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Objective: The study aimed to evaluate the effect of Lawsonia inermis flower extract in an Alzheimer’s disease (AD)-induced zebrafish model. Materials and Methods: A total of 60 adult fishes were segregated into five groups (n = 12) and treated with various dilutions of L. inermis extract, respectively. The study was conducted in two phases. The effective dilution range was identified in Phase I (7 days), and the respective dilution was used in Phase II (14 days) for sodium valproate-induced AD. The treatment groups were screened for anxiety, motor coordination, and memory using a novel tank diving test, swim velocity, and maze test, respectively. Treated fishes from each group were investigated for histopathology examination. The results were analyzed with a one-way analysis of variance followed by a post hoc Tukey multiple comparison test using GraphPad Prism. Results: In Phase I, a dose-dependent reduction of degeneration was observed in the pathology of the brain with no significant changes in cognition. In Phase II, the effective dose (8 μg/day/0.5 mg fish) of L. inermis flower extract has shown a significant reduction in anxiety, degeneration, and motor dysfunction with a significant increase in memory and learning. Conclusions: The current investigation has shown that the L. inermis flower extract has a neuroprotective impact on AD zebrafish.

Introduction: Vitex negundo has a myriad of medicinal uses, such as antioxidant, analgesic and anthelminthic properties, and it is used for dysmenorrhoea. The plant contains various phytochemicals, such as flavonoids, vitamins and casticin. All components of the plant are utilised medicinally due to the minimal occurrence of adverse drug reactions, making it a potential drug target for various chronic diseases. However, the role of this plant in neurodegenerative disorders, such as Alzheimer’s Disease (AD), has not yet been extensively studied. Aim: To evaluate the in-silico and in-vitro activity of the anti-Alzheimer properties of the Methanolic Extract of Vitex negundo (MEVN) leaves. Materials and Methods: The present in-silico docking study and in-vitro study were conducted in the Department of Pharmacology at Sri Ramachandra Institute of Higher Education and Research, Porur, Chennai, Tamil Nadu, India, over a period of two months. The in-silico analysis was performed to determine the intermolecular interactions between the ligand and protein of the top five scored molecules using PyMol software. To assess the Acetylcholinesterase (AChE) inhibitory property, the findings were presented as docking scores. Cell viability and cytotoxicity were evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, with results expressed as percentages for various concentrations: 6.25 μg/mL, 12.5 μg/mL, 25 μg/mL, 50 μg/mL, 75 μg/mL, and 100 μg/mL of MEVN. Results: The MEVN exhibited competitive inhibition of the AChE enzyme. There were active interactions between Agnuside, Isochlorogenic Acid B, Isochlorogenic Acid C, Kaempferol-3-O-rutinoside, and Quercetin found in the MEVN with AChE. The percentage of cell viability for the concentrations of 6.25 μg/mL, 12.5 μg/mL, 25 μg/mL, 50 μg/mL, 75 μg/mL, and 100 μg/mL, as determined by the MTT assay, was 98.62%, 98.86%, 97.19%, 96.66%, 91.86%, and 78.82%, respectively. The results indicated that MEVN does not exhibit any toxicity. Conclusion: The findings demonstrated that MEVN possesses AChE inhibitory properties and maintains cell viability, both of which are indicative of anti-Alzheimer activity. Therefore, MEVN can be further evaluated for its potential in preventing cell cytotoxicity and treating AD.

Unmanned aerial vehicles (UAVs) represent a cutting-edge technology that holds the promise of revolutionizing the conventional tasks carried out in the realm of agriculture. On a global scale, UAVs are gaining prominence for pesticide applications, particularly with a focus on utilizing low spray volumes. Nevertheless, there remains a notable gap in research concerning the impacts of employing low spray volumes on herbicide efficacy. Hence, multi-location field studies were conducted at Agricultural Research Station, Bhavanisagar and Wetland Station, Coimbatore, Tamil Nadu, India in 2022 to evaluate the impact of different spray volumes using UAV and Knapsack Manual Sprayer (KMS) on droplet deposition, droplet density, and weed control efficacy. The treatments included UAV sprays at 30 and 45 L ha-1, as well as KMS at 500 L ha-1, with a weedy check as a control. Bispyribac-sodium 10% SC was applied at a rate of 35 g a.i ha-1 during the 2-to 3-leaf stage of the crops. The results revealed that droplet deposition, area coverage, and volume median diameter were affected by sprayer type (UAV and KMS). However, the two spray volumes tested using the UAV method provided similar droplet parameters. The KMS system at 500 L ha-1 exhibited the highest droplet deposition, while the UAV system achieved better droplet distribution at a spray volume of 30 L ha-1. Remarkably, the UAV system at 30 L ha-1 demonstrated effective weed control, which was statistically comparable to the KMS system at 500 L ha-1. These findings emphasize that the UAV spraying system with a spray volume of 30 L ha-1, delivering effective weed control while utilizing less carrier volume compared to the manual knapsack spraying method. Thus, the UAV spray system has great potential as a viable alternative to manual knapsack spraying for herbicide application in direct-seeded rice.

