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Fescue toxicosis has a negative impact on livestock production. Tannins could bind to tall fescue toxins rendering them ineffective. The objective was to improve health and well-being of sheep offered tall fescue infected with the endophytic fungus (Neotyphodium coenophialum) by binding toxins in the rumen with condensed tannins. Non-pregnant, non-lactating ewes (n=20; 57±1.3 kg initial BW) were housed in individual 1×1.5-m pens with grate flooring and were offered ad libitum access to either non-toxic novel-endophyte infected tall fescue haylage (NE), or toxic endophyte infected tall fescue haylage with no condensed tannin (E0), or condensed tannin at 10 (E10), or 30 g/kg haylage DM (E30). Additionally, ewes received 4 g/kg BW liquid molasses, which served as the carrier for the condensed tannins in the E10 and E30 treatments. The study consisted of 2, 21-d periods with a 30-d wash out between periods. In each period, a 14-d adaption was followed by 7 d of total fecal and urine collection. Temperature was maintained at 27 to 29 °C. Dry matter and OM intake (g/kg BW; DMI and OMI, respectively) were not affected (P ≥ 0.15) by treatment, but DM and OM digestibility were greater (P < 0.05) from NE vs E30. Digestibility of NDF was greater (P < 0.05) from NE vs E0, E10, and E30. Digestible DMI was greater (P < 0.05) from NE and E0 vs E30, and digestible OMI was greater (P < 0.05) from NE vs E30. Apparent N absorbed (g/kg N intake) was greater (P < 0.05) by lambs offered E0 and E10 compared with E30. Nitrogen retained (g/kg N intake and N absorbed) did not differ (P≥0.29). Therefore, overall impacts of ensiled toxic fescue on lambs were inconsistent and the impact of adding condensed tannins at 30 g/kg DM was negative on most of the DM and OM digestibility measurements.

Forage management and environmental conditions affect water soluble carbohydrate (WSC) storage, and, in turn, influence ruminant forage utilization in silvopastoral systems. The objective was to determine effects of four dependent variables: forage species [(non-native, C3 (orchardgrass (Dactylis glomerata L.)) and native C4 mix (8:1:1 big bluestem (Andropogon gerardii Vitman), little bluestem (Schizachyrium scoparium Michx. Nash) and indiangrass (Sorghastrum nutans L.))], fertility (poultry litter and an unfertilized control), forage sampling date (mid-May, late-May, early-June, mid-June, and late-June), and hour of day (0800, 1100, 1400, and 1700 h) on WSC accumulation in a silvopasture. Concentrations of WSC (g kg DM−1) were greater (p ≤ 0.05) for C3 forages, with poultry litter not impacting WSC accumulation. Overall, WSC was greatest in mid-June, with the lowest WSC concentration observed at 0800 compared to 1100, 1400, and 1700 h (p ≤ 0.05). Therefore, harvesting forages later in the day resulted in greater WSC. A stepwise regression model indicated acid detergent fiber, ash, and forage P concentration were the best predictors (R2 = 0.85, p ≤ 0.05) of forage WSC. These results may be useful in future studies aimed at explaining diurnal cattle grazing preference and optimum forage harvest timing in silvopastoral systems.

Seventeen crossbred lambs were assigned randomly to low-protein (LP; 8% crude protein [CP]; n = 9) and high-protein (HP; 13% CP; n = 8) diets for 9 weeks. The final body weight, average daily feed intake (ADFI), and average daily gain (ADG) of the HP lambs were significantly higher (P

Tall fescue (Schedonorus arundinaceus) is a cool-season perennial grass that is widely used as a forage for many livestock species including sheep. An endophyte (Neotyphodium coenophialum) in tall fescue produces ergot alkaloids that enhance plant survival but produce toxicosis in animals. The objective of this study was to investigate the rumen microbiota from gestation and lactation in ewes grazing tall fescue pastures with high (HA) or moderate (MA) levels of endophyte infection, and their relationship with serum parameters. Data were collected at the beginning of the study (d1), the week before initiation of lambing (d51), and at the end of the trial (d115). The rumen microbiota was evaluated using 16S rRNA gene sequencing. Ewes grazing HA had greater serum non-esterified fatty acid (NEFA) (P = 0.024) levels compared with ewes in MA pasture at d115. Both the number of observed OTUs and Shannon diversity index tended (P = 0.08, P = 0.06) to be greater for HA than for MA on d115. At the genus level, Prevotella relative abundance increased with time in both MA and HA (on d1, d51 and d115: 15.17%, 25.59% and 24.78% in MA; 14.17%, 18.10% and 19.41% in HA). Taxa unclassified at the genus level including (unclassified) Lachnospiraceae, Coriobacteriaceae and Veillonellaceae exhibited higher abundances in HA at d51 (3.72%, 2.07% and 11.22%) compared with MA (2.06%, 1.28% and 7.42%). The predictor microbiota for HA and MA were identified by a random forest classification model. The HA predictors included bacteria associated with unclassified Coriobacteriaceae and Ruminococcaceae. Other OTUs classified as Prevotella and Clostridiales could be microbial predictors for MA. The OTUs classified as Prevotella and Lachnospiraceae were negatively correlated with serum concentration of prolactin. Negative correlations with NEFA were observed in the microbiota such as species affiliated to unclassified Clostridiales and Prevotella. OTUs classified as Bacteroidetes and Coriobacteriaceae exhibited a positive correlation with NEFA. Our study confirmed that the rumen microbiota populations were affected by high levels of toxins in endophyte-infected tall fescue and were associated with host hormone and energy metabolism.

Objective was to examine the activity of cytochrome P450 (CYP), a liver enzyme that metabolizes ergot alkaloids which is hindered by this fescue toxin, as an indicator of fescue toxicosis in sheep. In spring and fall, ewes were blocked by age and BW, and randomly assigned to 1 kg/ewe/d of endophyte-infected (E+; 61% of diet in fall, 13% of diet in spring; 0.8 µg/g of ergovaline; and soy hulls, alfalfa pellets, cottonseed hulls, molasses) or non-infected (E-; same proportion of E+/season and same feeds) tall fescue seed supplement (n = 10/diet in each season) for 28 d, plus hay, mineral and water. Ewes were exposed to teaser, d -21 to 0, and intact rams, d 0 (first day of diet) to 30. Rectal temperature (RT), serum concentration of prolactin (using RIA), and CYP were determined every 7–14 d between d 0–28, and pregnancy status on d 56. PromegaTM P450-Glo assay was used to determine CYP from serum. Data were analyzed by using PROC MIXED with repeated measures (SAS). Rectal temperature of E+ compared with E- ewes was elevated on at least one day in fall and spring (diet × season × d, P < 0.001). Serum prolactin was lower in E+ compared with E- ewes (diet × d, P < 0.001) and lower in fall (P < 0.001). CYP was higher in E- ewes on d 28 in fall (diet × season × d, P = 0.008), but otherwise similar between diets, and higher in fall than spring (P < 0.001). There was an unexpected negative correlation between CYP and prolactin (R = -0.24; P < 0.009), but there appears to be a subpopulation of ewes with low prolactin and lower CYP. CYP activity in sera may not be a marker for fescue toxicosis in ewes.

Forage for grazing and hay may be limited during drought years, necessitating alternative feeding options. Co-product feedstuffs, such as soybean hulls (SH), may be a more economical means to maintain cows through such conditions. The objective of this study was to determine if SH could be used to meet the majority of the energy demands for cows in late gestation when access to hay was restricted to 1 h daily. Eighty-six gestating, spring-calving cows (527 ± 7.5 kg initial BW; 4.3 ± 0.27 yr of age) were allocated to 1 of 6 groups for a 68-d study beginning December 6, 2012. Three groups were given ad libitum access to medium-quality bermudagrass [Cynodon dactylon (L.) Pers.] hay. The 3 remaining groups were offered 6.4 kg of SH/cow daily and allowed access to a very-poor-quality, mature, mixed-grass hay for only 1 h daily. Each group was housed in separate 2.02-ha dormant bermudagrass pastures. Birth weight and dystocia scores were recorded, and calf performance was monitored through weaning. Representative bales of each hay were weighed to determine total hay offered. Differences in BW and BCS, and changes in these measurements during the study, were minimal between treatments (P ≥ 0.53). Calf birth weight, weaning weight, BW/d of age at weaning, and birth-to-weaning ADG did not differ (P ≥ 0.11) between treatments. Therefore, SH may be used as the major dietary energy component for cows in late gestation having access to hay for only 1 h daily without having adverse effects on the cows or their subsequent calves.

