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Abstract Circadian rhythms are self-sustaining oscillations within biological systems that play key roles in a diverse multitude of physiological processes. The circadian clock mechanisms in brain and peripheral tissues can oscillate independently or be synchronized/disrupted by external stimuli. Dental enamel is a type of mineralized tissue that forms the exterior surface of the tooth crown. Incremental Retzius lines are readily observable microstructures of mature tooth enamel that indicate the regulation of amelogenesis by circadian rhythms. Teeth enamel is formed by enamel-forming cells known as ameloblasts, which are regulated and orchestrated by the circadian clock during amelogenesis. This review will first examine the key roles of the circadian clock in regulating ameloblasts and amelogenesis. Several physiological processes are involved, including gene expression, cell morphology, metabolic changes, matrix deposition, ion transportation, and mineralization. Next, the potential detrimental effects of circadian rhythm disruption on enamel formation are discussed. Circadian rhythm disruption can directly lead to Enamel Hypoplasia, which might also be a potential causative mechanism of amelogenesis imperfecta. Finally, future research trajectory in this field is extrapolated. It is hoped that this review will inspire more intensive research efforts and provide relevant cues in formulating novel therapeutic strategies for preventing tooth enamel developmental abnormalities.

Genome-scale metabolic models (GEMs) have been widely employed to predict microorganism behaviors. However, GEMs only consider stoichiometric constraints, leading to a linear increase in simulated growth and product yields as substrate uptake rates rise. This divergence from experimental measurements prompted the creation of enzyme-constrained models (ecModels) for various species, successfully enhancing chemical production. Building upon studies that allocate macromolecule resources, we developed a Python-based workflow (ECMpy) that constructs an enzyme-constrained model. This involves directly imposing an enzyme amount constraint in GEM and accounting for protein subunit composition in reactions. However, this procedure demands manual collection of enzyme kinetic parameter information and subunit composition details, making it rather user-unfriendly. In this work, we've enhanced the ECMpy toolbox to version 2.0, broadening its scope to automatically generate ecGEMs for a wider array of organisms. ECMpy 2.0 automates the retrieval of enzyme kinetic parameters and employs machine learning for predicting these parameters, which significantly enhances parameter coverage. Additionally, ECMpy 2.0 introduces common analytical and visualization features for ecModels, rendering computational results more user accessible. Furthermore, ECMpy 2.0 seamlessly integrates three published algorithms that exploit ecModels to uncover potential targets for metabolic engineering. ECMpy 2.0 is available at https://github.com/tibbdc/ECMpy or as a pip package (https://pypi.org/project/ECMpy/).

Abstract Iron-based Fischer–Tropsch synthesis (FTS) catalyst is widely used for syngas conversion, but its iron carbide active phase is easily oxidized into Fe3O4 by the water produced during reaction, leading to the deterioration of catalytic performance. Here, we show an efficient strategy for protecting the iron carbide active phase of FTS catalyst by surface hydrophobization. The hydrophobic surface can reduce the water concentration in the core vicinity of catalyst during syngas conversion, and thus inhibit the oxidation of iron species by water, which enhances the C − C coupling ability of catalyst and promotes the formation of long-chain olefins. More significantly, it is unraveled that appropriate shell thickness plays a crucial role in stabilizing the iron carbide active phase without Fe3O4 formation and achieving good catalytic performance.

Abstract A body area network involving wearable sensors distributed around the human body can continuously monitor physiological signals, finding applications in personal healthcare and athletic evaluation. Existing solutions for near-field body area networks, while facilitating reliable and secure interconnection among battery-free sensors, face challenges including limited spectral stability against external interference. Here we demonstrate a textile metamaterial featuring a coaxially-shielded internal structure designed to mitigate interference from extraneous loadings. The metamaterial can be patterned onto clothing to form a scalable, customizable network, enabling communication between near-field reading devices and battery-free sensing nodes placed within the network. Proof of concept demonstration shows the metamaterial’s robustness against mechanical deformation and exposure to lossy, conductive saline solutions, underscoring its potential applications in wet environments, particularly in athletic activities involving water or significant perspiration, offering insights for the future development of radio frequency components for a robust body area network at a system level.

Abstract Background Patients with atrial fibrillation (AF) often have coronary artery disease (CAD), but the biological link between them remains unclear. This study aims to explore the common pathogenesis of AF and CAD and identify common biomarkers. Methods Gene expression profiles for AF and stable CAD were downloaded from the Gene Expression Omnibus database. Overlapping genes related to both diseases were identified using weighted gene co-expression network analysis (WGCNA), followed by functional enrichment analysis. Hub genes were then identified using the machine learning algorithm. Immune cell infiltration and correlations with hub genes were explored, followed by drug predictions. Hub gene expression in AF and CAD patients was validated by real-time qPCR. Results We obtained 28 common overlapping genes in AF and stable CAD, mainly enriched in the PI3K-Akt, ECM-receptor interaction, and relaxin signaling pathway. Two hub genes, COL6A3 and FKBP10, were positively correlated with the abundance of MDSC, plasmacytoid dendritic cells, and regulatory T cells in AF and negatively correlated with the abundance of CD56dim natural killer cells in CAD. The AUCs of COL6A3 and FKBP10 were all above or close to 0.7. Drug prediction suggested that collagenase clostridium histolyticum and ocriplasmin, which target COL6A3, may be potential drugs for AF and stable CAD. Additionally, COL6A3 and FKBP10 were upregulated in patients with AF and CAD. Conclusion COL6A3 and FKBP10 may be key biomarkers for AF and CAD, providing new insights into the diagnosis and treatment of this disease.

Saccharomyces cerevisiae, a widely utilized model organism, has seen continuous updates to its genome-scale metabolic model (GEM) to enhance the prediction performance for metabolic engineering and systems biology. This study presents an auxotrophy-based curation of the yeast GEM, enabling facile upgrades to yeast GEMs in future endeavors. We illustrated that the curation bolstered the predictive capability of the yeast GEM particularly in predicting auxotrophs without compromising accuracy in other simulations, and thus could be an effective manner for GEM refinement. Last, we leveraged the curated yeast GEM to systematically predict auxotrophs, thereby furnishing a valuable reference for the design of nutrient-dependent cell factories and synthetic yeast consortia.

