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137 results on '"Kaustov, Lilia"'

5. Long-term Outcomes with Spinal versus General Anesthesia for Hip Fracture Surgery: A Randomized Trial

Author

Vail, Emily A., Feng, Rui, Sieber, Frederick, Carson, Jeffrey L., Ellenberg, Susan S., Magaziner, Jay, Dillane, Derek, Marcantonio, Edward R., Sessler, Daniel I., Ayad, Sabry, Stone, Trevor, Papp, Steven, Donegan, Derek, Mehta, Samir, Schwenk, Eric S., Marshall, Mitchell, Jaffe, J. Douglas, Luke, Charles, Sharma, Balram, Azim, Syed, Hymes, Robert, Chin, Ki-Jinn, Sheppard, Richard, Perlman, Barry, Sappenfield, Joshua, Hauck, Ellen, Tierney, Ann, Horan, Annamarie D., Neuman, Mark D., Looke, Thomas, Bent, Sandra, Franco-Mora, Ariana, Hedrick, Pamela, Newbern, Matthew, Tadros, Rafik, Pealer, Karen, Vlassakov, Kamen, Buckley, Carolyn, Gavin, Lauren, Gorbatov, Svetlana, Gosnell, James, Steen, Talora, Vafai, Avery, Zeballos, Jose, Hruslinski, Jennifer, Cardenas, Louis, Berry, Ashley, Getchell, John, Quercetti, Nicholas, Hassan, Manal, Bajracharya, Gauasan, Billow, Damien, Bloomfield, Michael, Cuko, Evis, Elyaderani, Mehrun K., Hampton, Robert, Honar, Hooman, Khoshknabi, Dilara, Kim, Daniel, Krahe, David, Lew, Michael M., Maheshwer, Conjeevram B., Niazi, Azfar, Saha, Partha, Salih, Ahmed, de Swart, Robert J., Volio, Andrew, Bolkus, Kelly, DeAngelis, Matthew, Dodson, Gregory, Gerritsen, Jeffrey, McEniry, Brian, Mitrev, Ludmil, Kwofie, M. Kwesi, Belliveau, Anne, Bonazza, Flynn, Lloyd, Vera, Panek, Izabela, Dabiri, Jared, Chavez, Chris, Craig, Jason, Davidson, Todd, Dietrichs, Chad, Fleetwood, Cheryl, Foley, Mike, Getto, Chris, Hailes, Susie, Hermes, Sarah, Hooper, Andy, Koener, Greg, Kohls, Kate, Law, Leslie, Lipp, Adam, Losey, Allison, Nelson, William, Nieto, Mario, Rogers, Pam, Rutman, Steve, Scales, Garrett, Sebastian, Barbara, Stanciu, Tom, Lobel, Gregg, Giampiccolo, Michelle, Herman, Dara, Kaufman, Margit, Murphy, Bryan, Pau, Clara, Puzio, Thomas, Veselsky, Marlene, Apostle, Kelly, Boyer, Dory, Chen Fan, Brenda, Lee, Susan, Lemke, Mike, Merchant, Richard, Moola, Farhad, Payne, Kyrsten, Perey, Bertrand, Viskontas, Darius, Poler, Mark, D’Antonio, Patricia, O’Neill, Greg, Abdullah, Amer, Fish-Fuhrmann, Jamie, Giska, Mark, Fidkowski, Christina, Guthrie, Stuart Trent, Hakeos, William, Hayes, Lillian, Hoegler, Joseph, Nowak, Katherine, Beck, Jeffery, Cuff, Jaslynn, Gaski, Greg, Haaser, Sharon, Holzman, Michael, Malekzadeh, A. Stephen, Ramsey, Lolita, Schulman, Jeff, Schwartzbach, Cary, Azefor, Tangwan, Davani, Arman, Jaberi, Mahmood, Masear, Courtney, Haider, Syed Basit, Chungu, Carolyn, Ebrahimi, Ali, Fikry, Karim, Marcantonio, Andrew, Shelvan, Anitha, Sanders, David, Clarke, Collin, Lawendy, Abdel, Schwartz, Gary, Garg, Mohit, Kim, Joseph, Caruci, Juan, Commeh, Ekow, Cuevas, Randy, Cuff, Germaine, Franco, Lola, Furgiuele, David, Giuca, Matthew, Allman, Melissa, Barzideh, Omid, Cossaro, James, D’Arduini, Armando, Farhi, Anita, Gould, Jason, Kafel, John, Patel, Anuj, Peller, Abraham, Reshef, Hadas, Safur, Mohammed, Toscano, Fiore, Tedore, Tiffany, Akerman, Michael, Brumberger, Eric, Clark, Sunday, Friedlander, Rachel, Jegarl, Anita, Lane, Joseph, Lyden, John P., Mehta, Nili, Murrell, Matthew T., Painter, Nathan, Ricci, William, Sbrollini, Kaitlyn, Sharma, Rahul, Steel, Peter A.D., Steinkamp, Michele, Weinberg, Roniel, Stephenson Wellman, David, Nader, Antoun, Fitzgerald, Paul, Ritz, Michaela, Bryson, Greg, Craig, Alexandra, Farhat, Cassandra, Gammon, Braden, Gofton, Wade, Harris, Nicole, Lalonde, Karl, Liew, Allan, Meulenkamp, Bradley, Sonnenburg, Kendra, Wai, Eugene, Wilkin, Geoffrey, Troxell, Karen, Alderfer, Mary Ellen, Brannen, Jason, Cupitt, Christopher, Gerhart, Stacy, McLin, Renee, Sheidy, Julie, Yurick, Katherine, Chen, Fei, Dragert, Karen, Kiss, Geza, Malveaux, Halina, McCloskey, Deborah, Mellender, Scott, Mungekar, Sagar S., Noveck, Helaine, Sagebien, Carlos, Biby, Luat, McKelvy, Gail, Richards, Anna, Abola, Ramon, Ayala, Brittney, Halper, Darcy, Mavarez, Ana, Rizwan, Sabeen, Choi, Stephen, Awad, Imad, Flynn, Brendan, Henry, Patrick, Jenkinson, Richard, Kaustov, Lilia, Lappin, Elizabeth, McHardy, Paul, Singh, Amara, Donnelly, Joanne, Gonzalez, Meera, Haydel, Christopher, Livelsberger, Jon, Pazionis, Theresa, Slattery, Bridget, Vazquez-Trejo, Maritza, Baratta, Jaime, Cirullo, Michael, Deiling, Brittany, Deschamps, Laura, Glick, Michael, Katz, Daniel, Krieg, James, Lessin, Jennifer, Mojica, Jeffrey, Torjman, Marc, Jin, Rongyu, Salpeter, Mary Jane, Powell, Mark, Simmons, Jeffrey, Lawson, Prentiss, Kukreja, Promil, Graves, Shanna, Sturdivant, Adam, Bryant, Ayesha, Crump, Sandra Joyce, Verrier, Michelle, Green, James, Menon, Matthew, Applegate, Richard, Arias, Ana, Pineiro, Natasha, Uppington, Jeffrey, Wolinsky, Phillip, Gunnett, Amy, Hagen, Jennifer, Harris, Sara, Hollen, Kevin, Holloway, Brian, Horodyski, Mary Beth, Pogue, Trevor, Ramani, Ramachandran, Smith, Cameron, Woods, Anna, Warrick, Matthew, Flynn, Kelly, Mongan, Paul, Ranganath, Yatish, Fernholz, Sean, Ingersoll-Weng, Esperanza, Marian, Anil, Seering, Melinda, Sibenaller, Zita, Stout, Lori, Wagner, Allison, Walter, Alicia, Wong, Cynthia, Orwig, Denise, Goud, Maithri, Helker, Chris, Mezenghie, Lydia, Montgomery, Brittany, Preston, Peter, Schwartz, J. Sanford, Weber, Ramona, Fleisher, Lee A., Mehta, Samir, Stephens-Shields, Alisa J., Dinh, Cassandra, Schwartz, Aron, Chelly, Jacques E., Goel, Shiv, Goncz, Wende, Kawabe, Touichi, Khetarpal, Sharad, Monroe, Amy, Shick, Vladislav, Breidenstein, Max, Dominick, Timothy, Friend, Alexander, Mathews, Donald, Lennertz, Richard, Sanders, Robert, Akere, Helen, Balweg, Tyler, Bo, Amber, Doro, Christopher, Goodspeed, David, Lang, Gerald, Parker, Maggie, Rettammel, Amy, Roth, Mary, White, Marissa, Whiting, Paul, Allen, Brian F.S., Baker, Tracie, Craven, Debra, McEvoy, Matt, Turnbo, Teresa, Kates, Stephen, Morgan, Melanie, Willoughby, Teresa, Weigel, Wade, Auyong, David, Fox, Ellie, Welsh, Tina, Cusson, Bruce, Dobson, Sean, Edwards, Christopher, Harris, Lynette, Henshaw, Daryl, Johnson, Kathleen, McKinney, Glen, Miller, Scott, Reynolds, Jon, Segal, B. Scott, Turner, Jimmy, VanEenenaam, David, Weller, Robert, Lei, Jineli, Treggiari, Miriam, Akhtar, Shamsuddin, Blessing, Marcelle, Johnson, Chanel, Kampp, Michael, Kunze, Kimberly, OʼConnor, Mary, Gaskins, Lakisha J., Looke, Thomas, Tadros, Rafik, Vlassakov, Kamen, Cardenas, Louis, Hassan, Manal, Bolkus, Kelly, Mitrev, Ludmil, Kwofie, M. Kwesi, Dabiri, Jared, Lobel, Gregg, Poler, Mark, Giska, Mark, Sanders, David, Schwartz, Gary, Giuca, Matthew, Tedore, Tiffany, Nader, Antoun, Papp, Stephen, Bryson, Greg, Troxell, Karen, Kiss, Geza, Choi, Stephen, Powell, Mark, Applegate, Richard, Warrick, Matthew, Ranganath, Yatish, Elkassabany, Nabil, Chelly, Jacques E., Hoeft, Mark A., Lennertz, Richard, Sanders, Robert, Allen, Brian F.S., Kates, Stephen, Weigel, Wade, Li, Jinlei, Wijeysundera, Duminda N., Kheterpal, Sachin, Moore, Reneé H., Smith, Alexander K., Tosi, Laura L., Elkassabany, Nabil, Looke, Thomas, Menio, Diane, Mehta, Samir, Fleisher, Lee, Menio, Diane, Hruslinski, Jennifer, Ramsey, Lolita, Gaskins, Lakisha J., Langlois, Christine, Gaskins, Lakisha J., Mezenghie, Lydia, Montgomery, Brittany, Oduwole, Samuel, and Rose, Thomas