Moisture concentration and time between baling and wrapping round bale silage impacts silage quality and aerobic stability. The objective of this study was to observe the effects of wrapping delays of 0, 1, 2, or 3 days on the nutritional quality and storage characteristics of annual ryegrass (Lolium multiflorum) silage baled at two levels of moisture (30 ± 9.5% [DRY] or 74 ± 0.8% [WET]). The DRY (n = 18) and WET (n = 20) bales were assigned as replicates and wrapped by pasture quadrant, then stored for 85 d. Crude protein, ADF, NDF, and ADICP of silage were greater (P < 0.01) for DRY than WET. Wrapping delays did not change bale weight or CP , but DM concentrations decreased linearly (P = 0.03) and ADF, NDF, and ADICP increased (P = 0.02) linearly with wrapping delay. Maximum temperature of DRY was greater (P = 0.01) than WET and increased linearly with wrapping delays (P < 0.01). Changes in the fermentation profile of WET bales with delays in wrapping indicated clostridial activity occurred, which will likely result in reductions in voluntary intake by livestock. Based on these results, ryegrass fermentation patterns and nutritional quality show a decline with each day delayed before wrapping with WET silage.

Certain enzymes may enhance fiber digestion, but the optimum dosage for ruminants consuming bermudagrass hay is not known. The objective of this study was to determine the optimal dosage of a combination of a protease with Aspergillus oryzae and A. niger fermentation extract blend on fiber digestion and rumen fermentation of bermudagrass hay by sheep. Twenty ewes (30.3 ± 5.29 kg BW) were allocated randomly to 1 of 5 diets (n = 4/treatment). Bermudagrass hay was chopped and offered for ad libitum consumption to ewes housed individually in 1 × 1.5-m pens with plastic-coated grate flooring. Diets were supplemented with 0.25% BW of soybean meal and either no enzyme or 1.5, 3, 4.5, or 6 g of a mixture of a protease with A. oryzae and A. niger fermentation extracts in a calcium carbonate carrier. A 14-d dietary adaptation was followed by 7 d of total fecal collection. On d 21, 3 rumen samples per ewe were taken at 4 h intervals for analysis of pH and VFA concentrations. Data were analyzed using the MIXED procedure of SAS and orthogonal linear and quadratic contrasts were used to assess enzyme dosage effects. Dry matter and ADF digestibility increased quadratically (P < 0.05), and NDF and OM digestibility tended (P ≤ 0.07) to increase quadratically across dosages. Ruminal acetate concentrations decreased quadratically and ruminal propionate increased quadratically (P < 0.05) with increasing enzyme dosage (P < 0.05). Total VFA concentrations were not affected by dosage (P = 0.71), but the acetate:propionate ratio decreased quadratically (P < 0.05) with increasing enzyme dosage. In conclusion, a protease and A. oryzae and A. niger fermentation extract blend increased fiber digestion, but dosages over 3 g of enzyme did not further enhance DMD or DMI.

Certain enzymes have improved fiber digestion of low-quality forages. Our objective was to determine the efficacy of a combination of a protease and Aspergillus oryzae and A. niger fermentation extracts on fiber digestion and rumen fermentation of bermudagrass hay supplemented with different energy concentrates. Bermudagrass hay was chopped and offered for ad libitum consumption to 20 ewes per study (33.1 ± 10.65 kg BW; Exp. 1; 36.0 ± 11.10 kg BW; Exp. 2) housed individually in 1 × 1.5-m pens (n = 5/trt). In Exp. 1, hay supplemented with DDGS at 0.25% BW, 1% BW, 1% BW + 2.3 g enzyme, or 1% BW + 3.5 g enzyme. In Exp. 2, hay was supplemented with cracked corn at the same levels as used in Exp. 1. Each experiment consisted of a 14-d dietary adaptation followed by 7 d of total fecal collection. On d 21, rumen samples were taken at 3 h intervals from each ewe for analysis of pH and VFA concentrations. Data were analyzed using PROC MIXED of SAS and orthogonal contrasts compared 0.25% vs. 1% BW, no enzyme vs. 2.3 g/d enzyme and 2.3 g/d vs. 3.5 g/d enzyme. In Exp. 1 NDF digestibility was greater (P < 0.05) from 1% vs. 0.25% BW supplement. Ruminal acetate and the acetate:propionate ratio were greatest (P < 0.05) from 0.25% BW vs. the other diets. Exp. 2, DM and OM intake were greater (P < 0.05) from the 1% BW diets and tended (P = 0.08) to be greater from 1% BW alone vs. 1% BW + 2.3 g enzyme. Ruminal acetate concentrations were greatest (P < 0.05) from 0.25% BW. Therefore, the blend of A. oryzae and A. niger fermentation extracts and a protease was not effective under the conditions of this study.

Exposing ensiling forage to oxygen can result in dry matter deterioration and reduce silage intake by animals. This study was conducted to investigate the effects of two different wrapping sources and time intervals between baling and wrapping on N utilization in gestating sheep offered alfalfa silage. Alfalfa silage was baled in large round bales (moisture concentration = 591 ± 43.0 g/kg), then wrapped with plastic using either an enhanced oxygen-limiting barrier (EOB) or no additional barrier (conventional wrap; CW) on the day of baling or 1, 2 or 3 d after baling. After 5 months of storage, silages were chopped, then offered randomly for ad libitum consumption to 16 gestating ewes [63.5 ± 1.71 kg body weight] to provide 2 observations per treatment for each of 3 experimental total collection periods. Each study period consisted of a 10-d dietary adaptation period followed by 7 d of total fecal and urine collection. Blood was collected immediately prior to feeding and four hours after feeding on the last day of each period. Data were analyzed using PROC MIXED of SAS for a 2 × 4 factorial treatment arrangement, while blood data were treated as repeated measures. When expressed as a portion of total N, neutral detergent insoluble N (NDIN) content of silage increased (P ≤ 0.02) linearly and quadratically, and acid detergent insoluble N (ADIN) concentrations increased (P

Bermudagrass ( L.) is a familiar forage in the equine industry and teff () is gaining popularity as well. However, it is unclear if the application of poultry litter as a fertilizer affects palatability of these forages in horses. Therefore, the objective of this study was to determine if forage species and timing of litter application as a fertilizer has an effect on preference by horses. Hay treatments were arranged in a 2 × 3 factorial treatment arrangement consisting of teff and bermudagrass harvested after no poultry litter application (NL), poultry litter applied to stubble immediately after removal of the previous cutting (L0), or poultry litter applied 14 d after the previous cutting (L14). Mature, stock-type geldings ( = 5; 480 ± 52.9 kg) were used in this study arranged as a balanced incomplete block design. Horses were offered different combinations of 4 of the 6 total forages daily for 3 d in each of 3 evaluation periods that immediately followed a 10-d adaptation period. Each forage was offered at half of the total daily DMI as measured during the last 5 d of the 10-d adaptation period to encourage selection among the 4 forages. Each hay offered was randomly allocated to a corner and suspended in hay nets over muck buckets in the corners of each stall. Horses were individually housed in 3.6- by 3.6-m indoor stalls with sand bedding and access to 3.6- by 7.6-m outdoor runs. Along with hay, horses were offered oats twice daily at 0.125% of BW at each feeding. Dry matter intake was greater ( < 0.01) for bermudagrass than for teff and for NL and L0 treatments compared with L14 treatments. Horses spent more ( < 0.01) time consuming bermudagrass compared with teff. However, there were no differences ( ≥ 0.25) in time spent consuming hay across litter treatments. Therefore, horses may prefer bermudagrass to teff and later application of poultry litter may affect voluntary intake by horses. However, all forages were mature, which may have impacted total intake and preference.

