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23 results on '"Kawakami, Ai"'

7. Type II Natural Killer T Cells that Recognize Sterol Carrier Protein 2 Are Implicated in Vascular Inflammation in the Rat Model of Systemic Connective Tissue Diseases

Author

Nishioka, Yusuke, Yamaguchi, Madoka, Kawakami, Ai, Munehiro, Maya, Masuda, Sakiko, 1000020360901, Tomaru, Utano, 1000060321957, Ishizu, Akihiro, Nishioka, Yusuke, Yamaguchi, Madoka, Kawakami, Ai, Munehiro, Maya, Masuda, Sakiko, 1000020360901, Tomaru, Utano, 1000060321957, and Ishizu, Akihiro

Abstract

We previously generated a rat model that developed systemic connective tissue diseases, including synovitis, myositis, and small-vessel vasculitis (SVV), and established a vascular endothelial cell–reactive T-cell clone, VASC-1, from the model. VASC-1 was determined to be a type II natural killer T-cell clone. In this study, we attempted to identify the antigen recognized by VASC-1. The monkey-derived cell line COS-7 was used because VASC-1 does not bind naturally to COS-7, although the amino acid sequences are well conserved between monkey CD1d and rat CD1d. We generated 98 COS-7 clones transfected with miscellaneous rat cDNA and screened them for VASC-1 binding. Consequently, we found one clone, 4D2, which could bind to VASC-1. Sequencing identified the rat cDNA introduced into 4D2 as sterol carrier protein 2 (SCP2). When VASC-1 was co-cultured with SCP2 knockdown rat vascular endothelial cells, VASC-1 binding was reduced significantly. Moreover, we designed a series of rat SCP2 peptides and introduced them into COS-7 cells. On the basis of VASC-1 binding and proliferation, we revealed that the peptide rSCP2518-532 included the epitope recognized by VASC-1. Furthermore, immunization with rSCP2518-532 accelerated the development of SVV in the rat model. The collective findings suggest that type II natural killer T cells reactive with autologous SCP2 are implicated in vascular inflammation in the rat model.

Published
2017

10. Indium-Catalyzed Amide Allylation of N-Carbonyl Imides: Formation of Azaspiro-γ-lactones via Ring Opening–Reclosure

Author

Sengoku, Tetsuya, Murata, Yusuke, Aso, Yuwa, Kawakami, Ai, Inuzuka, Toshiyasu, Sakamoto, Masami, Takahashi, Masaki, and Yoda, Hidemi

Abstract

A novel and facile synthesis of azaspiro-γ-lactones with a methylene–lactam framework from N-carbonyl imides is described. Mechanistic investigations provide evidence for a two-step reaction process involving ZnCl2-promoted addition of β-amido allylindium species followed by an unexpectedly molecular-sieves-mediated ring opening–reclosure concomitantly with the loss of an N-carbonyl unit.

Published
2024
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15. Establishment of a vascular endothelial cell-reactive type II NKT cell clone from a rat model of autoimmune vasculitis

Author

Iinuma, Chihiro, primary, Waki, Masashi, additional, Kawakami, Ai, additional, Yamaguchi, Madoka, additional, Tomaru, Utano, additional, Sasaki, Naomi, additional, Masuda, Sakiko, additional, Matsui, Yuki, additional, Iwasaki, Sari, additional, Baba, Tomohisa, additional, Kasahara, Masanori, additional, Yoshiki, Takashi, additional, Paletta, Daniel, additional, Herrmann, Thomas, additional, and Ishizu, Akihiro, additional

Published
2014
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17. Why We Like Sad Music

Author

Kawakami, Ai

Subjects
Emotions -- Analysis, Music -- Influence -- Psychological aspects, Sadness -- Analysis, General interest, News, opinion and commentary
Abstract

SADNESS is an emotion we usually try to avoid. So why do we choose to listen to sad music? Musicologists and philosophers have wondered about this. Sad music can induce [...]

Published
2013

22. Musical Emotions

Author

KAWAKAMI, Ai, primary, FURUKAWA, Kiyoshi, additional, OHMURA, Hidefumi, additional, and OKANOYA, Kazuo, additional

Published
2011
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