22 results on '"Kayla M. Williams"'
Search Results
2. 2015 Health Related Behaviors Survey: Mental and Emotional Health Among U.S. Active-Duty Service Members
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Charles C. Engel, Jennifer Hawes-Dawson, Robin L. Beckman, Molly Doyle, Rebecca L. Collins, Lisa Sontag-Padilla, Matthew Cefalu, Rajeev Ramchand, Kayla M. Williams, Sarah O. Meadows, and Amii M. Kress
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Military personnel ,medicine.medical_specialty ,Active duty ,Emotional health ,medicine ,Health related ,Service member ,Psychiatry ,Psychology ,Depression (differential diagnoses) ,Sexual assault - Abstract
The 2015 Health Related Behaviors Survey sought information on the health, health-related behaviors, and well-being of active-duty service members. This brief reviews results for mental and emotional health.
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- 2018
3. Are They Living Healthy? How Well Are Airmen Taking Care of Themselves
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Robin L. Beckman, Molly Waymouth, Rebecca L. Collins, Amii M. Kress, Sarah O. Meadows, Lisa Sontag-Padilla, Jennifer Hawes-Dawson, Matthew Cefalu, Rajeev Ramchand, Kayla M. Williams, and Charles C. Engel
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Gerontology ,Health related quality of life ,Military personnel ,Sexual behavior ,Infographic ,medicine ,Health related ,Service member ,Substance use ,medicine.disease ,Psychology ,Obesity - Abstract
The 2015 Health Related Behaviors Survey sought information on the health, health-related behaviors, and well-being of active-duty service members. This infographic reports key findings across the Air Force.
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- 2018
4. 2015 Health Related Behaviors Survey: Substance Use Among U.S. Active-Duty Service Members
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Rebecca L. Collins, Robin L. Beckman, Amii M. Kress, Kayla M. Williams, Lisa Sontag-Padilla, Molly Waymouth, Rajeev Ramchand, Sarah O. Meadows, Jennifer Hawes-Dawson, Charles C. Engel, and Matthew Cefalu
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Military personnel ,medicine.medical_specialty ,Active duty ,Family medicine ,medicine ,Health related ,Service member ,Substance use ,Psychology ,Prescription drug abuse - Abstract
The 2015 Health Related Behaviors Survey sought information on the health, health-related behaviors, and well-being of active-duty service members. This brief reviews results for substance use, particularly use of alcohol, tobacco, and medication.
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- 2018
5. 2015 Health Related Behaviors Survey: Summary Findings and Policy Implications
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Kayla M. Williams, Molly Waymouth, Charles C. Engel, Rebecca L. Collins, Rajeev Ramchand, Jennifer Hawes-Dawson, Robin L. Beckman, Sarah O. Meadows, Lisa Sontag-Padilla, Matthew Cefalu, and Amii M. Kress
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Health related quality of life ,medicine.medical_specialty ,Military personnel ,Sexual behavior ,medicine ,Health related ,Service member ,Substance use ,Psychiatry ,Psychology ,Mental health - Abstract
The 2015 Health Related Behaviors Survey sought information on the health, health-related behaviors, and well-being of active-duty service members. This brief summarizes results for broad topics of the survey, such as substance use and mental health.
- Published
- 2018
6. 2015 Health Related Behaviors Survey: Sexual Orientation, Transgender Identity, and Health Among U.S. Active-Duty Service Members
- Author
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Kayla M. Williams, Rebecca L. Collins, Robin L. Beckman, Lisa Sontag-Padilla, Amii M. Kress, Jennifer Hawes-Dawson, Molly Waymouth, Rajeev Ramchand, Sarah O. Meadows, Charles C. Engel, and Matthew Cefalu
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Health related quality of life ,Military personnel ,Active duty ,Transgender identity ,Sexual orientation ,Health related ,Service member ,Psychology ,Social psychology - Abstract
The 2015 Health Related Behaviors Survey sought information on the health, health-related behaviors, and well-being of active-duty service members. This brief reviews results for sexual orientation, transgender identity, and related health.
- Published
- 2018
7. 2015 Health Related Behaviors Survey: Physical Health and Functional Limitations Among U.S. Active-Duty Service Members
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Rebecca L. Collins, Kayla M. Williams, Jennifer Hawes-Dawson, Matthew Cefalu, Molly Waymouth, Charles C. Engel, Robin L. Beckman, Lisa Sontag-Padilla, Sarah O. Meadows, Rajeev Ramchand, and Amii M. Kress
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Military personnel ,Active duty ,Applied psychology ,Health related ,Physical health ,Service member ,Psychology - Abstract
The 2015 Health Related Behaviors Survey sought information on the health, health-related behaviors, and well-being of active-duty service members. This brief reviews results for physical health and functional limitations.
