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4 results on '"Kengradomkit C"'

2. Seroprevalence of Cryptosporidium parvum infection of dairy cows in three northern provinces of Thailand determined by enzyme-linked immunosorbent assay using recombinant antigen CpP23

Author

Inpankaew, T., primary, Jittapalapong, S., additional, Phasuk, J., additional, Pinyopanuwut, N., additional, Chimnoi, W., additional, Kengradomkit, C., additional, Sunanta, C, additional, Zhang, G, additional, Aboge, G.O., additional, Nishikawa, Y., additional, Igarashi, I., additional, and Xuan, X., additional

Published
2009
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3. Serodiagnosis of Toxoplasma gondiiinfection in dairy cows in Thailand

Author

Inpankaew, T., Pinyopanuwut, N., Chimnoi, W., Kengradomkit, C., Sununta, C., Zhang, G., Nishikawa, Y., Igarashi, I., Xuan, X., and Jittapalapong, S.

Abstract

Toxoplasma gondiiis a zoonotic protozoan parasite of both medical and veterinary importance worldwide. The parasite can cause severe complications in immunocompromised individuals such as AIDS patients and transplant recipients, where up to 25 of patients will develop toxoplasmic encephalitis. Toxoplasmosis is a zoonosis that causes a public health concern in both developed and developing countries such as Thailand. Livestock development particularly in dairy cows of Thailand have been hampered by low production of milk and slow growth rate because of many pathogens including T.?gondii. The objective of this study was to evaluate the serodiagnostic tool to be used for detection of T. gondiiinfection in dairy cows of Thailand. During 2006-2007, the sera of 700 cows from 55 small farm holders of the highest number of dairy cow population in the northern provinces were collected and analysed. Antibodies to T. gondiiwere assayed by latex agglutination test (LAT), enzyme-linked immunosorbent assay (ELISA) and indirect fluorescent antibody test (IFAT). The overall prevalence of T. gondiiinfection in dairy cows was 9.4 (66700) by LAT and 17 (119700) by ELISA. Sixty-three seropositive samples by LAT (95.5) and 107 by ELISA (89.9) were confirmed by IFAT. These results demonstrated that LAT had the highest specificity for detection of T. gondiiinfection.

Published
2010
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4. Molecular cloning, sequence analysis, and immune recognition of Bm95 from Thai strains of Rhipicephalus (Boophilus) microplus.

Author

Jittapalapong S, Thanasilp S, Kengradomkit C, Sirinarukmit T, Kaewmongkol G, and Stich RW

Subjects
Animals, Base Sequence, Cloning, Molecular, DNA Primers, DNA, Complementary, Insect Proteins genetics, RNA, Messenger genetics, RNA, Messenger isolation & purification, Rhipicephalus genetics, Insect Proteins immunology, Rhipicephalus immunology
Abstract

The efficacy of existing vaccines against Thai strains of Rhipicephalus (Boophilus) microplus remains to be determined, and these vaccine antigens should be compared to homologues derived from local strains. The purpose of this study was to compare Bm95 from Thai R. microplus to the previously reported sequence from Argentinean ticks. mRNA was isolated from R. microplus midgut samples, and cDNA was amplified with Bm95-specific primers. The cDNA and protein sequences of Thai Bm95 were 94 and 92% identical, respectively, to Argentinean Bm95. Although the sequence was similar to Argentinean Bm95, there were 45-amino acid differences among the homologues. Amplicons encoding Bm95 were cloned into pPICZalphaA and expressed in Pichia pastoris strain KM71H. The recombinant plasmid Bm95 (rBm95) was reactive to bovine hyperimmune serum to Thai R. microplus midgut, indicating that the Thai rBm95 was immunogenic. Further work is needed to compare the efficacies of Thai and Argentinean rBm95 for protection of cattle against infestation with Thai R. microplus.

Published
2008
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