Abstract. Background:. Although overnight fasting is recommended prior to endoscopic retrograde cholangiopancreatography (ERCP), the benefits and safety of high-carbohydrate fluid diet (CFD) intake 2 h before ERCP remain unclear. This study aimed to analyze whether high-CFD intake 2 h before ERCP can be safe and accelerate patients' recovery. Methods:. This prospective, multicenter, randomized controlled trial involved 15 tertiary ERCP centers. A total of 1330 patients were randomized into CFD group (n = 665) and fasting group (n = 665). The CFD group received 400 mL of maltodextrin orally 2 h before ERCP, while the control group abstained from food/water overnight (>6 h) before ERCP. All ERCP procedures were performed using deep sedation with intravenous propofol. The investigators were blinded but not the patients. The primary outcomes included postoperative fatigue and abdominal pain score, and the secondary outcomes included complications and changes in metabolic indicators. The outcomes were analyzed according to a modified intention-to-treat principle. Results:. The post-ERCP fatigue scores were significantly lower at 4 h (4.1 ± 2.6 vs. 4.8 ± 2.8, t = 4.23, P

Abstract Purpose Some studies have found that the pathological formation of kidney stones is closely related to injury and inflammatory response. Behaviors such as dietary composition, physical activity, obesity and smoking can all affect the body’s oxidative stress levels. In order to evaluate the effects of various diets and lifestyles on the body’s oxidative and antioxidant systems, an oxidative balance score was developed. To investigate whether the OBS is associated with the development of kidney stones. Methods Data were taken from the National Health and Nutrition Examination Survey (NHANES) from 2007–2018, followed by retrospective observational studies. The association between kidney stones and OBS was analyzed using survey-weighted logistic regression by adjusting for demographics, laboratory tests, and medical comorbidity covariates. The oxidative balance score is calculated by screening 16 nutrients and 4 lifestyle factors, including 5 prooxidants and 15 antioxidants, based on prior information about the relationship between oxidation levels in the body and nutrients or lifestyle factors. Results A total of 26,786 adult participants were included in the study, of which 2,578, or 9.62%, had a history of nephrolithiasis. Weighted logistic regression analysis found an association between OBS and kidney stones. In the fully tuned model, i.e., model 3, the highest quartile array of OBS was associated with the lowest quartile array of OBS (OR = 0.73 (0.57, 0.92)) with the risk of kidney stone (p = 0.01), and was statistically significant and remained relatively stable in each model. At the same time, the trend test in the model is also statistically significant. With the increase of OBS, the OR value of kidney stones generally tends to decrease. Conclusions There is an inverse correlation between OBS and kidney stone disease. At the same time, higher OBS suggests that antioxidant exposure is greater than pro-oxidative exposure in diet and lifestyle, and is associated with a lower risk of kidney stones

Abstract Background Smoking induces and modifies the airway immune response, accelerating the decline of asthmatics’ lung function and severely affecting asthma symptoms’ control level. To assess the prognosis of asthmatics who smoke and to provide reasonable recommendations for treatment, we constructed a nomogram prediction model. Methods General and clinical data were collected from April to September 2021 from smoking asthmatics aged ≥14 years attending the People’s Hospital of Zhengzhou University. Patients were followed up regularly by telephone or outpatient visits, and their medication and follow-up visits were recorded during the 6-months follow-up visit, as well as their asthma control levels after 6 months (asthma control questionnaire-5, ACQ-5). The study employed R4.2.2 software to conduct univariate and multivariate logistic regression analyses to identify independent risk factors for ‘poorly controlled asthma’ (ACQ>0.75) as the outcome variable. Subsequently, a nomogram prediction model was constructed. Internal validation was used to test the reproducibility of the model. The model efficacy was evaluated using the consistency index (C-index), receiver operating characteristic (ROC) curve, calibration curve, and decision curve. Results Invitations were sent to 231 asthmatics who smoked. A total of 202 participants responded, resulting in a final total of 190 participants included in the model development. The nomogram established five independent risk factors (P

Proteins play a pivotal role in coordinating the functions of organisms, essentially governing their traits, as the dynamic arrangement of diverse amino acids leads to a multitude of folded configurations within peptide chains. Despite dynamic changes in amino acid composition of an individual protein (referred to as AAP) and great variance in protein expression levels under different conditions, our study, utilizing transcriptomics data from four model organisms uncovers surprising stability in the overall amino acid composition of the total cellular proteins (referred to as AACell). Although this value may vary between different species, we observed no significant differences among distinct strains of the same species. This indicates that organisms enforce system-level constraints to maintain a consistent AACell, even amid fluctuations in AAP and protein expression. Further exploration of this phenomenon promises insights into the intricate mechanisms orchestrating cellular protein expression and adaptation to varying environmental challenges.

Abstract The lining structures of tunnels are typically constructed using sprayed or cast concrete materials, and their performance and quality during tunnel excavation and blasting are crucial for the stability and safety of tunnels. Therefore, the safe distance between the lining structure and blasting source should be determined to avoid concrete damage caused by blasting vibrations. In this study, taking the subway tunnel of Danshan Station in Qingdao as an example, the JH-2 model is introduced as the constitutive model of the tunnel blasting simulation, and the JH-2 model parameters of the local surrounding rock are obtained by experiments, and finally the numerical simulation and theoretical verification are carried out to study the safety distance of shotcrete under various safety judgment standards. The results indicate that the JH-2 model can effectively simulate the propagation of stress waves under different media conditions, and the closer the strength parameters and pressure constant of the lining structure are to those of the surrounding rock, the safer the concrete–rock bonding interface. During tunnel blasting construction using the ring blasting method, the peak particle velocity (PPV) of the lining structure increases with an increase in the arch angle. Based on the numerical simulation results, we recommend that concrete lining be constructed at a distance of at least 62 m from the blasting source to avoid damage caused by vibrations. The effect of concrete tensile failure caused by longitudinal stress is much smaller than the damage to the bonding interface caused by the PPV and can be neglected.

Abstract The death of coronavirus disease 2019 (COVID-19) is primarily due to from critically ill patients, especially from ARDS complications caused by SARS-CoV-2. Therefore, it is essential to contribute an in-depth understanding of the pathogenesis of the disease and to identify biomarkers for predicting critically ill patients at the molecular level. Immunogenic cell death (ICD), as a specific variant of regulatory cell death driven by stress, can induce adaptive immune responses against cell death antigens in the host. Studies have confirmed that both innate and adaptive immune pathways are involved in the pathogenesis of SARS-CoV-2 infection. However, the role of ICD in the pathogenesis of severe COVID-19 has rarely been explored. In this study, we systematically evaluated the role of ICD-related genes in COVID-19. We conducted consensus clustering, immune infiltration analysis, and functional enrichment analysis based on ICD differentially expressed genes. The results showed that immune infiltration characteristics were altered in severe and non-severe COVID-19. In addition, we used multiple machine learning methods to screen for five risk genes (KLF5, NSUN7, APH1B, GRB10 and CD4), which are used to predict COVID-19 severity. Finally, we constructed a nomogram to predict the risk of severe COVID-19 based on the classification and recognition model, and validated the model with external data sets. This study provides a valuable direction for the exploration of the pathogenesis and progress of COVID-19, and helps in the early identification of severe cases of COVID-19 to reduce mortality.

