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Your search keyword '"Kekesi, Gabriella"' showing total 35 results
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35 results on '"Kekesi, Gabriella"'

34. Characterization of dopamine D 2 receptor binding, expression and signaling in different brain regions of control and schizophrenia-model Wisket rats.

Author

Szűcs E, Ducza E, Büki A, Kekesi G, Benyhe S, and Horvath G

Subjects
Animals, Disease Models, Animal, Protein Binding, Rats, Rats, Wistar, Cerebellum metabolism, Hippocampus metabolism, Prefrontal Cortex metabolism, Receptors, Dopamine D2 metabolism, Schizophrenia metabolism, Signal Transduction physiology
Abstract

In previous studies we have shown that a three-hit animal model of schizophrenia (Wisket rat) has several behavioral impairments related to the disorder along with altered mu-opioid (MOP) and cannabinoid (CB1) receptor signaling. As the dopamine hypothesis of schizophrenia is central to research in the field, the goal of the present study was to investigate dopaminergic D 2 receptor (D 2 R) functions (binding capacity, G-protein activation and expression) in several brain regions (hippocampus, prefrontal cortex, striatum, olfactory bulb, cerebellum, brainstem, cortex and diencephalon) of control (Wistar) and Wisket rats. It was found that the D 2 R mediated maximal activation of G-proteins was substantially higher in hippocampus, striatum and olfactory bulb membranes prepared from the Wisket than in control animals, which was accompanied with lower potency of the D 2 R-mediated G-protein activation. In contrast, enhanced potency was detected in the prefrontal cortex without changes in the maximal activation. In saturation binding assays the maximal binding capacity of D 2 Rs was higher in the model animals in cerebral cortex, striatum and lower in the brainstem, while no changes in the dissociation constant values were detected. The D 2 R mRNA expression showed a trend for greater level in the investigated areas, while the D 2 R protein expression was significantly higher of Wisket rats compared to Wistar animals in the hippocampus and in the prefrontal cortex but not in the cerebellum. This study proved that the Wisket animals show altered D 2 receptor expression and function which might be related to the schizophrenia-like symptoms., (Copyright © 2020 Elsevier B.V. All rights reserved.)

Published
2020
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35. Antinociceptive activity of Sempervivum tectorum L. extract in rats.

Author

Kekesi G, Dobos I, Benedek G, and Horvath G

Subjects
Analgesics administration & dosage, Analgesics therapeutic use, Animals, Dose-Response Relationship, Drug, Hot Temperature, Injections, Intraperitoneal, Injections, Spinal, Male, Plant Extracts administration & dosage, Plant Extracts therapeutic use, Rats, Rats, Wistar, Analgesics pharmacology, Crassulaceae, Pain prevention & control, Phytotherapy, Plant Extracts pharmacology
Abstract

The extract of Sempervivum tectorum L. (Crassulaceae) containing several flavonoids is widely used as an antiinflammatory agent in folk medicine. Previous studies have demonstrated that various flavonoids or flavonoid-containing plant extracts produce significant antinociception, but no data are available concerning their antinociceptive effect especially at the spinal level. The purpose of the present study was to investigate the antinociceptive activity of Sempervivum tectorum L. extract on acute and inflammatory pain sensitivity in awake rats. The pain sensitivity was assessed by the acute tail- flick test in intact rats and by the paw withdrawal test after carrageenan-induced inflammation using heat stimulus. The plant extract was administered intraperitoneally and intrathecally in rats. The intraperitoneal injection of a high dose of the extract (1000 mg/kg) significantly (p < 0.05) increased the paw withdrawal latency of the inflamed paw. The intrathecal administration (30-300 micro g) caused a small, but significant increase (10%-15%) in tail- flick latency. In the carrageenan-induced inflammatory model, the intrathecally applied extract (30-1000 micro g) significantly decreased, but did not relieve the thermal hyperalgesia. The results suggest that the spinal cord does not seem to play an important role in the antinociceptive effects of this plant extract., (Copyright 2003 John Wiley & Sons, Ltd.)

Published
2003
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