Published
2024
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7. Age and its impact on crisis management performance and learning after simulation-based education by acute care physicians: a multicentre prospective cohort study

Author

Alam, Fahad, LeBlanc, Vicki R., Baxter, Alan, Tarshis, Jordan, Piquette, Dominique, Gu, Yuqi, Filipowska, Caroline, Krywenky, Ashley, Kester-Greene, Nicole, Cardinal, Pierre, Andrews, Meghan, Chartier, Francois, Burrows, Claire, Houzé-Cerfon, Charles-Henri, Burns, Joseph K., Kaustov, Lilia, Au, Shelly, Lam, Sandy, DeSousa, Susan, and Boet, Sylvain

Published
2023
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8. Engaging patients as partners in a multicentre trial of spinal versus general anaesthesia for older adults

Author

Looke, Thomas, Bent, Sandra, Franco-Mora, Ariana, Hedrick, Pamela, Newbern, Matthew, Tadros, Rafik, Pealer, Karen, Marcantonio, Edward, Vlassakov, Kamen, Buckley, Carolyn, Gorbatov, Svetlana, Gosnell, James, Steen, Talora, Vafai, Avery, Zeballos, Jose, Cardenas, Louis, Berry, Ashley, Getchell, John, Quercetti, Nicholas, Sessler, Daniel I., Ayad, Sabry, Hassan, Manal, Ali, Assad, Bajracharya, Gauasan, Billow, Damien, Bloomfield, Michael, Elliott, Kavita, Hampton, Robert, He, Linda, Honar, Hooman, Khoshknabi, Dilara, Kim, Daniel, Minko, Paul, Morris, Adam, Niazi, Azfar, Nutcharoen, Tara, Roberts, Jeffrey, Saha, Partha, Salih, Ahmed, Skolaris, Alexis, Stang, Taylor, Strimbu, Victor, Templeton, Jesse, Volio, Andrew, Wang, Jiayi, Bolkus, Kelly, DeAngelis, Matthew, Dodson, Gregory, Gerritsen, Jeffrey, McEniry, Brian, Mitrev, Ludmil, Kwofie, Kwesi, Bonazza, Flynn, Lloyd, Vera, Panek, Izabela, Dabiri, Jared, Chavez, Chris, Craig, Jason, Davidson, Todd, Dietrichs, Chad, Fleetwood, Cheryl, Foley, Mike, Getto, Chris, Hailes, Susie, Hermes, Sarah, Hooper, Andy, Koener, Greg, Kohls, Kate, Law, Leslie, Lipp, Adam, Losey, Allison, Nelson, William, Nieto, Mario, Rogers, Pam, Rutman, Steve, Scales, Garrett, Sebastian, Barbara, Stanciu, Tom, Lobel, Gregg, Giampiccolo, Michelle, Herman, Dara, Kaufman, Margit, Murphy, Bryan, Pau, Clara, Puzio, Thomas, Veselsky, Marlene, Stone, Trevor, Apostle, Kelly, Boyer, Dory, Fan, Brenda Chen, Lee, Susan, Lemke, Mike, Merchant, Richard, Moola, Farhad, Payne, Kyrsten, Perey, Bertrand, Viskontas, Darius, Poler, Mark, D'Antonio, Patricia, Sheppard, Richard, Abdullah, Amer, Fish-Fuhrmann, Jamie, Giska, Mark, Fidkowski, Christina, Guthrie, Trent, Hakeos, William, Hayes, Lillian, Hoegler, Joseph, Nowak, Katherine, Hymes, Robert, Beck, Jeffery, Cuff, Jaslynn, Gaski, Greg, Haaser, Sharon, Holzman, Michael, Malekzadeh, A. Stephen, Ramsey, Lolita, Schulman, Jeff, Schwartzbach, Cary, Sieber, Frederick, Azefor, Tangwan, Brown, Charles, Davani, Arman, Jaberi, Mahmood, Masear, Courtney, Sharma, Balram, Haider, Syed Basit, Chungu, Carolyn, Ebrahimi, Ali, Fikry, Karim, Gannon, Kerri, Marcantonio, Andrew, Pace, Meredith, Sanders, David, Clarke, Collin, Lawendy, Abdel, Schwartz, Gary, Garg, Mohit, Kim, Joseph, Marshall, Mitchell, Caurci, Juan, Commeh, Ekow, Cuevas, Randy, Cuff, Germaine, Franco, Lola, Furguiele, David, Giuca, Matthew, Allman, Melissa, Barzideh, Omid, Cossaro, James, D'Arduini, Armando, Farhi, Anita, Gould, Jason, Kafel, John, Patel, Anuj, Peller, Abraham, Reshef, Hadas, Safur, Mohammed, Toscano, Fiore, Tedore, Tiffany, Akerman, Michael, Brumberger, Eric, Clark, Sunday, Friedlander, Rachel, Jegarl, Anita, Lane, Joseph, Lyden, John P., Mehta, Nili, Murrell, Matthew T., Painter, Nathan, Ricci, William, Sbrollini, Kaitlyn, Sharma, Rahul, Steel, Peter A.D., Steinkamp, Michele, Weinberg, Roniel, Wellman, David Stephenson, Nader, Antoun, Fitzgerald, Paul, Ritz, Michaela, Papp, Steven, Bryson, Greg, Craig, Alexandra, Farhat, Cassandra, Gammon, Braden, Gofton, Wade, Harris, Nicole, Lalonde, Karl, Liew, Allan, Meulenkamp, Bradley, Sonnenburg, Kendra, Wai, Eugene, Wilkin, Geoffrey, Donegan, Derek, Dinh, Cassandra, Elkassabany, Nabil, Horan, Annamarie, Mehta, Samir, Troxell, Karen, Alderfer, Mary Ellen, Brannen, Jason, Cupitt, Christopher, Gerhart, Stacy, McLin, Renee, Sheidy, Julie, Yurick, Katherine, Carson, Jeffrey, Chen, Fei, Dragert, Karen, Kiss, Geza, Malveaux, Halina, McCloskey, Deborah, Mellender, Scott, Mungekar, Sagar S., Noveck, Helaine, Sagebien, Carlos, Perlman, Barry, Biby, Luat, McKelvy, Gail, Richards, Anna, Azim, Syed, Abola, Ramon, Ayala, Brittney, Halper, Darcy, Mavarez, Ana, Choi, Stephen, Awad, Imad, Flynn, Brendan, Henry, Patrick, Jenkinson, Richard, Kaustov, Lilia, Lappin, Elizabeth, McHardy, Paul, Singh, Amara, Hauck, Ellen, Donnelly, Joanne, Gonzalez, Meera, Haydel, Christopher, Livelsberger, Jon, Pazionis, Theresa, Slattery, Bridget, Vazquez-Trejo, Maritza, Schwenk, Eric, Baratta, Jaime, Deiling, Brittany, Deschamps, Laura, Glick, Michael, Katz, Daniel, Krieg, James, Lessin, Jennifer, Torjman, Marc, Chin, Ki Jinn, Jin, Rongyu, Salpeter, Mary Jane, Powell, Mark, Simmons, Jeffrey, Lawson, Prentiss, Kukreja, Promil, Graves, Shanna, Sturdivant, Adam, Bryant, Ayesha, Crump, Sandra Joyce, Dillane, Derek, Taylor, Michael, Verrier, Michelle, Applegate, Richard, Arias, Ana, Pineiro, Natasha, Uppington, Jeffrey, Wolinsky, Phillip, Sappenfield, Joshua, Gunnett, Amy, Hagen, Jennifer, Harris, Sara, Hollen, Kevin, Holloway, Brian, Horodyski, Mary Beth, Pogue, Trevor, Ramani, Ramachandran, Smith, Cameron, Woods, Anna, Warrick, Matthew, Flynn, Kelly, Mongan, Paul, Ranganath, Yatish, Fernholz, Sean, Ingersoll-Weng, Esperanza, Marian, Anil, Seering, Melinda, Sibenaller, Zita, Stout, Lori, Wagner, Allison, Walter, Alicia, Wong, Cynthia, Magaziner, Jay, Orwig, Denise, Brown, Trina, Dattilo, Jim, Ellenberg, Susan, Feng, Rui, Fleisher, Lee, Gaskins, Lakisha, Goud, Maithri, Helker, Chris, Mezenghie, Lydia, Montgomery, Brittany, Preston, Peter, Stephens, Alisa, Schwartz, J. Sanford, Tierney, Ann, Weber, Ramona, Chelly, Jacques, Goel, Shiv, Goncz, Wende, Kawabe, Touichi, Khetarpal, Sharad, King, Kevin, Kunkel, Frank, Luke, Charles, Monroe, Amy, Shick, Vladislav, Silipo, Anthony, Stehle, Caroline, Szabo, Katherine, Yennam, Sudhakar, Hoeft, Mark, Breidenstein, Max, Dominick, Timothy, Friend, Alexander, Mathews, Donald, Lennertz, Richard, Akere, Helen, Balweg, Tyler, Bo, Amber, Doro, Christopher, Goodspeed, David, Lang, Gerald, Parker, Maggie, Rettammel, Amy, Roth, Mary, Sanders, Robert, White, Marissa, Whiting, Paul, Allen, Brian, Baker, Tracie, Craven, Debra, McEvoy, Matt, Turnbo, Teresa, Kates, Stephen, Morgan, Melanie, Willoughby, Teresa, Weigel, Wade, Auyong, David, Fox, Ellie, Welsh, Tina, Jaffe, J. Douglas, Cusson, Bruce, Dobson, Sean, Edwards, Christopher, Harris, Lynette, Henshaw, Daryl, Johnson, Kathleen, McKinney, Glen, Miller, Scott, Reynolds, Jon, Turner, Jimmy, VanEenenaam, David, Weller, Robert, Akhtar, Shamsuddin, Blessing, Marcelle, Johnson, Chanel, Kampp, Michael, Kunze, Kimberly, Li, Jinlei, O'Connor, Mary, Treggiari, Miriam, Hruslinski, Jennifer, Menio, Diane A., Hymes, Robert A., Langlois, Christine, Gaskins, Lakisha J., and Neuman, Mark D.

Published
2021
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13. Role of Pirh2 in mediating the regulation of p53 and c-Myc.