Introduction: India has the highest burden of animal bite cases globally, with a large proportion of the population affected by dog bites. India is endemic for rabies and accounts for 36% of the world’s rabies deaths. Mortality can be prevented by Post-Exposure Prophylaxis (PEP) with effective vaccines that are available. Vaccines are provided free of charge in Government hospitals. Aim: To estimate the compliance rate of Anti-Rabies Vaccine (ARV) regimen managed by the intradermal route. Materials and Methods: This was a cross-sectional record-based study from the ARV Register of Government Hospital, Gummidipoondi, Tamil Nadu, India. The victims of animal bite cases attending the hospital for the ARV regimen for a month were selected from the register to assess adherence to the full course updated Thai Red Cross regimen and identify any constraint factors hindering them from completing the course. Results: Out of 108 patients, 79 completed the full course ARV regimen, resulting in a compliance rate of 73.1%. Factors such as the distance of the hospital, timings, and forgotten dates were identified as major constraints. Conclusion: The compliance rate was found to be moderate, with constraint factors like forgotten dates and the distance of the hospital being significant hindrances to compliance. This highlights the importance of strengthening awareness through sensitisation programs and health education in both urban and rural areas to achieve a rabies-free India.

Introduction: Epilepsy is a serious psychological condition associated with social stigma, psychiatric co-morbidity, and a high economic burden. It is pertinent to develop novel treatment methods given the ever-evolving nature of the disease. Recently, various newer Antiepileptic Drugs (AEDs) have been under study, among which a newer targeted modality consists of SV2A receptors. Aim: To compare the antioxidant properties or free radical scavenging activity of the newer heterogeneous AEDs acting on the SV2A receptors, namely Levetiracetam (LEV) and Brivaracetam (BRV). Materials and Methods: This in-vitro study was conducted at Department of Pharmacology, Sri Ramachandra Institute of Higher Education and Research, Chennai, Tamil Nadu, India from July 2021 to August 2021. The study evaluated the antioxidant properties of LEV and BRV using various assays such as the Ferric Reducing Antioxidant Power (FRAP) test, DPPH (1,1-diphenyl-2-picrylhydrazyl) assay, Nitric Oxide (NO) radical scavenging assay, Superoxide Dismutase activity (SOD), and catalase activity. The obtained results were expressed using specific formulas related to the absorbance of the respective chemicals, along with the percentage of inhibition, and have been tabulated in Microsoft excel Sheet Version 16.16.27. Half-maximal Inhibitory Concentration (IC50) values were calculated using scatter plot graphs. Results: The FRAP values for LEV were reported to be significantly higher compared to BRV at concentrations of 20, 40, 60, 80 μg/mL, while at 100 μg/mL, BRV showed better ferric reducing activity. Although both drugs exhibited antioxidant activity, the results clearly identified LEV as a better NO radical scavenger, with a percentage of inhibition reaching up to 80.52%. During the SOD assay, the percentage inhibition of superoxide generation by LEV was found to be 99.02%, while that of BRV was 97.07% at a concentration of 100 μg/mL. LEV (0.573) also showed a higher degradation of H2O2 per minute than BRV (0.065) at a concentration of 100 μg/mL. Conclusion: The results showed that both drugs, BRV and LEV, exhibited significant antioxidant capacity. However, LEV demonstrated increased antioxidant potency and efficacy compared to BRV.

