Search

Your search keyword '"Kaufmann, Svenja"' showing total 7 results
Start Over Author "Kaufmann, Svenja"
7 results on '"Kaufmann, Svenja"'

1. Defective mitochondrial COX1 translation due to loss of COX14 function triggers ROS-induced inflammation in mouse liver

Author

Aich, Abhishek, Boshnakovska, Angela, Witte, Steffen, Gall, Tanja, Unthan-Fechner, Kerstin, Yousefi, Roya, Chowdhury, Arpita, Dahal, Drishan, Methi, Aditi, Kaufmann, Svenja, Silbern, Ivan, Prochazka, Jan, Nichtova, Zuzana, Palkova, Marcela, Raishbrook, Miles, Koubkova, Gizela, Sedlacek, Radislav, Tröder, Simon E., Zevnik, Branko, Riedel, Dietmar, Michanski, Susann, Möbius, Wiebke, Ströbel, Philipp, Lüchtenborg, Christian, Giavalisco, Patrick, Urlaub, Henning, Fischer, Andre, Brügger, Britta, Jakobs, Stefan, and Rehling, Peter

Published
2024
Full Text
View/download PDF

3. Human Anti-fungal Th17 Immunity and Pathology Rely on Cross-Reactivity against Candida albicans

Author

Bacher, Petra, Hohnstein, Thordis, Beerbaum, Eva, Röcker, Marie, Blango, Matthew G., Kaufmann, Svenja, Röhmel, Jobst, Eschenhagen, Patience, Grehn, Claudia, Seidel, Kathrin, Rickerts, Volker, Lozza, Laura, Stervbo, Ulrik, Nienen, Mikalai, Babel, Nina, Milleck, Julia, Assenmacher, Mario, Cornely, Oliver A., Ziegler, Maren, Wisplinghoff, Hilmar, Heine, Guido, Worm, Margitta, Siegmund, Britta, Maul, Jochen, Creutz, Petra, Tabeling, Christoph, Ruwwe-Glösenkamp, Christoph, Sander, Leif E., Knosalla, Christoph, Brunke, Sascha, Hube, Bernhard, Kniemeyer, Olaf, Brakhage, Axel A., Schwarz, Carsten, and Scheffold, Alexander

Published
2019
Full Text
View/download PDF

4. Control of intracellular pH and bicarbonate by CO2 diffusion into human sperm.

Author

Grahn, Elena, Kaufmann, Svenja V., Askarova, Malika, Ninov, Momchil, Welp, Luisa M., Berger, Thomas K., Urlaub, Henning, and Kaupp, U.Benjamin

Subjects
BIOLOGICAL transport, BICARBONATE ions, SEA urchins, SPERM motility, PROTEIN expression, SODIUM channels, LIQUID chromatography-mass spectrometry
Abstract

The reaction of CO2 with H2O to form bicarbonate (HCO3−) and H+ controls sperm motility and fertilization via HCO3−-stimulated cAMP synthesis. A complex network of signaling proteins participates in this reaction. Here, we identify key players that regulate intracellular pH (pHi) and HCO3− in human sperm by quantitative mass spectrometry (MS) and kinetic patch-clamp fluorometry. The resting pHi is set by amiloride-sensitive Na+/H+ exchange. The sperm-specific putative Na+/H+ exchanger SLC9C1, unlike its sea urchin homologue, is not gated by voltage or cAMP. Transporters and channels implied in HCO3− transport are not detected, and may be present at copy numbers < 10 molecules/sperm cell. Instead, HCO3− is produced by diffusion of CO2 into cells and readjustment of the CO2/HCO3−/H+ equilibrium. The proton channel Hv1 may serve as a unidirectional valve that blunts the acidification ensuing from HCO3− synthesis. This work provides a new framework for the study of male infertility. Bicarbonate (HCO3−) is critical in sperm for stimulation of cAMP synthesis during fertilization, though there is dispute over how HCO3− is transported into sperm. Here the authors use limit-of-detection LC/MS to characterize sperm protein expression and show that HCO3− is produced from CO2 diffusion into sperm rather than active transport. [ABSTRACT FROM AUTHOR]

Published
2023
Full Text
View/download PDF

7. [Untitled]

Author

Hoffmann, Markus, Kaufmann, Svenja Victoria, Fischer, Carina, Maurer, Wiebke, Moldenhauer, Anna-Sophie, and Pöhlmann, Stefan

Subjects
glycoprotein, Microbiology (medical), Proteases, lcsh:Medicine, medicine.disease_cause, Article, Cathepsin B, Virus, Cathepsin L, 03 medical and health sciences, Viral entry, ebola, medicine, Immunology and Allergy, Ebola, cathepsin, Molecular Biology, 030304 developmental biology, Cathepsin, 0303 health sciences, Ebola virus, General Immunology and Microbiology, biology, 030306 microbiology, lcsh:R, biology.organism_classification, Virology, 3. Good health, Infectious Diseases, Vesicular stomatitis virus, biology.protein
Abstract

Ebola virus (EBOV) infection can cause severe and frequently fatal disease in human patients. The EBOV glycoprotein (GP) mediates viral entry into host cells. For this, GP depends on priming by the pH-dependent endolysosomal cysteine proteases cathepsin B (CatB) and, to a lesser degree, cathepsin L (CatL), at least in most cell culture systems. However, there is limited information on whether and how EBOV-GP can acquire resistance to CatB/L inhibitors. Here, we addressed this question using replication-competent vesicular stomatitis virus bearing EBOV-GP. Five passages of this virus in the presence of the CatB/CatL inhibitor MDL28170 were su cient to select resistant viral variants and sequencing revealed that all GP sequences contained a V37A mutation, which, in the context of native GP, is located in the base of the GP surface unit. In addition, some GP sequences harbored mutation S195R in the receptor-binding domain. Finally, mutational analysis demonstrated that V37A but not S195R conferred resistance against MDL28170 and other CatB/CatL inhibitors. Collectively, a single amino acid substitution in GP is su cient to confer resistance against CatB/CatL inhibitors, suggesting that usage of CatB/CatL inhibitors for antiviral therapy may rapidly select for resistant viral variants. Open-Access-Publikationsfonds 2019 peerReviewed

Catalog

Books, media, physical & digital resources
See catalog results