vitro assays that closely mimic the in vivo situation. Materials and Methods. Flow adhesion and migration experiments were performed using the human bone marrow endothelial cell line 4LHBMEC and isolated peripheral CD34+ cells with or without blocking of L-selectin-ligand interaction. Various clonogenic assays, including serum-free colony-forming unit-megakaryocytes (CFU-MK) and burst-forming unit-megakaryocytes (BFU-MK), were performed with sorted L-selectin+L-selectin- cells or in the presence of antibodies. Results. Blocking of L-selectin on CD34+ cells did not significantly affect rolling over and firm adhesion to 4LHBMEC. In addition, no role for L-selectin was found in transendothelial migration experiments. Finally, in clonogenic outgrowth of sorted or anti-L-selectin monoclonal antibody-incubated CD34+ cells, no key role for L-selectin expression could be defined in BFU-MK and CFU-MK assays. Conclusion. Using in vitro assays for CD34+ stem cell adhesion, migration, and clonogenic capacity, we were not able to define a major role for L-selectin. © 2002 International Society for Experimental Hematology. Published by Elsevier Science Inc.

Published
2002
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13. Subendothelial Heparan Sulfate Proteoglycans Become Major L-Selectin and Monocyte Chemoattractant Protein-1 Ligands upon Renal Ischemia/Reperfusion

Author

Celie, Johanna W.A.M., primary, Rutjes, Niels W.P., additional, Keuning, Eelco D., additional, Soininen, Raija, additional, Heljasvaara, Ritva, additional, Pihlajaniemi, Taina, additional, Dräger, Angelika M., additional, Zweegman, Sonja, additional, Kessler, Floortje L., additional, Beelen, Robert H.J., additional, Florquin, Sandrine, additional, Aten, Jan, additional, and van den Born, Jacob, additional

Published
2007
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18. Early Apoptosis Largely Accounts for Functional Impairment of CD34+Cells in Frozen-Thawed Stem Cell Grafts

Author

de Boer, Fransien, primary, Dräger, Angelika M., additional, Pinedo, Herbert M., additional, Kessler, Floortje L., additional, Monnee-van Muijen, M., additional, Weijers, Geert, additional, Westra, Guus, additional, van der Wall, Elsken, additional, Netelenbos, Tanja, additional, Oberink, Jan W., additional, Huijgens, Peter C., additional, and Schuurhuis, Gerrit J., additional

Published
2002
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22. Homing and clonogenic outgrowth of CD34+ peripheral blood stem cells: A role for L-selectin?

Author

de Boer, Fransien, Kessler, Floortje L., Netelenbos, Tanja, Zweegman, Sonja, Huijgens, Peter C., van der Wall, Elsken, van der Linden, Jan A.M., Pinedo, Herbert M., Schuurhuis, Gerrit Jan, and Dräger, Angelika M.

Subjects
*HEMATOPOIETIC stem cells, *CELL adhesion molecules
Abstract

: ObjectiveAfter transplantation of hematopoietic stem cells, adhesion molecules play a major role in the multistep process of engraftment in which L-selectin is suggested to be of relevance. A positive correlation previously was found between the number of reinfused L-selectin+ stem cells and platelet recovery. In the present study, we determined the role of L-selectin in different engraftment steps, i.e., adhesion to endothelial cells, migration, and clonogenic outgrowth by in vitro assays that closely mimic the in vivo situation.: Materials and MethodsFlow adhesion and migration experiments were performed using the human bone marrow endothelial cell line 4LHBMEC and isolated peripheral CD34+ cells with or without blocking of L-selectin–ligand interaction. Various clonogenic assays, including serum-free colony-forming unit-megakaryocytes (CFU-MK) and burst-forming unit-megakaryocytes (BFU-MK), were performed with sorted L-selectin+L-selectin− cells or in the presence of antibodies.: ResultsBlocking of L-selectin on CD34+ cells did not significantly affect rolling over and firm adhesion to 4LHBMEC. In addition, no role for L-selectin was found in transendothelial migration experiments. Finally, in clonogenic outgrowth of sorted or anti–L-selectin monoclonal antibody-incubated CD34+ cells, no key role for L-selectin expression could be defined in BFU-MK and CFU-MK assays.: ConclusionUsing in vitro assays for CD34+ stem cell adhesion, migration, and clonogenic capacity, we were not able to define a major role for L-selectin. [Copyright &y& Elsevier]