Research on sustainable consumer behavior has recently gained stream. An increasing number of academic articles seem to be focused on this subject. This study aims to identify and analyze the scientific literature using bibliometric analysis to identify the most cited articles, authors, and countries in the literature on consumer behavior in sustainable fashion. This study comprehensively investigates the topic by scrutinizing 759 publications between 1992 and 2024 sourced from the Scopus database. The study applied the VOSviewer to visualize and interpret the data. The study's findings are presented in a quantitative analysis with tables and maps. We analyzed an exponential increase in papers or research published on consumer behavior in sustainable fashion from 2012 to 2024. In addition to offering recommendations for future research directions, this paper provides significant insights that could influence firms' and regulators' policies and tactics targeted at encouraging sustainable fashion among consumers. [ABSTRACT FROM AUTHOR]

Introduction: Healthcare Professionals (HCPs) involved in managing Coronavirus Disease 2019 (COVID-19) pandemic were instructed to wear Personal Protective Equipment (PPE) to protect themselves from contracting virus. However, PPE use can sometimes lead to adverse events which create greater impact on health status of HCPs. Thus, the prevalence of adverse events and associated risk factors should be estimated for taking necessary preventive measures. Aim: To evaluate the prevalence of adverse events in HCPs due to PPE use during second wave of COVID-19 in Tamil Nadu, India. Materials and Methods: A cross-sectional study was conducted in different levels of healthcare centres in Tamil Nadu, India, from April to May 2021. Data were collected using a pre-validated questionnaire from HCPs of any discipline who were directly involved in managing COVID-19 patients. A total of 282 responses were collected through Google forms and proportion of HCPs who experienced adverse events due to PPE and percentage of different adverse events associated with PPE wearing were assessed. Data were analysed using Chi-square test. Results: Out of 282 respondents of the survey included 224 doctors, 34 nurses and 24 lab technicians with a mean age of 30 years. There were 164 females and 118 males. A total of 177 (62.76%) participants experienced adverse events which included dehydration, thirst and heat, headaches, inability to go to restroom and other urinary/respiratory problems. With respect to duration of exposure to PPE, 163 (57.8%) HCPs had >6 hours/day and 102 (36.2%) had 4-6 hours/day. It was observed that factors such as age, gender, profession, various wards posted for COVID-19 duty and duration of PPE worn daily were significantly associated with adverse events to PPE (p-value

Introduction: Neuroblastoma is the most common and earliest childhood tumour with complicated biological and clinical characteristics. The treatment includes chemotherapy, radiation therapy, surgical resection, stem cell therapy and many other modes, making the management difficult to tolerate and unacceptable. Thereby increasing the need to develop novel therapies or repurpose already existing ones with anticancer potential. Many studies have shown that vitamin D3 has anticancer properties. Vitamin D3 receptors have been found in neuroblastoma cell lines, according to research. Anticancer property of vitamin D3 hasn’t been studied much in neuroblastoma cell lines. Aim: To evaluate the antiproliferative and proapoptotic effects of vitamin D3 on human neuroblastoma cell lines-SH-SY5Y. Materials and Methods: The present study was an in-vitro study in which human neuroblastoma cell lines SY5Y (a total of 2 cell lines) were obtained from National Centre for Cell Science (NCCS), Pune, Maharashtra, India, and control cells are cells from the cell lines that were left untreated. The antiproliferative effect of vitamin D3 in human neuroblastoma cell lines evaluated using the MTT (3-(4,5-dimethylthiazolyl-2)-2,5 diphenyltetrazolium bromide) assay. After 48 hours of incubation and treatment with six different (0.01, 0.1, 1, 10, 100, and 1000 ng/mL) concentrations of vitamin D3, the percentage of viable cells was determined using spectrophotometry and compared with control cells (untreated cells from cell lines). Different vitamin D3 (250 ng/mL, 500 ng/mL, 1000 ng/mL) doses were applied to cells and they were then incubated for 24 hours, Cell death and malformations were then observed using a phase contrast microscope, and Deoxyribonucleic Acid (DNA) fragmentation was investigated using gel chromatography. The obtained results were expressed as percentage of inhibition and tabulated in Microsoft Excel Sheet Version 16.16.27 and scatter plot graph was used to calculate IC50 (Half maximal inhibitory concentration). Results: The vitamin D3 showed antiproliferative property in SH-SY5Y cells at an IC50 of 164 ng/mL when tested against human neuroblastoma cells using the MTT assay. Phase contrast microscope demonstrated that vitamin D3 treated cells showed condensation of nuclei, shrinkage of the cytoplasm, convolution of outline and cell peeling demonstrating apoptosis. DNA fragmentation also showed typical DNA ladder formation confirming apoptosis in vitamin D3 treated cells, which showed that the treated cells’ DNA was more damaged than the control cells’ DNA. Conclusion: Vitamin D3 exhibited both proapoptotic and antiproliferative properties, as demonstrated by the MTT assay, Phase Contrast, and DNA fragmentation.

