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3 results on '"Kerche-Silva Le"'

1. Cystic Fibrosis Late Diagnosis: A Case Report

Author

Kerche-Silva Le, Carolino Mlo, de Moraes Junior Rm, Mota Gs, and Marques Junior Os

Subjects
Pediatrics, medicine.medical_specialty, Bronchiectasis, business.industry, food and beverages, Disease, medicine.disease, Cystic fibrosis, Mucus, SWEAT, Late diagnosis, Recurrent pneumonia, medicine, business
Abstract

Cystic Fibrosis (CF) is an autosomal recessive disease that affects mucus and sweat producing cells involving multiple organs. CF is usually diagnosed in childhood; however, a considered number of adults are diagnosed every year. Atypical CF can be a milder form of the CF disorder, and individuals with atypical CF can remain undiagnosed for many years. Physicians should suspect of CF in adult individuals when these present recurrent pneumonia or bronchiectasis. Therefore, since early diagnosis of CF can avoid morbidities and unnecessary hospitalizations, the recognition of CF symptoms by clinicians is necessary to avoid late diagnosis.

Published
2017
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2. In vitro cytotoxicity and genotoxicity of composite mixtures of natural rubber and leather residues used for textile applications.

Author

Cavalcante DG, Gomes AS, Dos Reis EA, Danna CS, Kerche-Silva LE, Yoshihara E, and Job AE

Subjects
Animals, CHO Cells, Cell Line, Clothing, Comet Assay, Cricetulus, Humans, Industrial Waste adverse effects, Industrial Waste analysis, Chromium analysis, DNA Damage drug effects, Rubber toxicity, Textiles toxicity
Abstract

A novel composite material has been developed from natural rubber and leather waste, and a corresponding patent has been filed. This new material may be incorporated into textile and footwear products. However, as leather waste contains chromium, the biocompatibility of this new material and its safety for use in humans must be investigated. The aim of the present study was to investigate the presence of chromium in this new material, determine the amount of each form of chromium present (trivalent or hexavalent), and evaluate the potential cytotoxic and genotoxic effects of the novel composite in two cell lines. The cellular viability was quantified using the MTT3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide reduction method and neutral red uptake assay, and genotoxic damage was analyzed using the comet assay. Our findings indicated that the extracts obtained from the composite were severely cytotoxic to both cell lines tested, and additionally highly genotoxic to MRC-5 cells. These biological responses do not appear to be attributable to the presence of chromium, as the trivalent form was predominantly found to be present in the extracts, indicating that hexavalent chromium is not formed during the production of the novel composite. The incorporation of this new material in applications that do not involve direct contact with the human skin is thus indicated, and it is suggested that the chain of production of this material be studied in order to improve its biocompatibility so that it may safely be used in the textile and footwear industries.

Published
2017
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3. In vitro protective effects of botryosphaeran, a (1→3;1→6)-β-d-glucan, against mutagens in normal and tumor rodent cells.

Author

Kerche-Silva LE, Cólus IM, Malini M, Mori MP, Dekker RF, and Barbosa-Dekker AM

Subjects
Animals, Cricetinae, Cricetulus, In Vitro Techniques, Mutagenicity Tests, Rats, Antimutagenic Agents pharmacology, Glucans pharmacology
Abstract

Botryosphaeran (BOT) is an exocellular β-d-glucan (carbohydrate biopolymer) of the (1→3;1→6)-linked type produced by Botryosphaeria rhodina MAMB-05. The cytotoxic, mutagenic, genotoxic, and protective effects of this substance were evaluated in Chinese hamster lung fibroblasts (V79) and rat hepatocarcinoma cells (HTC) by the micronucleus test (MN) and the comet assay. BOT was not genotoxic in either cell line; it decreased the clastogenic effects of doxorubicin, H 2 O 2 , and benzo[a]pyrene. These results indicate that BOT may have potential as a therapeutic agent., (Copyright © 2016 Elsevier B.V. All rights reserved.)

Published
2017
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