The production of baled silage is attractive to producers because it offers advantages over dry hay, particularly by limiting risks associated with wet or unstable weather conditions. Our objectives were to test the effects of delayed wrapping on silage fermentation, storage characteristics, and the nutritive value of baled alfalfa silages. To accomplish this, large-round bales of alfalfa were wrapped in plastic film within 4h of baling (d 0), or after delays of 1, 2, or 3 d. A secondary objective was to evaluate a prototype bale wrap containing an O2-limiting barrier (OB) against an identical polyethylene wrap without the O2 barrier (SUN). Sixty-four 1.19×1.25-m bales of alfalfa were made from 4 field blocks at a mean moisture concentration of 59.1±4.3% with a mean initial wet bale weight of 473±26.4kg. Two bales per field block were assigned to each combination of bale wrap (SUN or OB) and wrapping time (0, 1, 2, or 3 d postbaling), and one bale of each pair was fitted with a thermocouple placed in the geometric center of each bale. All bales were sampled after a 97-d storage period. Internal bale temperatures, recorded at the time bales were wrapped, were greater for all bales with wrapping delays compared with bales wrapped on d 0 (54.9 vs. 34.9°C), and increased to a maximum of 63.9°C after a 3-d delay exhibiting a linear effect of time delay. Total silage fermentation acids (lactic, acetic, propionic, butyric, and isobutyric) were greatest when bales were wrapped on d 0 compared with all bales wrapped with time delays (4.64 vs. 2.26% of DM), and declined with linear and quadratic effects of wrapping delay. Total fermentation acids also were related quadratically to internal bale temperature by regression [y (% of DM)=0.0042x(2) - 0.50x + 17.1; R(2)=0.725]. Similar responses were observed for lactic acid, except that trends were linear, both for orthogonal contrasts evaluating length of wrapping delay, and in regressions on internal bale temperature [y (% of DM)=-0.046x + 3.5; R(2)=0.663]. Butyric acid also was detected, regardless of treatment, but was greatest within bales wrapped on d 0 compared with those with wrapping delays (0.99 vs. 0.38% of DM), and a similar response (0.68 vs. 0.52% of DM) was observed for NH3-N, suggesting that clostridial activity occurred during silage fermentation. Based on these results, silage fermentation characteristics and the nutritive value declined with time delays before wrapping, but responses were exacerbated when delays exceeded 1 d.

(ProQuest: ... denotes formulae omitted.)Stakeholders in the cow-calf industry are under ongoing financial and regulatory pressure to revise production methods to improve their economic and environmental efficiency. Several studies estimated that cow-calf production practices commonly used in North America are the greatest source of greenhouse gas (GHG) emissions of all beef-cattle enterprises (Lupo et al. 2013,Beaucheminetal.2011, Pelletier, Pirog, and Rasmussen 2010). While methods for mitigating GHG emissions from cow-calf operations are needed (Beauchemin et al. 2011), such measures can reduce productivity and hence decrease profitability (Zilverberg et al. 2011). Furthermore, a combination of volatile input and commodity prices and increasing environmental regulation has created a need for tools by which producers can better evaluate various inputs and production methods when making decisions at the farm level. University of Arkansas created decision-support software, Forage and Cattle Planner (FORCAP), that producers, extension agents, and researchers can use to estimate net returns and changes in GHG emissions for different cow-calf and forage-management strategies and inputs (Popp et al. 2014). The FORCAP spreadsheet allows users to enter operation-specific parameters, inputs, and production methods to compare estimated net returns and GHG emissions in Excel®. The software and accompanying reference and user manuals are available online (Keeton et al. 2014, Smith, Popp, and Keeton 2013).In this analysis, we used FORCAP and an Excel® add-in, Risk Solver Platform v12.5 (Frontline Systems Inc.), to simulate profit-maximizing forage and cattle management decisions for medium and large size operations in a steady-state environment with no growth in herd size. The profit-maximizing model modified the species composition of pasture forage, the stocking rate, and weaning age for three calving distributions-spring, fall, and year-round- using three pasture-fertilization scenarios-none, low, and high-for each operation size, resulting in eighteen scenarios in all. This analysis examined the potential net returns and GHG emission tradeoffsbetweendifferent input selections and production decisions. For example, increasing pasture fertilization may be more profitable by allowing for a greater stocking rate but may lead to a higher environmental cost by way of both the increased number of animals and greater fertilizer emissions per acre. Conversely, it may be more profitable not to fertilize given the high cost of fertilizing, thus reducing GHG emissions from fertilizer and potentially reducing beef production and thereby animal emissions per acre. This analysis provides practical information about likely changes in beef production, profitability, and GHG emissions in response to modifications to pasture fertilization, calving distributions, pasture species composition, weaning age, and stocking rates.BackgroundChanges in the composition of pasture forage species can lead to seasonal forage growth that more closely matches the monthly nutritional requirements of the animals for a given calving season and weaning age. Further, inclusion of nitrogen-fixing species can reduce commercial nitrogen fertilizer requirements (West and Waller 2007) and hence reduce GHG emissions from upstream fertilizer production and nitrous oxide emissions from nitrogen that volatizes when such fertilizers are applied. However, inclusion of these nitrogen-fixing species may require additional production costs (Zilverberg et al. 2011).In Arkansas, pasture-forage species can be broadly categorized as warm-season grasses, cool-season grasses, and legumes. Warm-season grasses actively grow from late spring to late summer (May to August). Cool-season grasses typically grow from early spring to early summer (March to June) and are dormant during periods of high temperatures in mid-summer (July and August). These grasses typically resume growing in the fall (September to November) as temperatures decrease. …

Prolonged feeding of sericea lespedeza (SL) previously led to reduced serum concentrations of Mo, a cofactor in an enzyme complex that may be involved in weight gain. The current objective was to determine the effect of Mo supplementation on changes in serum, fecal, urine, and liver concentrations of trace minerals in lambs fed SL leaf meal pellets. Thirty ram lambs weaned in May (84 ± 1.5 d of age and 27 ± 1.1 kg; D 0) were blocked by BW, breed type (full or three-fourths Katahdin), and EBV of parasite resistance and randomly assigned to be fed 900 g/d of an alfalfa-based supplement (CON; = 10) or a SL-based supplement ( = 20) for 103 d. Supplements were formulated to be isonitrogenous and isocaloric and to meet trace mineral requirements. Within the SL group, individual lambs were administered either 5 mL water or 5 mL of water with 163.3 mg of sodium molybdate (SLMO). Serum was collected on d 28, 56, and 104; a liver sample was collected by biopsy on d 104 to determine concentrations of trace minerals. Data were analyzed using a mixed model and orthogonal contrasts. Serum concentrations of Mo increased in response to the drench and were greatest in SLMO lambs and then CON lambs and lowest in SL lambs ( < 0.001). Concentrations of Mo in the liver ( < 0.001) were similar between CON and SLMO lambs and were lower in SL lambs than other groups. Serum ( < 0.001) and liver ( = 0.013) concentrations of zinc (Zn) were reduced in both SL and SLMO lambs compared with CON lambs. Serum concentrations of cobalt (Co) increased in CON lambs compared with SL and SLMO lambs between d 0 and 56 but were similar on d 104 (diet × day, < 0.005) as with concentrations in the liver. Serum and liver concentrations of copper (Cu) were greatest ( < 0.001 and < 0.001, respectively) in CON lambs followed by SL lambs and then SLMO lambs. Serum concentrations of selenium (Se) tended ( = 0.10) to be reduced in SL lambs compared with CON and SLMO lambs, but concentrations in the liver were reduced in SL lambs compared with CON lambs and even more so in SLMO lambs ( < 0.003). Although the dietary Mo did increase stores of Mo in the animal and reduced copper, trace minerals associated with metalloproteins-Mo, copper, selenium, and zinc-were reduced in the liver of SL- and/or SLMO-fed lambs. These reductions could be associated with the lower weight gains previously observed after prolonged feeding of SL.

Dairy slurry is used commonly as an animal-sourced fertilizer in agronomic production. However, residual effects of slurry application on intake and digestibility of alfalfa (Medicago sativa L.) silage from subsequent harvests are not well known. The objective of this study was to determine if moisture concentration of alfalfa silage and timing of dairy slurry application relative to subsequent harvest affected intake and digestibility by sheep. Katahdin crossbred ewes (n = 18; 48 ± 5.3 kg) in mid-gestation were stratified by BW and allocated randomly in each of two periods to one of six treatments arranged in a two × three factorial arrangement. Treatments consisted of recommended (RM; 46.8%) or low (LM; 39.7%) moisture at baling after either no slurry application (NS), slurry application to stubble immediately after removal of the previous cutting (S0), or slurry application 14 d after removal of the previous cutting (S14). Silages were chopped through a commercial straw chopper, packed into plastic trash cans, and then offered to ewes within 4 d of chopping. Period 1 of the intake and digestion study consisted of a 14-d adaptation followed by a 7-d fecal collection period. Period 2 followed period 1 after a 4-d rest and consisted of an 11-d adaptation followed by 7 d of fecal collection. Ewes were housed individually in 1.4 × 4.3-m pens equipped with rubber mat flooring. Feces were swept from the floor twice daily, weighed, and dried at 50 °C. Ewes had ad libitum access to water and were offered chopped silage for a minimum of 10% refusal (DM). Blood samples were collected immediately prior to feeding, and 4 and 8 h after feeding on the day prior to the end of each period. Organic matter intake (g/kg BW) and OM digestibility tended (P 0.17) across treatments. Therefore, moisture concentration of alfalfa silage within the range used in this study may not affect voluntary intake or digestibility, but slurry application may have an effect on digestible OM intake. Also, moisture concentration of alfalfa silage and time of dairy slurry application may affect specific blood hemograms.

