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The paper shows a new idea of aluminium alloys. New alloys with specially selected alloying element i.e. silver have electrical conductivity similar to pure aluminium at ambient temperature and better than pure aluminium electrical conductivity at increased temperatures. Al-Ag alloys for electrical applications (mainly for electrical conductors) due to high electrical conductivity at increased temperatures at the level of the maximum conductor working temperatures give possibility of better current capacity of conductors.

Currently we can observe a worldwide trend to find new materials with extraordinary properties. In particular these researches are aimed to find a method to improve electrical conductivity, mechanical properties, corrosion resistance and rheological behavior of known materials. This effect can be achieved by a synthesis of modern carbon materials with metals. In this paper authors presented research results of synthesis process for Cu-graphene and Cu-CNT’s composites obtained by the mechanical synthesis in cold drawing process. The article presents also the results of electrical conductivity measurements and structural analysis of carbon particles presence in copper matrix. The research has shown that obtained composites have electrical conductivity lower than used copper base material. Additionally, the structural analysis has shown that after the drawing process carbon materials particles are mechanically pressed into Cu in the matrix, and these particles do not participate in the current flow, creating an actual barrier for electrons transport.

Structural connections are one of the most important parts influencing the overall performance of a wooden structure. The way of design of these can lead both to increasing and decreasing internal stresses occurring in the load-carrying elements and structure’s total deformations. Typical mechanical joints in wooden structures are defined as plastic hinges or, at best, semi-rigid. The innovative hybrid one proposed in the paper with adhesive added between elements can be much stiffer than typical connection, which can lead to assuming rigid joint and significant reduction in stresses and deformations of a structure. The research comprised 30 specimens in three groups (10 per each group: reference - without adhesive, hybrid with one-component PUR - polyurethane adhesive and hybrid with one-component PVAc – polyvinyl acetate adhesive) tested on the MTS 809 testing machine up to failure. An innovative idea was to connect elements initially by applying an accurately predicted tightening torque value to bolts. This resulted in obtaining enough clamping pressure between elements for adhesive curing, with none other equipment. The load was applied in parallel-to-grain wood direction. The results showed that utilising hybrid connection caused, both for PUR and PVAc adhesive, a huge increase in stiffness. When comparing to the reference no-adhesive, bolted connection, this was 2365% stiffer (nearly 24 times). Load-carrying capacity was higher too, however, the increase was not that significant and was at the level of 14.4% and 27.1%, for PUR and PVAc adhesives, consecutively. Worth noting is that the hybrid connection could continue to work after adhesive failure with 60% higher stiffness than the reference one and its load-carrying capacity was only 10% lower than the reference. Hybrid connections of this type can potentially serve as structural joints because of the innovative concept of combining components. Steel plates can be covered with adhesive and then inserted between wooden parts. Next, the tightened bolts can work as clamps producing enough pressure for adhesive curing, enabling the joint to be assembled directly on the construction site. Despite the mentioned advantages, before providing the connections’ design methods, the idea needs to be tested towards various effects influencing wooden structures. Incorporating numerical modelling can be extremely important too.

Due to their very wide range of indications, anticoagulants are one of the most commonly used drug groups. Although these drugs are characterized by different mechanisms of action, the most common complication of their use is still bleeding episodes, the frequency of which depends largely on the clinical condition of the patient using such therapy. For this reason, to this day, the best method of preventing bleeding complications remains the assessment of bleeding risk using scales such as HAS-BLED. There are many reports in the literature assessing the occurrence of this type of complication after the use of drugs affecting the coagulation process, as well as many reports comparing individual groups of drugs with different mechanisms of action. However, there are still no clear guidelines that would indicate which group of anticoagulants should be preferred in particular groups of patients. The aim of our article is to summarize the data collected so far regarding the safety of using specific groups of anticoagulants and the frequency of bleeding complications after their use.

CuNiSi alloys are widely used in various mechanical and electrical applications. These group of materials, due to the phenomena of precipitation hardening, are able to obtain high mechanical properties with also relatively high electrical properties. In the article authors compare two different types of products, made from the CW111C alloy i.e. rods which were continuously cast on the horizontal laboratory casting set-up (low degree of structure refinement) and rods commercially extruded with high degree of structure refinement. The presented results of experimental work characterize the tested materials in terms of their chemical composition, mechanical and electrical properties depending on the manufacturing process, as well as reveal their structures and the effect of heat treatment on the morphology of the Ni-Si precipitates.

