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The purpose of this study was to quantify differences in markers of cardiometabolic health with different frequency, intensity, and time (FIT) parameters to reduce sedentary behavior. The subjects consisted of 7 men and 6 women (with a mean age ± SD of 74.2 ± 7.8 yrs >6 hrs of sedentary behavior per day) who performed 4 treatments of sedentary interruption bouts (SIB) lasting one week each. The FIT of the SIB differed across each program. There were 1-wk long washout/control periods between each of the SIB treatments. Cardiometabolic risk factors were measured weekly. The subjects performed a consistent exercise training program throughout the intervention to permit examination of the independent effect of reducing sedentary behavior through different SIB programs on cardiometabolic health. SIB1 (every 60 min, 2 METs, 5 min) elicited a significant increase (P<0.05) in plasma HDL by 21.2%, decrease in plasma triglyceride by 24.6%, and decrease in blood glucose by 6.1%, compared to the control. SIB4 (every 120 min, 2 METs, 10 min) resulted in a significant increase (P<0.05) in plasma HDL by 18.4%, decrease in plasma triglyceride by 23.0%, and a decrease in blood glucose by 7.8%, compared to the control. Lastly, SIB2 (every 120 min, 2 METs, 5 min) and SIB3 (every 120 min, 3 METs, 5 min) elicited no significant (P>0.05) cardiometabolic changes when compared to the control. More frequent bouts of SIB (every 60 min) is most beneficial to cardiometabolic health. If the SIB is less frequent (such as >60 min), a longer duration of interruption bout activities (i.e., 10 min) is more likely to be favorable to cardiometabolic health. [ABSTRACT FROM AUTHOR]