The objective of this study was to determine the effect of harvesting alfalfa and fescue after frost and ensiling mixtures of these forages on silage fermentation characteristics, intake, digestibility and ruminal fermentation by ewes. Forages were harvested in October of 2018, wilted, and blended as either alfalfa alone, 67% alfalfa +33% fescue, 33% alfalfa + 67% fescue, or 100% fescue. Twenty bins were lined with 2 plastic bags, packed (n = 5/trt), stored for 3 months, and then randomly assigned to ewes (n = 20; mean BW = 34.7 ± 6.65 kg) for ad libitum consumption, using 5 animals per treatment. Ewes were given 17 d of adaptation followed by 5 d of total feces and urine collection. Data were analyzed using PROC-MIXED of SAS and orthogonal polynomial trends were used to identify the effects of different proportions of fescue-to-alfalfa silage. Silage total acids, lactate, and acetate increased linearly (P < 0.01) while silage moisture and ammonia decreased linearly (P < 0.01) with increasing proportions of fescue in the diet. The proportions of lactate to total acids (mole/100 moles) increased linearly (P < 0.01) and quadratically (P < 0.05) by adding more fescue to alfalfa. Intake (g/kg BW), digestibility (%), and intake of digestible DM and OM (g/kg BW) decreased linearly (P < 0.05) as fescue proportion was increased in the silages. Ruminal acetate and butyrate (%) increased linearly (P < 0.01) with increasing fescue concentrations in the diet. Ensiling alfalfa with fescue may improve forage fermentation characteristics, but may reduce intake and digestibility in sheep compared to feeding alfalfa silage alone. This study was supported in part by USDA-ARS specific cooperative agreement 58-3655-4-052.

The objective of this paper is to present a systematic review and meta-analysis of research efforts aimed at recovering cattle production losses attributed to toxic endophyte-infected [ (Morgan-Jones & Gams.) Glenn, Bacon, & Hanlin comb. Nov.] tall fescue [ (Schreb.) Darbysh.]. The strategies presented include those 1) applied with forage systems, 2) based on pharmacological compounds and functional foods, and 3) based on supplemental dietary nutrients. Cattle BW gain and DM intake was the dependent response evaluated. Among the forage systems reviewed, studies with nontoxic, endophyte-infected tall fescue as a total replacement forage system demonstrated the greatest improvement in per-hectare (152 ± 27.5 kg/ha) and per-animal (0.29 ± 0.03 kg/d) BW gain. Studies with interseeded legumes have exhibited a small and highly variable BW gain effect size per hectare (52 ± 24.1 kg/ha) and per animal (0.11 ± 0.03 kg/d). The legume response was seasonal, with summer exhibiting the greatest benefit. Studies with chemicals that suppress plant growth demonstrated BW gain responses (0.17 ± 0.06 kg/d) equal to or greater than the response observed with legume studies. Cattle grazing toxic tall fescue responded well to anthelmentics, antimicrobial feed additives, and steroid implants, and the use of these technologies may additively help recover production losses. As a group, functional foods have not improved BW gain ( = 0.85). Studies with cattle supplemented with highly digestible fiber supplements observed a 0.15 kg greater BW gain compared with studies using starch- and sugar-based supplements ( < 0.05). Weight gain was positively impacted by the level of supplementation (0.06 kg/DM intake as percent BW). Supplement feed conversion was estimated at 6:1 for the highly digestible fiber supplements compared with 11:1 for starch-based supplements. Tall fescue forage DM intake was predicted to maximize at a supplemental feeding rate of 0.24% BW with a breakpoint at 0.5% BW, and total maximum DM intake (forage plus supplement) occurred at 2.7% BW when supplemental feeding approached 0.9% BW. Results from this meta-analysis should be useful for 1) establishing and comparing measured responses to theoretical improvements in BW gain when additive strategies are considered, 2) research planning, and 3) producer education.

Negative effects on cattle grazing tall fescue [Schedonorus arundinaceus (Schreb.) Dumort.] infected with the wild-type endophyte Neotyphodium coenophialum (E+) are well documented, but information about the carryover effects on weaned calves is limited. Our objective was to compare pre- and postweaning performance by spring-calving cows and calves grazing E+ with that by cows grazing a nontoxic endophyte–tall fescue association (NE+). Pregnant Gelbvieh × Angus crossbred cows (n = 136; 492 ± 19.2 kg of initial BW) were stratified by BW and age and allocated randomly to one of four 10-ha pastures in yr 1 (October 15) and one of eight 10-ha pastures in yr 2 (November 30). Pastures were allocated randomly before establishment to E+ or NE+. Cows remained on their assigned pastures until weaning in yr 2 but were removed from NE+ in the summer of yr 1 because of low forage mass. After weaning, calves grazed bermudagrass [Cynodon dactylon (L.) Pers.] followed by cool-season annual grasses. Cow BW and pregnancy rate, and calf weaning weight and preweaning gain were greater (P < 0.05) from NE+ versus E+. Weaning weight differentials were maintained throughout postweaning production phases, resulting in heavier HCW (P < 0.05) by steers and a tendency for greater (P < 0.10) subsequent calving rates by heifers. Therefore, replacing E+ with NE+ may improve preweaning cow and calf performance and heifer reproductive rates, but postweaning gains may not be affected by previous exposure to E+.

Sericea lespedeza (SL; Lespedeza cuneata) is a legume rich in condensed tannins that can be grazed or fed to small ruminants for parasite control. Condensed tannins, a secondary plant compound in SL, may lead to unintended consequences such as changes in production. In our preliminary research, there was consistently a reduction in serum and liver concentrations of Mo. The objective of this study was to determine the effect of SL with or without Mo supplementation on changes in BW, hematology, and serum biochemistry in lambs. Thirty ram lambs weaned in May (84 ± 1.5 d of age; 27 ± 1.1 kg) were blocked by BW, breed type (full or three-fourths Katahdin), and EBV of parasite resistance and randomly assigned to be fed 900 g of alfalfa-based supplement (CON; n = 10) or SL-based supplement (n = 20) for 103 d. Supplements were formulated to be isonitrogenous and isocaloric and to meet trace mineral requirements. Within the SL diet, half of the lambs received 490 mg sodium molybdate weekly (SLMO). Body condition scores and BW were determined every 14 d and blood and feces collected to determine hematological and serum biochemical profiles and fecal egg counts (FEC). Data were analyzed using a mixed model with repeated measures and orthogonal contrasts. The white blood cell counts tended to be reduced in SL- and SLMO-fed lambs compared with CON-fed lambs (P < 0.06), which was associated with a reduction in neutrophils (P < 0.001). Red blood cell counts were also reduced in SL but not SLMO lambs compared with CON lambs (P < 0.04). There was a reduction in blood packed cell volume (P < 0.04) and serum concentrations of albumin (P < 0.001) and creatinine (P < 0.02) in both SL and SLMO lambs compared with CON lambs. Similarly, concentrations of blood urea nitrogen were reduced in both SL and SLMO lambs, but differences among dietary treatments disappeared after 42 d of feeding (treatment × day, P < 0.004). Serum concentrations of total proteins were reduced only in SLMO lambs compared with other lambs (P < 0.001). Body weight and FEC were similar among dietary treatments. Means of all measurements were within a normal range, even though there were subtle but significant differences between dietary groups. Feeding a diet high in condensed tannin-rich SL did not lead to serious effects on hematology or serum biochemistry in lambs.