Author

Hakem, Anne, Bohgaki, Miyuki, Lemmers, Bénédicte, Tai, Elisabeth, Salmena, Leonardo, Matysiak-Zablocki, Elzbieta, Jung, Yong-Sam, Karaskova, Jana, Kaustov, Lilia, Duan, Shili, Madore, Jason, Boutros, Paul, Sheng, Yi, Chesi, Marta, Bergsagel, P Leif, Perez-Ordonez, Bayardo, Mes-Masson, Anne-Marie, Penn, Linda, Squire, Jeremy, Chen, Xinbin, Jurisica, Igor, Arrowsmith, Cheryl, Sanchez, Otto, Benchimol, Samuel, and Hakem, Razqallah

Subjects
Animals, Mice, Inbred C57BL, Humans, Mice, Neoplasms, Cell Transformation, Neoplastic, Ubiquitin-Protein Ligases, Proto-Oncogene Proteins c-myc, Gene Expression Regulation, Neoplastic, Genes, Tumor Suppressor, Radiation Tolerance, Tumor Suppressor Protein p53, Ubiquitination, Kaplan-Meier Estimate, HEK293 Cells, Proteolysis, Inbred C57BL, Cell Transformation, Neoplastic, Gene Expression Regulation, Genes, Tumor Suppressor, Genetics, Developmental Biology
Abstract

Ubiquitylation is fundamental for the regulation of the stability and function of p53 and c-Myc. The E3 ligase Pirh2 has been reported to polyubiquitylate p53 and to mediate its proteasomal degradation. Here, using Pirh2 deficient mice, we report that Pirh2 is important for the in vivo regulation of p53 stability in response to DNA damage. We also demonstrate that c-Myc is a novel interacting protein for Pirh2 and that Pirh2 mediates its polyubiquitylation and proteolysis. Pirh2 mutant mice display elevated levels of c-Myc and are predisposed for plasma cell hyperplasia and tumorigenesis. Consistent with the role p53 plays in suppressing c-Myc-induced oncogenesis, its deficiency exacerbates tumorigenesis of Pirh2(-/-) mice. We also report that low expression of human PIRH2 in lung, ovarian, and breast cancers correlates with decreased patients' survival. Collectively, our data reveal the in vivo roles of Pirh2 in the regulation of p53 and c-Myc stability and support its role as a tumor suppressor.

Published
2011

14. Supplementary Figures 1-5 from CUL7 Is a Novel Antiapoptotic Oncogene

Author

Kim, Sam S., primary, Shago, Mary, primary, Kaustov, Lilia, primary, Boutros, Paul C., primary, Clendening, James W., primary, Sheng, Yi, primary, Trentin, Grace A., primary, Barsyte-Lovejoy, Dalia, primary, Mao, Daniel Y.L., primary, Kay, Robert, primary, Jurisica, Igor, primary, Arrowsmith, Cheryl H., primary, and Penn, Linda Z., primary

Published
2023
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15. Supplementary Figure Legends 1-5 from CUL7 Is a Novel Antiapoptotic Oncogene

Author

Kim, Sam S., primary, Shago, Mary, primary, Kaustov, Lilia, primary, Boutros, Paul C., primary, Clendening, James W., primary, Sheng, Yi, primary, Trentin, Grace A., primary, Barsyte-Lovejoy, Dalia, primary, Mao, Daniel Y.L., primary, Kay, Robert, primary, Jurisica, Igor, primary, Arrowsmith, Cheryl H., primary, and Penn, Linda Z., primary

Published
2023
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16. Data from CUL7 Is a Novel Antiapoptotic Oncogene

Author

Kim, Sam S., primary, Shago, Mary, primary, Kaustov, Lilia, primary, Boutros, Paul C., primary, Clendening, James W., primary, Sheng, Yi, primary, Trentin, Grace A., primary, Barsyte-Lovejoy, Dalia, primary, Mao, Daniel Y.L., primary, Kay, Robert, primary, Jurisica, Igor, primary, Arrowsmith, Cheryl H., primary, and Penn, Linda Z., primary

Published
2023
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18. Abstract 844: Germline TP53 missense mutations at codon338 confer adult-onset cancer risk through weakened p53 protein oligomeric stability that impairs its tumor suppressive mechanisms

Author

Fischer, Nicholas W., primary, Turk, Jaime, additional, Ong, Noel, additional, Duan, Shili, additional, Kaustov, Lilia, additional, Kratz, Christian, additional, Pajtler, Kristian, additional, Kim, Raymond, additional, Arrowsmith, Cheryl, additional, and Malkin, David, additional

Published
2022
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19. Creating a Successful Virtual Reality-Based Medical Simulation Environment: Tutorial.

Author

Gupta, Sanchit, Wilcocks, Kyle, Matava, Clyde, Wiegelmann, Julian, Kaustov, Lilia, and Alam, Fahad

Subjects
VIRTUAL reality, MEDICAL education, MEDICAL simulation, MEDICAL innovations, CURRICULUM
Abstract

Innovation in medical education is not only inevitable but a requirement. Manikin-based simulation is currently the gold standard for supplemental clinical training; however, this modality requires significant equipment and personnel to operate. Virtual reality (VR) is emerging as a new method of delivering medical simulation sessions that requires less infrastructure but also allows for greater accessibility and flexibility. VR has slowly been integrated into the medical curriculum in some hospitals; however, more widespread adoption would transform the delivery of medical education for future clinicians. This tutorial introduces educators to the BUILD REALITY (begin, use, identify, leverage, define, recreate, educate, adapt, look, identify, test, amplify) framework, a series of practical tips for designing and implementing a VR-based medical simulation environment in their curriculum. The suggestions are based on the relevant literature and the authors' personal experience in creating and implementing VR environments for medical trainees. Altogether, this paper provides guidance on conducting a needs assessment, setting objectives, designing a VR environment, and incorporating the session into the broader medical curriculum. [ABSTRACT FROM AUTHOR]

Published
2023
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22. Cognitive Outcomes after DEXmedetomidine sedation in cardiac surgery: CODEX randomised controlled trial protocol

Author

Choi, Stephen, primary, Jerath, Angela, additional, Jones, Philip, additional, Avramescu, Sinziana, additional, Djaiani, George, additional, Syed, Summer, additional, Saha, Tarit, additional, Kaustov, Lilia, additional, Kiss, Alex, additional, D'Aragon, Frédérick, additional, Hedlin, Peter, additional, Rajamohan, Raja, additional, Couture, Etienne J., additional, Singh, Amara, additional, Mapplebeck, Josiane CS, additional, Wong, Sophia, additional, and Orser, Beverley Anne, additional

Published
2021
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24. Engaging patients as partners in a multicentre trial of spinal versus general anaesthesia for older adults

