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2. Scrapie-associated polymorphisms of the prion protein gene (PRNP) in Nigerian native goats

Author

Adeola, Adeniyi C., Bello, Semiu F., Abdussamad, Abdussamad M., Mark, Akanbi I., Sanke, Oscar J., Onoja, Anyebe B., Nneji, Lotanna M., Abdullahi, Nasiru, Olaogun, Sunday C., Rogo, Lawal D., Mangbon, Godwin F., Pedro, Shamsudeen L., Hiinan, Manasseh P., Mukhtar, Muhammad M., Ibrahim, Jebi, Saidu, Hayatu, Dawuda, Philip M., Bala, Rukayya K., Abdullahi, Hadiza L., Salako, Adebowale E., Kdidi, Samia, Yahyaoui, Mohamed Habib, and Yin, Ting-Ting

Published
2023
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3. Polymorphism of prion protein gene (PRNP) in Nigerian sheep

Author

Adeola, Adeniyi C., primary, Bello, Semiu F., additional, Abdussamad, Abdussamad M., additional, Mark, Akanbi I., additional, Sanke, Oscar J., additional, Onoja, Anyebe B., additional, Nneji, Lotanna M., additional, Abdullahi, Nasiru, additional, Olaogun, Sunday C., additional, Rogo, Lawal D., additional, Mangbon, Godwin F., additional, Pedro, Shamsudeen L., additional, Hiinan, Manasseh P., additional, Mukhtar, Muhammad M., additional, Ibrahim, Jebi, additional, Saidu, Hayatu, additional, Dawuda, Philip M., additional, Bala, Rukayya K., additional, Abdullahi, Hadiza L., additional, Salako, Adebowale E., additional, Kdidi, Samia, additional, Yahyaoui, Mohamed Habib, additional, and Yin, Ting-Ting, additional

Published
2023
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4. Genetic assignment of Tunisian meat sheep breeds and the effect of the reduction of microsatellites number on their structure assessment

Author

Kdidi, Samia, Calvo Lacosta, Jorge Hugo, Gaouar, Semir Bechir Suheil, Dbara, Mohamed, and (et al.)

Subjects
Microsatélites, Túnez, Razas (animales), Ovinos
Abstract

Microsatellite markers succeeded to reveal different population genetic parameters. The present work aimed to investigate the genetic assignment and structure of the Tunisian meat sheep breeds. (Barbarin (BB), Western Thin Tail (WTT), and Black Thibar (BT)).The current study also opted for testing different methods of assignment implemented in several programs for genetic identification and traceability purposes of these breeds and for assessing whether these markers could be useful for an efficient genetic assignment of these ovine breeds. The genotypes of 90 animals (30 samples per breed) were typed for 22 microsatellite markers. All the loci displayed a high polymorphic content (between 0.561 and 0.884). The GENECLASS2 and the WHICHLOCI programs were used to choose the most powerful markers (17 microsatellites). The FLOCK program was more efficient with 22 markers. Genetic differentiation tests (FST = 0.0127) and assignment of individuals to populations revealed the highest level of misassignment in BB and WTT breeds, while the BT breed revealed the highest percentage of individuals assigned to itself. The reduction of the number of microsatellites (from 22 to 17) does not affect the assessment of the genetic structure of Tunisian sheep breeds. This result shed the light on the importance of the shift towards lambs with thin tails imposed by the butchers. It also revealed the unfitness of microsatellite markers in genetic identification analysis for studied sheep breeds. Published

Published
2023

8. Analysis of differences in prion protein gene (<italic>PRNP</italic>) polymorphisms between Algerian and Southern Italy's goats.

Author

Fantazi, Khaled, Migliore, Sergio, Kdidi, Samia, Racinaro, Luca, Tefiel, Hakim, Boukhari, Rachid, Federico, Giovanni, Di Marco Lo Presti, Vincenzo, Gaouar, Semir Bechir Suheil, and Vitale, Maria

Subjects
PRIONS, GENETIC polymorphisms, RUMINANTS, GENOTYPES, GOATS
Abstract

Goats are adapted to live in harsh areas of the world ensuring minimum levels of milk and meat production without much economic input. Scrapie is a fatal prion disease of small ruminants detected in different countries worldwide. Polymorphisms in the prion protein gene (PRNP) modify the degree of susceptibility/resistance to scrapie. PRNP polymorphisms were analysed in the four main Algerian goat breeds (Naine de Kabylie, Arbia, Mozabite, Mekatia), and in two native breeds of South Italy (Aspromontana from Calabria and Cilentana from Campania). The results were compared also with two previously described Sicilian native breeds (Girgentana and Rossa Mediterranea). Seven amino acid substitutions were detected in Naine de Kabylie goat which represents the original native Berber breed. All other breeds presented no more than six variants and common polymorphisms were present at codons, 154 and 240. Isoleucine at 137 was present in Algerian breeds only. The Italian Cilentana shared more variants with Algerian breeds whereas the Aspromontana breed was the only one to show a Serine at 127. Exclusive new variants were not detected since all polymorphisms were already described in other goats worldwide. The scrapie protective allele encoding lysine (K) at codon 222 was detected in the Naine de Kabylie and M’zabite breeds at low-frequency whereas is present with frequencies higher than 10% in all the Italian breeds reared in regions with elevated scrapie incidence. The overall results showed a substantial number of polymorphisms in PRNP, particularly in Naine de Kabylie breed, which carried also unique genotypes. [ABSTRACT FROM AUTHOR]

Published
2018
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