Coproduct feedstuffs offer a unique and potentially profitable avenue for cattle feeding strategies. However, research is lacking in the evaluation of varying coproducts on ruminal fermentation and digestive characteristics when included as the major component of the diet of cows. Our objective was to determine the effect of coproduct feedstuffs as a forage replacement on digestive and fermentative characteristics of cows. Eight ruminally fistulated cows (672 ± 32.0 kg initial BW and approximately 9 yr of age) were stratified by BW and randomly allocated to 1 of 4 diets (2 cows∙diet∙period) in a 2-period study: soybean hulls (SH), distillers' dried grains with solubles (DG), an isoenergetic mixture of soybean hulls and distillers' dried grains with solubles (MX), or ad libitum hay plus 0.9 kg/d of an isoenergetic mixture of soybean hulls and distillers' dried grains with solubles (HY). Diets were formulated to meet the ME requirements of a similar, companion study. Coproduct amounts were increased over a 14-d period. This was followed by a 14-d adaptation to diet and facilities and 5 d of total fecal collections. On the final day of fecal collections, rumen fluid was sampled immediately prior to feeding and 2, 4, 6, 8, 10 and 12 h after feeding for measurement of rumen VFA and ammonia concentrations. Intake of DM and OM was not different ( ≥ 0.28) among treatments, but digestibilities of DM, OM, NDF, and ADF were improved ( < 0.05) by coproduct feeding and by MX vs. the mean of SH and DG. Ruminal DM and OM fill were greater ( < 0.05) for cows offered HY than for cows offered the coproduct diets, greater for cows offered SH than for cows offered DG, and for the mean of SH and DG vs. MX. Ruminal retention time was greater ( < 0.05) for HY vs. the coproduct diets and for SH vs. DG. Apparent N absorption tended ( < 0.10) to be greater for cows offered the coproduct diets than for cows offered HY and greater for cows offered DG than for cows offered SH. Total VFA averaged across sampling times were greatest ( < 0.05) for cows offered SH, and ruminal ammonia N was greatest ( < 0.05) for cows offered either DG or MX at all sampling times. Based on these data, coproduct feedstuffs may be fed to meet the energy requirement of cows without negative effects on digestion or ruminal fermentation.

The objective of these experiments was to determine the effect of daily or alternate day supplementation of dried distillers grains with solubles (DDGS) with or without monensin on performance of grazing calves. Treatments included (1) a negative control with no supplemental feed except free-choice mineral; (2) DDGS offered daily at 1.02 kg as-fed per calf, including a mineral premix; (3) daily DDGS and monensin (Rumensin, Elanco, Greenfield, IN) at 1.02 kg as-fed per calf, including a mineral premix supplying 160 mg of monensin/calf daily; (4) alternate day DDGS to supply 2.04 kg as-fed per calf, including the mineral premix offered on alternate days; and (5) alternate day DDGS and monensin to supply 2.04 kg as-fed per calf, including a mineral premix and 320 mg of monensin/calf on alternate days. Four experiments were conducted which included both steers (n = 70) and heifers (n = 230) grazing warm season grass based pastures (Exp. 1, 2, and 3) or nontoxic endophyte-infected tall fescue (Exp. 4). Supplementation increased ADG (P ≤ 0.04) by 0.16 to 0.26 kg/d in the 4 studies, with an average improvement (P

Seven hectares (ha) of pearl millet [Pennisetum glaucum (L.) R. Br.] were divided into 3 blocks with 3 whole plots each to evaluate the effects of moisture (165, 187, and 274 g/kg of DM) and sampling depth (0.2, 0.4, and 0.6 m) in round bales on chemical composition and in situ disappearance. Plots were assigned randomly to moisture treatments. Round bales (n = 3 per plot; 1.2 × 1.5 m, 568 kg) were obtained and sampled at 3 different depths within each bale (n = 81) before and after 71 d of storage. Duplicate in situ bags (10 × 20 cm) were incubated in 6 ruminally cannulated cows (BW = 585 ± 37.8 kg) up to 120 h. Residual DM for each sampling time was fit to a nonlinear model using PROC NLIN of SAS to determine DM degradation kinetics. Data were analyzed as a split-plot design using Proc Mixed procedures of SAS and tested for moisture, depth, and their interactions. Bale temperature and concentrations of ADL were greater (P 0.10) any of the digestion variables, but the water-soluble fraction, potentially degradable fraction, and effective ruminal disappearance as well as concentrations of OM, NDF, and acid-detergent insoluble nitrogen were affected (P < 0.05) by the moisture × sampling depth interaction. Therefore, a greater range in sampling depth should be used to accurately assess heat damage in large round bales.

Dietary manipulations to include tannins can change the proportion and amounts of N excreted in the urine and feces as well as improve N-use efficiency in ruminants. This study was conducted to investigate effects on animal digestion parameters of adding different proportions of sericea lespedeza hay (SL) to alfalfa silage. Alfalfa was harvested in June 2018 at 75% bloom, chopped, and then packed at 55% moisture into plastic-lined bins and allowed to ensile for 3 months. Alfalfa silage was either offered alone or mixed with 9, 18, or 27% SL on a DM basis. These diets were offered randomly for ad libitum consumption to 16 ewes (41.8 ± 4.61 kg BW) in a randomized complete block design experiment with 2 periods to provide 4 observations per treatment per experimental period, each consisting of a 14-d dietary adaptation period followed by 5 d of total fecal and urine collection. Data were analyzed using PROC MIXED of SAS and orthogonal linear and quadratic trend analyses were tested. Digestibility (%) of DM and OM and digestible DM and OM intake (g/kg BW) decreased linearly (P < 0.05) with increasing SL addition to the diet. Digestibility of NDF and ADF decreased linearly (P < 0.01) with increasing SL, and apparent absorption (%) of N decreased linearly (P < 0.05) with increasing SL in the diet. Urinary N excretions (g/d) tended (P = 0.10) to decrease linearly while fecal N (g/day) tended to increase (P = 0.10) linearly with increasing SL proportion in the diet. In this study, supplementation with sericea lespedeza as a tannin source to alfalfa silage decreased forage digestibility and digestible organic matter intake and did not positively influence nitrogen use. The study was supported in part by USDA-ARS specific cooperative agreement 58-3655-4-052 and by USDA NIFA grant 2018-67019-27804.

Silage intake by ruminants can be affected by both fertilization and forage type. The objective of this study was to evaluate digestion by sheep offered three different grasses fertilized with either urea (C) or dairy slurry (S). Plots of meadow fescue (MF), tall fescue (TF) or orchardgrass (OG) were planted on 25 July 2017, and first harvested on 29 June 2018. Plots were then fertilized with urea (52 kg N/ha) or slurry (71,150 L/ha). A second harvest was baled 6 August at approximately 57% moisture and wrapped in plastic. Eighteen lambs (59 ± 1.5 kg) were allocated randomly to one of the 6 treatment combinations to provide 3 lambs per treatment within each of 3 periods consisting of a 10-d adaptation and 7-d total fecal and urine collection. Data were analyzed as a 2 × 3 factorial arrangement using PROC MIXED of SAS. Dry matter (DM) and organic matter (OM) intakes (g/kg BW) were greater (P < 0.05) from MF fertilized with C than from the other forage-fertility combinations. Digestibility of DM and OM were greater (P < 0.05) from MF vs. OG, and OG vs. TF. Digestibility of OM also was greater (P < 0.05) from C vs. S. Digestible DM and OM intakes (g/kg BW) were greatest (P < 0.05) from MF fertilized with C. In addition, digestible DM intake (g/kg BW) was greater (P < 0.05) for MF fertilized with S compared to TF fertilized with C, and digestible OM intake was greater (P < 0.05) for MF fertilized with S compared to OG fertilized with S. Therefore, urea-fertilized meadow fescue may improve energy intake compared with that from orchardgrass or tall fescue, but fertilization of meadow fescue with slurry may reduce this advantage. The study was supported in part by USDA-ARS specific cooperative agreement 58-3655-4-052.