Author

Hruslinski, Jennifer, primary, Menio, Diane A., additional, Hymes, Robert A., additional, Jaffe, J. Douglas, additional, Langlois, Christine, additional, Ramsey, Lolita, additional, Gaskins, Lakisha J., additional, Neuman, Mark D., additional, Looke, Thomas, additional, Bent, Sandra, additional, Franco-Mora, Ariana, additional, Hedrick, Pamela, additional, Newbern, Matthew, additional, Tadros, Rafik, additional, Pealer, Karen, additional, Marcantonio, Edward, additional, Vlassakov, Kamen, additional, Buckley, Carolyn, additional, Gorbatov, Svetlana, additional, Gosnell, James, additional, Steen, Talora, additional, Vafai, Avery, additional, Zeballos, Jose, additional, Cardenas, Louis, additional, Berry, Ashley, additional, Getchell, John, additional, Quercetti, Nicholas, additional, Sessler, Daniel I., additional, Ayad, Sabry, additional, Hassan, Manal, additional, Ali, Assad, additional, Bajracharya, Gauasan, additional, Billow, Damien, additional, Bloomfield, Michael, additional, Elliott, Kavita, additional, Hampton, Robert, additional, He, Linda, additional, Honar, Hooman, additional, Khoshknabi, Dilara, additional, Kim, Daniel, additional, Minko, Paul, additional, Morris, Adam, additional, Niazi, Azfar, additional, Nutcharoen, Tara, additional, Roberts, Jeffrey, additional, Saha, Partha, additional, Salih, Ahmed, additional, Skolaris, Alexis, additional, Stang, Taylor, additional, Strimbu, Victor, additional, Templeton, Jesse, additional, Volio, Andrew, additional, Wang, Jiayi, additional, Bolkus, Kelly, additional, DeAngelis, Matthew, additional, Dodson, Gregory, additional, Gerritsen, Jeffrey, additional, McEniry, Brian, additional, Mitrev, Ludmil, additional, Kwofie, Kwesi, additional, Bonazza, Flynn, additional, Lloyd, Vera, additional, Panek, Izabela, additional, Dabiri, Jared, additional, Chavez, Chris, additional, Craig, Jason, additional, Davidson, Todd, additional, Dietrichs, Chad, additional, Fleetwood, Cheryl, additional, Foley, Mike, additional, Getto, Chris, additional, Hailes, Susie, additional, Hermes, Sarah, additional, Hooper, Andy, additional, Koener, Greg, additional, Kohls, Kate, additional, Law, Leslie, additional, Lipp, Adam, additional, Losey, Allison, additional, Nelson, William, additional, Nieto, Mario, additional, Rogers, Pam, additional, Rutman, Steve, additional, Scales, Garrett, additional, Sebastian, Barbara, additional, Stanciu, Tom, additional, Lobel, Gregg, additional, Giampiccolo, Michelle, additional, Herman, Dara, additional, Kaufman, Margit, additional, Murphy, Bryan, additional, Pau, Clara, additional, Puzio, Thomas, additional, Veselsky, Marlene, additional, Stone, Trevor, additional, Apostle, Kelly, additional, Boyer, Dory, additional, Fan, Brenda Chen, additional, Lee, Susan, additional, Lemke, Mike, additional, Merchant, Richard, additional, Moola, Farhad, additional, Payne, Kyrsten, additional, Perey, Bertrand, additional, Viskontas, Darius, additional, Poler, Mark, additional, D'Antonio, Patricia, additional, Sheppard, Richard, additional, Abdullah, Amer, additional, Fish-Fuhrmann, Jamie, additional, Giska, Mark, additional, Fidkowski, Christina, additional, Guthrie, Trent, additional, Hakeos, William, additional, Hayes, Lillian, additional, Hoegler, Joseph, additional, Nowak, Katherine, additional, Hymes, Robert, additional, Beck, Jeffery, additional, Cuff, Jaslynn, additional, Gaski, Greg, additional, Haaser, Sharon, additional, Holzman, Michael, additional, Malekzadeh, A. Stephen, additional, Schulman, Jeff, additional, Schwartzbach, Cary, additional, Sieber, Frederick, additional, Azefor, Tangwan, additional, Brown, Charles, additional, Davani, Arman, additional, Jaberi, Mahmood, additional, Masear, Courtney, additional, Sharma, Balram, additional, Haider, Syed Basit, additional, Chungu, Carolyn, additional, Ebrahimi, Ali, additional, Fikry, Karim, additional, Gannon, Kerri, additional, Marcantonio, Andrew, additional, Pace, Meredith, additional, Sanders, David, additional, Clarke, Collin, additional, Lawendy, Abdel, additional, Schwartz, Gary, additional, Garg, Mohit, additional, Kim, Joseph, additional, Marshall, Mitchell, additional, Caurci, Juan, additional, Commeh, Ekow, additional, Cuevas, Randy, additional, Cuff, Germaine, additional, Franco, Lola, additional, Furguiele, David, additional, Giuca, Matthew, additional, Allman, Melissa, additional, Barzideh, Omid, additional, Cossaro, James, additional, D'Arduini, Armando, additional, Farhi, Anita, additional, Gould, Jason, additional, Kafel, John, additional, Patel, Anuj, additional, Peller, Abraham, additional, Reshef, Hadas, additional, Safur, Mohammed, additional, Toscano, Fiore, additional, Tedore, Tiffany, additional, Akerman, Michael, additional, Brumberger, Eric, additional, Clark, Sunday, additional, Friedlander, Rachel, additional, Jegarl, Anita, additional, Lane, Joseph, additional, Lyden, John P., additional, Mehta, Nili, additional, Murrell, Matthew T., additional, Painter, Nathan, additional, Ricci, William, additional, Sbrollini, Kaitlyn, additional, Sharma, Rahul, additional, Steel, Peter A.D., additional, Steinkamp, Michele, additional, Weinberg, Roniel, additional, Wellman, David