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- 2018
8. 2015 Health Related Behaviors Survey: Sexual Behavior and Health Among U.S. Active-Duty Service Members
- Author
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Robin L. Beckman, Amii M. Kress, Rajeev Ramchand, Kayla M. Williams, Sarah O. Meadows, Molly Waymouth, Jennifer Hawes-Dawson, Charles C. Engel, Rebecca L. Collins, Lisa Sontag-Padilla, and Matthew Cefalu
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Military personnel ,medicine.medical_specialty ,Active duty ,Sexual behavior ,Family medicine ,medicine ,Health related ,Service member ,Psychology - Abstract
The 2015 Health Related Behaviors Survey sought information on the health, health-related behaviors, and well-being of active-duty service members. This brief reviews results for sexual behavior and health.
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- 2018
9. 2015 Health Related Behaviors Survey: Health Promotion and Disease Prevention Among U.S. Active-Duty Service Members
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Robin L. Beckman, Sarah O. Meadows, Rajeev Ramchand, Kayla M. Williams, Jennifer Hawes-Dawson, Charles C. Engel, Lisa Sontag-Padilla, Matthew Cefalu, Amii M. Kress, Molly Waymouth, and Rebecca L. Collins
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medicine.medical_specialty ,Active duty ,business.industry ,Health related ,Physical exercise ,Service member ,medicine.disease ,Obesity ,Military personnel ,Health promotion ,Family medicine ,Medicine ,Disease prevention ,business - Abstract
The 2015 Health Related Behaviors Survey sought information on the health, health-related behaviors, and well-being of active-duty service members. This brief reviews results for health promotion and disease prevention.
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- 2018
10. Public-Private Partnerships for Providing Behavioral Health Care to Veterans and Their Families: What Do We Know, What Do We Need to Learn, and What Do We Need to Do?
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Eric R, Pedersen, Nicole K, Eberhart, Kayla M, Williams, Terri, Tanielian, Caroline, Batka, and Deborah M, Scharf
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humanities ,health care economics and organizations ,Military Health - Abstract
American veterans and their family members struggle with behavioral health problems, yet few engage in treatment to address these problems. Barriers to care include trouble accessing treatment and limited communication between civilian and military health care systems, which treat veterans and their family members separately. Even though the Department of Veterans Affairs (VA) is making efforts to address barriers to care, more work is needed to effectively serve veterans and their families. Public-private partnerships have been discussed as a potential solution and could include collaborations between a public agency, such as the VA, and a private organization, such as a veteran service organization, private industry, or private hospital. Despite the call for such partnerships, not much is known about what a public-private partnership would entail for addressing behavioral health concerns for veterans and their families. The health care literature is sparse in this area, and published examples and recommendations are limited. Thus, the authors wrote this article to inform the creation of public-private partnerships to better serve veterans and their families. The article outlines nine key components for public-private partnerships addressing veteran behavioral health care. These components are supported by qualitative interview data from five successful public-private partnerships that serve veterans and their families. This study will assist policymakers in the VA and other federal agencies in developing and fostering public-private partnerships to address the behavioral health care needs of veterans and their families. The article also discusses next steps for research and policymaking efforts with regard to these partnerships.
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- 2017
11. Assessing the Implications of Allowing Transgender Personnel to Serve Openly
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Agnes Gereben Schaefer, Radha Iyengar, Srikanth Kadiyala, Jennifer Kavanagh, Charles C. Engel, Kayla M. Williams, Amii M. Kress, Agnes Gereben Schaefer, Radha Iyengar, Srikanth Kadiyala, Jennifer Kavanagh, Charles C. Engel, Kayla M. Williams, and Amii M. Kress
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- Gay military personnel--Government policy--United States, Transgender military personnel--United States, Intersex military personnel--United States
- Abstract
The U.S. Department of Defense is considering a change in policy to allow transgender military personnel to serve openly. A RAND study examined the health care needs of transgender personnel, the costs of gender transition–related care, and the potential readiness implications of a policy change. The experiences of foreign militaries that permit transgender service members to serve openly also point to some best practices for U.S. policymakers.