Cultured meat has potential to diversify methods for protein production, but innovations in production efficiency will be required to make cultured meat a feasible protein alternative. Microcarriers provide a strategy to culture sufficient volumes of adherent cells in a bioreactor that are required for meat products. However, cell culture on inedible microcarriers involves extra downstream processing to dissociate cells prior to consumption. Here, we present edible microcarriers that can support the expansion and differentiation of myogenic cells in a single bioreactor system. To fabricate edible microcarriers with a scalable process, we used water-in-oil emulsions as templates for gelatin microparticles. We also developed a novel embossing technique to imprint edible microcarriers with grooved topology in order to test if microcarriers with striated surface texture can promote myoblast proliferation and differentiation in suspension culture. In this proof-of-concept demonstration, we showed that edible microcarriers with both smooth and grooved surface topologies supported the proliferation and differentiation of mouse myogenic C2C12 cells in a suspension culture. The grooved edible microcarriers showed a modest increase in the proliferation and alignment of myogenic cells compared to cells cultured on smooth, spherical microcarriers. During the expansion phase, we also observed the formation of cell-microcarrier aggregates or 'microtissues' for cells cultured on both smooth and grooved microcarriers. Myogenic microtissues cultured with smooth and grooved microcarriers showed similar characteristics in terms of myotube length, myotube volume fraction, and expression of myogenic markers. To establish feasibility of edible microcarriers for cultured meat, we showed that edible microcarriers supported the production of myogenic microtissue from C2C12 or bovine satellite muscle cells, which we harvested by centrifugation into a cookable meat patty that maintained its shape and exhibited browning during cooking. These findings demonstrate the potential of edible microcarriers for the scalable production of cultured meat in a single bioreactor.

Abstract While mortality is often the primary focus of pathogen virulence, non‐lethal consequences, particularly for male reproductive fitness, are less understood; however, they are essential for understanding how sexual selection contributes to promoting resistance. We investigated how the fungal pathogen Metarhizium brunneum affects mating ability, fertility, and seminal fluid protein (SFP) expression of male Drosophila melanogaster paired with highly receptive virgin females in non‐competitive settings. Depending on sex and dose, there was a 3–6‐day incubation period after infection, followed by an abrupt onset of mortality. Meanwhile, the immune response was strongly induced already 38 h after infection and continued to increase as infection progressed. Latency to mate somewhat increased during the incubation period compared to sham‐treated males, but even on Day 5 post infection >90% of infected males mated within 2 h. During the incubation period, M. brunneum infection reduced male reproductive potential (the number of offspring sired without mate limitation) by 11%, with no clear increase over time. Approaching the end of the incubation period, infected males had lower ability to convert number of mating opportunities into number of offspring. After repeated mating, infected males had lower SFP expression than sham controls, more so in males that mated with few mates 24 h earlier. Overall, despite strong activation of the immune response, males' mating ability and fertility remained surprisingly little affected by the fungal infection, even shortly before the onset of mortality. This suggests that the selection for resistance acts mainly through mortality, and the scope for fertility selection to enhance resistance in non‐competing settings is rather limited.

We investigate the contribution of a candidate gene, fiz (fezzik), to complex polygenic adaptation to juvenile malnutrition in Drosophila melanogaster. Experimental populations maintained for >250 generations of experimental evolution to a nutritionally poor larval diet (Selected populations) evolved several-fold lower fiz expression compared to unselected Control populations. Here we show that this divergence in fiz expression is mediated by a cis-regulatory polymorphism. This polymorphism, originally sampled from a natural population in Switzerland, is distinct from a second cis-regulatory SNP previously identified in non-African D. melanogaster populations, implying that two independent cis-regulatory variants promoting high fiz expression segregate in non-African populations. Enzymatic analyses of Fiz protein expressed in E. coli demonstrate that it has ecdysone oxidase activity acting on both ecdysone and 20-hydroxyecdysone. Four of five fiz paralogs annotated to ecdysteroid metabolism also show reduced expression in Selected larvae, implying that malnutrition-driven selection favored general downregulation of ecdysone oxidases. Finally, as an independent test of the role of fiz in poor diet adaptation, we show that fiz knockdown by RNAi results in faster larval growth on the poor diet, but at the cost of greatly reduced survival. These results imply that downregulation of fiz in Selected populations was favored by selection on the nutritionally poor diet because of its role in suppressing growth in response to nutrient shortage. However, they suggest that fiz downregulation is only adaptive in combination with other changes evolved by Selected populations, which ensure that the organism can sustain the faster growth promoted by fiz downregulation.

