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98 results on '"Kedes, Laurence H."'

10. DISCOVERING THE SECRETS OF DNA.

Author

Friedland, Peter and Kedes, Laurence H.

Subjects
*BIOLOGY, *ARTIFICIAL intelligence, *COMPUTER software
Abstract

Focuses on the contributions of artificial-intelligence methodologies in the development of software for biological science. Impact of the discovery of the genetic code on the understanding of biological processes; Discussion of the science of molecular genetics; Use of symbolic pattern-recognition tools in analyzing DNA sequences.

Published
1985
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11. A Novel Gene Delivery System Targeted to Breast Cancer Cells

Author

UNIVERSITY OF SOUTHERN CALIFORNIA LOS ANGELES, Kedes, Laurence H., Medina-Kauwe, Lali K., UNIVERSITY OF SOUTHERN CALIFORNIA LOS ANGELES, Kedes, Laurence H., and Medina-Kauwe, Lali K.

Abstract

We are producing a gene delivery vehicle that uses unique features of viral proteins in a targeted, non-viral gene delivery system. The key features of this system that will lend themselves to gene therapy are: 1) the missile-like targeting capacity; 2) the capacity to transport DNA, and therefore, therapeutic genes; 3) the lack of size restriction, and therefore, the potential capacity to carry any size gene; 4) the lack of viral genes, and therefore, virulence; and, 5) the enhanced ability to enter cells and deliver therapeutic genes. While we expect this system to retain some of the best properties of viral vectors while taking advantage of mechanisms that viruses have evolved to efficiently enter cells, we also expect this system to improve on these mechanisms by introducing cell type-specific targeting capabilities. Because this system is non-viral in nature, it avoids problems inherent in biologic agents, thus enabling pharmaceutical quality control over the final preparations. Potential application of this system includes, but is not limited to, the targeted delivery of therapeutic genes or drugs for cancer treatment or gene replacement therapy., The original document contains color images. All DTIC reproductions will be in black and white.

Published
2002

25. The organization of sea urchin histone genes

Author

Kedes, Laurence H., Cohn, Ronald H., Lowry, Jean C., Chang, Annie C.Y., and Cohen, Stanley N.

Abstract

Sucrose gradient analysis of total sea urchin DNA cleaved with theEcoRI andHindIII restriction endonucleases and identification of histone coding gene sequences by hybridization with histone mRNA have elucidated the basic organization of the histone gene repeat unit. These data, plus results obtained by electrophoretic analysis of purified endonuclease-cleaved sea urchin histone DNA and hybridization with cRNA transcribed from the eucaryotic segment of constructed plasmid chimeras cloned in E. coli, show that the several DNA sequences coding for individual histone proteins are intermingled in a 7 kilobase (kb) repeat unit. Cleavage of total sea urchin DNA withEcoRI produces 2.2 and 4.8 kb fragments which are homologous with the two cloned fragments, and which are contained in a 7 kbHindIII fragment. Cleavage with both enzymes reveals that the 2.2 kbEcoRI fragment contains aHindIII site 0.15–0.2 kb from an end. RNA · DNA hybridization between chimeric plasmid DNA and purified individual mRNAs isolated from sea urchin embryo polyribosomes has been used to assign coding sequences to either the 2.2 or 4.8 kb region of the histone DNA repeat unit. A map of the histone genes is proposed.

Published
1975
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