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A variety of seabird species migrate annually from wintering grounds in the Southern Hemisphere to the Gulf of Maine, USA to breed and raise their young. Post-migration, adult seabirds depend on the spatio-temporal match of reliable food resources to replenish energy reserves before breeding. However, the conditions during this critical window of time are becoming increasingly uncertain given the magnitude and pace at which climate change is impacting the Gulf of Maine region. We investigated the pre-breeding foraging ecology of Arctic Terns (Sterna paradisaea), Common Terns (S. hirundo), and the federally endangered Roseate Tern (S. dougallii) by analyzing stable carbon (δ13C) and nitrogen (δ15N) isotopes in eggshell tissues collected from seven islands in the Gulf of Maine from 2016 to 2018. Results show at the interspecific level, adult foraging patterns are consistent with expectations based on chick diets. At interisland and interannual scales, variation in isotopic values and niche breadths suggest foraging habits are highly localized. Although uncertainty remains, interannual trends also suggest warmer ocean conditions are either affecting tern foraging behaviors and/or prey resource availability during the late spring and early summer. Overall, results provide new information on adult tern foraging ecology in an important breeding area experiencing rapid environmental change.

Voltage-gated ion channels feature voltage sensor domains (VSDs) that exist in three distinct conformations during activation: resting, intermediate, and activated. Experimental determination of the structure of a potassium channel VSD in the intermediate state has previously proven elusive. Here, we report and validate the experimental three-dimensional structure of the human KCNQ1 voltage-gated potassium channel VSD in the intermediate state. We also used mutagenesis and electrophysiology in Xenopus laevisoocytes to functionally map the determinants of S4 helix motion during voltage-dependent transition from the intermediate to the activated state. Finally, the physiological relevance of the intermediate state KCNQ1 conductance is demonstrated using voltage-clamp fluorometry. This work illuminates the structure of the VSD intermediate state and demonstrates that intermediate state conductivity contributes to the unusual versatility of KCNQ1, which can function either as the slow delayed rectifier current (IKs) of the cardiac action potential or as a constitutively active epithelial leak current.

Increased production in smallholder beef systems requires improved forage management. Our objective was to evaluate the effects of composted cattle manure and mineral nitrogen (urea) application on soil fertility and partial nutrient balances in plots established to Brachiaria cv. Mulato II in south-central coastal Vietnam from 2010 to 2013. A randomized complete block design was implemented on six farms (blocks), with five rates of composted cattle manure (0, 4, 8, 12, and 24 Mg DM/ha per yr) and three urea rates (0, 60, and 120 kg N/ha per yr) in a factorial design. Soil was analyzed before and after the experiment. Compost increased soil pH, organic matter, Ca, Mg, and Mn. The effect of compost and urea applications on postexperiment soil fertility depended on preexperiment soil fertility for K, P, S, Mg, Zn, Mn, Cu, and organic matter, suggesting that the ability to maintain soil fertility depends on the interaction between soil organic and inorganic amendments and existing soil fertility. Highest farm yields were also achieved on farms with higher preexperiment soil fertility levels. Negative partial nutrient balances for N, P, and K suggest that yields will not be sustainable over time even for the highest fertilization inputs used in this experiment.

Microtubule-organizing centers (MTOCs) form, anchor, and stabilize the polarized network of microtubules in a cell. The central MTOC is the centrosome that duplicates during the cell cycle and assembles a bipolar spindle during mitosis to capture and segregate sister chromatids. Yet, despite their importance in cell biology, the physical structure of MTOCs is poorly understood. Here we determine the molecular architecture of the core of the yeast spindle pole body (SPB) by Bayesian integrative structure modeling based on in vivo fluorescence resonance energy transfer (FRET), small-angle x-ray scattering (SAXS), x-ray crystallography, electron microscopy, and two-hybrid analysis. The model is validated by several methods that include a genetic analysis of the conserved PACT domain that recruits Spc110, a protein related to pericentrin, to the SPB. The model suggests that calmodulin can act as a protein cross-linker and Spc29 is an extended, flexible protein. The model led to the identification of a single, essential heptad in the coiled-coil of Spc110 and a minimal PACT domain. It also led to a proposed pathway for the integration of Spc110 into the SPB.