Stephenson, additional, Nader, Antoun, additional, Fitzgerald, Paul, additional, Ritz, Michaela, additional, Papp, Steven, additional, Bryson, Greg, additional, Craig, Alexandra, additional, Farhat, Cassandra, additional, Gammon, Braden, additional, Gofton, Wade, additional, Harris, Nicole, additional, Lalonde, Karl, additional, Liew, Allan, additional, Meulenkamp, Bradley, additional, Sonnenburg, Kendra, additional, Wai, Eugene, additional, Wilkin, Geoffrey, additional, Donegan, Derek, additional, Dinh, Cassandra, additional, Elkassabany, Nabil, additional, Horan, Annamarie, additional, Mehta, Samir, additional, Troxell, Karen, additional, Alderfer, Mary Ellen, additional, Brannen, Jason, additional, Cupitt, Christopher, additional, Gerhart, Stacy, additional, McLin, Renee, additional, Sheidy, Julie, additional, Yurick, Katherine, additional, Carson, Jeffrey, additional, Chen, Fei, additional, Dragert, Karen, additional, Kiss, Geza, additional, Malveaux, Halina, additional, McCloskey, Deborah, additional, Mellender, Scott, additional, Mungekar, Sagar S., additional, Noveck, Helaine, additional, Sagebien, Carlos, additional, Perlman, Barry, additional, Biby, Luat, additional, McKelvy, Gail, additional, Richards, Anna, additional, Azim, Syed, additional, Abola, Ramon, additional, Ayala, Brittney, additional, Halper, Darcy, additional, Mavarez, Ana, additional, Choi, Stephen, additional, Awad, Imad, additional, Flynn, Brendan, additional, Henry, Patrick, additional, Jenkinson, Richard, additional, Kaustov, Lilia, additional, Lappin, Elizabeth, additional, McHardy, Paul, additional, Singh, Amara, additional, Hauck, Ellen, additional, Donnelly, Joanne, additional, Gonzalez, Meera, additional, Haydel, Christopher, additional, Livelsberger, Jon, additional, Pazionis, Theresa, additional, Slattery, Bridget, additional, Vazquez-Trejo, Maritza, additional, Schwenk, Eric, additional, Baratta, Jaime, additional, Deiling, Brittany, additional, Deschamps, Laura, additional, Glick, Michael, additional, Katz, Daniel, additional, Krieg, James, additional, Lessin, Jennifer, additional, Torjman, Marc, additional, Chin, Ki Jinn, additional, Jin, Rongyu, additional, Salpeter, Mary Jane, additional, Powell, Mark, additional, Simmons, Jeffrey, additional, Lawson, Prentiss, additional, Kukreja, Promil, additional, Graves, Shanna, additional, Sturdivant, Adam, additional, Bryant, Ayesha, additional, Crump, Sandra Joyce, additional, Dillane, Derek, additional, Taylor, Michael, additional, Verrier, Michelle, additional, Applegate, Richard, additional, Arias, Ana, additional, Pineiro, Natasha, additional, Uppington, Jeffrey, additional, Wolinsky, Phillip, additional, Sappenfield, Joshua, additional, Gunnett, Amy, additional, Hagen, Jennifer, additional, Harris, Sara, additional, Hollen, Kevin, additional, Holloway, Brian, additional, Horodyski, Mary Beth, additional, Pogue, Trevor, additional, Ramani, Ramachandran, additional, Smith, Cameron, additional, Woods, Anna, additional, Warrick, Matthew, additional, Flynn, Kelly, additional, Mongan, Paul, additional, Ranganath, Yatish, additional, Fernholz, Sean, additional, Ingersoll-Weng, Esperanza, additional, Marian, Anil, additional, Seering, Melinda, additional, Sibenaller, Zita, additional, Stout, Lori, additional, Wagner, Allison, additional, Walter, Alicia, additional, Wong, Cynthia, additional, Magaziner, Jay, additional, Orwig, Denise, additional, Brown, Trina, additional, Dattilo, Jim, additional, Ellenberg, Susan, additional, Feng, Rui, additional, Fleisher, Lee, additional, Gaskins, Lakisha, additional, Goud, Maithri, additional, Helker, Chris, additional, Mezenghie, Lydia, additional, Montgomery, Brittany, additional, Preston, Peter, additional, Stephens, Alisa, additional, Schwartz, J. Sanford, additional, Tierney, Ann, additional, Weber, Ramona, additional, Chelly, Jacques, additional, Goel, Shiv, additional, Goncz, Wende, additional, Kawabe, Touichi, additional, Khetarpal, Sharad, additional, King, Kevin, additional, Kunkel, Frank, additional, Luke, Charles, additional, Monroe, Amy, additional, Shick, Vladislav, additional, Silipo, Anthony, additional, Stehle, Caroline, additional, Szabo, Katherine, additional, Yennam, Sudhakar, additional, Hoeft, Mark, additional, Breidenstein, Max, additional, Dominick, Timothy, additional, Friend, Alexander, additional, Mathews, Donald, additional, Lennertz, Richard, additional, Akere, Helen, additional, Balweg, Tyler, additional, Bo, Amber, additional, Doro, Christopher, additional, Goodspeed, David, additional, Lang, Gerald, additional, Parker, Maggie, additional, Rettammel, Amy, additional, Roth, Mary, additional, Sanders, Robert, additional, White, Marissa, additional, Whiting, Paul, additional, Allen, Brian, additional, Baker, Tracie, additional, Craven, Debra, additional, McEvoy, Matt, additional, Turnbo, Teresa, additional, Kates, Stephen, additional, Morgan, Melanie, additional, Willoughby, Teresa, additional, Weigel, Wade, additional, Auyong, David, additional, Fox, Ellie, additional, Welsh, Tina, additional, Cusson, Bruce, additional, Dobson, Sean, additional, Edwards, Christopher, additional, Harris, Lynette, additional, Henshaw, Daryl, additional, Johnson, Kathleen, additional, McKinney, Glen, additional, Miller, Scott, additional, Reynolds, Jon, additional, Turner, Jimmy, additional, VanEenenaam, David, additional, Weller, Robert, additional, Akhtar, Shamsuddin, additional, Blessing, Marcelle, additional, Johnson, Chanel, additional, Kampp, Michael, additional, Kunze, Kimberly, additional, Li, Jinlei, additional, O'Connor, Mary, additional, and Treggiari, Miriam, additional

