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2 results on '"Kazuya Fujiyama"'

1. Cell Lysis Directed by SulA in Response to DNA Damage in Escherichia coli

Author

Masayuki Murata, Keiko Nakamura, Tomoyuki Kosaka, Natsuko Ota, Ayumi Osawa, Ryunosuke Muro, Kazuya Fujiyama, Taku Oshima, Hirotada Mori, Barry L. Wanner, and Mamoru Yamada

Subjects
DNA damage, SulA, cell lysis, SoxS, LpxC, Biology (General), QH301-705.5, Chemistry, QD1-999
Abstract

The SOS response is induced upon DNA damage and the inhibition of Z ring formation by the product of the sulA gene, which is one of the LexA-regulated genes, allows time for repair of damaged DNA. On the other hand, severely DNA-damaged cells are eliminated from cell populations. Overexpression of sulA leads to cell lysis, suggesting SulA eliminates cells with unrepaired damaged DNA. Transcriptome analysis revealed that overexpression of sulA leads to up-regulation of numerous genes, including soxS. Deletion of soxS markedly reduced the extent of cell lysis by sulA overexpression and soxS overexpression alone led to cell lysis. Further experiments on the SoxS regulon suggested that LpxC is a main player downstream from SoxS. These findings suggested the SulA-dependent cell lysis (SDCL) cascade as follows: SulA→SoxS→LpxC. Other tests showed that the SDCL cascade pathway does not overlap with the apoptosis-like and mazEF cell death pathways.

Published
2021
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2. Cell Lysis Directed by SulA in Response to DNA Damage in Escherichia coli

Author

Mamoru Yamada, Ryunosuke Muro, Hirotada Mori, Taku Oshima, Natsuko Ota, Tomoyuki Kosaka, Ayumi Osawa, Barry L. Wanner, Keiko Nakamura, Masayuki Murata, and Kazuya Fujiyama

Subjects
0301 basic medicine, Programmed cell death, endocrine system, Lysis, DNA damage, QH301-705.5, 030106 microbiology, Cell, Apoptosis, Article, Catalysis, LpxC, Amidohydrolases, Inorganic Chemistry, 03 medical and health sciences, chemistry.chemical_compound, Bacterial Proteins, Escherichia coli, medicine, SulA, SoxS, Physical and Theoretical Chemistry, SOS response, Biology (General), Molecular Biology, QD1-999, Spectroscopy, Chemistry, urogenital system, Escherichia coli Proteins, Serine Endopeptidases, Organic Chemistry, Gene Expression Regulation, Bacterial, General Medicine, Computer Science Applications, Cell biology, SOXS, 030104 developmental biology, Regulon, medicine.anatomical_structure, Genes, Bacterial, embryonic structures, Trans-Activators, cell lysis, Cell Division, DNA
Abstract

The SOS response is induced upon DNA damage and the inhibition of Z ring formation by the product of the sulA gene, which is one of the LexA-regulated genes, allows time for repair of damaged DNA. On the other hand, severely DNA-damaged cells are eliminated from cell populations. Overexpression of sulA leads to cell lysis, suggesting SulA eliminates cells with unrepaired damaged DNA. Transcriptome analysis revealed that overexpression of sulA leads to up-regulation of numerous genes, including soxS. Deletion of soxS markedly reduced the extent of cell lysis by sulA overexpression and soxS overexpression alone led to cell lysis. Further experiments on the SoxS regulon suggested that LpxC is a main player downstream from SoxS. These findings suggested the SulA-dependent cell lysis (SDCL) cascade as follows: SulA→SoxS→LpxC. Other tests showed that the SDCL cascade pathway does not overlap with the apoptosis-like and mazEF cell death pathways.

Published
2021

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