Insecticide applications are typically being carried out with traditional manual spraying equipment in redgram, which leads to inadequate control of insects due to higher crop height. The modern deployment of tractor-drawn spray machines causes serious damage to the crop. In this connection, unmanned aerial vehicle (UAV) spray technology has great potential for precise insecticide application in redgram crops. One of the important machine parameters influencing droplet deposition and drift characteristics in UAV sprayers is downwash airflow generated by a multi-rotor propeller. A field experiment was carried out at the redgram research field (N11.01, E76.92), Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, during 2021–2022 to study the spray drift and deposition characteristics of an autonomous UAV sprayer. The Imidacloprid (a.i. 17.8SL) insecticide mixed with water in a ratio of 1 mL per liter was sprayed with a UAV sprayer. Water-sensitive paper samples were kept at upper, middle, and bottom positions on the leaves, and data were analyzed for the spray droplet size, deposition rate, droplet density, and area coverage both in target and non-target areas using Spray Deposit Scanner software. UAV spray droplet deposition rate (2.93 ± 0.17, 2.01 ± 0.08, and 2.21 ± 0.162.38 μL cm−2), droplet density (47 ± 4.04, 53 ± 3.61, and 52 ± 8.74 droplets cm−2), and area coverage (15.72 ± 0.39, 16.60 ± 0.71, and 14.99 ± 0.39%) were highest in the upper layer as compared to the middle layer (droplet deposition rate: 1.21 ± 0.08, 1.07 ± 0.03, and 0.77 ± 0.02 μL cm−2; droplet density: 42 ± 2.52, 43 ± 8.50, and 38 ± 2.52 droplets cm−2; area coverage: 10.95 ± 0.81, 11.22 ± 0.56, and 8.57 ± 0.44%) and bottom layer (droplet deposition rate: 0.41 ± 0.06, 0.35 ± 0.03, and 0.33 ± 0.03 μL cm−2; droplet density: 22 ± 4.36, 17 ± 3.51, and 19 ± 4.51 droplets cm−2; area coverage: 2.78 ± 0.29, 2.95 ± 0.45, and 2.46 ± 0.20%, respectively). In the spray drift test, there was a higher droplet deposition rate (1.63 ± 0.09, 1.93 ± 0.05, and 1.82 ± 0.06 μL cm−2), area coverage (14.40 ± 0.07, 17.54 ± 0.36, and 16.42 ± 0.30%), and droplet density (46 ± 3.61, 54 ± 2.08, and 45 ± 3.21 No’s cm−2) in the target area as compared to the non-target area (droplet deposition rate: 0.88 ± 0.02, 0.46 ± 0.03, 0.22 ± 0.05, and 0.00 μL cm−2; droplet density: 23 ± 1.53, 11 ± 2.08, 6 ± 1.53, and 0.00 droplets cm−2; area coverage: 7.58 ± 0.34, 4.41 ± 0.19, 2.16 ± 0.05, and 0.00%, respectively), which may have been due to the downwash airflow produced by the multi-rotor propeller of the UAV sprayer. Finally, the UAV-based spraying technology results showed that the downwash air produced by the six-rotor propeller improved the penetrability of insecticide to crop leaves and led to a higher droplet deposition rate, droplet density, area coverage, and droplet penetrability on the upper layer, middle layer, and bottom layer of the plants.