Asphalt could suffer hydrothermal ageing in underwater tunnels, which varies its combustion characteristics significantly. The hydrothermally aged asphalt (HA) was comparatively studied with base asphalt (BA) and thermal-oxidatively aged asphalt (TA). Major findings are achieved: (1) The C＝O and S＝O bonds are more abundant in TA/HA than BA. HA contains more prominent –OH and H2O but less substantial aliphatics. And it has lower SO than TA by about 20 %, attributing to the oxygen isolation of aqueous immersion during hydrothermal ageing. (2) Remarkably bimodal heat release rate (HRR) peaks are found for HA, while only unimodal HRR peak is observed for TA/BA. Importantly, the primarily main HRR peak of HA occurs at around 110 s, which exhibits to be earlier than those of TA/BA by over 120 s. Meanwhile, the fire growth rate index (FGRA) of HA is higher than those of TA/BA by more than 15 %, implying that hydrothermal ageing dramatically increases the asphalt's fire risk. (3) Herein, the combustion mechanism of bimodal HRR peaks found for HA is analyzed, attributing to thin-layer boilover effect. This could be supported by plentiful pores observed on the combusted HA via scanning electron microscopy technique.

A dicentric chromosome is an abnormal chromosome with two centromeres on the same chromosome. It has been reported that dicentric chromosomes are specific biomarkers of radiation exposure, but dicentric chromosomes are rarely identified in newborns with multiple congenital anomalies. At 16 weeks of gestation, a 39-year-old pregnant woman (gravida 2, para 1) was referred to the prenatal diagnosis center for genetic counseling. The fetal ultrasonography indicated multiple anomalies. Subsequently, amniocentesis was performed, and the G-banding karyotype analysis showed a rare type of mosaicism. The C-banding karyotype analysis indicated a pseudo-dicentric chromosome X [psu dic (X; 18) (p11.2; p11.2)]. A single-nucleotide polymorphism array (SNP array) revealed three pathogenic copy number variations (CNVs). After genetic counseling, the parents chose to terminate this pregnancy. This study provides new evidence for a better understanding of the diagnosis of dicentric chromosomes and emphasizes on the importance of genetic counseling.

Abstract Metamaterials hold significant promise for enhancing the imaging capabilities of magnetic resonance imaging (MRI) machines as an additive technology, due to their unique ability to enhance local magnetic fields. However, despite their potential, the metamaterials reported in the context of MRI applications have often been impractical. This impracticality arises from their predominantly flat configurations and their susceptibility to shifts in resonance frequencies, preventing them from realizing their optimal performance. Here, a computational method for designing wearable and tunable metamaterials via freeform auxetics is introduced. The proposed computational‐design tools yield an approach to solving the complex circle packing problems in an interactive and efficient manner, thus facilitating the development of deployable metamaterials configured in freeform shapes. With such tools, the developed metamaterials may readily conform to a patient's knee, ankle, head, or any part of the body in need of imaging, and while ensuring an optimal resonance frequency, thereby paving the way for the widespread adoption of metamaterials in clinical MRI applications.

IntroductionLiuweizhiji Gegen-Sangshen beverage (LGS) is popular in China, which has been used for alleviating alcohol-mediated discomfort and preventing alcoholic liver disease (ALD). This beverage is consisted of six herbal components that are known as functional foods and fruits. LGS is rich in polysaccharides, however, the activity and quality evaluation of LGS-derived polysaccharides remain unexplored. The purpose of this study is thus to establish a comprehensive quality control methodology for the assessment of LGS polysaccharides (LGSP) and to further explore the anti-oxidant, anti-inflammatory as well as prebiotic effect of LGSP.MethodsLGSP was extracted, followed by analysis of molecular weight distribution, monosaccharide content and structural characterization via integrating the application of high-performance size exclusion chromatography (HPSEC), 1-phenyl-3-methyl-5-pyrazolone-HPLC (PMP-HPLC), fourier transform infrared spectroscopy (FT-IR) as well as nuclear magnetic resonance spectroscopy (NMR) techniques. The anti-oxidation activity of LGSP was determined by DPPH, ABTS, hydroxyl radical scavenging capacity and total antioxidant capacity. The anti-inflammation of LGSP were assessed on the RAW 264.7 cells. The effect of LGSP on growth of Lactobacillus, Bifidobacterium bifidum and Bifidobacterium adolescentis was evaluated.ResultsThe results demonstrated that LGSP had two molecular weight distribution peaks, with the average molecular weights of (6.569 ± 0.12) × 104 Da and (4.641 ± 0.30) × 104 Da. LGSP was composed of 8 monosaccharides, with galacturonic acid, glucose rhamnose and galactose representing the highest molar ratios. Homogalacturonic acid (HG) type and rhamnosegalacturonic acid glycans I (RG-I) type and α-1,4-glucan were present in LGSP. LGSP concentration in LGS was 17.94 ± 0.28 mg/mL. Furthermore, fingerprint analysis combined with composition quantification of 10 batches of LGSP demonstrated that there was a high similarity among batches. Notably, LGSP exhibited anti-oxidant effect and inhibited expressions of pro-inflammatory factors (TNF-α and IL-6) in LPS-stimulated RAW 264.7 cells. In addition, LGSP remarkably promoted the proliferation of probiotics Lactobacillus, Bifidobacterium bifidum and Bifidobacterium adolescentis, showing good prebiotic activity.DiscussionThe results of present study would be of help to gain the understanding of structure–activity relationship of LGSP, provide a reference for quality evaluation of bioactive LGSP, and facilitate development of unique health and functional products in the future.

Abstract Background Long non-coding RNAs (lncRNAs) are key regulators of the 6-methyladenosine (m6A) epigenetic modification, playing a role in the initiation and progression of tumors. However, the regulatory mechanisms in head and neck squamous cell carcinoma (HNSCC) remain elusive. In this study, we investigated the molecular regulatory mechanisms of the lncRNA RASAL2-AS1 in the occurrence and development of HNSCC tumors. Methods A bioinformatics analysis was conducted to analyze the expression level of RASAL2-AS1 in HNSCC and normal tissues. RASAL2-AS1 mRNA and protein levels were detected using RT-PCR and Western blotting. Wound healing, transwell assays, flow cytometry, M6A dot blot, and RNA immunoprecipitation experiments were conducted to explore the regulatory role of the RASAL2-AS1 and downstream targets METTL14/LIS1 signaling pathway in HNSCC. Immunohistochemical examination was conducted to evaluate the expression of METTL14 and LIS1 in HNSCC and normal tissues. A tumor xenograft model of BALB/c nude mice was established to assess the impact of RASAL2-AS1 on cell proliferation and growth. Results RASAL2-AS1 high expression in HNSCC and cells deteriorated with survival rates of HNSCC. RASAL2-AS1 overexpression in HNSCC accelerated cell migration, colony formation, cell proliferation, cell cycle in S stage, while RASAL2-AS1 knockdown in HNSC cells inhibited cell cycle in G1 stage. After silencing METTL14, the above effects induced by overexpression of the RASAL2-AS1 were reversed. RASAL2-AS1 overexpression prompted LIS1 expression, whereas RASAL2-AS1 silencing reduced LIS1 levels in HNSCC cells, which was confirmed by immunohistological staining. Results demonstrated elevated expression of METTL14 or LIS1 in tongue cancer tissues. Overexpression of RASAL2-AS1 promoted tumor weight and tumor volume, which was counteracted by pcDNA3.1 RASAL2-AS1 plus silencing METTL14 and METTL14 and LIS1 were significantly decreased. Conclusion Our study highlights the functional importance of the LncRNA RASAL2-AS1 in HNSCC and might assist in the development of a prognostic stratification and therapeutic approach. Which regulates HNSCC with the dependence of m6a manner.