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- 2016
12. Integrating Grant-funded Research into the Undergraduate Biology Curriculum Using IMG-ACT
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Megan M. Keller, Xianxian Liu, Kayla M. Williams, Jayna L. Ditty, Rebecca E. Parales, and Grischa Y. Chen
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Annotation ,Documentation ,Undergraduate research ,Active learning ,ComputingMilieux_COMPUTERSANDEDUCATION ,Educational technology ,Wet laboratory ,Engineering ethics ,Context (language use) ,Bioinformatics ,Molecular Biology ,Biochemistry ,Curriculum - Abstract
It has become clear in current scientific pedagogy that the emersion of students in the scientific process in terms of designing, implementing, and analyzing experiments is imperative for their education; as such, it has been our goal to model this active learning process in the classroom and laboratory in the context of a genuine scientific question. Toward this objective, the National Science Foundation funded a collaborative research grant between a primarily undergraduate institution and a research-intensive institution to study the chemotactic responses of the bacterium Pseudomonas putida F1. As part of the project, a new Bioinformatics course was developed in which undergraduates annotate relevant regions of the P. putida F1 genome using Integrated Microbial Genomes Annotation Collaboration Toolkit, a bioinformatics interface specifically developed for undergraduate programs by the Department of Energy Joint Genome Institute. Based on annotations of putative chemotaxis genes in P. putida F1 and comparative genomics studies, undergraduate students from both institutions developed functional genomics research projects that evolved from the annotations. The purpose of this study is to describe the nature of the NSF grant, the development of the Bioinformatics lecture and wet laboratory course, and how undergraduate student involvement in the project that was initiated in the classroom has served as a springboard for independent undergraduate research projects.
- Published
- 2013
13. The Implications of Allowing Transgender Personnel to Serve Openly in the U.S. Military
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Agnes Gereben Schaefer, Radha Iyengar, Amii M. Kress, Jennifer Kavanagh, Srikanth Kadiyala, Kayla M. Williams, and Charles C. Engel
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Gender discrimination ,Military personnel ,U s military ,business.industry ,Potential change ,Political science ,Health care ,Transgender ,Gender studies ,Workforce management ,Public relations ,business ,Workforce diversity - Abstract
To inform a potential change in policy, a RAND study examined the health care needs of transgender military personnel, costs of gender transition–related care and potential readiness implications of allowing transgender personnel to serve openly.
- Published
- 2016
14. Upper airway gene expression shows a more robust adaptive immune response to SARS-CoV-2 in children
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Eran Mick, Alexandra Tsitsiklis, Natasha Spottiswoode, Saharai Caldera, Paula Hayakawa Serpa, Angela M. Detweiler, Norma Neff, Angela Oliveira Pisco, Lucy M. Li, Hanna Retallack, Kalani Ratnasiri, Kayla M. Williamson, Victoria Soesanto, Eric A. F. Simões, Christiana Smith, Lisa Abuogi, Amy Kistler, Brandie D. Wagner, Joseph L. DeRisi, Lilliam Ambroggio, Peter M. Mourani, and Charles R. Langelier
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Science - Abstract
The difference between children and adults in response to SARS-CoV-2 infection is not clearly established. Here the authors use gene expression analysis of nasopharyngeal samples from children and adults and show a higher level of immune response in children compared to adults, including of B and T cell activation.