In recent times, the use of vertical take-off and landing (VTOL) multi-rotor Unmanned Aerial Vehicle (UAVs) for spraying chemical pesticides against weeds and pests has recently become popular. The current aerial spray application research is primarily focused on examining the influence of UAV spraying parameters such as flight height, travel speed, rotor configuration, droplet size, payload and wind velocity. The downwash airflow velocity generated by the UAV rotor propeller has a significant impact on the droplet deposition process. A test rig was developed to measure the downwash airflow pattern generated by the rotor propeller of a UAV. In this investigation, a six-rotor electric autonomous UAV sprayer was used to investigate the parameters and distribution laws of downwash airflow velocity. The downwash airflow velocity was measured using portable anemometers mounted on the test rig at radial positions viz., 0 m, 0.5 m, 1 m, 1.5 m and 2 m, perpendicular to (X) and parallel to the UAV’s flight direction (Y). The experiment was conducted at three levels of hover height, viz., 1 m, 2 m and 3 m (Z) and three levels of payload, viz., 0 kg, 5 kg, and 10 kg. The special downwash airflow distribution pattern was analysed using the Python programming language (Version 3.7). Results show that the downwash airflow velocity generated by the radial position of the UAV rotor is evenly distributed on the rotating loop and the standard deviation of the downwash airflow velocity is less than 0.5 m/s. The maximum downwash airflow velocity of 13.8 m/s was observed below the rotor at 10 kg payload capacity, 1 m hover height (Z), and 0.5 m in the X-direction. The minimum downwash wind field of 0.3 m/s was observed at 0 kg pay load capacity, 1 m height, and 2 m in the X-direction. The downwash airflow velocity along each position in the radial direction of the rotor increases initially and then decreases. This downwash airflow distribution results helps in mounting of spray nozzle configuration to drone sprayer which helps to understanding spray liquid distribution and other spray operational parameters. The influence of downwash airflow distribution combined with the spray operational parameters of the UAV sprayer viz., flight height, travel speed, rotor configuration, payload and wind velocity on spray volume distribution was studied. A field experiment was conducted to study the effect of UAV sprayer downwash airflow distribution on spray droplet deposition characteristics in a rice crop compared with manual knapsack sprayer. [ABSTRACT FROM AUTHOR]

Diabetes mellitus is a chronic disease which causes complications in a large population worldwide. Traditionally, medicinal plants possess numerous bioactive compounds to treat chronic diseases. In this study, the bioactive compounds in Cucumis pubescens Willd. were screened computationally to treat diabetes. Ten ligand molecules from C. pubescens Willd. plant fruit were selected from GC-MS analysis. The target proteins like Lipase-related Protein 2 (2OXE), α - amylase (2QV4) and β - Glucosidase (2ZOX) were retrieved from the PDB databank. The proteins have been selected based on their role in anti-diabetic activity. All ten ligands were docked with all three proteins to identify the suitable ligand possessing high binding energy. The ligand (4H-Pyran-4-one, 2,3-Dihydro 3,5-Dihydroxy-6-Methyl) had a maximum binding energy of -4.3 kcal/mol and -4.4 kcal/mol with the targets 2OXE and 2ZOX respectively. The ligand molecule 1,4-dimethyl-2,3-diazabicyclo[2.2.1]hept-2-ene produced the highest binding energy of -4.7 kcal/mol with 2QV4. The predicted active phytochemicals can be used as natural drug molecules to treat diabetes mellitus in future. [ABSTRACT FROM AUTHOR]

Featured Application: Porphyromonas gingivalis is an oral bacterium that uses gingipains as its primary invasion factor to cause periodontitis and certain systemic diseases in humans. To study the underlying mechanisms employed by gingipains, a simpler and quick approach is required to examine the protease activity. In the present study, passive diffusion-mediated protein elution and gelatin zymography methods were demonstrated to isolate and characterize the gingipains. This study found that an eluted protein with a molecular weight of about 180 kDa exhibited both Rgp and Kgp activity. While in the gelatin zymography, the proteins with a molecular weight of approximately 50 kDa, and above 245 kDa were found to be arginine-X gingipains when specific selective inhibitors, Leupeptin and Cathepsin B inhibitor II were used. The present study protocol will allow a more efficient separation of targeted protein samples with a greater difference in isoelectric point. Gingipains (RgpA, RgpB, and Kgp) are major virulence factors of the periodontitis-causing bacterium Porphyromonas gingivalis. Isolation of gingipains from the crude protein sample of P. gingivalis is critical for studying the underlying invasion mechanism that contributes to periodontitis, Alzheimer's disease, and cardiovascular disease (CVD). Chromatographic processes and molecular cloning are two standard techniques often used for gingipains isolation, which are time-consuming and costly. In this study, considerably easier methods based on passive-mediated diffusion gel elution and gelatin zymogram were used to isolate and characterize gingipains. Importantly, proteins eluted from Native-PAGE showed enzymatic activity for both Rgp and Kgp. In gelatin zymography, the proteins with a molecular size of ~50 kDa and above 245 kDa were suggested as arginine-specific gingipains. The passive diffusion-mediated gel elution method is a simpler technique to isolate gingipains from crude protein samples of P. gingivalis. By using covalent and highly specific gingipain inhibitors, gelatin zymography enabled an individual characterization of gingipain activity and inhibition. Finally, this protocol can be easily extended by adding the isoelectric focusing to further improve the protein separation and characterization. [ABSTRACT FROM AUTHOR]

