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187 results on '"Ke, Guoliang"'

6. Aptamer-functionalized DNA Nanostructures for Biological Applications

Author

Fu, Xiaoyi, Peng, Fangqi, Lee, Jungyeon, Yang, Qi, Zhang, Fei, Xiong, Mengyi, Kong, Gezhi, Meng, Hong-min, Ke, Guoliang, Zhang, Xiao-Bing, Olivucci, Massimo, Editor-in-Chief, Wong, Wai-Yeung, Editor-in-Chief, Bayley, Hagan, Series Editor, Hughes, Greg, Series Editor, Hunter, Christopher A., Series Editor, Hwang, Seong-Ju, Series Editor, Ishihara, Kazuaki, Series Editor, Kirchner, Barbara, Series Editor, Krische, Michael J., Series Editor, Larsen, Delmar, Series Editor, Lehn, Jean-Marie, Series Editor, Luque, Rafael, Series Editor, Siegel, Jay S., Series Editor, Thiem, Joachim, Series Editor, Venturi, Margherita, Series Editor, Wong, Chi-Huey, Series Editor, Wong, Henry N.C., Series Editor, Yam, Vivian Wing-Wah, Series Editor, Yan, Chunhua, Series Editor, You, Shu-Li, Series Editor, Fan, Chunhai, editor, and Ke, Yonggang, editor

Published
2020
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8. Nucleic Acids Analysis

Author

Zhao, Yongxi, Zuo, Xiaolei, Li, Qian, Chen, Feng, Chen, Yan-Ru, Deng, Jinqi, Han, Da, Hao, Changlong, Huang, Fujian, Huang, Yanyi, Ke, Guoliang, Kuang, Hua, Li, Fan, Li, Jiang, Li, Min, Li, Na, Lin, Zhenyu, Liu, Dingbin, Liu, Juewen, Liu, Libing, Liu, Xiaoguo, Lu, Chunhua, Luo, Fang, Mao, Xiuhai, Sun, Jiashu, Tang, Bo, Wang, Fei, Wang, Jianbin, Wang, Lihua, Wang, Shu, Wu, Lingling, Wu, Zai-Sheng, Xia, Fan, Xu, Chuanlai, Yang, Yang, Yuan, Bi-Feng, Yuan, Quan, Zhang, Chao, Zhu, Zhi, Yang, Chaoyong, Zhang, Xiao-Bing, Yang, Huanghao, Tan, Weihong, and Fan, Chunhai

Published
2021
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9. Organic Fluorophores with Large Stokes Shift for Bioimaging and Biosensing.

Author

Liu, Hong, Jiang, Gangwei, Ke, Guoliang, Ren, Tian‐Bing, and Yuan, Lin

Subjects
STOKES shift, FLUOROPHORES, SIGNAL-to-noise ratio
Abstract

Fluorophores and fluorophores‐based probes or labels can visualize the structures and content fluctuations of biomolecules, and have made great progress in the broad range of biomedicine fields. However, many commercially available fluorophores suffer from small Stokes shift, which results in insufficient signal‐to‐noise ratio and self‐quenching in advanced imaging techniques. Moreover, the small Stokes shift also hinders the application of fluorophores in some complex imaging, such as single‐excitation multicolor imaging. In the past two decades, many effects have been made to enlarge Stokes shift of fluorophores. In this review, we clarified the reasons for the small Stokes shift building on the structural analysis of fluorophores, systematically summarize the methods of structural modification to increase Stokes shift, and present some representative applications of fluorophores with large Stokes shift in imaging and sensing. [ABSTRACT FROM AUTHOR]

Published
2024
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14. Endogenous Enzyme-Driven Amplified DNA Nanocage Probe for Selective and Sensitive Imaging of Mature MicroRNAs in Living Cancer Cells

Author

Gao, Yingying, Gong, Chaonan, Chen, Mei, Huan, Shuangyan, Zhang, Xiao-Bing, and Ke, Guoliang

Abstract

Selective and sensitive imaging of intracellular mature microRNAs (miRNAs) is of great importance for biological process study and medical diagnostics. However, this goal remains challenging because of the interference of precursor miRNAs (pre-miRNAs) and the low abundance of mature miRNAs. Herein, we develop an endogenous enzyme-driven amplified DNA nanocage probe (Acage) for the selective and sensitive imaging of mature miRNAs in living cells. The Acage consists of a microRNA-responsive probe, an endogenous enzyme-driven fuel strand, and a DNA nanocage framework with an inner cavity. Benefiting from the size selectivity of DNA nanocage, smaller mature miRNAs rather than larger pre-miRNAs are allowed to enter the cavity of DNA nanocage for molecular recognition; thus, Acage can significantly reduce the signal interference of pre-miRNAs. Moreover, with the driving force of an endogenous enzyme apurinic/apyrimidinic endonuclease 1 (APE1) for efficient signal amplification, Acage enables sensitive intracellular miRNA imaging without an additional external intervention. With these features, Acage was successfully applied for intracellular imaging of mature miRNAs during drug treatment. We believe that this strategy provides a promising pathway for better understanding the functions of mature microRNAs in biological processes and medical diagnostics.

Published
2024
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15. Chemical Approaches to Optimize the Properties of Organic Fluorophores for Imaging and Sensing.