Abstract Glucose is a universally available inexpensive biomarker, which is increased as part of the physiological stress response to acute myocardial infarction (AMI) and may therefore help in its early diagnosis. To test this hypothesis, glucose, high-sensitivity cardiac troponin (hs-cTn) T, and hs-cTnI were measured in consecutive patients presenting with acute chest discomfort to the emergency department (ED) and enrolled in a large international diagnostic study (NCT00470587). Two independent cardiologists centrally adjudicated the final diagnosis using all clinical data, including serial hs-cTnT measurements, cardiac imaging and clinical follow-up. The primary diagnostic endpoint was index non-ST-segment elevation MI (NSTEMI). Prognostic endpoints were all-cause death, and cardiovascular (CV) death or future AMI, all within 730-days. Among 5639 eligible patients, NSTEMI was the adjudicated final diagnosis in 1051 (18.6%) patients. Diagnostic accuracy quantified using the area under the receiver-operating characteristics curve (AUC) for the combination of glucose with hs-cTnT and glucose with hs-cTnI was very high, but not higher versus that of hs-cTn alone (glucose/hs-cTnT 0.930 [95% CI 0.922–0.937] versus hs-cTnT 0.929 [95% CI 0.922–0.937]; glucose/hs-cTnI 0.944 [95% CI 0.937–0.951] versus hs-cTnI 0.944 [95% CI 0.937–0.951]). In early-presenters, a dual-marker strategy (glucose

Background: Medical personnel carrying out electrical cardioversion (EC) procedures must remember to have the R-wave sync mode switched on, use the correct energy and maintain personal safety. The defibrillators used by medical response teams most often switch out of cardioversion mode once a shock is delivered. Therefore, this mode must be switched on again before subsequent shocks are delivered. The main aim of the study was to assess the ability of emergency medical teams participating in emergency medicine championships to perform EC. Methods: The research was a retrospective observational study and was based on an analysis of the evaluation sheets from two tasks simulating the management of a patient with unstable tachycardia conducted during the International Winter Emergency Medicine Championships. Three-person teams consisting of paramedics and representing the Polish emergency services were included in the study. The team representing the championship organiser and the few foreign teams participating in the competition were excluded from the study. Results: The decision to conduct EC was taken by 36 teams (83.72%) in 2015 and 27 teams (87.10%) in 2019. In both editions of the championships, during consecutive shocks, the percentage of actions performed correctly decreased significantly—switching on synchronisation mode in 2015 (94.4%, 83.33%, 72.22%) and in 2019 (100%, 88.89%, 81.48%); correct energies in 2015 (91.67%, 80.56%, 77.78%) and in 2019 (92.59%, 85.19%, 81.48%); shocks in a safe manner in 2015 (94.44%, 94.44%, 91.67%) and in 2019 (100%, 96.30%, 96.30%). Conclusions: Teams participating in the assessed tasks in a significant majority of cases correctly qualified the patient for EC, and correctly carried out the actions required for this procedure. It is of particular note that with every subsequent shock, the percentage of shocks carried out without the sync mode increased significantly.

From the moment the SARS-CoV-2 virus was identified in December 2019, the COVID-19 disease spread around the world, causing an increase in hospitalisations and deaths. From the beginning of the pandemic, scientists tried to determine the major cause that led to patient deaths. In this paper, the background to creating a research model was diagnostic problems related to early assessment of the degree of damage to the lungs in patients with COVID-19. The study group comprised patients hospitalised in one of the temporary COVID hospitals. Patients admitted to the hospital had confirmed infection with SARS-CoV-2. At the moment of admittance, arterial blood was taken and the relevant parameters noted. The results of physical examinations, the use of oxygen therapy and later test results were compared with the condition of the patients in later computed tomography images and descriptions. The point of reference for determining the severity of the patient’s condition in the computer imagery was set for a mild condition as consisting of a percentage of total lung parenchyma surface area affected no greater than 30%, an average condition of between 30% and 70%, and a severe condition as greater than 70% of the lung parenchyma surface area affected. Patients in a mild clinical condition most frequently had mild lung damage on the CT image, similarly to patients in an average clinical condition. Patients in a serious clinical condition most often had average levels of damage on the CT image. On the basis of the collected data, it can be said that at the moment of admittance, BNP, PE and HCO3− levels, selected due to the form of lung damage, on computed tomography differed from one another in a statistically significant manner (p < 0.05). Patients can qualify for an appropriate group according to the severity of COVID-19 on the basis of a physical examination and applied oxygen therapy. Patients can qualify for an appropriate group according to the severity of COVID-19 on the basis of BNP, HCO3 and BE parameters obtained from arterial blood.