Poor silage fermentation can affect its acceptance by livestock. Alfalfa from 3 field blocks were baled in large round bales (moisture concentration = 591 ± 43.0 g/kg) and then wrapped with 7 layers of plastic either with (EOB) or without (CW) an enhanced oxygen barrier on the day of baling, or 1, 2 or 3 d after baling in order to examine those effects on subsequent intake and digestibility by gestating sheep. Alfalfa was chopped after approximately 5 mo. of fermentation, and then offered for individual ad libitum consumption by 16 gestating ewes [63.5 ± 1.71 kg avg. body weight (BW)] in a 3-period (63-day) digestion study. Silage moisture and nitrogen (N) decreased linearly (P < 0.05), acid-detergent fiber (ADF) increased linearly (P < 0.05), and neutral-detergent fiber (aNDF) increased at a decreasing rate (linear; quadratic P < 0.05) with wrapping delay within EOB but not CW (P ≥ 0.23). Lactic acid expressed as g/kg of total silage acids decreased linearly (P < 0.05) within CW and decreased at an increasing rate (linear; quadratic P < 0.05) within EOB with increasing wrapping delay, likely because of aerobic deterioration. Digestible organic matter intake (DOMI) decreased linearly (P = 0.03) within EOB and quadratically (P = 0.02) within CW (wrap type × wrapping time after baling interaction; P = 0.04), but other intake and digestibility measurements were not affected (P ≥ 0.15) by wrap type or its interaction with wrapping time after baling. Intake of DM and OM (DMI and OMI, g/kg BW) as well as digestible DMI (g/kg BW) responded linearly and quadratically (P ≤ 0.03) with wrapping delay after baling by initially increasing to 1 d after baling, then declining sharply thereafter. Digestibility of aNDF increased linearly (P = 0.04) with wrapping delay which was likely related to reduced DMI. These values align somewhat closely with those for forage quality and fermentation profiles. Therefore, managing alfalfa silage to ensure more desirable fermentation should also result in higher digestible OMI, which will improve the overall energy status of ruminants.

Crabgrass ( Digitaria ciliaris [Retz.] Koel.) is a high-quality warm-season annual that can be used as hay, but field curing time may be lengthy compared with other forages. A 1.6-ha field of common crabgrass was divided into 12 plots (8.25 m × 50 m) that were used in a randomized complete block design with a 2 × 2 factorial treatment arrangement to determine the effects of a non-viable Lactobacillus –lactic acid additive and moisture concentration at baling on heating characteristics and pre- and post-storage chemical composition. Half of the plots within each block were treated with 0.081 ml/mg dry matter (DM) of a solution containing 110 g lactic acid/kg and non-viable Lactobacillus acidophilus at the time of mowing (T) and half were not treated (U). Within T and U plots, half were baled in small square bales at 163 g moisture/kg DM (L) and half at 251 g moisture/kg DM (H) of moisture. Six bales per plot were selected at random, weighed, and stored in separate insulated 6-bale stacks. Core samples were taken from 3 bales initially and 3 bales post-storage. Initial bale moisture concentrations were greater (P vs. L. Post-storage concentrations of NDF and acid-detergent insoluble N (ADIN; g/kg DM and g/kg N), were greater (P vs. L. Dry matter intake in sheep was unaffected (P>0.05) by either treatment, but DM digestibility and digestible DM intake were both positively affected (P L. In situ DM disappearance was largely affected by spray treatment × moisture interactions (P

Unstable weather, poor drying conditions, and unpredictable rainfall events often place valuable hay crops at risk. Recent research with large round bales composed of alfalfa (Medicago sativa L.) and orchardgrass (Dactylis glomerata L.) has shown that these large-bale packages are particularly sensitive to spontaneous heating and dry matter (DM) losses, as well as other undesirable changes with respect to forage fiber, protein, and energy density. Various formulations of organic acids have been marketed as preservatives, normally for use on hays that are not desiccated adequately in the field to facilitate safe bale storage. Our objectives for this study were to (1) evaluate the efficacy of applying a commercial (buffered) propionic acid-based preservative at 3 rates (0, 0.6, and 1.0% of wet-bale weight) to hays baled at 3 moisture concentrations (19.6, 23.8, and 27.4%) on the subsequent storage characteristics and poststorage nutritive value of alfalfa-orchardgrass forages packaged in large rectangular (285-kg) bales, and then (2) evaluate the in vivo digestibility of these hays in growing lambs. Over a 73-d storage period, the preservative was effective at limiting spontaneous heating in these hays, and a clear effect of application rate was observed for the wettest (27.4%) bales. For drier hays, both acid-application rates (1.0 and 0.6%) yielded comparable reductions in heating degree days >30°C relative to untreated controls. Reductions in spontaneous heating could not be associated with improved recovery of forage DM after storage. In this study, most changes in nutritive value during storage were related to measures of spontaneous heating in simple linear regression relationships; this suggests that the modest advantages in nutritive value resulting from acid treatment were largely associated with perturbations of normal heating patterns during bale storage. Although somewhat erratic, apparent digestibilities of both DM (Y=-0.0080x + 55.6; R(2)=0.45) and organic matter (Y=-0.0085x + 55.5; R(2)=0.53) evaluated in growing lambs were also directly related to heating degree days in simple linear relationships. Based on these data, applying propionic acid-based preservatives to large rectangular bales is likely to provide good insurance against spontaneous heating during storage, as well as modest benefits with respect to nutritive value and digestibility.

A total of 120 Gelbvieh × Angus crossbred heifers (552 ± 2.5 lb initial BW) grazed pastures of common bermudagrass [Cynodon dactylon (L.) Pers.] overseeded with wheat (Triticum aestivum L.) and annual ryegrass (Lolium multiflorum Lam.) for a 3-year study to compare the effect of seeding dates and tillage intensities on heifer growth performance. Half of the pastures were seeded in early September (Early) and half in mid October (Late). Within seeding date, half of the pastures were disked once (1×) and half were disked twice (2×) before seeding. Grazing began when forage mass reached approximately 2000 lb/acre and continued through 11 May 2002 (year 1), 25 April 2003 (year 2), and 10 May 2004 (year 3). Forage mass was greater from Early than from Late seeded pastures for 2 of the first 3 months of grazing resulting in 17-day earlier grazing initiation and approximately 165 lb less hay fed per heifer. Total body weight gain, or gain while grazing cool-season annuals did not differ between seeding dates or tillage intensities. Producers in the Mid South region with bermudagrass pastures may have considerable flexibility in their decisions as to when to seed annual forages and to what intensity they till their sod depending upon how soon they need available forage.

Tall fescue toxicosis adversely affects calving rate and weight gains reducing returns to cow-calf producers in the south–central United States. This grazing study estimated animal and economic performance implications of endophyte-infected fescue and calving season. Establishing novel endophyte-infected tall fescue on 25% of pasture acres resulted in improved calving rates (87% vs. 70%), weaning weights (532 lbs vs. 513 lbs), and partial returns per acre ($257 vs. $217). Additionally, fall-calving cows had higher calving rates (91% vs. 67%), weaning weights (550 lbs vs. 496 lbs), and partial returns per acre ($269 vs. $199) than spring calving cows.

a b s t r a c t Bermudagrass (Cynodon dactylon (L.) Pers.) is commonly used for grazing and haying in the southern USA, but hay curing can be challenging due to frequent rainfall events dur- ing spring and early summer. An existing stand of 'Greenfield' bermudagrass was divided into 12 plots using a randomized complete block design with a 2×2 factorial treatment arrangement to evaluate the influence of a non-viable Lactobacillus-lactic acid preservative and moisture concentration at baling on chemical composition, intake by sheep, and in situ disappearance in cattle. At time of mowing, half of the plots in each block were either spray-treated (T) or not treated (U) with 81 mL/t forage dry matter (DM) of the preser- vative solution. Hay was then baled at target moisture concentrations of either 174 g/kg DM (L) or 267 g/kg DM (H). Maximum temperature and heating degree days were greater (P 0.10). Post-storage nutritive value was largely influenced by moisture treatments only. Intake and digestibility, and in situ DM dis- appearance of these same hays were determined using 16 wether lambs (43 ± 3.7 kg initial BW), or six ruminally cannulated cows (617 ± 3.5 kg initial BW), respectively. Dry matter intake by sheep was not affected by either treatment factor (P>0.05), but DM digestibil- ity and digestible DM intake were greater (P

Relating single-nucleotide polymorphisms (SNP) to cows with acceptable productivity could benefit cattle breeders in areas where tall fescue is the predominant forage. This study aimed to (i) identify SNPs in bovine cytochrome P450 3A28 (CYP3A28) and (ii) determine the associations between SNP genotype, forage and cow body condition (BC). Genotype (CC, CG or GG) and forage [Kentucky-31 wild-type endophyte-infected tall fescue (KY+) vs. bermudagrass] effects on milk volume and quality were determined in Herd 1 cows (123 cows); in Herd 2 (99 cows), genotype and BC (low vs. moderate) effects on ovarian follicle size, calving date and calving per cent were determined; and in Herd 3 (114 cows), effects of genotype and fescue cultivar [KY+ vs. non-toxic endophyte-infected tall fescue (HiMag4)] were related to calving per cent, calving date and weaning weights of both cow and her calf. A cytosine (C) to guanine (G) transversion at base 994 (C994G) in CYP3A28 was identified. There was a genotype × forage type interaction (p < 0.05) on milk protein in Herd 1 cows; CC cows grazing bermudagrass had greater milk protein percentage in relation to other cows in the herd. In Herd 2, BC and genotype × BC tended (p < 0.10) to influence follicle size and Julian calving date respectively. Diameter of the largest follicle tended to be larger in moderate BC than in low-BC cows; whereas, CC and CG cows in moderate BC and homozygous (CC and GG) cows in low BC tended to calve 14 days earlier in relation to CG cows in low BC. In Herd 3, there was a genotype × forage type interaction (p < 0.05) on calving per cent, Julian calving date and calf weaning weight. In this study, genetic alterations (G allele at C994G) coupled with nutritional factors (low BC and toxic tall fescue) resulted in overall lower productivity in cows.