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- 2022
- Full Text
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15. Integrated host/microbe metagenomics enables accurate lower respiratory tract infection diagnosis in critically ill children
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Eran Mick, Alexandra Tsitsiklis, Jack Kamm, Katrina L. Kalantar, Saharai Caldera, Amy Lyden, Michelle Tan, Angela M. Detweiler, Norma Neff, Christina M. Osborne, Kayla M. Williamson, Victoria Soesanto, Matthew Leroue, Aline B. Maddux, Eric A.F. Simões, Todd C. Carpenter, Brandie D. Wagner, Joseph L. DeRisi, Lilliam Ambroggio, Peter M. Mourani, and Charles R. Langelier
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Infectious disease ,Pulmonology ,Medicine - Abstract
BACKGROUND Lower respiratory tract infection (LRTI) is a leading cause of death in children worldwide. LRTI diagnosis is challenging because noninfectious respiratory illnesses appear clinically similar and because existing microbiologic tests are often falsely negative or detect incidentally carried microbes, resulting in antimicrobial overuse and adverse outcomes. Lower airway metagenomics has the potential to detect host and microbial signatures of LRTI. Whether it can be applied at scale and in a pediatric population to enable improved diagnosis and treatment remains unclear.METHODS We used tracheal aspirate RNA-Seq to profile host gene expression and respiratory microbiota in 261 children with acute respiratory failure. We developed a gene expression classifier for LRTI by training on patients with an established diagnosis of LRTI (n = 117) or of noninfectious respiratory failure (n = 50). We then developed a classifier that integrates the host LRTI probability, abundance of respiratory viruses, and dominance in the lung microbiome of bacteria/fungi considered pathogenic by a rules-based algorithm.RESULTS The host classifier achieved a median AUC of 0.967 by cross-validation, driven by activation markers of T cells, alveolar macrophages, and the interferon response. The integrated classifier achieved a median AUC of 0.986 and increased the confidence of patient classifications. When applied to patients with an uncertain diagnosis (n = 94), the integrated classifier indicated LRTI in 52% of cases and nominated likely causal pathogens in 98% of those.CONCLUSION Lower airway metagenomics enables accurate LRTI diagnosis and pathogen identification in a heterogeneous cohort of critically ill children through integration of host, pathogen, and microbiome features.FUNDING Support for this study was provided by the Eunice Kennedy Shriver National Institute of Child Health and Human Development and the National Heart, Lung, and Blood Institute (UG1HD083171, 1R01HL124103, UG1HD049983, UG01HD049934, UG1HD083170, UG1HD050096, UG1HD63108, UG1HD083116, UG1HD083166, UG1HD049981, K23HL138461, and 5R01HL155418) as well as by the Chan Zuckerberg Biohub.
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- 2023
- Full Text
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16. Integrating grant-funded research into the undergraduate biology curriculum using IMG-ACT
- Author
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Jayna L, Ditty, Kayla M, Williams, Megan M, Keller, Grischa Y, Chen, Xianxian, Liu, and Rebecca E, Parales
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Financing, Organized ,Academies and Institutes ,Computational Biology ,Educational Status ,Humans ,Curriculum ,Genomics ,Students ,Biology ,Education, Medical, Undergraduate - Abstract
It has become clear in current scientific pedagogy that the emersion of students in the scientific process in terms of designing, implementing, and analyzing experiments is imperative for their education; as such, it has been our goal to model this active learning process in the classroom and laboratory in the context of a genuine scientific question. Toward this objective, the National Science Foundation funded a collaborative research grant between a primarily undergraduate institution and a research-intensive institution to study the chemotactic responses of the bacterium Pseudomonas putida F1. As part of the project, a new Bioinformatics course was developed in which undergraduates annotate relevant regions of the P. putida F1 genome using Integrated Microbial Genomes Annotation Collaboration Toolkit, a bioinformatics interface specifically developed for undergraduate programs by the Department of Energy Joint Genome Institute. Based on annotations of putative chemotaxis genes in P. putida F1 and comparative genomics studies, undergraduate students from both institutions developed functional genomics research projects that evolved from the annotations. The purpose of this study is to describe the nature of the NSF grant, the development of the Bioinformatics lecture and wet laboratory course, and how undergraduate student involvement in the project that was initiated in the classroom has served as a springboard for independent undergraduate research projects.
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- 2012
17. Linking Language, Regional Expertise, and Culture Capabilities to Military Readiness
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Sarah O. Meadows, Kayla M. Williams, Mark Overton, Barry Costa, John Bornmann, and Jennifer DeCamp
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Operational readiness ,Knowledge management ,business.industry ,Political science ,Tracking (education) ,business ,Training (civil) - Abstract
Current tracking of language, regional expertise, and culture training and capabilities provides limited support for military decisionmaking.