Abstract We construct a two-tensor model with order-3 and present its W-representation. Moreover we derive the compact expressions of correlators from the W-representation and analyze the free energy in large N limit. In addition, we establish the correspondence between two colored Dyck walks in the Fredkin spin chain and tree operators in the ring. Based on the classification Dyck walks, we give the number of tree operators with the given level. Furthermore, we show the entanglement scaling of Fredkin spin chain beyond logarithmic scaling in the ordinary critical systems from the viewpoint of tensor model.

Abstract Purpose This study aimed to systematically compare the efficacy and safety of arthroscopic wafer procedure (AWP) versus ulnar shortening osteotomy (USO) for ulnar impaction syndrome (UIS) treatment. Methods All the studies included in this meta-analysis compared the efficacy of AWP to USO for UIS and were acquired through a comprehensive search across multiple databases. The meta-analysis was performed by calculating the effect sizes with the Cochrane Collaboration’s RevMan 5.4 software. Results A total of 8 articles were included in this analysis, comprising 148 cases in the AWP group and 163 cases in the USO group. The pooled estimates indicated no significant differences in combined Darrow’s Criteria or Modified Mayo Wrist Score, Modified Mayo Wrist Score, DASH scores, grip strength, VAS score, and postoperative ulnar variation. On the other hand, the patients in the AWP group exhibited fewer complications (OR = 0.17, 95%CI 0.05–0.54, P = 0.003) and a lower reoperation rate (OR = 0.12, 95%CI 0.05–0.28, P

Beyond 5G and 6G technologies mark a pivotal evolution in the telecommunications landscape. They are not only anticipated to deliver unprecedented speed and capacity but also to serve as critical enablers for a host of emerging technologies and applications, including joint communications and sensing, autonomous driving, augmented reality (AR), virtual reality (VR), and the burgeoning Internet of Things (IoT) [1], [2], [3]. As we transition into this new era, the expectations for system performance, reliability, and versatility are higher than ever before.

ABSTRACTThe growing number of Mpox cases in China has posed a challenge to public health. The prevalence of men who have sex with men behaviors among students has been consistently increasing each year in China, accompanied by a high frequency of unprotected anal sex. As crowded places, schools are highly likely to cause an Mpox outbreak among students through long-term close contact. Understanding university students’ perceptions about Mpox and willingness to vaccinate play a vital role in implementing preventive measures in schools. This study aimed to assess knowledge, concerns, and vaccine acceptance toward Mpox among university students in North and Northeast China. A cross-sectional study was conducted among 3831 university students from seven universities in North and Northeast China between September 10 and September 25, 2023. This study found a relative insufficiency in Mpox knowledge among university students (71.60%), with less than half expressing concern about the Mpox outbreak (39.57%), and the majority exhibiting a positive attitude to vaccination (76.30%). Multivariate regression analysis revealed that a good knowledge level was associated with age, study discipline, education level, and a high level of concern about Mpox. Male, elderly, or highly educated participants had a low level of concern about Mpox. Participants with a high level of knowledge toward Mpox were more likely to have the vaccination willingness. This study might help governments and schools to understand students’ Mpox perceptions and vaccination intentions, enabling them to implement effective measures in addressing the issue of inadequate understanding regarding Mpox among university students.

Silver–Russell syndrome (SRS) is a clinically and genetically heterogeneous disorder. A retrospective analysis predicted that the live birth prevalence of SRS in Estonia is 1:15,886 [Yakoreva et al., Eur J Hum Genet, 2019, 27(11), 1649–1658]. The most common causative genetic mechanism in the proband is loss of paternal methylation in the imprinted control region 1 (ICR1) at 11p15.5 chromosome. A few studies suggested that inherited or de novo loss-of-function alterations of the PLAG1 gene, including the whole-gene deletion and intragenic pathogenic variants, could cause a rare type of SRS. To date, less than 20 unrelated PLAG1-related SRS cases have been reported, and the clinical information about these cases is limited. We report the first prenatal case of SRS with 8q12 deletion (including the PLAG1 gene). The fetus presented with intrauterine growth retardation, small for gestational age, relative macrocephaly at birth, and a protruding forehead. Unlike classical SRS cases, the fetus had micrognathia and did not show body asymmetry. We hope that the literature review in this study provides new insights into genotype–phenotype relationships of PLAG1-related SRS.

Backgrounds Glioma is a highly aggressive type of brain tumor, and its prognosis is still poor despite recent progress in treatment strategies. G protein-coupled receptor 27 (GPR27) is a member of the G protein-coupled receptor family and has been reported to be involved in various cellular processes, including tumor progression. Nevertheless, the clinical potential and tumor-related role of GPR27 in glioma remain unknown. Here we aimed to explore the function and role of GPR27 in gliomas. Methods In the current study, we evaluated the expression and clinical significance of GPR27 in gliomas using data from The Cancer Genome Atlas (TCGA) datasets. We also conducted cellular experiments to evaluate the functional role of GPR27 in glioma cell growth. Results We found that GPR27 expression level was closely associated with disease status of glioma. Of note, GPR27 was negatively correlated with WHO grade, with grade IV samples showing the lowest GPR27 levels, while grade II samples showed the highest levels. Patients with IDH mutation or 1p/19q co-deletion exhibited higher GPR27 levels. In addition, lower GPR27 levels were correlated with higher death possibilities. In cellular experiments, we confirmed that GPR27 inhibited glioma cell growth. Conclusions Our results indicate that GPR27 may function as a potential prognostic biomarker and therapeutic target in gliomas. Further studies are needed to illustrate the signaling mechanism and clinical implications of GPR27 in gliomas.