Published
2021
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25. Comparison of the Analgesic Duration of 0.5% Bupivacaine With 1:200,000 Epinephrine Versus 0.5% Ropivacaine Versus 1% Ropivacaine for Low-Volume Ultrasound-Guided Interscalene Brachial Plexus Block: A Randomized Controlled Trial

Author

Safa, Ben, primary, Flynn, Brendan, additional, McHardy, Paul G., additional, Kiss, Alex, additional, Haslam, Lynn, additional, Henry, Patrick D., additional, Kaustov, Lilia, additional, and Choi, Stephen, additional

Published
2021
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26. Single-stranded DNA mimicry in the p53 transactivation domain interaction with replication protein A

Author

Bochkareva, Elena, Kaustov, Lilia, Ayed, Ayeda, Yi, Gwan-Su, Lu, Ying, Pineda-Lucena, Antonio, Liao, Jack C.C., Okorokov, Andrei L., Milner, Jo, Arrowsmith, Cheryl H., and Bochkarev, Alexey

Subjects
DNA-ligand interactions -- Research, Proteins -- Research, Science and technology
Abstract

One of many protein-protein interactions modulated upon DNA damage is that of the single-stranded DNA-binding protein, replication protein A (RPA), with the p53 tumor suppressor. Here we report the crystal structure of RPA residues 1-120 (RPA70N) bound to the N-terminal transactivation domain of p53 (residues 37-57; p53N) and, by using NMR spectroscopy, characterize two mechanisms by which the RPA/p53 interaction can be modulated. RPA70N forms an oligonucleotide/oligosaccharide-binding fold, similar to that previously observed for the ssDNA-binding domains of RPA. In contrast, the N-terminal p53 transactivation domain is largely disordered in solution, but residues 37-57 fold into two amphipathic helices, H1 and H2, upon binding with RPA70N. The H2 helix of p53 structurally mimics the binding of ssDNA to the oligonucleotide/oligosaccharide-binding fold. NMR experiments confirmed that both ssDNA and an acidic peptide mimicking a phosphorylated form of RPA32N can independently compete the acidic p53N out of the binding site. Taken together, our data suggest a mechanism for DNA damage signaling that can explain a threshold response to DNA damage. DNA binding | protein-protein interaction | structural analysis ssDNA mimicry

Published
2005

27. Inhibition mode of a bisubstrate inhibitor of KDO8P synthase: a frequency-selective REDOR solid-state and solution NMR characterization

Author

Kaustov, Lilia, Kababya, Shifi, Belakhov, Valery, Baasov, Timor, Shoham, Yuval, and Schmidt, Asher

Subjects
Molecular structure -- Research, Phosphatases -- Research, Phosphatases -- Mechanical properties, Nuclear magnetic resonance -- Usage, Chemistry
Abstract

The mode of inhibition of mechanism-based inhibitor (2,Ki = 0.4 (mu)M) of 3-deoxy-D-manno-2-octulosonate-8-phosphate synthase (KDO8PS), which is designed to mimic the combined key features of its natural substrates arabinose-5-phosphate and phoshoenolpyruvate into a single molecule, is investigated. A novel frequency-selective rotational-echo double-resonance (REDOR) experiment is developed and applied.