Fall-born calves grazing Neotyphodium coenophialum-infected tall fescue [E+; Lolium arundinaceum (Schreb.) Darbysh.] pastures should benefit from early weaning because of reduced exposure to fungal toxins. However, fall-born calves that grazed E+ and were weaned in mid-April had reduced post-weaning performance compared with calves managed similarly but weaned in early June. Gelbvieh × Angus calves (n = 238) were used in a 3-year study to determine the optimal time to wean fall-born calves grazing E+. Cow/calf pairs were allocated randomly to one of four weaning date treatments: 1) March 16 (177 ± 4.7 days of age; MarW), 2) April 13 (204 ± 4.7 days of age; AprW), 3) May 11 (236 ± 4.7 days of age; MayW), and 4) June 8 (264 ± 4.8 days of age; JuneW). On their assigned weaning date, calves were weighed, vaccinated, blood samples were collected, and calves were moved to 3.2-ha pastures adjacent to their dams for 14 days. After the weaning period, calves were weighed, blood samples were collected, and then calves were moved to pastures containing non-toxic forages. Birth weight and calf weights on MarW, AprW, and MayW weaning dates did not differ (P ≥ 0.21) across weaning date treatments. However, calf weaning weights on their respective weaning dates, weight on JuneW and on June 22 (14 days following the JuneW), daily gain between birth and June 22, and weight change between MarW and JuneW increased linearly (P

A number of studies have reported performance by spring-calving cows grazing Neotyphodium -infected fescue [E+, Lolium arundinaceum (Schreb.) Darbysh.], but post-weaning calf performance from the majority of those studies is limited. Furthermore, both pre- and post-weaning information pertaining to fall-calving cows and their calves grazing E+ is particularly limited. A 3-year study was initiated in April 2000 near Batesville, AR (USA) to investigate the impact of rotational management (twice monthly vs. twice weekly) program and weaning date [mid-April (EARLY) vs. early June (LATE)] on production of fall-calving cow-calf pairs grazing E+ overseeded with ladino ( Trifolium repens L.), and red clover ( Trifolium pretense L), lespedeza ( Lespedeza stipulacea Maxim.), and crabgrass ( Digitaria sanguinalis (L.) Scop.). A secondary objective of the study was to evaluate calf performance both at weaning and after weaning until either breeding of the heifers or slaughter of the steers. Calves weaned later had greater ( P P P P P P ≥ 0.11) from LATE in the other 2 years. Initial and final feedlot BW and gain did not differ ( P ≥ 0.16) among previous treatments in 2 of 3 years. Therefore, carryover effects of weaning date or rotational grazing on calves previously grazing E+ tall fescue may be mitigated during a subsequent backgrounding period such that impacts on subsequent breeding or feedlot performance is minimized.

In Arkansas, maximizing fall forage production from cereal grains is important for optimizing growth of fall-weaned calves (Bos taurus). Our objectives were to evaluate eight cultivars of wheat (Triticum aestivum L.), oat (Avena sativa L.), rye (Secale cereale L.), and triticale (x Triticosecale Wittmack) for their potential to accumulate fall forage dry matter (DM). At Fayetteville during 2004, triticale and oat cultivars accumulated DM in a cubic (P≤0.01) pattern over time, most likely because growing tillers exhibited stem elongation, and were then susceptible to freeze damage in late December. Wheat and rye cultivars accumulated DM in less complex patterns over harvest dates, but the maximum yield for any wheat cultivar was only 2554 kg ha -1 compared to 4661 kg ha -1 for oat. For Batesville 2004 and Fayetteville 2005, DM yields ranked similarly, but respective mean yields (491 and 988 kg ha -1 ) averaged over all harvest dates were only 25 and 50% of those for Fayetteville 2004 (1960 kg ha -1 ), largely due to drought. For Batesville 2005, growing conditions were somewhat unique relative to other site-years, and wheat, rye, and triticale cultivars accumulated an overall mean of 4148 kg ha -1 of forage DM on the final (4 January) harvest date. In contrast, oat cultivars were sensitive to freezing temperatures in November, and ranked last among all cultivars for yield on the final harvest date. Producers can usually improve fall forage production with cultivars that exhibit some stem elongation when planted in early September; however, this trait may make winter survival problematic.

Fertilizing bermudagrass (Cynodon dactylon L. Pers.) with N can produce large quantities of forage and increase plant N concentrations. Bermudagrass growing on a caged-layer manure-amended site was fertilized with ammonium nitrate at 4 rates (0, 56, 112, and 168 kg N/ha) approximately 1 mo before first and third harvests on May 30 and August 18, 2000, to determine in situ disappearance kinetics of N and neutral detergent insoluble N (NDIN). Five crossbred ruminally cannulated steers (BW = 422 ± 21.0 kg) were used to evaluate in situ disappearance kinetics in a randomized complete block design with a 2 × 4 factorial arrangement. Immediately soluble N (fraction A) and rate of N disappearance increased linearly (P

Throughout the southern Ozark Highlands, endophyte-infected tall fescue (Lolium arundi- naceum (Schreb.) Darbysh) hay often is stored outdoors, without cover. At two research sites (Fayetteville and Batesville, AR), the effects of unprotected storage were assessed for large round bales of tall fescue hay packaged at three diameters (≈1.1, 1.4, and 1.7 m). Bales were stored over winter either inside or outside on wooden pallets and then sampled at three depths (0-0.15 m, 0.15-0.31 m, and 0.31-0.46 m). At both locations, bale diameter had no effect (p > 0.05) on dry matter (DM) recovery or nutritive value. Generally, there was little dete- rioration of nutritive value during the storage period, regardless of treatment; however, some interactions (p ≤ 0.041) of storage location and sampling depth were observed at each experi- mental site. Ruminal disappearance kinetics of DM exhibited some statistical differences (p ≤ 0.030) in response to treatment; however, their relative magnitude was generally small, and there was little evidence to suggest biologi- cal relevance. After storage, concentrations of ergovaline were not affected (p > 0.05) by baling treatment at Batesville (overall mean 256 μg kg −1 ); however, this was a 27.3% reduction from the initial concentration immediately after mowing. At Fayetteville this reduction was even greater, falling by 79.4% between standing forage (539 μg kg −1 ) and samples taken from baled hay after storage (111 μg kg −1 ). At both sites, bales stored outside on wooden pallets exhibited relatively small changes in nutritive value, disappearance kinetics, and ergovaline at the bale surface rela- tive to the internal portions of the bale.