Abstract Background Malignant melanoma is a highly heterogeneous skin cancer with the highest mortality rate among dermatological cancers. Catenins form functional networks in the nucleus to regulate gene expression and determine cell fate. Dysregulation of catenin expression correlates with the malignant characteristics of the tumor. We aimed to investigate the regulatory mechanisms of catenins in melanoma and to further define the function of catenin-related molecular signaling in the tumor microenvironment. Methods In this study, a bioinformatics approach combined with experimental validation was used to explore the potential tumor biology mechanisms of catenin-related signaling. Results Melanoma patients can be divided into two catenin clusters. Patients defined by high Junction Plakoglobin (JUP), Plakophilin 1 (PKP1), Plakophilin 3 (PKP3) levels (C2) had shorter survival time than other patients (C1). We demonstrated that JUP regulates Anterior Gradient 2 (AGR2)/LY6/PLAUR Domain Containing 3 (LYPD3) to maintain melanoma stemness and promotes glycolysis. We also found that LYPD3 was co-expressed with S100A9 and associated with immunosuppressive tumor microenvironment (TME). Conclusion The JUP/AGR2/LYPD3 signaling axis plays an important role in the malignant features of melanoma. Targeting the JUP/AGR2/LYPD3 signaling axis can help develop promising drugs.

Abstract Background Laparoscopic access to liver segment 7 (S7) is difficult for deep surgical situations and bleeding control. Herein, our proposed laparoscopic technique for S7 lesions using a self-designed tube method is introduced. Methods Clinical data of patients who underwent laparoscopic anatomical liver resection of S7 (LALR-S7) with the help of our self-designed tube to improve the exposure of S7 and bleeding control in the Second Affiliated Hospital, Third Military Medical University (Army Medical University) from April 2019 to December 2021 were retrospectively analyzed to evaluate feasibility and safety. Results Nineteen patients were retrospectively reviewed. The mean age was 51.3 ± 10.3 years; mean operation time, 194.5 ± 22.7 min; median blood loss, 160.0 ml (150.0–205.0 ml); and median length of hospital stay, 8.0 days (7.0–9.0 days). There was no case conversion to open surgery. Postoperative pathology revealed all cases of hepatocellular carcinoma (HCC). Free surgical margins were achieved in all patients. No major postoperative complications were observed. Patients with postoperative complications recovered after conservative treatment. During outpatient follow-up examination, no other abnormality was presented. All patients survived without tumor recurrence. Conclusions The preliminary clinical effect of our method was safe, reproducible and effective for LALR-S7. Further research is needed due to some limitations of this study.

Objective Palaeomagnetic study of the early Palaeozoic North Qiangtang Terrane can quantitatively constrain its palaeogeographic location since the Palaeozoic, which serves as an important foundation and key constraint for studying of the palaeotectonic pattern of the Qinghai-Tibet Plateau, the evolution of the Phanerozoic Tethys and palaeogeographic reconstruction. Methods Prior to palaeomagnetic study, it is essential to investigate the magnetic properties of rocks to identify the types and assemblages of magnetic carriers present in rocks, which will facilitate the selection of demagnetization experimental protocols and discussions on the primary nature of remanent magnetization. This paper focuses on the magnetic properties of limestone and sandstone from the Late Silurian Longmu Co Upper Formation in the North Qiangtang Terrane, through analyses of isothermal remanence acquisition curves, magnetic susceptibility with temperature(χ-T) curves, triaxial thermal demagnetization experiments, low-temperature magnetic properties tests, scanning electron microscopy (SEM) and energy dispersive spectral (EDS) analysis. Results Results show that magnetite is the predominant magnetic carrier in the limestone samples of the Longmu Co Upper Fm, with small amounts of pyrrhotite. The magnetic assemblage in the sandstone samples is more complex, dominated by magnetite, possibly with other magnetic minerals such as pyrrhotite. Conclusion Moreover, it demonstrates that stable high-temperature remanent magnetization components can be isolated from the limestone samples in the Longmu Co Upper Formation, making them suitable for further palaeomagnetic researches.

Ultrashort pulse transmission has been recognized as a primary problem that fundamentally hinders the development of ultrafast electronics beyond the current nanosecond timescale. This requires a transmission line or waveguide that exhibits an all-pass frequency behavior for the transmitted ultrashort pulse signals. However, this type of waveguiding structure has not yet been practically developed; groundbreaking innovations and advances in signal transmission technology are urgently required to address this scenario. Herein, we present a synthesized all-pass waveguide that demonstrates record guided-wave controlling capabilities, including eigenmode reshaping, polarization rotation, loss reduction, and dispersion improvement. We experimentally developed two waveguides for use in ultrabroad frequency ranges (direct current (DC)-to-millimeter-wave and DC-to-terahertz). Our results suggest that the waveguides can efficiently transmit picosecond electrical pulses while maintaining signal integrity. This waveguide technology is an important breakthrough in the evolution of ultrafast electronics, providing a path towards frequency-engineered ultrashort pulses for low-loss and low-dispersion transmissions.

The residual oil saturation of the matrix near the well zone of a tight reservoir is high due to the tight reservoir’s complex conditions, such as the small pore throat radius and low permeability of the matrix and the development of microfractures, which can result in serious water channeling, even after long-term water injection development. The aim of this paper is to improve the effects of depressurization and augmented injection for tight reservoir waterflooding development by reducing the tight matrix’s residual oil saturation, increasing and maintaining its water phase permeability near the well zone using a nano-SiO2 microemulsion system with a small particle size and high interfacial activity. Therefore, four nano-microemulsion systems were evaluated and screened for their temperature resistance, salt resistance, interfacial tension, solubilization, and dilution resistance. A microemulsion system of 13% A + 4% B + 4% C + 4% n-butanol + 6% oil phase + 69% NaCl solution (10%) + 1% OP-5 + 0.5% anti-temperature agent + 0.3% nanosilica material was preferred. According to the core displacement experiment, the depressurization rate can reach 28~60% when the injection concentration of the system is 1~10% and the injection volume is 2~5 PV. The results of the on-site test show that the water injection pressure dropped to 17.5 MPa, which was lower than the reservoir fracture re-opening pressure. The pressure reduction rate was approximately 20%. The validity period of the depressurization and augmented injection has reached 23 months to date.

Abstract Background Inflammatory, immune, and nutritional status are key factors in obstructive colorectal cancer (OCRC). This study aims to investigate the value of modified Naples prognostic score (M-NPS) in evaluating OCRC prognosis. Methods A total of 196 OCRC patients were retrospectively analyzed to construct M-NPS based on serum albumin (ALB), total cholesterol (CHOL), neutrophil:lymphocyte ratio (NLR), and lymphocyte:monocyte ratio (LMR), and then they were divided into three groups. The Kaplan–Meier (KM) method and Cox proportional hazard regression analysis were performed for overall survival (OS) and disease-free survival (DFS) of OCRC patients. Results Patients with high M-NPS had worse OS and DFS (P = 0.0001, P = 0.0011). Multivariate COX analysis showed that M-NPS was an independent prognostic factor for OCRC patients. Patients in the M-NPS 2 group had significantly worse OS (hazard ratio [HR] = 4.930 (95% confidence interval [95% CI], 2.217–10.964), P

When unbalanced faults such as single-phase voltage drop occur in the power grid, VSC-HVDC will fail or become unstable due to sudden changes in amplitude, phase, frequency, etc. of the power grid voltage. Therefore, the role of PLL in a non-ideal power grid is particularly important, but often PLL needs to make a compromise between dynamic response and system stability. Therefore, this paper proposes a free-PLL control strategy under non ideal power grid. First, according to the power transmission model of VSC-HVDC under power grid imbalance, the control command required by the inverter is given, which is determined in the environment free-PLL. Therefore, PLL is not required to retrace the phase of the grid voltage. In addition, in order to overcome the impact of the sudden change of power grid frequency on the free-PLL strategy, a frequency locking strategy is proposed in the free-PLL environment, the response time is about 1 grid cycle. Finally, in order to verify the effectiveness and correctness of the proposed strategy, the VSC-HVDC system’s active power transmission stability is guaranteed when the grid voltage encounters different degrees of single-phase sags.