Published
2003

29. Structural and mechanistic investigation of 3-deoxy-D-manno-octulosonate-8-phosphate synthase by solid-state REDOR NMR

Author

Kaustov, Lilia, Kababya Shifi, Du, Schoucheng, Baasov, Timor, Gropper, Sara, Shoham, Yuval, and Schmidt, Asher

Subjects
Enzymes -- Structure-activity relationship, Amino acids -- Structure-activity relationships, Carbohydrate metabolism -- Research, Endotoxins -- Physiological aspects, Binding sites (Biochemistry) -- Analysis, Nuclear magnetic resonance spectroscopy -- Usage, Biological sciences, Chemistry
Abstract

Results demonstrate that the enzyme binds phosphoenolpyruvate and arabinose-5-phosphate via arginine and lysine residues by different mechanisms. Data reveal that the active site residues for phosphoenolpyruvate are structurally rigid residues lysine-138 and arginine-168 wheras arginine 63, which binds to arabinose-5-phosphate is flexible.

Published
2000

30. Spin transition in a manganese(III) porphyrin cation radical, its transformation to a dichloromanganese(IV) porphyrin, and chlorination of hydrocarbons by the latter

Author

Kaustov, Lilia, Tal, Merav E., Shames, Alexander I., and Gross, Zeev

Subjects
Porphyrins -- Research, Magnetic fields -- Research, Chemistry
Abstract

Chemical research shows that the spin transition of oxidized metalloporphyrin manganese porphyrin cation radical (TMP.+)MnIII(ClO4)2 is modified at high temperatures from S = 3/2 to S = 5/2. Other iron porphyrins exhibit similar characteristics. The production of (TMP)Mn(super 1V)(Cl)2 involves the ligand exchange of perchlorato ligands in (TMP.+)Mn1V(Cl)2.

Published
1997

31. The MLL1 trimeric catalytic complex is a dynamic conformational ensemble stabilized by multiple weak interactions

Author

Kaustov, Lilia, primary, Lemak, Alexander, additional, Wu, Hong, additional, Faini, Marco, additional, Fan, Lixin, additional, Fang, Xianyang, additional, Zeng, Hong, additional, Duan, Shili, additional, Allali-Hassani, Abdellah, additional, Li, Fengling, additional, Wei, Yong, additional, Vedadi, Masoud, additional, Aebersold, Ruedi, additional, Wang, Yunxing, additional, Houliston, Scott, additional, and Arrowsmith, Cheryl H, additional

Published
2019
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32. MLL1 minimal catalytic complex is a dynamic conformational ensemble susceptible to pharmacological allosteric disruption

Author

Kaustov, Lilia, primary, Lemak, Alexander, additional, Wu, Hong, additional, Faini, Marco, additional, Houliston, Scott, additional, Fan, Lixin, additional, Fang, Xianyang, additional, Zeng, Hong, additional, Duan, Shili, additional, Allali-Hassani, Abdellah, additional, Vedadi, Masoud, additional, Aebersold, Ruedi, additional, Wang, Yunxing, additional, and Arrowsmith, Cheryl H., additional

Published
2018
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33. Conformational dynamics of the TTD–PHD histone reader module of the UHRF1 epigenetic regulator reveals multiple histone-binding states, allosteric regulation, and druggability

Author

Houliston, R. Scott, primary, Lemak, Alexander, additional, Iqbal, Aman, additional, Ivanochko, Danton, additional, Duan, Shili, additional, Kaustov, Lilia, additional, Ong, Michelle S., additional, Fan, Lixin, additional, Senisterra, Guillermo, additional, Brown, Peter J., additional, Wang, Yun-Xing, additional, and Arrowsmith, Cheryl H., additional

Published
2017
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35. Pre-hospital hypothermia is associated with transfusion risk after traumatic injury

Author

Tien, Homer, MacDonald, Russell, Jivraj, Naheed K., Kaustov, Lilia, Hao, Kennedy Ning, Strauss, Rachel, Callum, Jeannie, Tien, Homer, and Alam, Asim Q.

Abstract

ABSTRACTObjectivesIn traumatically injured patients, excessive blood loss necessitating the transfusion of red blood cell (RBC) units is common. Indicators of early RBC transfusion in the pre-hospital setting are needed. This study aims to evaluate the association between hypothermia (<36°C) and transfusion risk within the first 24 hours after arrival to hospital for a traumatic injury.MethodsWe completed an audit of all traumatically injured patients who had emergent surgery at a single tertiary care center between 2010 and 2014. Using multivariable logistic regression analysis, we evaluated the association between pre-hospital hypothermia and transfusion of ≥1 unit of RBC within 24 hours of arrival to the trauma bay.ResultsOf the 703 patients included to evaluate the association between hypothermia and RBC transfusion, 203 patients (29%) required a transfusion within 24 hours. After controlling for important confounding variables, including age, sex, coagulopathy (platelets and INR), hemoglobin, and vital signs (blood pressure and heart rate), hypothermia was associated with a 68% increased odds of transfusion in multivariable analysis (OR: 1.68; 95% CI: 1.11-2.56).ConclusionsHypothermia is strongly associated with RBC transfusion in a cohort of trauma patients requiring emergent surgery. This finding highlights the importance of early measures of temperature after traumatic injury and the need for intervention trials to determine if strategies to mitigate the risk of hypothermia will decrease the risk of transfusion and other morbidities.

Published
2020
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36. Hemi-methylated DNA regulates DNA methylation inheritance through allosteric activation of H3 ubiquitylation by UHRF1

Author

Harrison, Joseph S, primary, Cornett, Evan M, additional, Goldfarb, Dennis, additional, DaRosa, Paul A, additional, Li, Zimeng M, additional, Yan, Feng, additional, Dickson, Bradley M, additional, Guo, Angela H, additional, Cantu, Daniel V, additional, Kaustov, Lilia, additional, Brown, Peter J, additional, Arrowsmith, Cheryl H, additional, Erie, Dorothy A, additional, Major, Michael B, additional, Klevit, Rachel E, additional, Krajewski, Krzysztof, additional, Kuhlman, Brian, additional, Strahl, Brian D, additional, and Rothbart, Scott B, additional

Published
2016
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37. Author response: Hemi-methylated DNA regulates DNA methylation inheritance through allosteric activation of H3 ubiquitylation by UHRF1

Author

Harrison, Joseph S, primary, Cornett, Evan M, additional, Goldfarb, Dennis, additional, DaRosa, Paul A, additional, Li, Zimeng M, additional, Yan, Feng, additional, Dickson, Bradley M, additional, Guo, Angela H, additional, Cantu, Daniel V, additional, Kaustov, Lilia, additional, Brown, Peter J, additional, Arrowsmith, Cheryl H, additional, Erie, Dorothy A, additional, Major, Michael B, additional, Klevit, Rachel E, additional, Krajewski, Krzysztof, additional, Kuhlman, Brian, additional, Strahl, Brian D, additional, and Rothbart, Scott B, additional

Published
2016
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40. Direct identification of enzyme active site residues by solid-state REDOR NMR: application to KDO8P synthase

Author

Kaustov, Lilia, Kababya, Shifi, Schoucheng Du, Baasov, Timor, Gropper, Sara, Shoham, Yuval, and Schmidt, Asher

Subjects
Chemical inhibitors -- Research, Enzymes -- Research, Ligands -- Research, Nuclear magnetic resonance -- Usage, Proteins -- Research, Chemistry
Abstract

Research is presented describing the identification of active site residues of the enzyme 3-deoxy-D-manno-2-octulosonate-8-phosphate synthase by the use of rotational-echo double-resonance solid-state NMR experiments.