Two tall fescue (Festuca arundinacea Schreb.) forages, one an experimental host plant/endophyte association containing a novel endophyte (HM4) that produces low or nil concentrations of ergot alkaloids, and the other a typical association of Kentucky 31 tall fescue and the wild-type endophyte (Neotyphodium coenophialum; E+), were autumn-stockpiled following late-summer clipping and fertilization with 56 kg/ha of N to assess the nutritive value and ruminal disappearance kinetics of autumn-stockpiled tall fescue forages. Beginning on December 4, 2003, sixteen 361 +/- 56.4-kg replacement dairy heifers were stratified by weight and breeding and assigned to one of four 1.6-ha pastures (2 each of E+ and HM4) that were strip-grazed throughout the winter. Pastures were sampled before grazing was initiated (December 4), each time heifers were allowed access to a fresh strip (December 26, January 15, and February 4), and when the study was terminated (February 26). For fiber components, there were no interactions between fescue type and sampling date for either pregrazed or postgrazed forages. Over sampling dates, neutral detergent fiber (NDF; 56.5 to 67.8%), acid detergent fiber (27.7 to 34.9%), hemicellulose (28.8 to 34.0%), cellulose (25.0 to 28.1%), and lignin (3.61 to 10.05%) varied with sampling date, but patterns were almost exclusively curvilinear with time. Ruminal disappearance rate of dry matter (DM) was not affected by any treatment factor (overall mean for both pregrazed and postgrazed forages = 0.050 h(- 1)); similar responses were observed for NDF disappearance (overall mean = 0.048 h(- 1)). Interactions of fescue type and sampling date were observed for both pregrazed and postgrazed forages with respect to effective ruminal disappearance of DM; however, estimates were relatively high for all forages (overall mean = 64.0%). Effective disappearance of NDF was relatively extensive for all forages (overall mean = 55.4% of NDF). Based on the results of this trial, the endophyte status of stockpiled tall fescue forages had little practical effect on forage nutritive value and kinetics of ruminal DM or NDF disappearance. Overall, autumn-stockpiled tall fescue forages would appear to be a legitimate and lower cost alternative to harvested forages, and appear to possess suitable nutritional characteristics for developing dairy heifers in the Ozark Highlands.

The economic impact of rotation frequency (twice weekly vs. twice monthly until time of weaning) and time of weaning (early April vs. 56 d later) was examined for fall-calving cows using a modified put and take grazing strategy implemented to maintain equal grazing pressure across production systems. Cow and/or calf death losses resulted in replacements with either bred cows or cow-calf pairs. While the analysis on heifer calves was limited to sale at time of weaning, steer calves were tracked through the feedlot stage to determine if the time of sale would alter production system recommendations. For the conditions presented in this study, cow-calf producers may be advised that (i) relative to late weaning, early weaned calves did not regain their weight disadvantage observed at time of weaning; (ii) more intensive pasture management (rotating twice weekly rather than twice monthly) was not worth the effort because partial return results for late-weaned steer and heifer calves did not differ statistically by rotation frequency, regardless of sale time; (iii) partial returns were highest for steer calves retained through the finishing stage at the feedlot (regardless of production system) compared with sale at the time of weaning or at the time of placement in the feedlot; (iv) the range of returns or financial risk exposure increased with length of calf ownership. However, use of long-term average prices excluded input price risk from the analysis and output price risk was only partially addressed.

Background The effect of time of fecal sampling on the accuracy of acid-detergent insoluble ash (ADIA) and alkaline-peroxide lignin (APL) for the prediction of fecal output (FO) in cattle was evaluated. Eight ruminally cannulated cows (594 ± 35.5 kg) were allocated randomly to 4 bermudagrass [Cynodon dactylon (L.) Pers.] hay diets markedly different in crude protein concentration (79–164 g/kg) with 2 replicates per diet for 3 periods. Cows were offered hay individually at 20 g DM/kg of body weight daily in equal feedings at 08:00 and 16:00 h for a 10-d adaptation period followed by 5-d of total fecal collection. Fecal grab samples also were taken each day during the fecal collection period at 06:00, 12:00, 18:00, and 24:00 h either directly from the rectum or from freshly voided feces. Samples were composited within cow and time across the 5 d total fecal collection period. Additionally, forage, ort, and fecal samples were analyzed for concentrations of APL and ADIA. Results Fecal concentrations of ADIA and APL were not affected by sampling time (P ≥ 0.22), even though diet affected (P

A 4-yr trial was initiated in January 2000 to evaluate cow-calf performance on mixed-species pasture systems consisting of (i) endophyte-infected tall fescue (E+; Festuca arundinacea Schreb.) diluted by approximately 50% with common bermudagrass [Cynodon dactylon (L.) Pers.] and other forages; (ii) endophyte-free tall fescue (E-) overseeded into dormant common bermudagrass; and (iii) orchardgrass (OG; Dactylis glomerata L.) established under the same conditions as E-. The E- and OG pastures were grazed with either twice weekly (2W) or twice monthly (2M) rotation schedules, while pastures with E+ were grazed with 2M only. Actual weaning weights tended to be greater (P = 0.096), and age-adjusted 205-d weaning weights and average daily gain from birth to weaning were greater (P ≤ 0.035) for calves raised on low-toxicity (E - or OG) pastures compared to those raised on E+. Over 4 yr, calves raised on low-toxicity pastures exhibited 22- and 24-kg advantages in actual and 205-d adjusted weaning weights, respectively, compared to those raised on E+. Cows grazing OG and E- pastures exhibited greater (P ≤ 0.021) body weights and body condition scores (BCS) at calving than cows grazing E+ pastures. Furthermore, reductions in body weight and BCS between calving and weaning tended to be greater (P ≤ 0.088) for cows grazing E+ pastures. Calf performance was improved consistently by these low-toxidty pasture systems, but management requirements may limit adaptation by producers.

A systems trial was designed to evaluate forage characteristics within mixed-species pastures consisting of (i) endophyte-infected tall fescue (Festuca arundinacea Schreb.; E+) mixed with common bermudagrass [Cynodon dactylon (L.) Pers.] and other forages; (ii) endophyte-free tall fescue (E-) overseeded into dormant common bermudagrass; and (iii) orchardgrass (OG; Dactylis glomerata L.) established under the same conditions as E-. The E- and OG pastures were grazed with either twice weekly (2W) or twice monthly (2M) rotation schedules, but E+ was grazed only as 2M. Across 41 sampling dates (2000 through 2003) the mean forage mass across all forage systems was 3809 kg ha -1 , and there was an interaction of forage system and sampling date (P = 0.001). In vitro dry matter disappearance (IVDMD) and crude protein (CP) varied (P < 0.0001) with sampling date in seasonal patterns that were generally predictable. Frequencies of tall fescue in E- and E+ pastures increased (P < 0.10) over years, reaching numerical maxima (61 to 72%) at the end of the trial. For OG, frequencies reached numerical maxima of 52 and 42% in 2W and 2M pastures, respectively, but then declined (P < 0.10) over time, ending at 39 and 24%, respectively. At the end of the trial, reinfection of OG pastures by rogue E+ plants reached a numerical maximum frequency of only 10%, and concentrations of total ergot alkaloids in tall fescue plants from E- pastures were only about 25% of those for E+ pastures, thereby suggesting that pasture toxicity can be reduced substantially for at least 5 yr using these alternative forage systems.

‘Common’ and ‘Tifton 44’ bermudagrass [Cynodon dactylon (L.) Pers.] located near Fayetteville and Batesville, AR, respectively, were chosen to evaluate the effects of stockpiling initiation date (August or September), and N fertilization rate (0, 37, 74, or 111 kg N ha 21 )o n the nutritive value of fall-stockpiled bermudagrass. At the Fayetteville location, there were initiation 3 harvest date interactions for acid detergent fiber (ADF, P 5 0.003), hemicellulose (P 5 0.003), cellulose (P 5 0.0003), lignin (P 5 0.007), and crude protein (CP, P 5 0.008) in 2000, and strong interactions (P # 0.001) for all response variables in 2001. Generally, neutral detergent fiber (NDF), ADF, cellulose, and lignin increased over harvest dates for both initiation dates in 2000, although increases for lignin were only numerical (P . 0.05) for the September initiation date. Similar responses were observed for the August initiation date in 2001, but fiber components for the September 2001 initiation date declined over time because of contamination by other winter-annual species. For August initiation dates in 2000 and 2001, in vitro organic matter disappearance (IVOMD) declined linearly (P # 0.002) over harvest dates, reaching respective minima of 330 and 361 g kg 21 . At the Batesville site, an N fertilization effect (P # 0.017) was observed for NDF, ADF, and CP in both 2000 and 2001. A similar effect was observed for lignin (P 5 0.001) and IVOMD (P , 0.0001) in 2000, and for cellulose (P 5 0.0004) during 2001. Fertilization with N at the initiation of stockpiling generally reduced fiber components, and increased CP; however, IVOMD was increased for 2000 only. As observed for the Fayetteville site, most fiber components increased over harvest dates, while IVOMD declined concomitantly. Generally, the nutritive value of stockpiled bermudagrass declines between mid-October and mid-December, and spring-calving beef cows in the Upper South may need to be supplemented with energy to maintain body condition in the late fall or early winter.