Author

Jiang, Gangwei, Liu, Han, Liu, Hong, Ke, Guoliang, Ren, Tian‐Bing, Xiong, Bin, Zhang, Xiao‐Bing, and Yuan, Lin

Subjects
FLUOROPHORES, STOKES shift, PERMEABILITY
Abstract

Organic fluorophores are indispensable tools in cells, tissue and in vivo imaging, and have enabled much progress in the wide range of biological and biomedical fields. However, many available dyes suffer from insufficient performances, such as short absorption and emission wavelength, low brightness, poor stability, small Stokes shift, and unsuitable permeability, restricting their application in advanced imaging technology and complex imaging. Over the past two decades, many efforts have been made to improve these performances of fluorophores. Starting with the luminescence principle of fluorophores, this review clarifies the mechanisms of the insufficient performance for traditional fluorophores to a certain extent, systematically summarizes the modified approaches of optimizing properties, highlights the typical applications of the improved fluorophores in imaging and sensing, and indicates existing problems and challenges in this area. This progress not only proves the significance of improving fluorophores properties, but also provide a theoretical guidance for the development of high‐performance fluorophores. [ABSTRACT FROM AUTHOR]

Published
2024
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32. Nucleic Acids Analysis

Author

Zhao, Yongxi, primary, Zuo, Xiaolei, additional, Li, Qian, additional, Chen, Feng, additional, Chen, Yan-Ru, additional, Deng, Jinqi, additional, Han, Da, additional, Hao, Changlong, additional, Huang, Fujian, additional, Huang, Yanyi, additional, Ke, Guoliang, additional, Kuang, Hua, additional, Li, Fan, additional, Li, Jiang, additional, Li, Min, additional, Li, Na, additional, Lin, Zhenyu, additional, Liu, Dingbin, additional, Liu, Juewen, additional, Liu, Libing, additional, Liu, Xiaoguo, additional, Lu, Chunhua, additional, Luo, Fang, additional, Mao, Xiuhai, additional, Sun, Jiashu, additional, Tang, Bo, additional, Wang, Fei, additional, Wang, Jianbin, additional, Wang, Lihua, additional, Wang, Shu, additional, Wu, Lingling, additional, Wu, Zai-Sheng, additional, Xia, Fan, additional, Xu, Chuanlai, additional, Yang, Yang, additional, Yuan, Bi-Feng, additional, Yuan, Quan, additional, Zhang, Chao, additional, Zhu, Zhi, additional, Yang, Chaoyong, additional, Zhang, Xiao-Bing, additional, Yang, Huanghao, additional, Tan, Weihong, additional, and Fan, Chunhai, additional

Published
2020
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42. CRISPR‐Cas12a System for Biosensing and Gene Regulation.

Author

Shi, Yuyan, Fu, Xiaoyi, Yin, Yao, Peng, Fangqi, Yin, Xia, Ke, Guoliang, and Zhang, Xiaobing

Subjects
GENETIC regulation
Abstract

Clustered regularly interspaced short palindromic repeats (CRISPR) is a promising technology in the biological world. As one of the CRISPR‐associated (Cas) proteins, Cas12a is an RNA‐guided nuclease in the type V CRISPR‐Cas system, which has been a robust tool for gene editing. In addition, due to the discovery of target‐binding‐induced indiscriminate single‐stranded DNase activity of Cas12a, CRISPR‐Cas12a also exhibits great promise in biosensing. This minireview not only gives a brief introduction to the mechanism of CRISPR‐Cas12a but also highlights the recent developments and applications in biosensing and gene regulation. Finally, future prospects of the CRISPR‐Cas12a system are also discussed. We expect this minireview will inspire innovative work on the CRISPR‐Cas12a system by making full use of its features and advantages. [ABSTRACT FROM AUTHOR]

Published
2021
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46. Fluorescence Resonance Energy Transfer-Based DNA Nanoprism with a Split Aptamer for Adenosine Triphosphate Sensing in Living Cells

Author

Zheng, Xiaofang, Peng, Ruizi, Jiang, Xi, Wang, Yaya, Xu, Shuai, Ke, Guoliang, Fu, Ting, Liu, Qiaoling, Huan, Shuangyan, and Zhang, Xiaobing

Abstract

We have developed a DNA nanoprobe for adenosine triphosphate (ATP) sensing in living cells, based on the split aptamer and the DNA triangular prism (TP). In which nucleic acid aptamer was split into two fragments, the stem of the split aptamer was respectively labeled donor and acceptor fluorophores that underwent a fluorescence resonance energy transfer if two ATP molecules were bound as target molecule to the recognition module. Hence, ATP as a target induced the self-assembly of split aptamer fragments and thereby brought the dual fluorophores into close proximity for high fluorescence resonance energy transfer (FRET) efficiency. In the in vitro assay, an almost 5-fold increase in FA/FDsignal was observed, the fluorescence emission ratio was found to be linear with the concentration of ATP in the range of 0.03–2 mM, and the nanoprobe was highly selective toward ATP. For the strong protecting capability to nucleic acids from enzymatic cleavage and the excellent biocompatibility of the TP, the DNA TP nanoprobe exhibited high cellular permeability, fast response, and successfully realized “FRET-off” to “FRET-on” sensing of ATP in living cells. Moreover, the intracellular imaging experiments indicated that the DNA TP nanoprobe could effectively detect ATP and distinguish among changes of ATP levels in living cells. More importantly, using of the split aptamer and the FRET-off to FRET-on sensing mechanism could efficiently avoid false-positive signals. This design provided a strategy to develop biosensors based on the DNA nanostructures for intracellular molecules analysis.

Published
2024
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47. DNA-Mediated Morphological Control of Silver Nanoparticles

Author

Li, Jiuxing, primary, Zhu, Zhi, additional, Liu, Fang, additional, Zhu, Bingqing, additional, Ma, Yanli, additional, Yan, Jinmao, additional, Lin, Bingqian, additional, Ke, Guoliang, additional, Liu, Rudi, additional, Zhou, Leiji, additional, Tu, Song, additional, and Yang, Chaoyong, additional

Published
2016
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