The objective of this study was to describe associations between childhood factors and adult daily substance use profiles among homeless women and to identify independent predictors of daily substance use for the overall sample and for ethnic/acculturation subgroups. Structured surveys were administered to 1,331 homeless women in Los Angeles who were either daily drug and alcohol users for the past 6 months or nondaily substance users. Physical abuse and parental drug abuse predicted daily drug use in the whole sample and selected subgroups, whereas parental alcohol abuse predicted daily alcohol use in the whole sample. Teen self-esteem was also found to have a protective effect on daily alcohol use for the sample and for African American women. Negative peer influence in adolescence predicted daily drug use among high-acculturated Latinas. In summary, childhood abuse, parental substance use, and negative peer influence affect important roles in homeless women's daily substance use.

A qualitative research approach was used to explore the factors that influence the initiation of drug and alcohol use among homeless women and the health and social consequences of drug and alcohol use. The sample consisted of 238 women; of whom 209 women reported drug and/or alcohol use in the past month and 29 women reported no history of drug or alcohol use. Findings of the study revealed homeless women who currently used drugs and alcohol, homeless women who currently used drugs only, and to a lesser extent current alcohol users only, had suffered traumatic childhood events and family dysfunction and had to cope with low self-esteem, emotional distress, and poor physical health. The initiation of drug and/or alcohol use was strongly affected by the social influence of other users. In comparison, homeless women who did not use drugs or alcohol reported a positive self-image, few traumatic events, and chose partners who did not use drugs or alcohol. Common among current drug and/or alcohol users were the reported social benefits of drug use. Quantitative analyses revealed homeless women who were current drug users were significantly more likely to have experienced childhood and adult victimization as compared with women in the other groups. Thus, the need for social interventions and positive social support early in the lives of these women are strongly implicated in these findings.

On 28 April 2019, hourly forecasts from the operational High-Resolution Rapid Refresh (HRRR) model consistently predicted an isolated supercell storm late in the day near Dodge City, Kansas, that subsequently was not observed. Two convection-allowing model (CAM) ensemble runs are created to explore the reasons for this forecast error and implications for severe weather forecasting. The 40-member CAM ensembles are run using the HRRR configuration of the WRF-ARW Model at 3-km horizontal grid spacing. The Gridpoint Statistical Interpolation (GSI)-based ensemble Kalman filter is used to assimilate observations every 15 min from 1500 to 1900 UTC, with resulting ensemble forecasts run out to 0000 UTC. One ensemble only assimilates conventional observations, and its forecasts strongly resemble the operational HRRR with all ensemble members predicting a supercell storm near Dodge City. In the second ensemble, conventional observations plus observations of WSR-88D radar clear-air radial velocities, WSR-88D diagnosed convective boundary layer height, and GOES-16 all-sky infrared brightness temperatures are assimilated to improve forecasts of the preconvective environment, and its forecasts have half of the members predicting supercells. Results further show that the magnitude of the low-level meridional water vapor flux in the moist tongue largely separates members with and without supercells, with water vapor flux differences of 12% leading to these different outcomes. Additional experiments that assimilate only radar or satellite observations show that both are important to predictions of the meridional water vapor flux. This analysis suggests that mesoscale environmental uncertainty remains a challenge that is difficult to overcome. Significance Statement: Forecasts from operational numerical models are the foundation of weather forecasting. There are times when these models make forecasts that do not come true, such as 28 April 2019 when successive forecasts from the operational High-Resolution Rapid Refresh (HRRR) model predicted a supercell storm late in the day near Dodge City, Kansas, that subsequently was not observed. Reasons for this forecast error are explored using numerical experiments. Results suggest that relatively small changes to the prestorm environment led to large differences in the evolution of storms on this day. This result emphasizes the challenges to operational severe weather forecasting and the continued need for improved use of all available observations to better define the atmospheric state given to forecast models. [ABSTRACT FROM AUTHOR]