Published
2002
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23. Early Apoptosis Largely Accounts for Functional Impairment of CD34+ Cells in Frozen-Thawed Stem Cell Grafts

Author

de Boer, Fransien, Dräger, Angelika M., Pinedo, Herbert M., Kessler, Floortje L., Monnee-van Muijen, M., Weijers, Geert, Westra, Guus, van der Wall, Elsken, Netelenbos, Tanja, Oberink, Jan W., Huijgens, Peter C., and Schuurhuis, Gerrit J.

Abstract

Quality assessment of stem cell grafts is usually performed by flow cytometric CD34+ enumeration or assessment of clonogenic output of fresh material. Previously, we identified the occurrence of early apoptosis, not detectable with the permeability marker 7-amino actinomycin D (7-AAD), in purified frozen-thawed CD34+ cells, using the vital stain Syto16. Sytohigh/7-AAD- cells were defined as viable, Syto16low/7-AAD- cells as early apoptotic and Syto16low/7-AAD+ as dead. This was confirmed in a subsequent study using frozen-thawed transplants of lymphoma patients. In the present study on grafts from multiple myeloma and lymphoma patients, we investigated the functional consequences of the early apoptotic process. The mean Syto16-defined viability was 41 and 42%, respectively, for both graft groups, compared to 78% and 72%, respectively, using 7-AAD only. The established early apoptosis marker annexin V missed roughly 50% of the early apoptosis detected with Syto16. In contrast, viability of CD34+ cells in nonmanipulated whole blood transplants from a matched group of lymphoma patients, after 72 h of storage at 4°C, was more than 90%, even with the Syto16 assay. CFU recovery (median 26-33%) after cryopreservation matched CD34+ recovery after Syto16, but not 7-AAD correction. In contrast, colony-forming unit (CFU) recovery in the whole blood transplant was close to 100%. Furthermore, early apoptotic CD34+ cells had lost migratory ability toward stromal cell derived factor-1α (SDF-1α). The establishment of a Syto16high/7-AAD- proportion of CD34+ cells offers a new approach for a more correct determination of the number of viable nonapoptotic CD34+ cells in stem cell grafts. Further development of this assay should allow its incorporation into the routine CD34+ assessment of post-thawed samples in clinical flow cytometry laboratories.

Published
2002
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24. Conditioning Regimens in Stem Cell Transplantations Facilitate Homing by Increasing Bone Marrow SDF-1 through Induction of SDF-1Gene Transcription.

Author

Zweegman, Sonja, Janssen, Jeroen J.W.M., Kessler, Floortje L., Mus, Adriana M.C., van den Born, Jacob, Huijgens, Peter C., and Drager, Angelika M.

Abstract

Stromal Derived Factor-1 (SDF-1) is a key regulator of engraftment. The clinical relevance of SDF-1 is suggested by the relation between in vitro migratory capacity of CD34-positive cells towards SDF-1 and engraftment after stem cell transplantation (SCT). For better insight in engraftment after SCT clarifying the determinants of SDF-1 is therefore important. We investigated SDF-1 responses after chemotherapy and irradiation of the murine stromal M210B4 cell line and in allogeneic SCT patients. SDF-1 protein levels were measured in conditioned medium (CM) of the M210B4 cell line, in peripheral blood plasma and bone marrow plasma by ELISA. The migration supportive capacity of CM was tested by allowing CD34-positive cells to migrate over fibronectin coated transwells for 4 hours. Furthermore, sdf-1 gene expression was investigated by means of a quantitative PCR using mRNA from M210B4 cells or from buffy coats of peripheral blood and bone marrow cells.

Published
2004
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