High-resolution and thermal infrared hyperspectral data acquired from the Thermal Infrared Airborne Spectrographic Imager (TASI) have been recognized as efficient tools in geology, demonstrating significant potential for rock discernment. Deep learning (DL), as an advanced technology, has driven substantial advancements in lithological mapping by automatically extracting high-level semantic features from images to enhance recognition accuracy. However, gathering sufficient high-quality lithological samples for model training is challenging in many scenarios, posing limitations for data-driven DL approaches. Moreover, existing sample collection approaches are plagued by limited verifiability, subjective bias, and variation in the spectra of the same class at different locations. To tackle these challenges, a novel sample generation method called multi-lithology spectra sample selection (MLS3) is first employed. This method involves multiple steps: multiple spectra extraction, spectra combination and optimization, lithological type identification, and sample selection. In this study, the TASI hyperspectral data collected from the Liuyuan area in Gansu Province, China, were used as experimental data. Samples generated based on MLS3 were fed into five typical DL models, including two-dimensional convolutional neural network (2D-CNN), hybrid spectral CNN (HybridSN), multiscale residual network (MSRN), spectral-spatial residual network (SSRN), and spectral partitioning residual network (SPRN) for lithological mapping. Among these models, the accuracy of the SPRN reaches 84.03%, outperforming the other algorithms. Furthermore, MLS3 demonstrates superior performance, achieving an overall accuracy of 2.25–6.96% higher than other sample collection methods when SPRN is used as the DL framework. In general, MLS3 enables both the quantity and quality of samples, providing inspiration for the application of DL to hyperspectral lithological mapping.

Poplar (Populus L.) anthracnose is an infectious disease that seriously affects the growth and yields of poplar trees, and large-scale poplar infections have led to huge economic losses in the Chinese poplar industry. To efficiently and accurately detect poplar anthracnose for improved prevention and control, this study collected hyperspectral data from the leaves of four types of poplar trees, namely healthy trees and those with black spot disease, early-stage anthracnose, and late-stage anthracnose, and constructed a poplar anthracnose detection model based on machine learning and deep learning. We then comprehensively analyzed poplar anthracnose using advanced hyperspectral-based plant disease detection methodologies. Our research focused on establishing a detection model for poplar anthracnose based on small samples, employing the Design of Experiments (DoE)-based entropy weight method to obtain the best preprocessing combination to improve the detection model’s overall performance. We also analyzed the spectral characteristics of poplar anthracnose by comparing typical feature extraction methods (principal component analysis (PCA), variable combination population analysis (VCPA), and the successive projection algorithm (SPA)) with the vegetation index (VI) method (spectral disease indices (SDIs)) for data dimensionality reduction. The results showed notable improvements in the SDI-based model, which achieved 89.86% accuracy. However, this was inferior to the model based on typical feature extraction methods. Nevertheless, it achieved 100% accuracy for early-stage anthracnose and black spot disease in a controlled environment respectively. We conclude that the SDI-based model is suitable for low-cost detection tasks and is the best poplar anthracnose detection model. These findings contribute to the timely detection of poplar growth and will greatly facilitate the forestry sector’s development.

The sea cucumber is an essential nutrient source and a significant economic marine resource associated with successful aquaculture. However, sea cucumbers are highly susceptible to autolysis induced by endogenous protease after postmortem, and the phenomenon of body wall “melting” occurs, which seriously affects the food quality of products and the degree of acceptance by consumers. To satisfy the growing demand for fresh or processed sea cucumbers, we must clarify the autolysis mechanism of sea cucumbers and the methods to achieve autolysis regulation. In this paper, the factors leading to the quality deterioration and texture softening of sea cucumbers are reviewed, with emphasis on enzymatic characteristics, the autolysis mechanism, the effects of autolysis on the physicochemical properties of the body wall of the sea cucumber, and the development of potential natural protease inhibitors. We aim to provide some reference in future preservation and processing processes for sea cucumbers, promote new processing and preservation technologies, and advance the sea cucumber industry’s development.

Coral reef bleaching events have become more frequent all over the world and pose a serious threat to coral reef ecosystems. Therefore, there is an urgent need for better detection of coral reef bleaching in a time- and cost-saving manner. In recent years, remote sensing technology has often been utilized and gained recognition for coral reef bleaching detection. However, bleaching corals in the water always have weak spectral change signals, causing difficulties in using remote sensing data. Additionally, uneven change samples make it challenging to adequately capture the details of coral reef bleaching detection and produce thematic maps. To resolve these problems, a novel method named coral reef bleaching detection by positive-unlabeled bagging (CBD-PUB) is proposed in this paper. To test the capacity of the method, a series of multi-temporal Sentinel-2 remote sensing images are utilized, and Lizard Island in Australia is taken as a case study area. The pseudo-invariant feature atmospheric correction (PIF) algorithm is adopted to improve coral reef bleaching spectral signals. After that, CBD-PUB is employed to effectively explore coral reef bleaching variation and its corresponding influence relations. The experimental results show that the overall accuracy of bleaching detection by the proposed algorithm reaches 92.1% and outperforms the traditional method. It fully demonstrates the feasibility of the model for the field of coral reef bleaching detection and provides assistance in the monitoring and protection of coral environments.

Osteosarcoma (OS) is a highly aggressive and lethal malignant bone tumor that primarily afflicts children, adolescents, and young adults. However, the molecular mechanisms underlying OS pathogenesis remain obscure. Mounting evidence implicates dysregulated long non-coding RNAs (lncRNAs) in tumorigenesis and progression. These lncRNAs play a pivotal role in modulating gene expression at diverse epigenetic, transcriptional, and post-transcriptional levels. Uncovering the roles of aberrant lncRNAs would provide new insights into OS pathogenesis and novel tools for its early diagnosis and treatment. In this review, we summarize the significance of lncRNAs in controlling signaling pathways implicated in OS development, including the Wnt/β-catenin, PI3K/AKT/mTOR, NF-κB, Notch, Hippo, and HIF-1α. Moreover, we discuss the multifaceted contributions of lncRNAs to drug resistance in OS, as well as their potential to serve as biomarkers and therapeutic targets. This review aims to encourage further research into lncRNA field and the development of more effective therapeutic strategies for patients with OS.