Scientists do a variety of laboratory tests on timber and wood-containing composites. An example is adhesively bonded joints in such materials. Despite a wide range of empirical research, consideration of adhesive layers or surfaces in a structure is commonly done in a very simplified manner - they are often modelled as a perfect connection between adherends. It means the cohesive stiffness and opportunity of progressive delamination are neglected. This may lead to an overestimation of the structural load-bearing capacity. The article presents wood-wood and wood-CFRP adhesively bonded joints’ investigations, based on own experimental testing technique (covering a current one as a Digital Image Correlation), analytical double-lap model for adhesives and advanced numerical Finite Element approach. The aim of the paper is to give the guidelines for complex, non-linear modelling of connections in glue laminated timber and wood-CFRP composites that can be utilised for many purposes.

Due to their very wide range of indications, anticoagulants are one of the most commonly used drug groups. Although these drugs are characterized by different mechanisms of action, the most common complication of their use is still bleeding episodes, the frequency of which depends largely on the clinical condition of the patient using such therapy. For this reason, to this day, the best method of preventing bleeding complications remains the assessment of bleeding risk using scales such as HAS-BLED. There are many reports in the literature assessing the occurrence of this type of complication after the use of drugs affecting the coagulation process, as well as many reports comparing individual groups of drugs with different mechanisms of action. However, there are still no clear guidelines that would indicate which group of anticoagulants should be preferred in particular groups of patients. The aim of our article is to summarize the data collected so far regarding the safety of using specific groups of anticoagulants and the frequency of bleeding complications after their use. [ABSTRACT FROM AUTHOR]

Juvenile undernutrition has lasting effects on adult metabolism of the affected individuals, but it is unclear how adult physiology is shaped over evolutionary time by natural selection driven by juvenile undernutrition. We combined RNAseq, targeted metabolomics, and genomics to study the consequences of evolution under juvenile undernutrition for metabolism of reproductively active adult females of Drosophila melanogaster. Compared to Control populations maintained on standard diet, Selected populations maintained for over 230 generations on a nutrient-poor larval diet evolved major changes in adult gene expression and metabolite abundance, in particular affecting amino acid and purine metabolism. The evolved differences in adult gene expression and metabolite abundance between Selected and Control populations were positively correlated with the corresponding differences previously reported for Selected versus Control larvae. This implies that genetic variants affect both stages similarly. Even when well fed, the metabolic profile of Selected flies resembled that of flies subject to starvation. Finally, Selected flies had lower reproductive output than Controls even when both were raised under the conditions under which the Selected populations evolved. These results imply that evolutionary adaptation to juvenile undernutrition has large pleiotropic consequences for adult metabolism, and that they are costly rather than adaptive for adult fitness. Thus, juvenile and adult metabolism do not appear to evolve independently from each other even in a holometabolous species where the two life stages are separated by a complete metamorphosis.

The results of the current paper present the research concerning the obtaining of Cu-Ag alloys with wt. % of silver ranging between 5 and 20 % using continuous casting process. The process parameters have been introduced. Selected results regarding physical and mechanical properties of the casts have been discussed. Strength coefficients describing the plastic deformation range of the Cu-Ag alloys have been calculated using Hollomon’s equation. Additionally, in order to determine the influence of the temperature with Differential Scanning Calorimeter (DSC) on the electrical resistance, the values of temperature coefficients of resistance have been determined for all of the tested alloys.

Abstract An important element in the effective treatment of patients infected with the SARS-CoV-2 virus during the pandemic is an effective early triage to determine patient allocation and in-patient therapy. This paper assesses the prognostic value of capillary blood gas tests in predicting extended hospitalisation and death due to COVID-19. This retrospective statistical research is based on a group of 200 patients, hospitalised from 15 October 2020 to 08 March 2021. The study utilised the treatment documentation of these patients hospitalised due to COVID-19 at the Pulmonology and Thoracic Surgery Centre in Bystra (Southern Poland) during this period. The hospital has 50 beds with access to oxygen for COVID-19 patients and a five-bed intensive care unit. On the basis of the obtained results, conclusions were drawn that the need for early oxygen therapy with an oxygen mask and low pH values in capillary blood are significant risk factors for prolonging hospitalisation due to COVID-19. Age, the need for early oxygen mask therapy and low oxygen saturation are important risk factors for death from COVID-19. Capillary blood gas analysis is a simple and effective method of early in-patient segregation of COVID-19 patients.