Published
2000

42. Recognition and Specificity Determinants of the Human Cbx Chromodomains

Author

Kaustov, Lilia, primary, Ouyang, Hui, additional, Amaya, Maria, additional, Lemak, Alexander, additional, Nady, Nataliya, additional, Duan, Shili, additional, Wasney, Gregory A., additional, Li, Zhihong, additional, Vedadi, Masoud, additional, Schapira, Matthieu, additional, Min, Jinrong, additional, and Arrowsmith, Cheryl H., additional

Published
2011
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43. CUL7 Is a Novel Antiapoptotic Oncogene

Author

Kim, Sam S., primary, Shago, Mary, additional, Kaustov, Lilia, additional, Boutros, Paul C., additional, Clendening, James W., additional, Sheng, Yi, additional, Trentin, Grace A., additional, Barsyte-Lovejoy, Dalia, additional, Mao, Daniel Y.L., additional, Kay, Robert, additional, Jurisica, Igor, additional, Arrowsmith, Cheryl H., additional, and Penn, Linda Z., additional

Published
2007
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48. Binding of the natural substrates and products to KDO8P synthase: 31P and 13C solution NMR characterization

Author

Kaustov, Lilia, Baasov, Timor, and Schmidt, Asher

Subjects
*PROTONS, *NUCLEAR magnetic resonance
Abstract

Proton decoupled 31P and 13C solution NMR experiments were applied to mixtures of 3-deoxy-d-manno-2-octulosonate-8-phosphate (KDO8P) synthase, with each of its natural substrates, phosphoenolpyruvate and arabinose-5-phosphate (ASP), and product KDO8P to identify the formation of the enzyme–substrate and enzyme–product complexes. Effects arising from ligand interactions with the enzyme are reported via chemical shifts and line broadening with respect to those of the free ligands in solution, depending on the strength and dynamics of binding under thermodynamic equilibrium conditions. The characterization was done both at low and high field spectrometers, 200 and 500 MHz (1H frequencies), and in cases of 31P NMR measurements, it was demonstrated that only the low field spectrometer is capable of providing direct experimental evidence on the enzyme–ligand interactions. Since both the substrate A5P and the product KDO8P exhibit multiple anomeric forms in solution, evidence for the preference of recognition and binding of particular forms is sought. [Copyright &y& Elsevier]

Published
2003
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49. Inhibition Mode of Bisubstrate Inhibitor of KDO8P Synthase: A Frequency-Selective REDOR Solid-State and Solution NMR Characterization.

Author

Kaustov, Lilia, Kababya, Shifi, Belakhov, Valery, Baasov, Timor, Shoham, Yuval, and Schmidt, Asher

Subjects
*PHOSPHORUS compounds, *CHEMICAL inhibitors
Abstract

In this report the mode of inhibition of mechanism-based inhibitor (2, K[sub i] = 0.4 µM) of 3-deoxyD-manno-2-octulosonate-8-phosphate synthase (KDO8PS), which was designed to mimic the combined key features of its natural substrates arabinose-5-phosphate (A5P) and phoshoenolpyruvate (PEP) into a single molecule, was investigated. Our earlier solid-state NMR observations identified the inhibitor to bind in a way that partly mimics ASP, while the phosphonate moiety of its PEP-mimicking part exhibits no interactions with enzyme residues. This result was apparently in disagreement with the competitive inhibition of 2 against PEP and with the later solved crystal structure of KDO8PS-2 binary complex identifying the interactions of its PEP-mimicking part with the enzyme residues that were not detected by solid-state NMR. To solve this discrepancy, further solid-state REDOR NMR and [sup 31]P solution NMR experiments were applied to a variety of enzyme complexes with the substrates and inhibitor. In particular, a novel frequency-selective REDOR experiment was developed and applied. Integration of the solution and solid-state NMR data clearly demonstrates that under conditions of stoichiometric enzyme-ligand ratio at thermodynamic equilibrium (a) PEP binding is unperturbed by the presence of 2 and (b) both PEP and 2 can bind simultaneously to the synthase, i.e., form a ternary complex with PEP occupying its own subsite and 2 occupying A5P's subsite. The latter observation suggests that under the conditions used in our NMR measurements, the inhibition pattern of 2 against PEP should have a mixed type character. Furthermore, the NMR data directly demonstrate the distinction between the relative binding strength of the two moieties of 2: enzyme interactions with PEP-mimicking moiety are much weaker than those with the A5P moiety. This observation is in agreement with KDO8PS-2 crystal structure showing only remote contacts of the phosphonate due to large structural... [ABSTRACT FROM AUTHOR]

Published
2003
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50. The MLL1 trimeric catalytic complex is a dynamic conformational ensemble stabilized by multiple weak interactions

Author

Kaustov, Lilia, Lemak, Alexander, Wu, Hong, Faini, Marco, Fan, Lixin, Fang, Xianyang, Zeng, Hong, Duan, Shili, Allali-Hassani, Abdellah, Li, Fengling, Wei, Yong, Vedadi, Masoud, Aebersold, Ruedi, Wang, Yunxing, Houliston, Scott, and Arrowsmith, Cheryl H.

Subjects
3. Good health
Abstract

Histone H3K4 methylation is an epigenetic mark associated with actively transcribed genes. This modification is catalyzed by the mixed lineage leukaemia (MLL) family of histone methyltransferases including MLL1, MLL2, MLL3, MLL4, SET1A and SET1B. The catalytic activity of this family is dependent on interactions with additional conserved proteins, but the structural basis for subunit assembly and the mechanism of regulation is not well understood. We used a hybrid methods approach to study the assembly and biochemical function of the minimally active MLL1 complex (MLL1, WDR5 and RbBP5). A combination of small angle X-ray scattering, cross-linking mass spectrometry, nuclear magnetic resonance spectroscopy and computational modeling were used to generate a dynamic ensemble model in which subunits are assembled via multiple weak interaction sites. We identified a new interaction site between the MLL1 SET domain and the WD40 β-propeller domain of RbBP5, and demonstrate the susceptibility of the catalytic function of the complex to disruption of individual interaction sites., Nucleic Acids Research, 47 (17), ISSN:1362-4962, ISSN:0301-5610

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