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- 2012
18. Modified PCR protocol to increase sensitivity for determination of bacterial community composition
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Kayla M. Williamson, Brandie D. Wagner, Charles E. Robertson, Mark J. Stevens, Marci K. Sontag, Peter M. Mourani, and J. Kirk Harris
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16S rRNA gene ,Microbiome ,Amplicon ,Mechanical ventilation ,Microbial ecology ,QR100-130 - Abstract
Abstract Background The objective of this project was to increase the sensitivity of sequence-based bacterial community determination without impacting community composition or interfering with cluster formation during sequencing. Two PCR protocols (standard and modified) were examined in airway samples where we observed a large range in bacterial load (3.1–6.2 log10 16S rRNA gene copies/reaction). Tracheal aspirate (TA) samples (n = 99) were collected from sixteen children requiring mechanical ventilation at a single center. DNA was extracted, and total bacterial load (TBL) was assessed using qPCR. Amplification of 16S rRNA was attempted with both protocols in all samples. Results PCR product was observed using both protocols in 52 samples and in 24 additional samples only with the modified protocol. TBL, diversity metrics, and prominent taxa were compared for samples in three groups based on success of the two protocols (successful with both, success with modified only, unsuccessful for both). TBL differed significantly across the three groups (p
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- 2021
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19. Invisible Wounds of War: Psychological and Cognitive Injuries, Their Consequences, and Services to Assist Recovery
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Beau Kilmer, Beau Kilmer, Benjamin R. Karney, Christine Eibner, Claudia Diaz, David M. Adamson, Elizabeth D'Amico, Gail Fisher, Grant N. Marshall, Jeanne S. Ringel, Jerry M. Sollinger, Karen Chan Osilla, Karen N. Metscher, Kayla M. Williams, Laure T. Martin, Leah B. Caldarone, Lisa H. Jaycox, Lisa S. Meredith, Mary E. Vaiana, Michael R. Yochelson, M. Audrey Burnam, Rachel M. Burns, Rajeev Ramchand, Robert A. Cox, Rosalie Liccardo Pacula, Terri Tanielian, Terry L. Schell, Todd C. Helmus, Beau Kilmer, Beau Kilmer, Benjamin R. Karney, Christine Eibner, Claudia Diaz, David M. Adamson, Elizabeth D'Amico, Gail Fisher, Grant N. Marshall, Jeanne S. Ringel, Jerry M. Sollinger, Karen Chan Osilla, Karen N. Metscher, Kayla M. Williams, Laure T. Martin, Leah B. Caldarone, Lisa H. Jaycox, Lisa S. Meredith, Mary E. Vaiana, Michael R. Yochelson, M. Audrey Burnam, Rachel M. Burns, Rajeev Ramchand, Robert A. Cox, Rosalie Liccardo Pacula, Terri Tanielian, Terry L. Schell, and Todd C. Helmus
- Abstract
Since October 2001, approximately 1.64 million U.S. troops have been deployed for Operations Enduring Freedom and Iraqi Freedom (OEF/OIF) in Afghanistan and Iraq. Early evidence suggests that the psychological toll of these deployments may be disproportionately high compared with the physical injuries of combat. Research has focused primarily on three conditions: post-traumatic stress disorder (PTSD), depression, and traumatic brain injury (TBI). Karney et al. review the empirical literature on these three conditions, focusing on research that supports projections about the likely outcomes for OEF/OIF veterans and their families. These include an increased risk of suicide, substance abuse, and cardiovascular disease. Mental health conditions among veterans are also associated with reduced work productivity and future job prospects and may be a precursor to homelessness. Post-combat mental health disorders also affect servicemembers' spouses and children: For example, each of the three disorders has been linked to intimate partner violence and divorce. The authors also emphasize that it is common for veterans with one of the three conditions -- PTSD, depression, or TBI -- to also develop another of the three, and such individuals tend to experience more severe symptoms, poorer treatment outcomes, and more disability in social and occupation function. Karney et al. conclude with two series of recommendations: one for future research, and one for policy and interventions to mitigate the consequences of post-combat mental health conditions.