With the increasing popularity of yachting sports and races comes the need to develop a more advanced and efficient propulsion device. Significant improvement can be made when using a mainly lift-driven propulsion source, known as a wing sail. This idea, dating back as far as the mid-70s, is nowadays regaining interest as a propulsion system in multihull, high-performance racing vessels (for instance, the AC50 and AC72 America’s Cup yacht classes). This article documents 2D and 3D numerical analyses of wing sail systems imitating those of an AC72 racing yacht class. It depicts methods employed in two- and three-dimensional steady-state flow simulations, compares systems equipped with various geometries of mainsails, and details a comprehensive examination of the airflow around the vessel using spatial analyses. Numerical calculations were carried out using ANSYS CFX and ANSYS Fluent (with overset feature) for 2D and 3D models, respectively. All simulations were conducted under conditions similar to those acting on the real system, i.e., high Reynolds number (order of magnitude 106 to 107) and atmospheric boundary layer (in the 3D model).

Citric acid and erythritol are obtained on an industrial scale using biotechnological methods. Due to the growing market demand for these products, research is underway to improve the process economics by introducing new microorganisms, in particular of the species Yarrowia lipolytica. The aim of this study was to evaluate transformants of Y. lipolytica for growth and ability to overproduce citric acids and erythritol from glycerol. The transformants were constructed by overexpressing glycerol kinase, methylcitrate synthase and mitochondrial succinate-fumarate transporter in the mutant Wratislavia 1.31. Next, strains were assessed for biosynthesis of citrate (pH 5.5; nitrogen limitation) and erythritol (pH 3.0; high osmotic pressure) from glycerol. Regardless of culture conditions strains, 1.31.GUT1/6 and 1.31.GUT1/6.CIT1/3 exhibited high rates of substrate utilization. Under conditions favoring citrate biosynthesis, both strains produced several percent more citrates, accompanied by higher erythritol production compared to the parental strain. During erythritol biosynthesis, the strain 1.31.GUT1/6.CIT1/3.E34672g obtained as a result of co-expression of all three genes stood out, producing 84.0 g/L of erythritol with yield and productivity of 0.54 g/g and 0.72 g/Lh, respectively, which places it in the group of the highest-ranked producers of erythritol among Y. lipolytica species.

The article presents the research results of current cyclic thermal tests performed on enamelled aluminium wires connections, made with the use of a new type of Shark-Al quick-connectors. In particular, the main purpose of the conducted research was to analyse the effect of cyclic heating on the contact resistance change during the tests which allows to test the stability of the connections in simulated working conditions under current flow. Tests included a total of 300 thermal cycles, of which the first 200 were carried out to the 65 °C temperature measured in the connector at above the ambient temperature, and then additional 100 cycles were performed for the temperature of the connector at 140 °C (tolerance +5 °C). During the tests, the resistance of samples was monitored, which allowed to verify the correctness of performed connections.

We prove the convergence of adaptive discontinuous Galerkin and [Formula omitted]-interior penalty methods for fully nonlinear second-order elliptic Hamilton-Jacobi-Bellman and Isaacs equations with Cordes coefficients. We consider a broad family of methods on adaptively refined conforming simplicial meshes in two and three space dimensions, with fixed but arbitrary polynomial degrees greater than or equal to two. A key ingredient of our approach is a novel intrinsic characterization of the limit space that enables us to identify the weak limits of bounded sequences of nonconforming finite element functions. We provide a detailed theory for the limit space, and also some original auxiliary functions spaces, that is of independent interest to adaptive nonconforming methods for more general problems, including Poincaré and trace inequalities, a proof of the density of functions with nonvanishing jumps on only finitely many faces of the limit skeleton, approximation results by finite element functions and weak convergence results., Author(s): Ellya L. Kawecki [sup.1], Iain Smears [sup.1] Author Affiliations: (1) grid.83440.3b, 0000000121901201, Department of Mathematics, University College London, , London, UK Introduction > We study the convergence of a [...]