Background: Mpox, one of the most serious threats to global health, is now being seen in small but rising numbers in Beijing, China. Our study aimed to investigate healthcare workers’ (HCWs) knowledge of Mpox and to explore reasons associated with their hesitancy to vaccinate against Mpox in Beijing, China. Methods: A cross-sectional study was conducted among HCWs in Beijing from July 24 to August 2, 2023, through an online questionnaire. Participants answered questions about sociodemographic characteristics, Mpox information sources, Mpox knowledge, perception of vaccines, and attitudes toward Mpox vaccination. We used Chi-squared test to compare difference in Mpox vaccination hesitancy between different groups. Multivariable logistic regression models were applied to analyze correlates of vaccination hesitancy among HCWs. Results: A total of 2331 HCWs completed the questionnaire, with an effective response rate of 92.45 % (2155/2331). Most of the HCWs in this study worked at tertiary hospitals (89.65 %), with a mean age of 36.69 ± 9.08 years. Among the 2155 participants, 52.99 % had over ten years of working experience, and 16.66 % were from high-risk departments relevant to Mpox treatment. Approximately 84.41 % knew about Mpox before this study, 80.79 % exhibited a high level of knowledge about Mpox, whereas 42.37 % were hesitant to be vaccinated against Mpox. Moreover, the hesitancy rate of HCWs in high-risk departments (47.91 %) was higher than in lower-risk departments (41.26 %). Higher educational level (aOR = 1.75, 95 %CI: 1.17–2.62), longer working years (1.71, 1.32–2.22), working at high-risk departments (1.34, 1.05–1.71), and lower level of knowledge about Mpox (1.78, 1.13–2.85) appeared as the most significant determinants of Mpox vaccination hesitancy among HCWs who knew about Mpox. For the HCWs who did not know about Mpox, longer working years (1.96, 1.02–3.78) were significant factors associated with their hesitancy. The predominant reason for hesitancy toward Mpox vaccination among HCWs encompassed apprehensions about vaccine side effects. Conclusion: HCWs had good knowledge of Mpox, whereas their Mpox vaccination hesitancy was also relatively high in Beijing, China. Increasing HCWs’ vaccination confidence and knowledge level about Mpox, especially for those working in high-risk departments, may be an essential way of reducing their hesitancy.

We present higher Chern-Simons-Antoniadis-Savvidy (ChSAS) forms based on crossed modules. We start from introducing a generalized multilinear symmetric invariant polynomial for the differential crossed modules and constructing a metric independent, higher gauge invariant, and closed form using the higher curvature forms. Then, we establish the higher Chern-Weil theorem and prove that the higher ChSAS forms are a special case of this theorem. Finally, we get the link of two independent higher ChSAS theories.

Abstract In social networks, bursts of activity often result from the imitative behavior between interacting agents. The Ising model, along with its variants in the social sciences, serves as a foundational framework to explain these phenomena through its critical properties. We propose an alternative generic mechanism for the emergence of collective exuberance within a broad class of agent-based models. We show that our model does not require the fine-tuning to a critical point, as is commonly done to explain bursts of activity using the Ising model and its variants. Instead, our approach hinges on the intrinsic non-symmetric and hierarchical organization of socio-economic networks. These non-normal networks exhibit transient and unsustainable surges in herd behavior across a wide range of control parameters even in the subcritical regime, thereby eliminating the need for the - arguably artificial - fine-tuning proximity to a critical point. To empirically validate our framework, we examine the behavior of meme stocks and establish a direct linkage between the size of financial bubbles and the degree of non-normality in the network, as quantified by the Kreiss constant. Our proposed mechanism presents an alternative that is more general than prevailing conceptions of instabilities in diverse social systems.

The present work studies the influence of oil pipe joint passing through annular blowout preventer (BOP) on its sealing performance in high pressure gas wells under snubbing service. When the oil pipe joint passes through the BOP, due to the change of its structure, it is easy to cause the rubber core seal failure of the BOP, resulting in the leakage of toxic and harmful gas in the well, which seriously threatens the safety of the operators. Aiming at the problem of gas leakage caused by rubber core seal failure of annular BOP, based on the rubber large deformation theory and rubber core seal mechanism, a dynamic finite element model of rubber core-oil pipe joint is established, and the correctness of the model is verified by comparing the failure of rubber core on site; The results show that when the oil pipe joint passes through the BOP, a sealing buffer zone (SBZ) will be formed at the upper and lower shoulder of the joint, and the contact stress of the rubber core will decrease by 10 MPa–30 MPa; Because of the funnel effect of the rubber core, the damage of the rubber core caused by the running oil pipe joint of the BOP is greater than that caused by the lift oil pipe joint; When lifting oil pipe, the existence of SBZ is easy to cause gas leakage in the well; The optimized structure of oil pipe joint with small inclination and long shoulder can significantly reduce the influence of SBZ on the sealing performance of BOP. The research work in this paper is of great significance to improve the dynamic sealing performance of BOP.

Monkeypox (mpox) is a zoonotic disease caused by the mpox virus (MPXV) that has been primarily limited to Central and West African nations since its discovery. The recent spread of the West African lineage of MPXV in historically unaffected countries has raised concerns for global public health. Despite a significant decrease in global mpox cases, there is still a risk of a global resurgence. This study reports the first local case of mpox caused by an imported case in the Chinese mainland. Polymerase chain reaction (PCR) diagnosed the two cases, and the viral genomes were obtained by next-generation sequencing. Genomic analysis revealed that the two strains shared an identical genome sequence and belonged to the B.1.3 branch of the West African lineage, which is the first local case of mpox caused by an imported case in the Chinese mainland, highlighting the potential threat of mpox in China and the immediate need for adequate surveillance measures.

The gas sensitivity of platinum doped TiO2 (101) to two kinds on polyvinyl chloride decomposition production, CO and HCl, were explored based on density functional theory. The binding energy, adsorption distance, charge transfer, and density of states distribution were simulated and discussed. The results suggest that the O oriented mode is probably the best mode for the CO gas molecules adsorption on the Pt-TiO2 and H oriented mode for the HCl adsorption system. HCl and CO molecules obtain electrons from Pt doped TiO2 surface. At room temperature, the recovery time of CO and HCl is 1.9743 × 10-10, 1.2094 × 10-10 respectively. HCl is easier to dissociate from Pt doped TiO2 surface with a little lower energy barrier compared to CO. This work provides theoretical adsorption information of Pt-TiO2 as gas sensor material for HCl and CO detection in the application of online condition monitoring and defect diagnosis in the power cables.

Abstract We present higher Chern-Simons theories based on (2-)crossed modules. We start from the generalized differential forms in Generalized Differential Calculus and define the corresponding generalized connections which consist of higher connections. Then we establish the generalized Chern-Simons forms to get the higher Chern-Simons actions. Finally, we develop the higher second Chern forms and Chern-Weil theorems.