- Published
- 2008
20. Impact of a Dedicated Teaching Attending Experience on a Required Emergency Medicine Clerkship
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Todd A. Guth, Michael C. Overbeck, Kelley Roswell, Tien T. Vu, Kayla M. Williamson, Yeonjoo Yi, William Hilty, and Jeff Druck
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Medicine ,Medical emergencies. Critical care. Intensive care. First aid ,RC86-88.9 - Abstract
Introduction: One published strategy for improving educational experiences for medical students in the emergency department (ED) while maintaining patient care has been the implementation of dedicated teaching attending shifts. To leverage the advantages of the ED as an exceptional clinical educational environment and to address the challenges posed by the rapid pace and high volume of the ED, our institution developed a clerkship curriculum that incorporates a dedicated clinical educator role – the teaching attending – to deliver quality bedside teaching experiences for students in a required third-year clerkship. The purpose of this educational innovation was to determine whether a dedicated teaching attending experience on a third-year required emergency medicine (EM) clerkship would improve student-reported clinical teaching evaluations and student-reported satisfaction with the overall quality of the EM clerkship. Methods: Using a five-point Likert-type scale (1 - poor to 5 - excellent), student-reported evaluation ratings and the numbers of graduating students matching into EM were trended for 10 years retrospectively from the inception of the clerkship for the graduating class of 2009 through and including the graduating class of 2019. We used multinomial logistic regression to evaluate whether the presence of a teaching attending during the EM clerkship improved student-reported evaluation ratings for the EM clerkship. We used sample proportion tests to assess the differences between top-box (4 or 5 rating) proportions between years when the teaching attending experience was present and when it was not. Results: For clinical teaching quality, when the teaching attending is present the estimated odds of receiving a rating of 5 is 77.2 times greater (p
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- 2019
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21. HIV-Exposed Uninfected Infants Have Increased Regulatory T Cells That Correlate With Decreased T Cell Function
- Author
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Emilie Jalbert, Kayla M. Williamson, Miranda E. Kroehl, Michael J. Johnson, Clare Cutland, Shabir A. Madhi, Marta C. Nunes, and Adriana Weinberg
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HIV-exposed uninfected infants ,regulatory T cells ,T cell function ,antigen presenting cells ,T cell differentiation ,Immunologic diseases. Allergy ,RC581-607 - Abstract
Background: HIV-exposed uninfected infants (HEU) are at higher risk of severe infections, hospitalizations and death compared with HIV-unexposed uninfected infants (HUU), but the immune deficit underlying it is not known. To address this gap, we investigated T cell functionality and its relationship to phenotypic profiles of T cells and antigen presenting cells (APC) in HEU and HUU.Methods: Blood mononuclear cells from 55 HEU and 16 HUU were stimulated with Staphylococcal Enterotoxin B (SEB) or mock for 72 h, and tested by flow cytometry for proliferation and expression of Th1, Th2, and regulatory (Treg) markers. In parallel, cells were phenotypically assessed for differentiation profiles of Treg, conventional T cell (Tconv) and APC in unstimulated cells.Results: HEU had lower CD4+ functional responses to SEB/mock and similar CD8+ responses compared with HUU. In the phenotypic T cell panel, HEU showed higher proportions of CD4+ and CD8+ Treg expressing IL10, FOXP3, and CD25; higher effector Tconv and Treg; and lower naïve and CD4+TGFβ+ Treg compared with HUU. In the phenotypic APC panel, HEU showed higher proportions of CD1c+ cDC2, CD123+ pDC, CD16+ inflammatory monocytes and cDC and higher expression of CD103 on CD1c-CD123-CD16-cDC1 compared with HUU. Regression analyses adjusted for HIV exposure and multiple comparisons showed that higher CD8+IL10+ and CD8+FOXP3+ Treg in unstimulated cells were associated with lower CD8+ T cell functional responses to SEB/mock. Functionality was not affected by Tconv differentiation, but higher APC activation in aggregate was associated with higher CD8+IL10+ Treg responses to SEB.Conclusions: T cell functionality was decreased in HEU compared with HUU. High CD8+ Treg proportions were the most important predictors of decreased T cell functionality in HEU and HUU.
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- 2019
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22. Impact of enzymatic digestion on bacterial community composition in CF airway samples
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Kayla M. Williamson, Brandie D. Wagner, Charles E. Robertson, Emily J. Johnson, Edith T. Zemanick, and J. Kirk Harris
- Subjects
16s Sequencing ,Microbiome ,Sputum ,Gram positive ,Beta diversity ,DNA extraction ,Medicine ,Biology (General) ,QH301-705.5 - Abstract
Background Previous studies have demonstrated the importance of DNA extraction methods for molecular detection of Staphylococcus, an important bacterial group in cystic fibrosis (CF). We sought to evaluate the effect of enzymatic digestion (EnzD) prior to DNA extraction on bacterial communities identified in sputum and oropharyngeal swab (OP) samples from patients with CF. Methods DNA from 81 samples (39 sputum and 42 OP) collected from 63 patients with CF was extracted in duplicate with and without EnzD. Bacterial communities were determined by rRNA gene sequencing, and measures of alpha and beta diversity were calculated. Principal Coordinate Analysis (PCoA) was used to assess differences at the community level and Wilcoxon Signed Rank tests were used to compare relative abundance (RA) of individual genera for paired samples with and without EnzD. Results Shannon Diversity Index (alpha-diversity) decreased in sputum and OP samples with the use of EnzD. Larger shifts in community composition were observed for OP samples (beta-diversity, measured by Morisita-Horn), whereas less change in communities was observed for sputum samples. The use of EnzD with OP swabs resulted in significant increase in RA for the genera Gemella (p
- Published
- 2017
- Full Text
- View/download PDF
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