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Antibodies inhibiting the reverse transcriptase (RT) of human immunodeficiency virus type-1 (HIV-1) were found to be generated in the serum of mice repeatedly infected with a vaccinia virus recombinant, WRRT, expressing the enzyme. A monoclonal antibody (mAb), 7C4, which specifically and almost completely inhibits the RNA-dependent DNA polymerase activity of HIV-1 RT was produced from a mouse repeatedly immunized with WRRT. 7C4 seems to be specific for HIV-1 among retroviruses: 7C4 inhibited RT activity of three strains of HIV-1 (IIIB, Bru, and IMS-1) but not of two strains of HIV-2 (GH-1 and LAV-2) or two strains of SIV (MAC and MND). The immunoglobulin isotype of three, which includes 7C4, out of four mAbs produced from spleen cells of the immunized mouse were IgG2a. This immunization method that avoids protein denaturation may preferentially induce a T helper type-1 immune response and increase the chances of producing the only occasionally obtainable mAb capable of recognizing a conformational epitope and completely inhibiting enzyme activity.

High-performance liquid chromatography (HPLC) on newly developed spherical beads of hydroxyapatite (HAP) was applied to the separation of different molecular forms of mouse IgA and IgM monoclonal antibodies (mAbs). Monomeric, dimeric, trimeric and tetrameric forms of an IgA mAb were eluted from the column separately with appreciable differences in retention volume by a 20 ml (40 min) gradient of phosphate buffer (pH 6.8) of concentration from 10 to 400 mM. The volumes of respective forms were highly reproducible. In addition, a monomeric form was resolved successfully from a pentameric form of IgM mAbs by HPLC on HAP beads under the same conditions. All forms of IgA and IgM mAbs were eluted almost quantitatively from the column. The results indicate that HPLC on HAP beads is useful for isolation and characterization of different molecular forms of IgA and IgM mAbs.

Ein neues Indan-1-ol-Derivat aus den oberirdischen Teilen von Pteris wallichiana AGARDH. wurde als 1 (R), 2 (R), 3 (R)-1, 3-Dihydroxy-2, 5, 7-trimethyl-6-hydroxyathyl-indan-1-O-β-D-glukosid (I) und ein neues Indan-1-on-Derivat aus den oberirdischen Teilen von P. semipinnata L. als 3-Hydroxy-6-hydroxymethyl-2, 5, 7-trimethyl-indan-1-on (XII) identifiziert.