Libration and precession are different body forces that are ubiquitous in many rapidly rotating systems, particularly in geophysical and astrophysical flows. Libration is a modulation of the background rotation magnitude, whereas precession is a modulation of the background rotation direction. Assessing the consequences of these body forces in large-scale flows is challenging. The Ekman number, the ratio of the rotation time scale to the viscous time scale quantifying the rotation speed, is extremely small, leading to extremely thin and intense shear layers in the flows even when the amplitudes of the body forces are very small. We consider the consequences of libration and precession numerically in a geometrically simple container, a cube, which lends itself to very efficient, accurate, and robust numerical treatment, with the axis of rotation passing through opposite vertices, so that all walls of the cube are at oblique angles to the rotation axis. This results in the geometric focusing of inertial wavebeams reflecting off the walls, whereby the energy density of the wavebeams increases along with the magnitude of their wavevector. The nature of this focusing depends on the forcing frequency but not on the body force. In the inviscid setting, wavebeams form infinitesimally thin vortex sheets, and their energy density becomes unbounded upon focusing. We present linear inviscid ray tracing to set the scene for the focusing of wavebeams and then consider viscous problems at an Ekman number that is typical of current state-of-the-art laboratory experiments. We begin by considering the linear responses, which are comprised of focusing viscous shear layers, of which their details are mostly captured via ray tracing, and particular solutions accounting for the body forces. These have complicated spatio-temporal structures, which differ for libration and precession. Increasing the forcing amplitude from zero introduces nonlinear interactions, enhances the focusing effects via vortex tilting and stretching when the shear layers reflect at the walls, and also introduces temporal superharmonics and a mean flow. When the magnitude of the mean flow is within a few percent of the magnitude of the instantaneous flow, instabilities breaking the spatio-temporal symmetries set in. These are localized in the oscillatory boundary layers where the reflections are concentrated and introduce broadband dynamics in the boundary layers, with additional inertial wavebeams emitted into the interior. The details again depend on the specifics of the body forces.

With the development of hyperspectral sensors, the availability of hyperspectral images (HSIs) has increased significantly, prompting advancements in deep learning-based hyperspectral image classification (HSIC) methods. Recently, graph convolutional networks (GCNs) have been proposed to process graph-structured data in non-Euclidean domains, and have been used for HSIC. The superpixel segmentation should be implemented first in the GCN-based methods, however, it is difficult to manually select the optimal superpixel segmentation sizes to obtain the useful information for classification. To solve this problem, we constructed a HSIC model based on a multiscale feature search-based graph convolutional network (MFSGCN) in this study. Firstly, pixel-level features of HSIs are extracted sequentially using 3D asymmetric decomposition convolution and 2D convolution. Then, superpixel-level features at different scales are extracted using multilayer GCNs. Finally, the neural architecture search (NAS) method is used to automatically assign different weights to different scales of superpixel features. Thus, a more discriminative feature map is obtained for classification. Compared with other GCN-based networks, the MFSGCN network can automatically capture features and obtain higher classification accuracy. The proposed MFSGCN model was implemented on three commonly used HSI datasets and compared to some state-of-the-art methods. The results confirm that MFSGCN effectively improves accuracy.

Human papillomavirus (HPV) is a prevalent cause of mucosal and cutaneous infections and underlying conditions ranging from benign warts to anogenital and oropharyngeal cancers affecting both males and females, notably cervical cancer. Cervical cancer is the fourth leading cause of cancer deaths among women globally and is the most impactful in low- and middle-income countries (LMICs), where the costs of screening and licensed L1-based HPV vaccines pose significant barriers to comprehensive administration. Additionally, the licensed L1-based HPV vaccines fail to protect against all oncogenic HPV types. This study generated three independent lots of an L2-based target antigen (LBTA), which was engineered from conserved linear L2-protective epitopes (aa11–88) from five human alphapapillomavirus genotypes in E. coli under cGMP conditions and adjuvanted with aluminum phosphate. Vaccination of rabbits with LBTA generated high neutralizing antibody titers against all 17 HPV types tested, surpassing the nine types covered by Gardasil®9. Passive transfer of naïve mice with LBTA antiserum revealed its capacity to confer protection against vaginal challenge with all 17 αHPV types tested. LBTA shows stability at room temperature over >1 month. Standard in vitro and in vivo toxicology studies suggest a promising safety profile. These findings suggest LBTA’s promise as a next-generation vaccine with comprehensive coverage aimed at reducing the economic and healthcare burden of cervical and other HPV+ cancers in LMICs, and it has received regulatory approval for a first-in-human clinical study (NCT05672966).

In this study, a method for the active shape control for carbon fiber-reinforced polymer (CFRC) reflectors using piezoelectric lead zirconate titanate (PZT) actuators is proposed. According to this method, a finite element model considering higher transverse shear deformation with independent voltage degrees of freedom is formulated by the Hamilton principle. An optimal shape controller that minimizes the discrete root mean square (RMS) error of a reflecting surface is applied. Then, the optimal arrangements of the PZT actuators are determined by numerical optimization methods, which are developed by modifying the classical Genetic Algorithm, with both single and multi-objective optimizations being studied. In the single optimization formulation, the number of actuators is considered as a constraint, and the RMS error of the reflector is regarded as the optimizing target. A hybrid method that combines the gradient projection method with an adaptive Genetic Algorithm is proposed to solve this problem. In the multi-objective optimization, the residual RMS errors and power consumption of the actuators are considered as the optimization targets. Pareto optimal solutions are obtained by an improved multi-objective Genetic Algorithm. Numerical examples are provided to show the effectiveness of the proposed methods.

The interplay between the immune system and cancer underscores the central role of immunotherapy in cancer treatment. In this context, the innate immune system plays a critical role in preventing tumor invasion. Myeloid differentiation factor 88 (MyD88) is crucial for innate immunity, and activation of MyD88 promotes the production of inflammatory cytokines and induces infiltration, polarization, and immune escape of immune cells in the tumor microenvironment. Additionally, abnormal MyD88 signaling induces tumor cell proliferation and metastasis, which are closely associated with poor prognosis. Therefore, MyD88 could serve as a novel tumor biomarker and is a promising target for cancer therapy. Current strategies targeting MyD88 including inhibition of signaling pathways and protein multimerization, have made substantial progress, especially in inflammatory diseases and chronic inflammation-induced cancers. However, the specific role of MyD88 in regulating tumor immunity and tumorigenic mechanisms remains unclear. Therefore, this review describes the involvement of MyD88 in tumor immune escape and disease therapy. In addition, classical and non-classical MyD88 inhibitors were collated to provide insights into potential cancer treatment strategies. Despite several challenges and complexities, targeting MyD88 is a promising avenue for improving cancer treatment and has the potential to revolutionize patient outcomes.