The oral microbiome comprises several hundreds of bacterial species that contribute to periodontitis, the most complex polymicrobial inflammatory disorder. Porphyromonas gingivalis is a prominent periodontitis pathogen that produces gingipains as a major virulent factor. Gingipain facilitates P. gingivalis survival, pathogenicity, and growth. Several genes were identified to have a role in the regulating of P. gingivalis pathogenesis. Studies suggest that gingipains inhibition is key for the successful treatment of periodontitis. As of now, several gingipain inhibitors have been developed, some exhibit high inhibition activity against gingipains. However, most inhibitors offer unknown toxicity and undesirable side effects. Hence, the development of highly potent and safe gingipain inhibitor is a major concern for periodontitis treatment. The present review highlights the connectivity between P. gingivalis, virulent factors, and its gene, periodontitis, and gingipain inhibitors. Development of gingipains inhibitors would not only treat periodontitis but would also assist in the treatment of other associated systemic diseases, for example: rheumatoid arthritis, cardiovascular diseases, diabetes, and Alzheimer's disease. [ABSTRACT FROM AUTHOR]

Periodontal disease (PD) is complex polymicrobial disease which destroys tooth-supporting tissue. Although various synthetic inhibitors of periodontitis-triggering pathogens have been recognized, their undesirable side effects limit their application. Hence, the present study intended to perform the synthesis, characterization, antimicrobial evaluation, and cytotoxicity analysis of novel benzamidine analogues (NBA). This study involved the synthesis of novel imino bases of benzamidine (4a–c), by reacting different aromatic aldehydes with 2-(4-carbamimidoylphenoxy) acetohydrazide (3), which was synthesized by the hydrazination of ethyl 2-(4-carbamimidoylphenoxy) acetate (2), the derivative of 4-hydroxybenzene carboximidamide (1). This was followed by characterization using FTIR, 1H, 13C NMR and mass spectrometry. All synthesized compounds were further tested for antimicrobial potential against PD-triggering pathogens by the micro broth dilution method. The cytotoxicity analysis of the NBA against HEK 293 cells was conducted using an MTT assay. The present study resulted in a successful synthesis of NBA and elucidated their structures. The synthesized NBA exhibited significant antimicrobial activity values between 31.25 and 125 µg/mL against tested pathogens. All NBA exhibited weak cytotoxicity against HEK 293 cells at 7.81 µg, equally to chlorhexidine at 0.2%. The significant antimicrobial activity of NBA against PD-triggering pathogens supports their potential application in periodontitis treatment. [ABSTRACT FROM AUTHOR]

Hypoxia-inducible factor-1 (HIF-1) plays crucial roles in tumor promotion by upregulating its target genes, which are involved in energy metabolism, angiogenesis, cell survival, invasion, metastasis, and drug resistance. The HIF-1alpha subunit, which is regulated by O2-dependent hydroxylation, ubiquitination, and degradation, has been identified as an important molecular target for cancer therapy. We have rationally designed G-rich oligodeoxynucleotides (ODNs) as inhibitors of HIF-1alpha for human cancer therapy. The lead compounds, JG243 and JG244, which form an intramolecular parallel G-quartet structure, selectively target HIF-1alpha and decreased levels of both HIF-1alpha and HIF-2alpha (IC50 < 2 micromol/l) and also inhibited the expression of HIF-1-regulated proteins [vascular endothelial growth factor (VEGF), Bcl-2, and Bcl-XL], but did not disrupt the expression of p300, Stat3, or p53. JG-ODNs induced proteasomal degradation of HIF-1alpha and HIF-2alpha that was dependent on the hydroxylase activity of prolyl-4-hydroxylase-2. JG243 and JG244 dramatically suppressed the growth of prostate, breast, and pancreatic tumor xenografts. Western blots from tumor tissues showed that JG-ODNs significantly decreased HIF-1alpha and HIF-2alpha levels and blocked the expression of VEGF. The JG-ODNs are novel anticancer agents that suppress tumor growth by inhibiting HIF-1.