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3. Fifth International Serum Exchange Workshop for Insulin Autoantibody (IAA) Standardization

Author

Greenbaum, C. J., Wilkin, T. J., Palmer, J. P., Agopian, M. S., Arnaiz -Villena, A., Becker, D., Becker, F., Bosi, E., Bottazzo, G. F., Bouix, O., Colman, P., Dawkins, R. L., De Leiva, A., Dean, B. M., DiMario, U., Eisenbarth, G. S., Elliot, R. B., Gorus, F., Huang, W., Humbel, R., Julia, M. R., Karjalainen, J., Keilacker, H., Kuglin, B., Kwan, J., Levy -Marchal, C., MacLaren, N., McEvoy, R. C., Molenaar, J. L., Reinauer, K. M., Scherbaum, W., Sodoyez, J. C., Vialettes, B., Zanchetta, R., and Ziegler, A. G.

Published
1992
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5. Measurement of Insulin During Isolation and Purification from Animal Pancreas

Author

S. Knospe, Besch W, Manfred Ziegler, Keilacker H, and Hildebrandt R

Subjects
medicine.medical_specialty, Endocrinology, Diabetes and Metabolism, Insulin, medicine.medical_treatment, High insulin, Radioimmunoassay, General Medicine, Peptide hormone, Biology, Endocrinology, medicine.anatomical_structure, In vivo bioassay, Internal medicine, Internal Medicine, medicine, Bioassay, Pancreas, Hormone
Abstract

For the purpose of monitoring the yield of the insulin extraction procedure from animal pancreas three methods of insulin determination were compared, i.e. the mouse convulsion test, a radioreceptor assay (RRA) on rat fat cells and a radioimmunoassay (RIA) which was especially laid out for high insulin concentrations. In samples containing actually insulin in general all three methods provided comparable results. Notable differences were only found in proinsulin-containing material. Because of its simplicity and high reproducibility as well as the good agreement of its results with those obtained with the other assays, the RIA turned out to be the most suitable assay. On the other hand, the RRA should be useful in detecting molecular differences between the investigated insulin-like preparations and standard insulin.

Published
2009

6. The Fourth International Workshop on the Standardisation of Insulin Autoantibody Measurement

Author

Kolb, H., Arnaiz-Villena, A., de Beaufort, C. E., Becker, D., Becker, F., Betterle, C., Bosi, E., Colman, P., Dean, E. B., Eisenbarth, G. S., Elliott, R. B., Gorus, F., Hübinger, A., Karjalainen, J., Keilacker, H., Kwan, J., Levy-Marchal, C., Maclaren, N., Palmer, J. P., Reinauer, K. M., Riley, W., Scherbaum, W., Singh, G. J. P., Sodoyez, J. C., Thivolet, C. H., Vialettes, B., Wilkin, T. J., and Ziegler, A. -G.

Published
1990
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9. Antibody response to islet antigens in anti-CD4/prednisolone immune intervention of type 1 diabetes

Author

D. Michaelis, Keilacker H, Kohnert Kd, M. Ziegler, F. Emmrich, F. Laube, and B. Hehmke

Subjects
Adult, medicine.medical_specialty, Adolescent, medicine.drug_class, medicine.medical_treatment, Prednisolone, Clinical Biochemistry, Glutamate decarboxylase, Monoclonal antibody, Lymphocyte Activation, Islets of Langerhans, Antigen, Internal medicine, medicine, Humans, Insulin, Child, Autoantibodies, Type 1 diabetes, geography, geography.geographical_feature_category, biology, business.industry, Glutamate Decarboxylase, Autoantibody, Antibodies, Monoclonal, medicine.disease, Islet, Combined Modality Therapy, Endocrinology, Diabetes Mellitus, Type 1, Immunology, Antibody Formation, CD4 Antigens, biology.protein, Antibody, business, Follow-Up Studies
Abstract

Cytoplasmic islet cell antibodies, glutamic acid decarboxylase autoantibodies, spontaneous insulin autoantibodies, and insulin-induced antibodies were analyzed in a 1-year follow-up study of 12 newly diagnosed patients with insulin-dependent diabetes mellitus aged 14 +/- 2 years (range 7-20 years) who had been initially treated with either multiple injections of insulin alone (control group) or, in addition, anti-CD4 monoclonal antibody/prednisolone (treatment group). Despite individual variations in islet cell antibody titers, there were no significant differences in the prevalence or changes in the mean titers between the two groups. Glutamic acid decarboxylase autoantibodies remained almost unchanged, but correlated with levels of islet cell antibodies. While at initiation of treatment only 50% of the patients from both groups had spontaneous insulin autoantibodies, all patients developed insulin-induced antibodies upon conventional insulin therapy during the course of follow-up. This was not related to islet cell antibody or glutamic acid decarboxylase antibody levels. The insulin requirement was markedly reduced through the period of follow-up, but did not significantly differ between the two groups. A correlation between islet cell antibody levels and insulin requirement was observed in the control group but not in the treatment group. Plasma levels of the antibodies were not associated with changes in stimulated C-peptide or hemoglobin A1 concentrations. Activated T-lymphocytes persisted in both groups of patients, but their mean levels were not significantly different. The reason for the absence of statistically significant differences between treatment and control groups could be due to the small number of patients in the study. In conclusion, short-term immune intervention with anti-CD4 monoclonal antibody in addition to insulin therapy did not suppress autoimmune reactions towards the beta cells.

Published
1996

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